专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20190161775A1 Two-Stage Production of Higher Alcohols 审中-公开
公开(公告)号：US20190161775A1
公开(公告)日：2019-05-30
申请号：US16264009
申请日：2019-01-31
申请人： Easel Biotechnologies, LLC
发明人： Yi-Xin HUO , Kwang Myung CHO
IPC分类号： C12P7/16 , C12M1/00 , C12P39/00 , C12M1/40
CPC分类号： C12P7/16 , C12M21/12 , C12M21/18 , C12M23/58 , C12P7/56 , C12P13/06 , C12P13/08 , C12P39/00 , C12Y101/01001 , C12Y104/01009 , Y02E50/10
摘要： Methods and systems for the production of alcohols are described. A two stage process is utilized, where fermentation in a first stage produces an intermediate product, such as an amino acid or organic acid, from a carbon containing feedstock. A second stage produces alcohol by fermentation of this intermediate product.

2. 发明申请

US20170232043A1 BACTERIA ENGINEERED TO TREAT DISORDERS INVOLVING THE CATABOLISM OF A BRANCHED CHAIN AMINO ACID 审中-公开
公开(公告)号：US20170232043A1
公开(公告)日：2017-08-17
申请号：US15379445
申请日：2016-12-14
申请人： Synlogic, Inc.
发明人： Dean Falb , Paul F. Miller , Yves Millet , Vincent M. Isabella , Jonathan W. Kotula , Alex Tucker
IPC分类号： A61K35/74 , A61K38/44 , C12N9/88 , C12N15/70 , C12N9/10 , C12N9/04 , C07K14/245 , A61K9/00 , C12N9/06
CPC分类号： A61K35/74 , A61K9/0019 , A61K9/0053 , A61K38/44 , A61K38/443 , C07K14/245 , C12N9/00 , C12N9/0006 , C12N9/0016 , C12N9/0022 , C12N9/1096 , C12N9/88 , C12N15/52 , C12N15/70 , C12Y101/01 , C12Y101/01001 , C12Y101/01086 , C12Y104/01009 , C12Y104/03002 , C12Y206/01042 , C12Y401/01 , C12Y401/01001 , Y02A50/473
摘要： The present disclosure provides recombinant bacterial cells that have been engineered with genetic circuitry which allow the recombinant bacterial cells to sense a patient's internal environment and respond by turning an engineered metabolic pathway on or off. When turned on, the recombinant bacterial cells complete all of the steps in a metabolic pathway to achieve a therapeutic effect in a host subject. These recombinant bacterial cells are designed to drive therapeutic effects throughout the body of a host from a point of origin of the microbiome. Specifically, the present disclosure provides recombinant bacterial cells comprising a heterologous gene encoding a branched chain amino acid catabolism enzyme. The disclosure further provides pharmaceutical compositions comprising the recombinant bacteria, and methods for treating disorders involving the catabolism of branched chain amino acids using the pharmaceutical compositions disclosed herein.

3. 发明申请

US20170159029A1 OXIDASE, POLYNUCLEOTIDE THAT CODES FOR SAME, AND USE THEREOF 审中-公开
公开(公告)号：US20170159029A1
公开(公告)日：2017-06-08
申请号：US15325287
申请日：2015-07-02
申请人： Sumitomo Chemical Company, Limited
发明人： Hiroyuki ASAKO
IPC分类号： C12N9/06 , C12P13/06 , C12P13/12
CPC分类号： C12N9/0006 , C07K2319/21 , C12N5/10 , C12N9/0016 , C12N15/09 , C12P13/04 , C12P13/06 , C12P13/12 , C12Y104/01009
摘要： A polynucleotide encoding an amino acid sequence represented by SEQ ID NO: 1 or 3; a protein having an amino acid sequence represented by SEQ ID NO: 1 or 3; and a method for producing an L-α-amino acid compound are provided. The method includes a step (1) of reacting the protein described herein with an α-hydroxycarboxylic acid compound to obtain a corresponding α-oxocarboxylic acid compound; and a step of reacting a protein having the ability to aminate an α-oxocarboxylic acid compound and convert the same into a corresponding L-α-amino acid compound with the α-oxocarboxylic acid compound obtained in step (1) to obtain a corresponding L-α-amino acid compound.

4. 发明申请

US20160281119A1 PROCESSES USING AMINO ACID DEHYDROGENASES AND KETOREDUCTASE-BASED COFACTOR REGENERATING SYSTEM 审中-公开
公开(公告)号：US20160281119A1
公开(公告)日：2016-09-29
申请号：US15184242
申请日：2016-06-16
申请人： Codexis, Inc.
发明人： Fabien Louis Cabirol , Steven J. Collier , Thomas Daussmann , Naga K. Modukuru
IPC分类号： C12P13/06 , C12N9/06 , C12N9/04 , C12P41/00
CPC分类号： C12P41/007 , C12N9/0004 , C12N9/0006 , C12N9/0016 , C12P7/40 , C12P13/04 , C12P13/06 , C12Y101/00 , C12Y101/01 , C12Y104/01009 , C12Y104/99001
摘要： The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.

5. 发明申请

US20170191097A1 OXIDASE, POLYNUCLEOTIDE ENCODING SAME, AND USE THEREOF 审中-公开
公开(公告)号：US20170191097A1
公开(公告)日：2017-07-06
申请号：US15325190
申请日：2015-07-02
申请人： Sumitomo Chemical Company, Limited
发明人： Hiroyuki ASAKO
IPC分类号： C12P13/12 , C12P11/00 , C12N9/06
CPC分类号： C12P13/12 , C12N5/10 , C12N9/0016 , C12N15/09 , C12P7/40 , C12P9/00 , C12P11/00 , C12P13/04 , C12Y102/01002 , C12Y104/01009 , C12Y111/01006
摘要： Isolated oxidases, isolated polynucleotides encoding the oxidases, and methods of using the oxidases to produce α-oxocarboxylic acid compounds or L-α-amino acid compounds are described.

6. 发明申请

US20160222359A1 Modified Leucine Dehydrogenase 有权
公开(公告)号：US20160222359A1
公开(公告)日：2016-08-04
申请号：US15133599
申请日：2016-04-20
申请人： AJINOMOTO CO., INC.
发明人： Wataru Hoshino , Yuya Kodama , Toshimi Mizukoshi , Uno Tagami
IPC分类号： C12N9/06 , C12P13/06 , C12Q1/32
CPC分类号： C12N9/0016 , C07K2319/02 , C12P13/06 , C12P13/08 , C12Q1/32 , C12Y104/01009 , G01N33/6812 , G01N2333/90616
摘要： The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

7. 发明授权

US09080192B2 Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system 有权
标题翻译：使用氨基酸脱氢酶和基于酮还原酶的辅因子再生系统的方法
公开(公告)号：US09080192B2
公开(公告)日：2015-07-14
申请号：US13577772
申请日：2011-02-08
申请人： Fabien L. Cabirol , Steven J. Collier , Thomas Daussmann , Naga Modukuru
发明人： Fabien L. Cabirol , Steven J. Collier , Thomas Daussmann , Naga Modukuru
IPC分类号： C12P13/04 , C12N9/02 , C12P41/00
CPC分类号： C12P41/007 , C12N9/0004 , C12N9/0006 , C12N9/0016 , C12P7/40 , C12P13/04 , C12P13/06 , C12Y101/00 , C12Y101/01 , C12Y104/01009 , C12Y104/99001
摘要： The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
摘要翻译：本公开涉及氨基酸脱氢酶与包含酮还原酶的辅因子再生系统的组合的用途。在具体实施方案中，该方法可用于使用亮氨酸脱氢酶制备L-叔亮氨酸。

8. 发明申请

US20140227747A1 Coryneform Bacterium Transformant and Process for Producing Valine Using the Same 有权
标题翻译：棒状细菌转化体及使用其生产缬氨酸的方法
公开(公告)号：US20140227747A1
公开(公告)日：2014-08-14
申请号：US14238527
申请日：2012-08-21
申请人： Hideaki Yukawa , Masayaki Inui
发明人： Hideaki Yukawa , Masayaki Inui
IPC分类号： C12N15/77 , C12P13/08
CPC分类号： C12N15/77 , C12N9/0006 , C12N9/0016 , C12N9/1022 , C12P13/08 , C12Y101/01086 , C12Y104/01009 , C12Y202/01006 , Y02P20/52
摘要： A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host. (a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof. (b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof. (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or an analog thereof.
摘要翻译：将一种或多种以下DNA（a），（b）和（c）引入作为宿主的棒状细菌获得的转化体。（a）编码源自谷氨酸棒杆菌的乙酰羟酸合酶的DNA，其在DNA编码的氨基酸序列或其类似物中具有将位置156处的甘氨酸变为谷氨酸（G156E）的突变。（b）编码源自谷氨酸棒杆菌的乙酰羟酸异构体还原酶的DNA，其具有将第34位的丝氨酸改变为甘氨酸（S34G），将第48位的亮氨酸变为谷氨酸（L48E），将第49位的精氨酸变为苯丙氨酸（R49F），其由DNA编码的氨基酸序列或其类似物。（c）编码源自嗜裂杆菌属的亮氨酸脱氢酶的DNA，或其类似物。

9. 发明授权

US07642073B2 Method for reductive amination of a ketone using a mutated enzyme 有权
标题翻译：使用突变酶还原胺化酮的方法
公开(公告)号：US07642073B2
公开(公告)日：2010-01-05
申请号：US11786054
申请日：2007-04-09
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12P13/00
CPC分类号： C12Y104/01009 , C12N9/0006 , C12N9/0016 , C12N9/0018 , C12P13/04
摘要： Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amine from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination of the target ketone under conditions sufficient to permit the formation of the desired amine to thereby produce the amine.
摘要翻译：提供了使用突变酶化学转化化合物的方法，更具体地，涉及一种从目标酮制备胺的方法。所述方法包括产生在足以允许形成所需胺以产生胺的条件下催化目标酮的还原胺化的突变酶。

10. 发明申请

US20170145451A1 Redox Self-Sufficient Biocatalytic Amination of Alcohols 审中-公开
公开(公告)号：US20170145451A1
公开(公告)日：2017-05-25
申请号：US15323515
申请日：2015-07-02
申请人： BASF SE
发明人： Kai-Uwe Baldenius , Michael Breuer , Klaus Ditrich , Vaidotas Navickas , Francesco Mutti , Tanja Knaus , Nicholas Turner
IPC分类号： C12P13/00 , C12N9/04 , C12N9/06
CPC分类号： C12P13/001 , C12N9/0006 , C12N9/0016 , C12N9/0018 , C12Y101/01001 , C12Y104/01009 , C12Y104/0102
摘要： The present invention relates to a novel biocatalytic method for the production of primary and secondary amines, comprising coupled enzymatic oxidation and reduction processes simultaneously regenerating the required cofactor; making use of two enzymes—i.e. an alcohol dehydrogenase and an amine dehydrogenase—operating simultaneously in a one pot process (i.e. biocatalytic cascade). Furthermore, the overall process is redox neutral, since the hydride generated from the first oxidative step is internally recycled in the second reductive step. The invention also relates to recombinant expression systems and microorganisms procuring the required enzyme activities; and bioreactors for performing such methods.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式