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    • 1. 发明申请
    • MICROCHIP TREATMENT DEVICE FOR MACHINE TOOL
    • US20230102942A1
    • 2023-03-30
    • US17909980
    • 2021-03-16
    • DN SOLUTIONS CO., LTD.
    • Eun Soo SEO
    • B23Q11/10B01D36/04B01D21/26
    • A flushing duct is installed on both sides of a table of the machine tool in a longitudinal direction of the table, a slope duct is inclinedly disposed below the flushing duct toward the coolant tank, each bottom surface of the flushing duct and the slope duct is formed with a V-shaped inclined surface and a streamlined bending portion to make flows of the coolant containing the microchips smooth, and a coolant supply valve is installed at a rear end of the flushing duct to facilitate flows of the coolant being discharged by supplying additional coolant. Further, a coolant tank is installed below the slope duct, a filter is installed in the coolant tank for filtering the microchips in the coolant. The coolant containing the microchips is transported through a return pump to a centrifugal separator device where the microchips contained in the coolant are centrifugally separated. A refined coolant through centrifugal separation is supplied to the machine tool, thereby preventing abnormal wear due to penetration of the microchips into rotating and sliding portions of the machine tool.
    • 3. 发明授权
    • Methods for isolating target cells from blood
    • US11604191B2
    • 2023-03-14
    • US16556632
    • 2019-08-30
    • William BusaPhilip H. CoelhoJonathan EllisDalip Sethi
    • William BusaPhilip H. CoelhoJonathan EllisDalip Sethi
    • G01N33/569B01D21/26G01N33/543
    • Disclosed herein are methods for isolating target cells from blood, involving mixing in an open container an undiluted blood sample having a volume of 10 ml or less, and binding agents, wherein each binding agent comprises (A) a primary binding agent comprising an agent capable of binding to at least one cellular epitope on target cells in the undiluted blood sample, (B) a first linker bound to the primary binding agent, to generate binding agent-attached target cells in the undiluted blood sample; contacting the binding agent-attached target cells in the undiluted blood sample with a plurality of buoyant reagents that include a second linker capable of binding to the first linker to generate an undiluted buoyant reagent-attached target cell mixture; diluting the undiluted buoyant reagent-attached target cell mixture by at least 20% to produce a diluted buoyant reagent-attached target cell mixture; applying a vectorial force, such as centrifugal force, to the diluted buoyant reagent-attached target cell mixture to generate a stratified diluted buoyant reagent-attached target cell mixture; removing the buoyant reagent-attached target cells from the stratified diluted buoyant reagent-attached target cell mixture; and isolating the target cells from the buoyant reagent-attached target cells.