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    • 5. 发明授权
    • Fermentation method
    • 发酵法
    • US4181576A
    • 1980-01-01
    • US931636
    • 1978-08-07
    • Emil A. Malick
    • Emil A. Malick
    • C12M1/04C12B1/00
    • C12M23/06C12M29/06C12M29/18C12M47/00Y10S435/813
    • A fermentation apparatus of the loop flow type includes a tubular member forming a continuous or substantially continuous loop flow path defined by the interior surface of the tubular member. A heat exchanger surrounds a portion of the tubular member and is in heat transfer relation thereto and inside the tubular member at the point where the heat exchange means surrounds the tubular member mixing vanes or fins are secured to the tubular member to effect mixing of medium flowing through the tubular member. A spray nozzle is provided just upstream of the mixing vanes and is operable for discharge of medium into the flow path adjacent the heat exchange means. A pump is connected to the nozzle and pressurizes medium taken from the flow path and discharges same through the nozzle. Injectors open into the flow path adjacent the heat exchange means and are operable for introducing an oxygen-containing gas into the medium contained in the flow path. A phase separator has an inlet and outlet communicating with the flow path and is operable for receiving a portion of the medium for separating same into a gas phase and liquid phase and returning a portion of the liquid phase to the flow path.
    • 环流型的发酵装置包括形成由管状构件的内表面限定的连续或基本上连续的环路流动路径的管状构件。 热交换器围绕管状构件的一部分并且与管状构件的热传递关系,并且在热交换装置围绕管状构件的点处,混合叶片或翅片固定到管状构件上以实现流动的介质的混合 通过管状构件。 在混合叶片的正上方设置有喷嘴,并且可操作用于将介质排放到与热交换装置相邻的流动路径中。 泵连接到喷嘴并对从流动路径取出的介质加压,并通过喷嘴排出。 喷射器通向与热交换装置相邻的流路,并且可操作用于将含氧气体引入包含在流路中的介质中。 相分离器具有与流路连通的入口和出口,并且可操作用于接收用于将其分离成气相和液相的介质的一部分并将液相的一部分返回到流路。
    • 10. 发明授权
    • Stabilized liquid phosphate containing diagnostic compositions and
method of preparing same
    • 含有稳定的含磷酸液体的诊断组合物及其制备方法
    • US4132598A
    • 1979-01-02
    • US803036
    • 1977-06-03
    • Ivan E. Modrovich
    • Ivan E. Modrovich
    • C12Q1/42C12B1/00
    • C12Q1/42C12Q2334/10
    • A stabilized liquid phosphate containing diagnostic composition and generally a phosphate containing substrate used in the determination of enzymes such as alkaline phosphatase and acid phosphatase. The composition actually includes a buffer composition containing a magnesium ion for activation of the enzyme and a substrate composition, which are mixed at time of determination. The buffer composition is of the proper pH so that when the buffer composition and the substrate composition are mixed, the resultant mixture will adopt the pH of the buffer composition. The stabilizer which is employed is one that will prevent hydrolysis of the phosphate from the organic substrate. Effective stabilizers include phenol and phenolic compounds and imidazole and nitro alaphatic compounds having from one through six carbon atoms.
    • 含有稳定的含磷酸的含磷酸的诊断组合物和一般含有磷酸盐的底物,用于测定酶如碱性磷酸酶和酸性磷酸酶。 该组合物实际上包含含有用于活化酶的镁离子的缓冲组合物和在测定时混合的底物组合物。 缓冲剂组合物具有适当的pH,使得当缓冲组合物和底物组合物混合时,所得混合物将采用缓冲组合物的pH。 所用的稳定剂是防止磷酸盐从有机底物中水解的稳定剂。 有效的稳定剂包括苯酚和酚类化合物以及具有一至六个碳原子的咪唑和硝基化合物。