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1. 发明公开

KR1020040101793A 사람 에리트로포이에틴을 생산하는 형질전환 복제소 및이의 생산 방법 无效
标题翻译：转基因克隆生产人ERYTHROPOIETIN及其生产方法
公开(公告)号：KR1020040101793A
公开(公告)日：2004-12-03
申请号：KR1020030033582
申请日：2003-05-27
申请人： 재단법인서울대학교산학협력재단
发明人： 황우석 , 이병천 , 강성근 , 정의배 , 조종기 , 김순웅 , 박을순 , 장구 , 박희정
IPC分类号： C12N5/16
CPC分类号： C12N15/8771 , A01K67/0273 , A01K2227/101 , A01K2267/01 , A61D19/04 , C12N15/8509 , C12N2015/8518 , C12N2517/00
摘要： PURPOSE: Transgenic cloned cow producing human erythropoietin and a method for producing the same transgenic cloned cow are provided, which method allows economic and effective production of various biomedicines, and facilitates production of human erythropoietin in cow's milk. CONSTITUTION: The nuclear transfer embryo produced by a fusion of a cow-derived enucleated oocyte with a nucleus of a cow-derived somatic cell transfected with a gene encoding human erythropoietin is provided, wherein the cow is an adult; the nuclear transfer embryo is SNU-B4(KCTC 10450BP). The method for producing a transgenic cloned cow expressing human erythropoietin in the mammary gland comprises the steps of: (a) introducing a gene encoding human erythropoietin into a cow-derived somatic cell line to prepare a nuclear donor cell; (b) removing cumulus cells surrounding a recipient oocyte and then the cytoplasm including the first polar body from the oocyte to prepare a cow-derived enucleated recipient oocyte; (c) transferring the transfected nuclear donor cell into the enucleated recipient oocyte and carrying out cell fusion to generate nuclear transfer embryos; and (d) transplanting the nuclear transfer embryo into a surrogate mother cow to produce live offspring.
摘要翻译：目的：提供转基因克隆的牛产生人促红细胞生成素和生产相同转基因克隆牛的方法，该方法允许经济有效地生产各种生物医学，并促进牛奶中人促红细胞生成素的产生。构成：提供由牛衍生的去核卵母细胞与用编码人促红细胞生成素的基因转染的牛衍生的体细胞的细胞核融合而产生的核转移胚，其中，牛是成年人; 核转移胚是SNU-B4（KCTC 10450BP）。用于产生在乳腺中表达人促红细胞生成素的转基因克隆牛的方法包括以下步骤：（a）将编码人促红细胞生成素的基因导入牛衍生的体细胞系以制备核供体细胞; （b）去除卵母细胞周围的卵丘细胞，然后从卵母细胞中除去包含第一极体的细胞质，以制备牛衍生的去核受体卵母细胞; （c）将转染的核供体细胞转移到去核的受体卵母细胞中并进行细胞融合以产生核转移胚胎; 和（d）将核转移胚胎移植到替代母牛中以产生活的后代。

2. 发明公开

KR1020030033582A 지능형 누전 차단장치 无效
标题翻译：智能地漏泄漏装置
公开(公告)号：KR1020030033582A
公开(公告)日：2003-05-01
申请号：KR1020010065647
申请日：2001-10-24
申请人： 조영일 , 채기병
发明人： 조영일 , 채기병
IPC分类号： H01H83/02
摘要： PURPOSE: An intelligent earth leakage breaker apparatus is provided to achieve in-advance prevention against accident caused due to electric leakage, by compulsorily cutting off the power being supplied to the load upon detection of electric leakage. CONSTITUTION: An intelligent earth leakage breaker apparatus comprises a zero-current transition mounted on a power line connected to a load so as to detect the grounding current flowing along the power line; a controller IC for amplifying the grounding current detected by the zero-current transition, judging the electric leakage of the load by comparing the amplified grounding current with a preset reference level, and controlling SCR, LED and voice generating IC if it is judged as an electric leakage; a trip coil which is excited being interlocked with the SCR and compulsorily cutting off the power being supplied to the load upon occurrence of electric leakage; an LED which turns on by the control of the controller IC upon occurrence of electric leakage and displaying electric leakage state; and a voice generator IC which operates by the control of the controller IC upon occurrence of electric leakage and outputting voices for informing electric leakage state through a speaker.
摘要翻译：目的：提供一种智能漏电断路器装置，通过在检测到漏电时强制切断供给负载的电力，实现事先防止因漏电而引起的事故。构成：智能漏电断路器装置包括安装在连接到负载的电力线上的零电流转换，以便检测沿电力线流动的接地电流; 用于放大由零电流转换检测到的接地电流的控制器IC，通过将放大的接地电流与预设的参考电平进行比较来判断负载的漏电，并且如果判断为SCR，则控制SCR，LED和语音产生IC 漏电; 跳闸线圈被激励与SCR互锁，并且在发生漏电时强制切断供给负载的电力; LED发生漏电状态时通过控制器IC的控制而接通的LED，以及语音发生器IC，其通过控制器IC的控制而发生漏电并输出用于通过扬声器通知泄漏状态的语音来操作。

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