会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 2. 发明授权
    • Use of gel polymer for DNA extraction with organic solvents
    • 使用凝胶聚合物进行DNA萃取与有机溶剂
    • US5106966A
    • 1992-04-21
    • US381588
    • 1989-07-10
    • Stanley ThomasLowell TilzerRuben Moreno
    • Stanley ThomasLowell TilzerRuben Moreno
    • C12N15/10C12Q1/68
    • C12N15/1003C12Q1/6806Y10S435/803
    • The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a gel polymer, such as a polyester gel polymer to the mixture of sample and separating reagent followed by agitation via, e.g., centrifugation, the DNA and protein are separated, with the gel acting as a block to prevent contamination of the DNA phase by the protein. Higher yields of DNA are obtained as compared to methodologies where the gel is not used. Additionally, the problems associated with the physical contact of the solvents, which are frequently carcinogens, are avoided. Also taught are kits which can be used in connection with the inventive method.
    • 本发明描述了从样品中提取DNA的方法。 它包括使样品与分离试剂接触,分离试剂允许DNA和蛋白质的分化溶剂化。 通过将诸如聚酯凝胶聚合物的凝胶聚合物添加到样品和分离试剂的混合物中,然后通过例如离心进行搅拌,分离DNA和蛋白质,凝胶充当块,以防止DNA相的污染 由蛋白质。 与不使用凝胶的方法相比,获得更高的DNA产量。 此外,避免了与经常致癌的溶剂的物理接触相关的问题。 还教导了可以结合本发明的方法使用的试剂盒。