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序号	专利名	申请号	申请日	公开（公告）号	公开（公告）日	发明人
1	一种含二十二碳六烯酸油脂的无溶剂提取方法	CN201710539326.5	2017-07-04	CN107099561A	2017-08-29	左文路; 黄和; 胡学超; 任路静; 纪晓俊; 江凌; 陈可泉; 郝宁; 宋萍; 郭东升; 孙小曼
本发明公开了一种含二十二碳六烯酸（DHA）油脂的无溶剂提取方法。该方法包括：以裂殖壶菌为出发菌株，在液体培养基中连续发酵得到发酵液；向发酵液中添加氢氧化钠溶液调节pH；加热酶解罐；添加生物复合酶液进行破壁反应；离心分离酶解液获得富含二十二碳六烯酸油脂。通过本发明从裂殖壶菌中提取油脂，毛油提取率高达95％以上，得到的油脂酸价低，色泽浅，无溶剂残留，提取时间更短，提取成本更低。
2	一种同步去除氰、铬的深度处理电镀污水的方法	CN201610624755.8	2016-08-03	CN106116042B	2019-04-30	李为
本发明涉及一种同步去除氰、铬的深度处理电镀污水的方法，其包括如下步骤：（1）对电镀废水进行固液分离；（2）将步骤（1）获得液体通过微电解反应器；（3）将经过微电解反应器的废水pH调至10，然后加入次氯酸钠和氢氧化钠，反应后，调 pH值为6‑7，20min后加入絮凝剂，进行絮凝处理，然后进行静置沉淀，时间4‑6h，之后获得澄清上清液；（4）将步骤（3）处理获得的上清液排到微生物反应池，调节PH值为7－8，然后按照每立方米液体投加生物菌剂10g，静置一周。本发明方法提高处理水量和处理水质，降低运行费用，促进排放水质达到标准。
3	低密度蔬菜有机菌肥及其制备方法	CN202210046664.6	2022-01-17	CN114507096A	2022-05-17	苑学亮; 李莉; 扈保杰; 李虎申; 张岳华; 王怀珍; 刘永高; 李民厚; 刘明
本发明公开了低密度蔬菜有机菌肥及其制备方法，所述有机菌肥的密度为0.6～0.8g/cm3；所述有机菌肥包含：中微量营养元素0.5～1.5％、木质素磺酸锌0.05～0.2％、粉煤灰1～2％、硫酸铵0.5～0.8％、硫酸铝0.15～0.35%、硫酸亚铁0.15～0.45%，固定化白僵菌培养物1～1.5%、固定化绿僵菌培养物1～1.5%、复配微生物粉剂0.5～2%；其余为发酵有机物。其制备方法包括蘑菇渣进行收集、高温发酵、低温发酵、混料等步骤。本发明低密度蔬菜有机菌肥可解决蔬菜种植中化学肥料用量过度、土壤板结，造成高微生物定殖存活性能低的问题。
4	一种同步去除氰、铬的深度处理电镀污水的方法	CN201610624755.8	2016-08-03	CN106116042A	2016-11-16	李为
本发明涉及一种同步去除氰、铬的深度处理电镀污水的方法，其包括如下步骤：（1）对电镀废水进行固液分离；（2）将步骤（1）获得液体通过微电解反应器；（3）将经过微电解反应器的废水pH调至10，然后加入次氯酸钠和氢氧化钠，反应后，调pH值为6‑7，20min后加入絮凝剂，进行絮凝处理，然后进行静置沉淀，时间4‑6h，之后获得澄清上清液；（4）将步骤（3）处理获得的上清液排到微生物反应池，调节PH值为7－8，然后按照每立方米液体投加生物菌剂10g，静置一周。本发明方法提高处理水量和处理水质，降低运行费用，促进排放水质达到标准。
5	Hydrogen peroxide-forming sarcosine oxidase	US868262	1986-05-28	US4743549A	1988-05-10	Ulrich Mayr; Hans Mollering; Joachim Siedel; Hans Seidel
The present invention provides a hydrogen peroxide-forming sarcosine oxidase, wherein it is obtainable from Streptomycetaceae and at 25.degree. C. in 0.15 mol/liter potassium phosphate (pH 7.9), in the presence of surface-active substances, still shows after 2 days an activity of at least 40% of the initial activity.
6		DE69025405	1990-08-13	DE69025405T2	1996-09-05	KRUUS ILKKA; LAINE JAAKKO; KOLJONEN MARJA

7		BR9007655	1990-08-13	BR9007655A	1992-07-07	KRUUS ILKKA; LAINE JAAKO; KOLJONEN MARJA

8		PT9498490	1990-08-13	PT94984A	1991-04-18	IIKKA KRUUS; JAAKKO LAINE; MARJA KOLJONEN

9	DNA SEGMENT FUNCTIONAL AS STARTING POINT OF REPRODUCTION OF STREPTOMYCES AND RELATIVE GENUS	JP1946691	1991-01-18	JPH08168379A	1996-07-02	MAAGARETSUTO MATSUKENJII BAROU

10		JP17116186	1986-07-21	JPH0634731B2	1994-05-11	OKUMURA MASAKAZU; II SHIGERU; ICHIKAWA SHINYA

11		JP20529384	1984-09-29	JPH0362718B2	1991-09-26	SAKANO KATSUICHI; OOSHIMA MASASHI

12		JP51087790	1990-08-13	JPH04507268A	1992-12-17

13		JP50834688	1988-09-26	JPH03500366A	1991-01-31

14	PRODUCTION OF UNSATURATED WAX ESTER	JP17116186	1986-07-21	JPS6328396A	1988-02-06	OKUMURA MASAKAZU; II SHIGERU; ICHIKAWA SHINYA
PURPOSE:To efficiently obtain the titled ester, having a high content of unsaturated double bonds and useful as a substitute raw material for sperm whale oil at a low cost, by cultivating a microorganism capable of assimilating an n-paraffin in a culture medium containing an unsaturated fatty acid and, as necessary, the n-paraffin. CONSTITUTION:(A) A microorganism of the genus Acinetobacter, Saccharomyces, etc., capable of assimilating an n-paraffin is inoculated into (B) a culture medium consisting of (a) a nitrogen compound, e.g. ammonium sulfate, etc., assimilable by the microorganism, (b) an inorganic salt, e.g. sodium phosphate, ferrous sulfate, etc., (c), as necessary, an organism growth promoting substance, e.g. vitamin, (d) an unsaturated fatty acid, e.g. oleic acid, linoleic acid, etc., as a carbon source and a lower organic acid, e.g. acetic acid, etc., as the second carbon source, and, as necessary, a 10-24C n-paraffin, etc., and aerobically cultivated at 20-40 deg.C while shaking to afford the titled ester.
15		JP27866584	1984-12-25	JP2579747B2	1997-02-12	YAMADA MASAAKI; FURUYA TAIJI; YAMAYOSHI MICHIKO; NOTAKE MITSUE; YAMAGISHI JUNICHI

16	NOVEL DNA TO COKE HUMAN INTERLEUKIN 1	JP27866584	1984-12-25	JPS61149092A	1986-07-07	YAMADA MASAAKI; FURUYA TAIJI; YAMAYOSHI MICHIKO; NOTAKE MITSUE; YAMAGISHI JUNICHI
PURPOSE:To obtain human interleukin 1 having physiological activity such as immune response, etc., by cultivating a bacterium transformed with a plasmid integrated with a specific cloned DNA. CONSTITUTION:Culture preparation using a human leukemia cell as an origin is carried out, to give cloned DNA to code human interleukin 1. Then, it is integrated into a plasmied to give a transformed vector, which is transduced into E.coliHB101, to give a transformant. Then, the transformant is precultivated, part of it is inoculated into modified medium M9 such as Casamino acid, etc., cultivated at 37 deg.C for about one hour, a given amount of indole-3-acetic acid is added to the culture mixture, and it is further cultivated for about 24hr. Then, the culture solution is centrifuged, molds are collected, the molds are resuspended in a buffer solution, freezing in a dry ice and/or ethanol bath and melting at about 37 deg.C are repeated about 6 times. Then, the solution is centrifuged to give a supernatant solution, which is treated to polypeptide of human interleukin 1.
17		JP3371172	1972-04-04	JPS4899390A	1973-12-15

18		NO920569	1992-02-13	NO920569L	1992-02-13	KRUUS ILKKA; LAINE JAAKKO; KOLJONEN MARJA

19		FI920609	1992-02-13	FI920609A0	1992-02-13	KRUUS ILKKA; LAINE JAAKKO; KOLJONEN MARJA

20	ENDO-XYLANASE FROM ACTINOMYCETES	NZ22642088	1988-09-30	NZ226420A	1991-02-26	HARI GOPAL VARTAK; KULBHUSHAN BALWANT BASTAWDE

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