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    • 53. 发明授权
    • Method of liquid nitrogen surface vitrification
    • 液氮表面玻璃化方法
    • US08633023B2
    • 2014-01-21
    • US13818072
    • 2011-08-17
    • Fuliang DuJie XuJuan Moreno
    • Fuliang DuJie XuJuan Moreno
    • C12N5/07C12N5/073C12N5/075A01N1/02C12N5/02
    • A01N1/02A01N1/0221A01N1/0226A01N1/0236A01N1/0278A01N1/0284C12N5/0604C12N5/0609
    • A method of liquid nitrogen surface vitrification requiring an embryo washed in a rinsing medium, then incubated in a base medium and incubated in a hold medium before being washed in a vitrification medium and produced into a vitrification droplet (270). For forming the droplet, vitrification medium (210), an intermediary fluid such as air, followed by vitrification medium containing at least one embryo (250) are aspirated into the channel. The vitrification droplet consequently can contain an air bubble (220). The vitrification droplet can be produced from an instrument with a channel and dropped directly into liquid phase nitrogen producing a vitrified droplet. The vitrified droplet can be stored in cryo-vessels, and warmed for revitalization of biological function of vitrified biological cell mass or tissues, such as oocytes and/or embryos.
    • 一种液氮表面玻璃化的方法,其需要在漂洗介质中洗涤胚胎,然后在基础培养基中温育并在保持培养基中孵育,然后在玻璃化培养基中洗涤并生成玻璃化液滴(270)。 为了形成液滴,玻璃化介质(210),诸如空气的中间流体,随后含有至少一个胚胎(250)的玻璃化介质被吸入通道中。 玻璃化微滴因此可以包含气泡(220)。 玻璃化液滴可以由具有通道的仪器产生并直接滴入液相氮中,产生玻璃化液滴。 玻璃化液滴可以储存在低温容器中,并加热使玻璃化生物细胞团或组织如卵母细胞和/或胚胎的生物功能恢复活力。
    • 56. 发明申请
    • METHOD OF LIQUID NITROGEN SURFACE VITRIFICATION
    • 液氮表面维修方法
    • US20130157362A1
    • 2013-06-20
    • US13818072
    • 2011-08-17
    • Fuliang DuJie XuJuan Moreno
    • Fuliang DuJie XuJuan Moreno
    • C12N5/02
    • A01N1/02A01N1/0221A01N1/0226A01N1/0236A01N1/0278A01N1/0284C12N5/0604C12N5/0609
    • A method of liquid nitrogen surface vitrification requiring an embryo washed in a rinsing medium, then incubated in a base medium and incubated in a hold medium before being washed in a vitrification medium and produced into a vitrification droplet (270). For forming the droplet, vitrification medium (210), an intermediary fluid such as air, followed by vitrification medium containing at least one embryo (250) are aspirated into the channel. The vitrification droplet consequently can contain an air bubble (220). The vitrification droplet can be produced from an instrument with a channel and dropped directly into liquid phase nitrogen producing a vitrified droplet. The vitrified droplet can be stored in cryo-vessels, and warmed for revitalization of biological function of vitrified biological cell mass or tissues, such as oocytes and/or embryos.
    • 一种液氮表面玻璃化的方法,其需要在漂洗介质中洗涤胚胎,然后在基础培养基中温育并在保持培养基中孵育,然后在玻璃化培养基中洗涤并生成玻璃化液滴(270)。 为了形成液滴,玻璃化介质(210),诸如空气的中间流体,随后含有至少一个胚胎(250)的玻璃化介质被吸入通道中。 玻璃化微滴因此可以包含气泡(220)。 玻璃化液滴可以由具有通道的仪器产生并直接滴入液相氮中,产生玻璃化液滴。 玻璃化液滴可以储存在低温容器中,并加热使玻璃化生物细胞团或组织如卵母细胞和/或胚胎的生物功能恢复活力。
    • 58. 发明申请
    • CELL IDENTIFYING APPARATUS AND CELL IDENTIFYING METHOD
    • 细胞鉴定装置和细胞鉴定方法
    • US20120171700A1
    • 2012-07-05
    • US13342245
    • 2012-01-03
    • Toru TakahashiKen TsukiiJie Xu
    • Toru TakahashiKen TsukiiJie Xu
    • G01N21/64C12M1/34
    • G01N15/14
    • A cell identifying apparatus includes a capillary through which a sample flow passes, an irradiating unit that irradiates irradiation light, a light receiving unit that receives transmitted light and outputs a transmitted light signal based on the transmitted light, a measuring unit that receives the transmitted light signal output from the light receiving unit and measures the change of the transmitted light signal caused by cells, an identifying unit that identifies whether the cells are peripheral blood cells on the basis of a value of an extreme point in the change of the transmitted light signal, the change being caused by the cells and measured by the measuring unit, a sorting unit that sorts the cells in a predetermined vessel, on the basis of the identification result of the identifying unit, and a supplying unit that supplies the sample flow to the capillary.
    • 细胞识别装置包括:样品流过的毛细管,照射照射光的照射单元,接收透射光的光接收单元,并且基于透射光输出透射光信号;测量单元,其接收透射光 信号从光接收单元输出,并测量由小区引起的透射光信号的变化;识别单元,其基于透射光信号的变化中的极值点来识别细胞是否为外周血细胞 所述改变是由所述单元引起的并且由所述测量单元测量的分类单元,其基于所述识别单元的识别结果对预定容器中的单元进行排序;以及供给单元,其将所述样本流提供给所述单元 毛细管。