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1. 发明申请

US20120329125A1 GROUP CULTURE SYSTEM AND METHOD WITH HELPER EMBRYOS 审中-公开
标题翻译：组织文化系统和方法与帮助EMBRYOS
公开(公告)号：US20120329125A1
公开(公告)日：2012-12-27
申请号：US13168707
申请日：2011-06-24
申请人： Fuliang Du , Giorgio Antonio Presicce , Juan Moreno , Jie Xu , Shinn-Chin Wu
发明人： Fuliang Du , Giorgio Antonio Presicce , Juan Moreno , Jie Xu , Shinn-Chin Wu
IPC分类号： C12N11/10 , C12N11/04 , C12N5/00 , C12M3/00
CPC分类号： C12N5/0604 , C12M21/06 , C12M23/00 , C12M25/00 , C12M35/00 , C12N2502/02 , C12N2533/76
摘要： Methods and systems for physically separating helper embryos from desired embryos in a group culturing technique in order to maintain the pedigree and genetic information of the desired embryos from different species, including cattle and human, and to provide the developmental benefits of group culturing. The separation of the groups of embryos can be the result of embedding one group of embryos in a gel or solid, or the groups can be physically separated by a membrane or other structure.
摘要翻译：在组织培养技术中物理分离辅助胚胎与期望的胚胎的方法和系统，以便保持来自不同物种（包括牛和人）的所需胚胎的谱系和遗传信息，并提供组培养的发育益处。胚胎组的分离可以是将一组胚胎嵌入凝胶或固体中的结果，或者这些基团可以通过膜或其它结构物理分离。

2. 发明申请

US20120202286A1 rbLIF PROTEIN FOR USE IN EMBRYONIC STEM CELL CULTURES 审中-公开
标题翻译： rbLIF蛋白用于胚胎干细胞培养
公开(公告)号：US20120202286A1
公开(公告)日：2012-08-09
申请号：US13451268
申请日：2012-04-19
申请人： Jie Xu , Jerry Yang , Xiuchun Tian , Fuliang Du , Yinghong Ma
发明人： Jie Xu , Jerry Yang , Xiuchun Tian , Fuliang Du , Yinghong Ma
IPC分类号： C12N5/0735 , C12N5/02
CPC分类号： C07K14/5415 , C12N2501/235
摘要： Provided herein methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell on a rabbit feeder cell layer, wherein the rabbit feeder layer expresses a leukemia inhibitory factor (LIF) protein. Also provided are methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell in a culture medium comprising rabbit leukemia inhibitory factor (rbLIF) protein. Also provided are polynucleotide and amino acid sequences of a rabbit leukemia inhibitory factor protein (rbLIF).
摘要翻译：本发明提供将胚胎干细胞培养物维持在未分化状态的方法，其中所述方法包括在兔饲养细胞层上培养胚胎干细胞，其中所述兔饲养层表达白血病抑制因子（LIF）蛋白。还提供了将胚胎干细胞培养物维持在未分化状态的方法，其中所述方法包括在包含兔白血病抑制因子（rbLIF）蛋白的培养基中培养胚胎干细胞。还提供了兔白血病抑制因子蛋白（rbLIF）的多核苷酸和氨基酸序列。

3. 发明申请

US20100227403A1 rbLIF PROTEIN FOR USE IN EMBRYONIC STEM CELL CULTURES 审中-公开
标题翻译： rbLIF蛋白用于胚胎干细胞培养
公开(公告)号：US20100227403A1
公开(公告)日：2010-09-09
申请号：US12637660
申请日：2009-12-14
申请人： Jie Xu , Jerry Yang , Xiuchun Tian , Fuliang Du , Yinghong Ma
发明人： Jie Xu , Jerry Yang , Xiuchun Tian , Fuliang Du , Yinghong Ma
IPC分类号： C12N5/0735 , C07K14/00 , C07H21/04
CPC分类号： C07K14/5415 , C12N2501/235
摘要： Provided herein methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell on a rabbit feeder cell layer, wherein the rabbit feeder layer expresses a leukemia inhibitory factor (LIF) protein. Also provided are methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell in a culture medium comprising rabbit leukemia inhibitory factor (rbLIF) protein. Also provided are polynucleotide and amino acid sequences of a rabbit leukemia inhibitory factor protein (rbLIF).
摘要翻译：本发明提供将胚胎干细胞培养物维持在未分化状态的方法，其中所述方法包括在兔饲养细胞层上培养胚胎干细胞，其中所述兔饲养层表达白血病抑制因子（LIF）蛋白。还提供了将胚胎干细胞培养物维持在未分化状态的方法，其中所述方法包括在包含兔白血病抑制因子（rbLIF）蛋白的培养基中培养胚胎干细胞。还提供了兔白血病抑制因子蛋白（rbLIF）的多核苷酸和氨基酸序列。

4. 发明授权

US08633023B2 Method of liquid nitrogen surface vitrification 有权
标题翻译：液氮表面玻璃化方法
公开(公告)号：US08633023B2
公开(公告)日：2014-01-21
申请号：US13818072
申请日：2011-08-17
申请人： Fuliang Du , Jie Xu , Juan Moreno
发明人： Fuliang Du , Jie Xu , Juan Moreno
IPC分类号： C12N5/07 , C12N5/073 , C12N5/075 , A01N1/02 , C12N5/02
CPC分类号： A01N1/02 , A01N1/0221 , A01N1/0226 , A01N1/0236 , A01N1/0278 , A01N1/0284 , C12N5/0604 , C12N5/0609
摘要： A method of liquid nitrogen surface vitrification requiring an embryo washed in a rinsing medium, then incubated in a base medium and incubated in a hold medium before being washed in a vitrification medium and produced into a vitrification droplet (270). For forming the droplet, vitrification medium (210), an intermediary fluid such as air, followed by vitrification medium containing at least one embryo (250) are aspirated into the channel. The vitrification droplet consequently can contain an air bubble (220). The vitrification droplet can be produced from an instrument with a channel and dropped directly into liquid phase nitrogen producing a vitrified droplet. The vitrified droplet can be stored in cryo-vessels, and warmed for revitalization of biological function of vitrified biological cell mass or tissues, such as oocytes and/or embryos.
摘要翻译：一种液氮表面玻璃化的方法，其需要在漂洗介质中洗涤胚胎，然后在基础培养基中温育并在保持培养基中孵育，然后在玻璃化培养基中洗涤并生成玻璃化液滴（270）。为了形成液滴，玻璃化介质（210），诸如空气的中间流体，随后含有至少一个胚胎（250）的玻璃化介质被吸入通道中。玻璃化微滴因此可以包含气泡（220）。玻璃化液滴可以由具有通道的仪器产生并直接滴入液相氮中，产生玻璃化液滴。玻璃化液滴可以储存在低温容器中，并加热使玻璃化生物细胞团或组织如卵母细胞和/或胚胎的生物功能恢复活力。

5. 发明申请

US20130157362A1 METHOD OF LIQUID NITROGEN SURFACE VITRIFICATION 有权
标题翻译：液氮表面维修方法
公开(公告)号：US20130157362A1
公开(公告)日：2013-06-20
申请号：US13818072
申请日：2011-08-17
申请人： Fuliang Du , Jie Xu , Juan Moreno
发明人： Fuliang Du , Jie Xu , Juan Moreno
IPC分类号： C12N5/02
CPC分类号： A01N1/02 , A01N1/0221 , A01N1/0226 , A01N1/0236 , A01N1/0278 , A01N1/0284 , C12N5/0604 , C12N5/0609
摘要： A method of liquid nitrogen surface vitrification requiring an embryo washed in a rinsing medium, then incubated in a base medium and incubated in a hold medium before being washed in a vitrification medium and produced into a vitrification droplet (270). For forming the droplet, vitrification medium (210), an intermediary fluid such as air, followed by vitrification medium containing at least one embryo (250) are aspirated into the channel. The vitrification droplet consequently can contain an air bubble (220). The vitrification droplet can be produced from an instrument with a channel and dropped directly into liquid phase nitrogen producing a vitrified droplet. The vitrified droplet can be stored in cryo-vessels, and warmed for revitalization of biological function of vitrified biological cell mass or tissues, such as oocytes and/or embryos.
摘要翻译：一种液氮表面玻璃化的方法，其需要在漂洗介质中洗涤胚胎，然后在基础培养基中温育并在保持培养基中孵育，然后在玻璃化培养基中洗涤并生成玻璃化液滴（270）。为了形成液滴，玻璃化介质（210），诸如空气的中间流体，随后含有至少一个胚胎（250）的玻璃化介质被吸入通道中。玻璃化微滴因此可以包含气泡（220）。玻璃化液滴可以由具有通道的仪器产生并直接滴入液相氮中，产生玻璃化液滴。玻璃化液滴可以储存在低温容器中，并加热使玻璃化生物细胞团或组织如卵母细胞和/或胚胎的生物功能恢复活力。

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