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1. 发明授权

US5785968A Anti-feline calicivirus recombinant antibody and gene fragment encoding the same 失效
标题翻译：抗猫杯状病毒重组抗体和编码相同的基因片段
公开(公告)号：US5785968A
公开(公告)日：1998-07-28
申请号：US24253
申请日：1993-03-01
申请人： Kazuhiko Kimachi , Hiroaki Maeda , Kiyoto Nishiyama , Sachio Tokiyoshi , Yukinobu Tohya , Takeshi Mikami
发明人： Kazuhiko Kimachi , Hiroaki Maeda , Kiyoto Nishiyama , Sachio Tokiyoshi , Yukinobu Tohya , Takeshi Mikami
IPC分类号： A61K38/00 , C07K16/10 , C12N15/13 , C12P21/08 , C07H21/04 , A61K39/395 , C07H16/00
CPC分类号： C07K16/1045 , A61K38/00
摘要： Anti-FCV (feline calicivirus) feline-type recombinant antibody effective for treatment, prevention and diagnosis of FCV infection and a gene fragment useful for preparation of said antibody are provided. Cell line 1D7 capable of producing a mouse monoclonal antibody having an excellent FCV-neutralizing activity was constructed and a gene fragment coding for the V region in charge of the FCV-specific binding of said antibody was obtained. This gene fragment and the gene coding for the constant region of the feline antibody are used to give a chimeric anti-FCV recombinant antibody. The obtained recombinant antibody is a novel antibody and is useful for the diagnosis, treatment and prevention of feline virus infections, particularly feline calicivirus infection, with high safety in administration into cats.
摘要翻译：提供了有效治疗，预防和诊断FCV感染的抗FCV（猫杯状病毒）猫科动物重组抗体和用于制备所述抗体的基因片段。构建能够产生具有优异的FCV中和活性的小鼠单克隆抗体的细胞系1D7，得到编码负责所述抗体的FCV特异性结合的V区的基因片段。使用该基因片段和编码猫抗体恒定区的基因得到嵌合抗FCV重组抗体。获得的重组抗体是一种新的抗体，可用于诊断，治疗和预防猫病毒感染，特别是猫杯状病毒感染，对猫的施用具有高度的安全性。

2. 发明申请

US20070166331A1 Modified seb and prophylactics/remedies for immunopathy containing the same 失效
标题翻译：用于含有其的免疫疾病的修饰型和预防药物/补救剂
公开(公告)号：US20070166331A1
公开(公告)日：2007-07-19
申请号：US10551263
申请日：2003-03-28
申请人： Toshihiro Nakashima , Takumi Sasaki , Kazuhiko Kimachi , Shigeki Kuwata , Tsukasa Nishihara , Atsuko Sakata , Masao Ohkuchi , Tomoyuki Koshi , Toshiyuki Edano
发明人： Toshihiro Nakashima , Takumi Sasaki , Kazuhiko Kimachi , Shigeki Kuwata , Tsukasa Nishihara , Atsuko Sakata , Masao Ohkuchi , Tomoyuki Koshi , Toshiyuki Edano
IPC分类号： A61K39/09 , C07K14/31
CPC分类号： A61K38/164 , C07K14/31 , C07K16/1271
摘要： A novel prophylactic/remedy for immunopathy is provided which is not neutralized by a neutralizing antibody to Staphylococcal enterotoxin B (SEB), known as one of superantigens, and may effectively act as a superantigen. A modified SEB having a reduced reactivity with a neutralizing antibody to SEB (anti-SEB antibody) and a prophylactic/remedy for immunopathy comprising as an active ingredient said modified SEB. The modified SEB of the present invention may be prepared with the evolutionary molecular engineering technique by introducing amino acid substitution in the amino acid sequence of SEB, especially at an epitope recognition site of the anti-SEB antibody in the amino acid sequence of SEB.
摘要翻译：提供一种免疫病的新型预防/治疗方法，其不被被称为超抗原之一的葡萄球菌肠毒素B（SEB）的中和抗体中和，并且可以有效地作为超抗原。与SEB中和抗体（抗SEB抗体）具有降低的反应性的修饰SEB和用于免疫病的预防/治疗方法，其包含所述经修饰的SEB作为活性成分。本发明的经修饰的SEB可以通过在SEB的氨基酸序列中引入氨基酸取代，特别是在SEB的氨基酸序列中的抗SEB抗体的表位识别位点处进行进化分子工程技术来制备。

3. 发明授权

US5880274A Cat-mouse heterohybridoma and gene fragment coding for constant region of feline immunoglobulin 失效
标题翻译：猫鼠异种杂交瘤和编码猫免疫球蛋白恒定区的基因片段
公开(公告)号：US5880274A
公开(公告)日：1999-03-09
申请号：US559947
申请日：1995-11-17
申请人： Hiroaki Maeda , Yasuyuki Eda , Kazuhiko Kimachi , Yoichi Ono , Sachio Tokiyoshi
发明人： Hiroaki Maeda , Yasuyuki Eda , Kazuhiko Kimachi , Yoichi Ono , Sachio Tokiyoshi
IPC分类号： C07K16/00 , C07K16/08 , C07K16/46 , C12N5/20 , C12N5/26 , C12N15/13 , C12P21/08 , C07H21/04 , C12N5/00 , C12N15/00
CPC分类号： C12N5/166 , C07K16/00 , C07K16/081 , C07K16/462 , C07K2317/24 , C07K2319/00
摘要： A Gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of feline immunoglobulin .lambda. chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of feline immunoglobulin .kappa. chain; a gene fragment which comprises a DNA sequence coding for the constant region of feline immunoglobulin .gamma. chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-cat chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a feline immunoglobulin wherein the latter gene fragment is linked to the 3' site of the former gene fragment; a polypeptide of a mouse-cat chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule coding for an amino acid sequence of the mouse-cat chimeric antibody is incorporated; a cat-mouse heterohybridoma which produces feline immunoglobulin; and a process for preparing a feline immunoglobulin gene.
摘要翻译：一种基因片段，其包含编码猫免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 一种基因片段，其包含编码猫免疫球蛋白κ链的恒定区的氨基酸序列的DNA序列; 包含编码猫免疫球蛋白γ链恒定区的DNA序列的基因片段; 编码小鼠-cat嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段的猫免疫球蛋白，其中后一个基因片段连接到前一个基因片段的3'位点; 将从编码小鼠-cat嵌合抗体的氨基酸序列的所述重组DNA分子并入的细胞的表达载体转化的细胞中表达的小鼠-cat嵌合抗体的多肽; 产生猫免疫球蛋白的猫 - 鼠异种杂交瘤; 以及制备猫免疫球蛋白基因的方法。

4. 发明授权

US5852183A Dog-mouse heterohybridoma and gene fragment coding for constant region of canine immunoglobulins 失效
标题翻译：犬小鼠异源杂交瘤和编码犬免疫球蛋白恒定区的基因片段
公开(公告)号：US5852183A
公开(公告)日：1998-12-22
申请号：US646981
申请日：1996-05-08
申请人： Hiroaki Maeda , Yasuyuki Eda , Kazuhiko Kimachi , Yoichi Ono , Sachio Tokiyoshi
发明人： Hiroaki Maeda , Yasuyuki Eda , Kazuhiko Kimachi , Yoichi Ono , Sachio Tokiyoshi
IPC分类号： A61K39/00 , C07K16/46 , C12N5/26 , C12N15/13 , C07H2/04 , C07H21/02
CPC分类号： C07K16/462 , A61K39/00 , C07K2317/24
摘要： A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin lambda chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin kappa chain; a gene fragment which comprises a DNA sequence coding for the constant region of canine immunoglobulin gamma chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a canine immunoglobulin wherein the latter gene fragment; a polypeptide of a mouse-dog chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule cording for an amino acid sequence of the mouse-dog chimeric antibody is incorporated; a dog-mouse heterohybridoma which produces canine immunoglobulin; and a process for preparing a canine immunoglobulin gene.
摘要翻译：一种基因片段，其包含编码犬免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 基因片段，其包含编码犬免疫球蛋白κ链恒定区的氨基酸序列的DNA序列; 包含编码犬免疫球蛋白γ链的恒定区的DNA序列的基因片段; 编码小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段的犬免疫球蛋白，其中后者基因片段; 从用细胞表达载体转化的细胞中表达小鼠 - 狗嵌合抗体的多肽，其中并入所述针对小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子; 产生犬免疫球蛋白的狗 - 小鼠异源杂交瘤; 以及制备犬免疫球蛋白基因的方法。

5. 发明授权

US08226958B2 Modified SEB and prophylactics/remedies for immunopathy containing the same 失效
标题翻译：修饰的SEB和预防药物/含有其的免疫疾病的补救措施
公开(公告)号：US08226958B2
公开(公告)日：2012-07-24
申请号：US10551263
申请日：2004-03-25
申请人： Toshihiro Nakashima , Takumi Sasaki , Kazuhiko Kimachi , Shigeki Kuwata , Tsukasa Nishihara , Atsuko Sakata , Masao Ohkuchi , Tomoyuki Koshi , Toshiyuki Edano
发明人： Toshihiro Nakashima , Takumi Sasaki , Kazuhiko Kimachi , Shigeki Kuwata , Tsukasa Nishihara , Atsuko Sakata , Masao Ohkuchi , Tomoyuki Koshi , Toshiyuki Edano
IPC分类号： A61K39/00 , A61K39/02 , A61K39/085 , C07K1/00 , C07K14/00 , C07K17/00
CPC分类号： A61K38/164 , C07K14/31 , C07K16/1271
摘要： A novel prophylactic/remedy for immunopathy is provided which is not neutralized by a neutralizing antibody to Staphylococcal enterotoxin B (SEB), known as one of superantigens, and may effectively act as a superantigen. A modified SEB having a reduced reactivity with a neutralizing antibody to SEB (anti-SEB antibody) and a prophylactic/remedy for immunopathy comprising as an active ingredient said modified SEB. The modified SEB of the present invention may be prepared with the evolutionary molecular engineering technique by introducing amino acid substitution in the amino acid sequence of SEB, especially at an epitope recognition site of the anti-SEB antibody in the amino acid sequence of SEB.
摘要翻译：提供一种免疫病的新型预防/治疗方法，其不被被称为超抗原之一的葡萄球菌肠毒素B（SEB）的中和抗体中和，并且可以有效地作为超抗原。与SEB中和抗体（抗SEB抗体）具有降低的反应性的修饰SEB和用于免疫病的预防/治疗方法，其包含所述经修饰的SEB作为活性成分。本发明的经修饰的SEB可以通过在SEB的氨基酸序列中引入氨基酸取代，特别是在SEB的氨基酸序列中的抗SEB抗体的表位识别位点处进行进化分子工程技术来制备。

6. 发明授权

US6114143A Anti-HIV monoclonal antibody 失效
标题翻译：抗HIV单克隆抗体
公开(公告)号：US6114143A
公开(公告)日：2000-09-05
申请号：US513968
申请日：1995-09-11
申请人： Yasuyuki Eda , Hiroaki Maeda , Keiichi Makizumi , Kouichi Shiosaki , Kiyoshi Osatomi , Kazuhiko Kimachi , Hirofumi Higuchi , Sachio Tokiyoshi
发明人： Yasuyuki Eda , Hiroaki Maeda , Keiichi Makizumi , Kouichi Shiosaki , Kiyoshi Osatomi , Kazuhiko Kimachi , Hirofumi Higuchi , Sachio Tokiyoshi
IPC分类号： A61K38/00 , C07K16/10 , C12P21/04 , C12P21/08 , C12N15/09 , A61K39/395 , C12P11/00
CPC分类号： C07K16/1063 , A61K38/00 , C07K2317/24
摘要： A monoclonal antibody useful for clinical application which recognizes the conserved region of V3-PND region of glycoprotein antigen having a molecular weight of about 1.2.times.10.sup.5 daltons (gp120) on a coating membrane of human immunodeficiency virus (HIV) and which has an ability to neutralize a broad range of various HIV variants, or a fragment thereof, and the chimeric and humanized antibodies derived therefrom are provided. By using as an immunogen a plurality of peptides having PND-Tip region containing the highly conserved GPGR sequence within PND of HIV gp120, a monoclonal antibody having a neutralizing activity to many HIV variants can be prepared. By transplanting the gene fragment coding for the variable region of said monoclonal antibody or complementarity determining region (CDR) of said region to a human antibody gene, a chimeric antibody or a reshaped antibody having an anti-HIV neutralizing activity which are effective for clinical application can be obtained.
摘要翻译： PCT No.PCT / JP94 / 00371 Sec。 371 1995年9月11日第 102（e）1995年9月11日PCT PCT 1994年3月9日PCT公布。公开号WO94 / 20632 日期1994年9月15日一种可用于临床应用的单克隆抗体，其识别在人免疫缺陷病毒（HIV）的包被膜上具有约1.2×10 5道尔顿（gp120）分子量的糖蛋白抗原的V3-PND区的保守区，具有中和各种各样的HIV变体或其片段的能力，并且提供了由其衍生的嵌合和人源化抗体。通过使用多种具有含有HIV gp120的PND内的高度保守的GPGR序列的PND-Tip区的肽，可以制备对许多HIV变体具有中和活性的单克隆抗体。通过将编码所述区域的所述单克隆抗体或互补决定区（CDR）的可变区的基因片段移植到人抗体基因，嵌合抗体或具有抗HIV中和活性的重构抗体，其对临床应用有效可以获得。

7. 发明授权

US5760185A Anti-feline herpes virus-1 recombinant antibody and gene fragment coding for said antibody 失效
标题翻译：抗猫疱疹病毒-1重组抗体和编码所述抗体的基因片段
公开(公告)号：US5760185A
公开(公告)日：1998-06-02
申请号：US436463
申请日：1995-05-26
申请人： Kazuhiko Kimachi , Hiroaki Maeda , Kiyoto Nishiyama , Sachio Tokiyoshi
发明人： Kazuhiko Kimachi , Hiroaki Maeda , Kiyoto Nishiyama , Sachio Tokiyoshi
IPC分类号： A61K39/395 , A61K38/00 , A61P31/12 , C07K14/00 , C07K14/03 , C07K16/00 , C07K16/08 , C12N15/02 , C12N15/09 , C12N15/13 , C12P21/08 , C12R1/91
CPC分类号： C07K16/085 , A61K38/00 , C07K2317/24 , C07K2319/00 , Y10S424/80 , Y10S424/801 , Y10S424/809
摘要： An anti-FHV-1 recombinant antibody efficacious for treatment, prevention and diagnosis of feline herpes virus-1 (FHV-1) and a gene fragment useful for preparing the same are provided. A cell producing a mouse monoclonal antibody having an excellent neutralizing activity against FHV-1 was constructed, and a gene fragment coding for V region of said antibody responsible for the specific binding activity against FHV-1 was obtained. Using this gene fragment and a gene fragment coding for the constant region of a feline antibody, a chimeric anti-FHV-1 recombinant antibody is obtained.
摘要翻译： PCT No.PCT / JP93 / 01724 Sec。 371日期：1995年5月26日 102（e）日期1995年5月26日PCT提交1993年11月25日PCT公布。公开号WO94 / 12661 日期1994年9月6日提供了一种用于治疗，预防和诊断猫疱疹病毒-1（FHV-1）的抗FHV-1重组抗体和用于制备其的基因片段。构建了对FHV-1具有优异的中和活性的产生小鼠单克隆抗体的细胞，获得了编码针对FHV-1的特异性结合活性的所述抗体的V区的基因片段。使用该基因片段和编码猫抗体恒定区的基因片段，获得嵌合抗FHV-1重组抗体。

8. 发明授权

US5683891A Method for producing exogenous protein 失效
标题翻译：生产外源蛋白的方法
公开(公告)号：US5683891A
公开(公告)日：1997-11-04
申请号：US436462
申请日：1995-05-26
申请人： Kiyoto Nishiyama , Yuji Ishikawa , Kazuhiko Kimachi , Hiroaki Maeda , Sachio Tokiyoshi
发明人： Kiyoto Nishiyama , Yuji Ishikawa , Kazuhiko Kimachi , Hiroaki Maeda , Sachio Tokiyoshi
IPC分类号： C07K16/00 , C12N15/85 , C12P21/08 , C07K16/44 , C12N15/06 , C12N15/13
CPC分类号： C12N15/85 , C07K16/00 , C12N2840/20 , C12N2840/44
摘要： A novel method for production of an exogenous protein is provided, the method being suitable for expression of an exogenous protein, especially a recombinant antibody etc., in an eucaryotic cell by utilizing a genetic recombination technique. That is, a novel method for production of an exogenous protein is provided which allows for culture of a cell in which an exogenous gene is introduced in a serum-free medium and an efficient production of an exogenous protein, by using, as a host cell for expression, a fused cell which is prepared by fusing a mouse myeloma and a lymphatic cell and which can be cultured in a serum-free medium.
摘要翻译： PCT No.PCT / JP95 / 01723 Sec。 371日期：1995年5月26日 102（e）日期1995年5月26日PCT提交1993年11月25日PCT公布。公开号WO94 / 12658 日期1994年6月9日提供了一种生产外源蛋白质的新方法，该方法适用于通过利用遗传重组技术在真核细胞中表达外源蛋白，特别是重组抗体等。也就是说，提供了一种用于生产外源蛋白质的新方法，其允许通过使用作为宿主细胞来培养外源基因导入无血清培养基和有效产生外源蛋白质的细胞用于表达融合细胞，其通过融合小鼠骨髓瘤和淋巴细胞并可在无血清培养基中培养而制备。

9. 发明授权

US5593861A Dog-mouse heterohybridoma and gene fragment coding for constant region of canine immunoglobulins 失效
标题翻译：犬小鼠异源杂交瘤和编码犬免疫球蛋白恒定区的基因片段
公开(公告)号：US5593861A
公开(公告)日：1997-01-14
申请号：US306520
申请日：1994-09-15
申请人： Hiroaki Maeda , Yasuyuki Eda , Kazuhiko Kimachi , Yoichi Ono , Sachio Tokiyoshi
发明人： Hiroaki Maeda , Yasuyuki Eda , Kazuhiko Kimachi , Yoichi Ono , Sachio Tokiyoshi
IPC分类号： A61K39/00 , C07K16/46 , C12N5/26 , C12N15/13 , C12N15/00 , C07H21/04 , C12P21/04 , C12P21/06
CPC分类号： C07K16/462 , A61K39/00 , C07K2317/24
摘要： A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin lambda chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin kappa chain; a gene fragment which comprises a DNA sequence coding for the constant region of canine immunoglobulin gamma chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a canine immunoglobulin; a polypeptide of a mouse-dog chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule cording for an amino acid sequence of the mouse-dog chimeric antibody is incorporated; a dog-mouse heterohybridoma which produces canine immunoglobulin; and a process for preparing a canine immunoglobulin gene.
摘要翻译：一种基因片段，其包含编码犬免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 基因片段，其包含编码犬免疫球蛋白κ链恒定区的氨基酸序列的DNA序列; 包含编码犬免疫球蛋白γ链的恒定区的DNA序列的基因片段; 编码小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段的犬免疫球蛋白; 从用细胞表达载体转化的细胞中表达小鼠 - 狗嵌合抗体的多肽，其中并入所述针对小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子; 产生犬免疫球蛋白的狗 - 小鼠异源杂交瘤; 以及制备犬免疫球蛋白基因的方法。

10. 发明申请

US20060024322A1 Novel prophylactics/remedies for immunopathy 审中-公开
标题翻译：免疫疾病的新型预防措施/补救措施
公开(公告)号：US20060024322A1
公开(公告)日：2006-02-02
申请号：US11122058
申请日：2005-05-05
申请人： Takumi Sasaki , Kazuhiko Kimachi , Kenji Soejima , Yumi Kimura , Chikateru Nozaki , Yoshihide Fujiyama
发明人： Takumi Sasaki , Kazuhiko Kimachi , Kenji Soejima , Yumi Kimura , Chikateru Nozaki , Yoshihide Fujiyama
IPC分类号： A61K39/02 , C07K14/31
CPC分类号： C07K14/31 , A61K38/164 , A61K39/085
摘要： A prophylactic/remedy for immunopathy for immunopathy comprising, as an active ingredient, modifications of Staphylococcal enterotoxin B (SEB) with substitution of at least one amino acid residues within the amino acid sequence of natural type SEB, or derivatives thereof, wherein the SEB modifications or derivatives thereof have inhibitory activity on T cell activation wherein they interact with specific Vβ component of T cell receptor (TCR) but are reduced in their immunological responsiveness to SEB without inducing elimination of T cells having specific Vβ component, the elimination being normally induced by natural type SEB or recombinant wild-type SEB.
摘要翻译：一种用于免疫病的免疫病的预防/治疗方法，包括用天然型SEB的氨基酸序列取代至少一个氨基酸残基的葡萄球菌肠毒素B（SEB）或其衍生物作为活性成分的修饰，其中SEB修饰或其衍生物对T细胞活化具有抑制活性，其中它们与T细胞受体（TCR）的特异性Vbeta组分相互作用，但是其对SEB的免疫应答降低，而不诱导具有特异性Vbeta成分的T细胞的消除，通常由天然型SEB或重组野生型SEB。

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