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1. 发明授权

US5968817A DNA encoding serotonin receptors 失效
标题翻译：编码血清素受体的DNA
公开(公告)号：US5968817A
公开(公告)日：1999-10-19
申请号：US31538
申请日：1993-03-15
申请人： J. Gregor Sutcliffe , Mark G. Erlander , Timothy W. Lovenberg
发明人： J. Gregor Sutcliffe , Mark G. Erlander , Timothy W. Lovenberg
IPC分类号： C07K14/705 , C12N15/12
CPC分类号： C07K14/70571
摘要： The present invention describes nucleic acid molecules encoding human serotonin receptors, recombinant serotonin receptor proteins, cultured cells expressing recombinant serotonin receptor proteins, antibodies immunoreactive with serotonin receptor proteins, polypeptide serotonin receptor antagonists, oligonucleotide probes used for detecting nucleic acids which encode a human serotonin receptor, and nonhuman transgenic animals which express recombinant human serotonin receptor. Also disclosed are methods for screening for ligand binding to the described serotonin receptors and for serotonin receptor agonists and antagonists, for detection of serotonin receptors in tissues, and for therapeutic treatments involving the described human serotonin receptors.
摘要翻译：本发明描述了编码人5-羟色胺受体的核酸分子，重组5-羟色胺受体蛋白，表达重组5-羟色胺受体蛋白的培养细胞，与5-羟色胺受体蛋白免疫反应的抗体，多肽血清素受体拮抗剂，用于检测编码人血清素受体的核酸的寡核苷酸探针，以及表达重组人血清素受体的非人类转基因动物。还公开了筛选与所述5-羟色胺受体和5-羟色胺受体激动剂和拮抗剂结合的配体，用于检测组织中的5-羟色胺受体以及涉及所述人血清素受体的治疗性治疗的方法。

2. 发明授权

US06309834B1 Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations 失效
标题翻译：同时鉴别差异表达mRNA的方法和相对浓度的测定
公开(公告)号：US06309834B1
公开(公告)日：2001-10-30
申请号：US09316349
申请日：1999-05-21
申请人： J. Gregor Sutcliffe , Mark G. Erlander
发明人： J. Gregor Sutcliffe , Mark G. Erlander
IPC分类号： C12Q168
CPC分类号： C12N15/1096 , C12Q1/6809 , C12Q1/6853 , C12Q2600/158 , C12Q2545/114 , C12Q2537/143 , C12Q2531/113 , C12Q2525/185 , C12Q2525/173 , C12Q2525/131
摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3′-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3′-primer whose sequence is derived from the vector and a set of 5′-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.
摘要翻译：用于mRNA同种序列特异性鉴定mRNA群体的改进方法允许将组织表达的几乎每个mRNA作为凝胶上的不同条带进行可视化，其强度大致对应于mRNA的浓度。通常，该方法包括使用锚定引物形成cDNA以固定3'端点，在含有噬菌体特异性启动子的载体中从cDNA产生克隆的插入片段用于随后的RNA合成，产生克隆插入物的线性化片段，制备 cRNA，使用一组引物从cRNA转录cDNA，并使用序列衍生自载体的3'-引物进行PCR，以及从用于从cRNA转录cDNA的引物衍生的一组5'-引物。该方法可以鉴定与给药药物或与生理或病理状况相关的mRNA的表达变化。

3. 发明授权

US5807680A Method for simulataneous identification of differentially expresed mRNAS and measurement of relative concentrations 失效
标题翻译：用于模拟鉴别差异表达mRNAs的方法和相对浓度的测量
公开(公告)号：US5807680A
公开(公告)日：1998-09-15
申请号：US544577
申请日：1995-10-17
申请人： J. Gregor Sutcliffe , Mark G. Erlander
发明人： J. Gregor Sutcliffe , Mark G. Erlander
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N15/10 , C12P19/34 , C12P21/02 , C12Q1/68 , C12Q1/70 , C12R1/19 , G01N33/48
CPC分类号： C12N15/1096 , C12Q1/6809 , C12Q1/6853 , C12Q2600/158
摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3'-primer whose sequence is derived from the vector and a set of 5'-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.
摘要翻译：用于mRNA同种序列特异性鉴定mRNA群体的改进方法允许将组织表达的几乎每个mRNA作为凝胶上的不同条带进行可视化，其强度大致对应于mRNA的浓度。通常，该方法包括使用锚定引物形成cDNA以固定3'端点，在含有噬菌体特异性启动子的载体中从cDNA产生克隆的插入片段用于随后的RNA合成，产生克隆插入物的线性化片段，制备 cRNA，使用一组引物从cRNA转录cDNA，并使用序列衍生自载体的3'-引物进行PCR，以及从用于从cRNA转录cDNA的引物衍生的一组5'-引物。该方法可以鉴定与给药药物或生理或病理状况相关的mRNA的表达变化。

4. 发明授权

US06596484B1 Method for simultaneous sequence-specific identification and display of mRNA's 失效
标题翻译：同时序列特异性鉴定和mRNA显示的方法
公开(公告)号：US06596484B1
公开(公告)日：2003-07-22
申请号：US09650229
申请日：2000-08-29
申请人： J. Gregor Sutcliffe , Mark G. Erlander , Karl W. Hasel
发明人： J. Gregor Sutcliffe , Mark G. Erlander , Karl W. Hasel
IPC分类号： C12Q168
CPC分类号： C12Q1/6853 , C12N15/1096 , C12Q1/6809 , C12Q2535/138 , C12Q2525/185 , C12Q2525/173 , C12Q2525/131
摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3′-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3′-primer whose sequence is derived from the vector and a set of 5′-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.
摘要翻译：用于mRNA同种序列特异性鉴定mRNA群体的改进方法允许将组织表达的几乎每个mRNA作为凝胶上的不同条带进行可视化，其强度大致对应于mRNA的浓度。通常，该方法包括使用锚定引物形成cDNA以固定3'端点，在含有噬菌体特异性启动子的载体中从cDNA产生克隆的插入片段用于随后的RNA合成，产生克隆插入物的线性化片段，制备 cRNA，使用一组引物从cRNA转录cDNA，并使用序列衍生自载体的3'-引物进行PCR，以及从用于从cRNA转录cDNA的引物衍生的一组5'-引物。该方法可以鉴定与给药药物或生理或病理状况相关的mRNA的表达变化。

5. 发明授权

US6110680A Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations 失效
公开(公告)号：US6110680A
公开(公告)日：2000-08-29
申请号：US108100
申请日：1998-06-30
申请人： J. Gregor Sutcliffe , Mark G. Erlander , Karl W. Hasel
发明人： J. Gregor Sutcliffe , Mark G. Erlander , Karl W. Hasel
IPC分类号： G01N33/50 , C12N15/09 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70 , G01N33/15 , G01N33/48 , G06F17/30
CPC分类号： C12Q1/6853 , C12N15/1096 , C12Q1/6809
摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3'-primer whose sequence is derived from the vector and a set of 5'-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.

6. 发明授权

US6030784A Method for simultaneous identification of differentially expressed mRNAS and measurement of relative concentrations 失效
标题翻译：同时鉴别差异表达mRNAs的方法和相对浓度的测定
公开(公告)号：US6030784A
公开(公告)日：2000-02-29
申请号：US35190
申请日：1998-03-05
申请人： J. Gregor Sutcliffe , Mark G. Erlander
发明人： J. Gregor Sutcliffe , Mark G. Erlander
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N15/10 , C12P19/34 , C12P21/02 , C12Q1/68 , C12Q1/70 , C12R1/19 , G01N33/48
CPC分类号： C12N15/1096 , C12Q1/6809 , C12Q1/6853 , C12Q2600/158
摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3'-primer whose sequence is derived from the vector and a set of 5'-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.
摘要翻译：用于mRNA同种序列特异性鉴定mRNA群体的改进方法允许将组织表达的几乎每个mRNA作为凝胶上的不同条带进行可视化，其强度大致对应于mRNA的浓度。通常，该方法包括使用锚定引物形成cDNA以固定3'端点，在含有噬菌体特异性启动子的载体中从cDNA产生克隆的插入片段用于随后的RNA合成，产生克隆插入物的线性化片段，制备 cRNA，使用一组引物从cRNA转录cDNA，并使用序列衍生自载体的3'-引物进行PCR，以及从用于从cRNA转录cDNA的引物衍生的一组5'-引物。该方法可以鉴定与给药药物或生理或病理状况相关的mRNA的表达变化。

7. 发明授权

US5459037A Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations 失效
公开(公告)号：US5459037A
公开(公告)日：1995-10-17
申请号：US152482
申请日：1993-11-12
申请人： J. Gregor Sutcliffe , Mark G. Erlander
发明人： J. Gregor Sutcliffe , Mark G. Erlander
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N15/10 , C12P19/34 , C12P21/02 , C12Q1/68 , C12Q1/70 , C12R1/19 , G01N33/48 , C12N15/11
CPC分类号： C12N15/1096 , C12Q1/6809 , C12Q1/6853 , C12Q2600/158
摘要： An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3'-primer whose sequence is derived from the vector and a set of 5'-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.

8. 发明申请

US20100150944A1 METHODS AND COMPOSITIONS FOR DIAGNOSIS AND TREATMENT OF DEPRESSION AND ANXIETY 审中-公开
标题翻译：用于诊断和治疗呕吐和恶心的方法和组合物
公开(公告)号：US20100150944A1
公开(公告)日：2010-06-17
申请号：US12596427
申请日：2008-04-15
申请人： Brian S. Hilbush , Peter B. Hedlund , Floyd E. Bloom , J. Gregor Sutcliffe
发明人： Brian S. Hilbush , Peter B. Hedlund , Floyd E. Bloom , J. Gregor Sutcliffe
IPC分类号： A61K39/395 , A61K31/7052 , A61K31/352 , A61K36/906 , A61K31/519 , A61K31/5513 , A61K49/00 , C12Q1/48 , G01N33/68 , A61P25/24 , A61P25/22
CPC分类号： A61K36/24 , A61K31/7088 , A61K36/9068 , A61K45/06 , A61K2300/00
摘要： The present invention relates to identification of cellular components, genotypes and gene expression profiles associated with mood disorders. In some embodiments, the present invention relates to the correlation between ribosomal protein S6 (RPS6) and depression and/or anxiety. Embodiments of the present invention further relate to regulation of the activity of RPS6, e.g., by p90 Ribosomal S6 protein kinase. Embodiments of the present invention provide methods and compositions for, e.g., diagnosing, treating, and monitoring depression and/or anxiety, or risk thereof, and for selecting, monitoring, and tailoring treatments for depression and/or anxiety.
摘要翻译：本发明涉及鉴别与情绪障碍相关的细胞成分，基因型和基因表达谱。在一些实施方案中，本发明涉及核糖体蛋白S6（RPS6）与抑郁和/或焦虑之间的相关性。本发明的实施方案还涉及调节RPS6的活性，例如通过p90核糖体S6蛋白激酶的调节。本发明的实施方案提供了用于例如诊断，治疗和监测抑郁症和/或焦虑或其风险以及用于选择，监测和定制抑郁症和/或焦虑治疗的方法和组合物。

9. 发明申请

US20100120787A1 MODIFICATION OF AMYLOID-BETA LOAD IN NON-BRAIN TISSUE 审中-公开
标题翻译：非脑组织中AMYLOID-BETA载体的修饰
公开(公告)号：US20100120787A1
公开(公告)日：2010-05-13
申请号：US12618656
申请日：2009-11-13
申请人： J. Gregor Sutcliffe , Floyd E. Bloom , Brian S. Hilbush
发明人： J. Gregor Sutcliffe , Floyd E. Bloom , Brian S. Hilbush
IPC分类号： A61K31/496 , C12Q1/68 , A61P25/28 , A61K31/506 , A61K31/519
CPC分类号： A61K31/506 , A61K9/0053 , A61K31/00 , A61K31/496 , A61K45/06 , C12N15/113 , C12N2310/14 , G01N33/6896 , G01N2333/4709 , G01N2800/2821
摘要： The present invention relates to methods and compositions for modulating levels of amyloid-β peptide (Aβ) exhibited by non-neuronal (i.e., peripheral) cells, fluids, or tissues. The invention also relates to modulation of Aβ levels via selective modulation (e.g., inhibition) of γ-secretase activity. The invention also relates to methods of preventing, treating or ameliorating the symptoms of a disorder, including but not limited to an Aβ-related disorder, by administering a compound that result in the modulation of γ-secretase in a non-neuronal tissue, either directly or indirectly to prevent, treat or ameliorate the symptoms of a brain Aβ disorder, such as Alzheimer's disease.
摘要翻译：本发明涉及调节淀粉样蛋白水平的方法和组合物，由非神经元（即外周）细胞，流体或组织表现的肽（A＆bgr）。本发明还涉及A＆Bgr的调制。通过γ-分泌酶活性的选择性调节（例如抑制）。本发明还涉及通过在非神经元组织中施用导致γ-分泌酶调节的化合物来预防，治疗或改善病症症状，包括但不限于有关疾病的方法，直接或间接地预防，治疗或改善大脑A＆BGR的症状; 障碍，如阿尔茨海默病。

10. 发明授权

US5416017A Cholera toxin gene regulated by tissue-specific promoters 失效
标题翻译：由组织特异性启动子调节的霍乱毒素基因
公开(公告)号：US5416017A
公开(公告)日：1995-05-16
申请号：US37013
申请日：1993-03-25
申请人： Frank H. Burton , J. Gregor Sutcliffe
发明人： Frank H. Burton , J. Gregor Sutcliffe
IPC分类号： A01K67/027 , C07K14/28 , C12N15/31 , C12N15/85 , C12N5/10 , C12N15/11
CPC分类号： C07K14/28 , A01K67/0275 , C12N15/85 , A01K2217/05 , C12N2830/008 , C12N2830/85
摘要： The present invention contemplates a method of physiologic engineering by genetically altering second messenger levels in cells. This method allows the hyperactivation or inhibition of cell function within cells, tissues and animals by introducing a foreign gene that alters a second messenger system. The use of physiologically engineered animals as systems for determining the effectiveness of therapeutic compositions is also contemplated.
摘要翻译：本发明考虑了通过遗传改变细胞中第二信使水平的生理工程方法。该方法允许通过引入改变第二信使系统的外源基因来激活或抑制细胞，组织和动物中的细胞功能。还考虑使用生理工程化的动物作为确定治疗组合物的有效性的系统。

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