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1. 发明授权

US08802170B2 Method of manufacturing liquid koji 有权
标题翻译：制造液体曲的方法
公开(公告)号：US08802170B2
公开(公告)日：2014-08-12
申请号：US11547809
申请日：2005-03-24
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： A23L1/20
CPC分类号： C12G3/02 , C12G3/12
摘要： An object of the present invention is to provide a liquid koji to be used in the production of a fermented food or drink, particularly a liquid koji having glucoamylase and acid-stable α-amylase with high enzymatic activities, which can be used in the brewing of shochu. The present invention provides a method of manufacturing a liquid koji to be used in production of a fermented food or drink, comprising: cultivating a koji mold in a liquid medium that contains, as a raw material, a substance selected from the group consisting of a cereal having a surface covered with a husk; a cereal having a surface from which only a husk (e.g., a chaff) is removed; an unprocessed bean or tuber having a surface covered with a hull; and amaranthus and/or quinoa. According to the present invention, both enzymes of glucoamylase and acid-stable α-amylase are simultaneously produced with high yield in a balanced manner to allow the production of a liquid koji having enzymatic activities required for, for example, the brewing of shochu. By using the liquid koji, fermented foods and drinks such as shochu can be efficiently produced.
摘要翻译：本发明的目的是提供一种用于生产发酵食品或饮料的液体曲，特别是具有高酶活性的葡糖淀粉酶和酸稳定性α-淀粉酶的液体曲，可用于酿造的烧酒。本发明提供一种制造用于生产发酵食品或饮料的液体曲的方法，包括：在液体培养基中培养曲霉，所述液体培养基含有选自以下的物质作为原料：谷物表面覆盖着果壳; 具有仅除去壳（例如谷壳）的表面的谷物; 未加工的豆或块茎，其表面覆盖有船体; 和苋菜和/或藜麦。根据本发明，以均衡的方式同时产生葡糖淀粉酶和酸稳定性α-淀粉酶的两种酶，以制备具有例如烧制啤酒所需的酶活性的液体曲。通过使用液体曲，可以有效地生产发酵食品和饮料如烧酒。

2. 发明申请

US20090233332A1 METHOD OF PRODUCING RECOMBINANT PROTEIN 有权
标题翻译：生产重组蛋白的方法
公开(公告)号：US20090233332A1
公开(公告)日：2009-09-17
申请号：US12090022
申请日：2006-09-26
申请人： Hiroshi Shoji , Toshikazu Sugimoto
发明人： Hiroshi Shoji , Toshikazu Sugimoto
IPC分类号： C12P21/06
CPC分类号： C12N1/14 , C12N15/80 , C12P21/02
摘要： An object of the present invention is to provide a method of mass-producing recombinant protein by the liquid culture method with koji molds as host. According to the present invention, it is provided a method of producing recombinant protein by using the recombinant koji molds which are obtained by transforming koji molds as host comprising: culturing the recombinant koji molds in a liquid medium which contains as culture raw material at least one selected from the group consisting of the cereal of which surface is entirely or partly covered with at least husks, the bean and/or the tuber of which surface is covered with hulls and the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing; and collecting the recombinant protein from the culture product.
摘要翻译：本发明的目的是提供一种通过液体培养法以曲霉为主体大量生产重组蛋白的方法。根据本发明，提供了通过使用通过转化曲霉作为宿主获得的重组曲霉菌来制备重组蛋白的方法，包括：将重组曲霉菌在含有作为培养原料的液体培养基中培养至少一种选自由表面完全或部分地被至少外壳覆盖的谷物组成的组，其中表面被壳体覆盖的豆和/或块茎，苋菜和/或藜麦没有预处理如研磨或破碎并从培养产物中收集重组蛋白。

3. 发明申请

US20100120119A1 METHOD OF PRODUCING LIQUID KOJI 审中-公开
标题翻译：生产液体KOJI的方法
公开(公告)号：US20100120119A1
公开(公告)日：2010-05-13
申请号：US11995942
申请日：2006-07-20
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： C12N9/30
CPC分类号： C12N1/14 , A23L7/104 , A23L27/50 , C12F3/06 , C12N9/242 , C12N9/2434 , C12N9/58
摘要： An object of the present invention is to provide a method of producing liquid koji having high enzymatic activity by optimizing composition of liquid medium for the liquid koji. In particular, the present invention provides a method of producing liquid koji having enhanced enzymatic activity, the method comprising culturing white koji molds and/or black koji molds in liquid medium containing a nitrogen source by using the cereal of which surface is entirely or partly covered with husks as culture raw material.
摘要翻译：本发明的目的是提供通过优化用于液体曲的液体介质的组成来生产具有高酶活性的液体曲的方法。特别地，本发明提供了具有增强的酶活性的液体曲的生产方法，该方法包括通过使用表面完全或部分覆盖的谷物来培养含有氮源的液体培养基中的白曲霉和/或黑曲霉以壳为文化原料。

4. 发明申请

US20070207238A1 Method of Manufcturing Liquid Koji 有权
标题翻译：制作液体Ko子的方法
公开(公告)号：US20070207238A1
公开(公告)日：2007-09-06
申请号：US11547809
申请日：2005-03-24
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： A23L1/20
CPC分类号： C12G3/02 , C12G3/12
摘要： An object of the present invention is to provide a liquid koji to be used in the production of a fermented food or drink, particularly a liquid koji having glucoamylase and acid-stable α-amylase with high enzymatic activities, which can be used in the brewing of shochu. The present invention provides a method of manufacturing a liquid koji to be used in production of a fermented food or drink, comprising: cultivating a koji mold in a liquid medium that contains, as a raw material, a substance selected from the group consisting of a cereal having a surface covered with a husk; a cereal having a surface from which only a husk (e.g., a chaff) is removed; an unprocessed bean or tuber having a surface covered with a hull; and amaranthus and/or quinoa. According to the present invention, both enzymes of glucoamylase and acid-stable α-amylase are simultaneously produced with high yield in a balanced manner to allow the production of a liquid koji having enzymatic activities required for, for example, the brewing of shochu. By using the liquid koji, fermented foods and drinks such as shochu can be efficiently produced.
摘要翻译：本发明的目的是提供一种用于生产发酵食品或饮料的液体曲，特别是具有高酶活性的具有葡糖淀粉酶和酸稳定的α-淀粉酶的液体曲，可用于酿造中的烧酒。本发明提供一种制造用于生产发酵食品或饮料的液体曲的方法，包括：在液体培养基中培养曲霉，所述液体培养基含有选自以下的物质作为原料：谷物表面覆盖着果壳; 具有仅除去壳（例如谷壳）的表面的谷物; 未加工的豆或块茎，其表面覆盖有船体; 和苋菜和/或藜麦。根据本发明，以均衡的方式同时以高产率生产葡糖淀粉酶和酸稳定性α-淀粉酶的两种酶，以制备具有例如烧制啤酒所需的酶活性的液体曲。通过使用液体曲，可以有效地生产发酵食品和饮料如烧酒。

5. 发明授权

US08124374B2 Method of producing recombinant protein 有权
标题翻译：重组蛋白的制备方法
公开(公告)号：US08124374B2
公开(公告)日：2012-02-28
申请号：US12090022
申请日：2006-09-26
申请人： Hiroshi Shoji , Toshikazu Sugimoto
发明人： Hiroshi Shoji , Toshikazu Sugimoto
IPC分类号： C12P21/06 , C12P21/04 , C12N15/74
CPC分类号： C12N1/14 , C12N15/80 , C12P21/02
摘要： An object of the present invention is to provide a method of mass-producing recombinant protein by the liquid culture method with koji molds as host. According to the present invention, it is provided a method of producing recombinant protein by using the recombinant koji molds which are obtained by transforming koji molds as host comprising: culturing the recombinant koji molds in a liquid medium which contains as culture raw material at least one selected from the group consisting of the cereal of which surface is entirely or partly covered with at least husks, the bean and/or the tuber of which surface is covered with hulls and the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing; and collecting the recombinant protein from the culture product.
摘要翻译：本发明的目的是提供一种通过液体培养法以曲霉为主体大量生产重组蛋白的方法。根据本发明，提供了通过使用通过转化曲霉作为宿主获得的重组曲霉菌来制备重组蛋白的方法，包括：将重组曲霉菌在含有作为培养原料的液体培养基中培养至少一种选自由表面完全或部分地被至少外壳覆盖的谷物组成的组，其中表面被壳体覆盖的豆和/或块茎，苋菜和/或藜麦没有预处理如研磨或破碎并从培养产物中收集重组蛋白。

6. 发明授权

US07998715B2 Method of producing liquid koji having enhanced plant fiber degeneration enzyme, liquid koji obtained by the method and use thereof 有权
标题翻译：具有增强的植物纤维变性酶的液体曲的制备方法及其使用获得的液体曲
公开(公告)号：US07998715B2
公开(公告)日：2011-08-16
申请号：US12067423
申请日：2006-09-05
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： C12P1/02 , C12N1/14 , C12N9/14 , C12N9/30 , C12N9/34
CPC分类号： C12N1/14 , A23L7/104 , A23L27/50 , C12G3/02 , C12P7/04 , Y02E50/17
摘要： An object of the present invention is to provide a method of producing liquid koji having enhanced activity of a plant fiber degradation enzyme using liquid medium without using an expensive plant fiber degradation enzyme preparation and a recombinant bacterium and methods of producing liquid koji dry product and industrial alcohol (ethanol) using the liquid koji. According to the present invention, there is provided a method of producing liquid koji having enhanced activity of a plant fiber degradation enzyme by culturing koji molds with at least one liquid medium selected from the group consisting of a liquid medium containing as culture raw material the cereal of which surface is entirely or partly covered with at least husks, a liquid medium containing as culture raw material the bean and/or the tuber of which surface is covered with hulls, and a liquid medium containing as culture raw material the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing, wherein amount of the culture raw material to be used in the liquid medium is controlled to generate and accumulate simultaneously at least glucoamylase, acid-stable α-amylase and a plant fiber degradation enzyme in the koji mold culture product. There are also provided a method of producing a dry product of liquid koji with liquid medium, characterized by drying the liquid koji obtained by the above-mentioned method, and a method of producing ethanol by a fermentation method using the liquid koji.
摘要翻译：本发明的目的是提供一种使用液体培养基而不使用昂贵的植物纤维降解酶制剂和重组细菌的植物纤维降解酶的活性增加的液体曲的制造方法和制造液体曲柄干燥产品和工业的方法酒精（乙醇）使用液体曲。根据本发明，提供了一种制备具有增强植物纤维降解酶活性的液体曲的方法，该方法通过用至少一种液体培养基培养曲霉，所述液体培养基选自含有作为培养原料的谷物的液体培养基其表面完全或部分地被至少覆盖壳，包含作为培养原料的豆类和/或其表面被壳体覆盖的块茎的液体培养基，以及含有作为培养原料的苋菜和/或不经预处理的藜麦如研磨或粉碎，其中控制在液体介质中使用的培养原料的量至少同时产生并积累至少葡糖淀粉酶，酸稳定的α-淀粉酶和植物纤维降解酶曲曲霉菌产品。还提供了一种用液体培养基制备液体曲的干燥产物的方法，其特征在于干燥通过上述方法获得的液体曲，以及通过使用液体曲的发酵方法生产乙醇的方法。

7. 发明申请

US20090022848A1 METHOD OF PRODUCING LIQUID KOJI HAVING ENHANCED PLANT FIBER DEGRADATION ENZYME, LIQUID KOJI OBTAINED BY THE METHOD AND USE THEREOF 有权
标题翻译：生产具有增强植物纤维降解酶的液体醇的方法和方法获得的液体KOJO
公开(公告)号：US20090022848A1
公开(公告)日：2009-01-22
申请号：US12067423
申请日：2006-09-05
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： C12G3/00 , A23L1/28 , C12P21/00 , C12N1/14 , C12N9/00 , C12N9/30 , C07C31/08
CPC分类号： C12N1/14 , A23L7/104 , A23L27/50 , C12G3/02 , C12P7/04 , Y02E50/17
摘要： An object of the present invention is to provide a method of producing liquid koji having enhanced activity of a plant fiber degradation enzyme using liquid medium without using an expensive plant fiber degradation enzyme preparation and a recombinant bacterium and methods of producing liquid koji dry product and industrial alcohol (ethanol) using the liquid koji. According to the present invention, there is provided a method of producing liquid koji having enhanced activity of a plant fiber degradation enzyme by culturing koji molds with at least one liquid medium selected from the group consisting of a liquid medium containing as culture raw material the cereal of which surface is entirely or partly covered with at least husks, a liquid medium containing as culture raw material the bean and/or the tuber of which surface is covered with hulls, and a liquid medium containing as culture raw material the amaranthus and/or the quinoa without pre-treatment such as grinding or crushing, wherein amount of the culture raw material to be used in the liquid medium is controlled to generate and accumulate simultaneously at least glucoamylase, acid-stable α-amylase and a plant fiber degradation enzyme in the koji mold culture product. There are also provided a method of producing a dry product of liquid koji with liquid medium, characterized by drying the liquid koji obtained by the above-mentioned method, and a method of producing ethanol by a fermentation method using the liquid koji.
摘要翻译：本发明的目的是提供一种使用液体培养基而不使用昂贵的植物纤维降解酶制剂和重组细菌的植物纤维降解酶的活性增加的液体曲的制造方法和制造液体曲柄干燥产品和工业的方法酒精（乙醇）使用液体曲。根据本发明，提供了一种制备具有增强植物纤维降解酶活性的液体曲的方法，该方法通过用至少一种液体培养基培养曲霉，所述液体培养基选自含有作为培养原料的谷物的液体培养基其表面完全或部分地被至少覆盖壳，包含作为培养原料的豆类和/或其表面被壳体覆盖的块茎的液体培养基，以及含有作为培养原料的苋菜和/或没有预处理的藜麦如研磨或粉碎，其中控制在液体介质中使用的培养原料的量至少同时产生并积累至少葡糖淀粉酶，酸稳定的α-淀粉酶和植物纤维降解酶曲曲霉菌产品。还提供了一种用液体培养基制备液体曲的干燥产物的方法，其特征在于干燥通过上述方法获得的液体曲，以及通过使用液体曲的发酵方法生产乙醇的方法。

8. 发明授权

US08715979B2 Method of producing filamentous fungus culture product 失效
标题翻译：丝状真菌培养产物的生产方法
公开(公告)号：US08715979B2
公开(公告)日：2014-05-06
申请号：US12089067
申请日：2006-09-05
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： C12P1/02
CPC分类号： C12N1/14 , C12N9/242 , C12N9/2437 , C12N9/2445 , C12N9/58 , C12N9/62 , C12Y302/01021
摘要： It is an object of the present invention to provide a method of adjusting productivity of enzymes, in particular, amylolytic enzymes, plant fiber degradation enzymes and proteolytic enzymes in a filamentous fungus culture product, by controlling releasing rate of nutrients from the culture raw material into the culture system when a filamentous fungus culture product is produced by culturing filamentous fungi in liquid medium containing as the culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa. The present invention provides a method of producing filamentous fungus culture product by using liquid medium containing as culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa comprising, culturing filamentous fungi while releasing rate of nutrients from the culture raw material into the culture system is controlled to adjust productivity of enzymes in the filamentous fungus culture product.
摘要翻译：本发明的目的是提供一种调节丝状真菌培养物中的酶特别是淀粉分解酶，植物纤维降解酶和蛋白水解酶的生产力的方法，通过将培养原料的营养物质的释放速率控制为当通过在含有选自谷物，豆类，块茎，苋菜和藜麦中的至少一种作为培养原料的液体培养基中培养丝状真菌来生产丝状真菌培养产物时的培养体系。本发明提供一种通过使用含有作为培养原料的液体培养基来制造丝状真菌培养物的方法，所述液体培养基选自谷物，豆类，块茎，苋菜和藜麦中的至少一种，其包括：培养丝状真菌，同时从控制培养原料进入培养系统以调节丝状真菌培养产物中酶的生产力。

9. 发明申请

US20090280212A1 METHOD OF PRODUCING FILAMENTOUS FUNGUS CULTURE PRODUCT 失效
标题翻译：生产真菌真菌培养产品的方法
公开(公告)号：US20090280212A1
公开(公告)日：2009-11-12
申请号：US12089067
申请日：2006-09-05
申请人： Toshikazu Sugimoto , Hiroshi Shoji
发明人： Toshikazu Sugimoto , Hiroshi Shoji
IPC分类号： A23J3/34 , C12P1/02 , C12P19/00 , C12P13/04 , C12N9/00 , A23L1/48 , A23L2/00
CPC分类号： C12N1/14 , C12N9/242 , C12N9/2437 , C12N9/2445 , C12N9/58 , C12N9/62 , C12Y302/01021
摘要： It is an object of the present invention to provide a method of adjusting productivity of enzymes, in particular, amylolytic enzymes, plant fiber degradation enzymes and proteolytic enzymes in a filamentous fungus culture product, by controlling releasing rate of nutrients from the culture raw material into the culture system when a filamentous fungus culture product is produced by culturing filamentous fungi in liquid medium containing as the culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa. The present invention provides a method of producing filamentous fungus culture product by using liquid medium containing as culture raw material at least one selected from the group consisting of cereals, beans, tubers, amaranthus and quinoa comprising, culturing filamentous fungi while releasing rate of nutrients from the culture raw material into the culture system is controlled to adjust productivity of enzymes in the filamentous fungus culture product.
摘要翻译：本发明的目的是提供一种调节丝状真菌培养物中的酶特别是淀粉分解酶，植物纤维降解酶和蛋白水解酶的生产力的方法，通过将培养原料的营养物质的释放速率控制为当通过在含有选自谷物，豆类，块茎，苋菜和藜麦中的至少一种作为培养原料的液体培养基中培养丝状真菌来生产丝状真菌培养产物时的培养体系。本发明提供一种通过使用含有作为培养原料的液体培养基来制造丝状真菌培养物的方法，所述液体培养基选自谷物，豆类，块茎，苋菜和藜麦中的至少一种，其包括：培养丝状真菌，同时从控制培养原料进入培养系统以调节丝状真菌培养产物中酶的生产力。

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