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    • 26. 发明授权
    • Scanning observation apparatus
    • 扫描观察装置
    • US09513223B2
    • 2016-12-06
    • US14829983
    • 2015-08-19
    • OLYMPUS CORPORATION
    • Hiroya Fukuyama
    • G01J1/58G01T1/10G01N21/64G02B21/06G02B21/36
    • G01N21/6456G01N2201/10G01N2201/121G02B21/06G02B21/365
    • A high-resolution fluorescence image in which an afterimage is suppressed is obtained, even when a fluorescence detection interval is shortened. Provided is a scanning observation apparatus including a scanning unit that spatially scans pulsed excitation light emitted from a light source at prescribed time intervals on a specimen; a fluorescence detecting unit that detects fluorescence generated by exciting a fluorescent substance inside the specimen with the excitation light scanned by the scanning unit, in synchronization with the emission of the excitation light; and a fluorescence correcting unit that subtracts, from a fluorescence intensity detected by the fluorescence detecting unit, an afterimage fluorescence component calculated on the basis of time-sequential fluorescence detected by the fluorescence detecting unit prior thereto, at each scanning position, to correct the fluorescence intensity at the scanning position.
    • 即使在荧光检测间隔缩短的情况下也能够得到抑制残像的高分辨率的荧光图像。 本发明提供一种扫描观察装置,其具备:扫描单元,其以规定的时间间隔在样本上空间扫描从光源发出的脉冲激发光; 荧光检测单元,其与通过所述激发光的发射同步地检测利用所述扫描单元扫描的激发光来激发所述样本内的荧光物质而产生的荧光; 以及荧光校正单元,从荧光检测单元检测出的荧光强度,在每个扫描位置根据荧光检测单元检测到的时间序列荧光计算的余像荧光成分,从而校正荧光 在扫描位置的强度。
    • 27. 发明申请
    • METHOD AND SYSTEM FOR DETERMINING ULTRAVIOLET FLUENCE RECEIVED BY A FLUID
    • 用于确定流体接收的超紫外线流体的方法和系统
    • US20160258811A1
    • 2016-09-08
    • US15036962
    • 2014-11-12
    • TROJAN TECHNOLOGIES
    • Mark Adrian KustermansMichael SasgesAnkit PatrasTrisevgeni Trissa Kantzas
    • G01J1/58A61L2/00G01N33/483A61L2/10G01N33/49G01N33/15
    • G01J1/58A61L2/0005A61L2/0047A61L2/10A61L2202/11C02F1/008C02F1/32G01J1/429G01N21/64G01N33/15G01N33/4833G01N33/49G01N2201/13
    • There is described a method of determining the UV fluence received by a fluid. The method comprises the steps of: (a) irradiating the fluid at an unknown UV fluence; (b) measuring the fluorescence of a test sample of the fluid after irradiation in Step (a) to produce a test signal proportional to the concentration of a prescribed fluorescent composition of matter comprised in the test sample; and (c) determining the value of the unknown UV fluence by comparing the test signal to a calibration curve of a control signal proportional to concentration of the prescribed fluorescent composition of matter in the fluid as a function of applied UV fluence. There is also described a system for determining the UV fluence received by a fluid being treated in UV fluid treatment system comprising at least one UV source. The system comprises: (a) a radiation-transparent vessel for receiving a test sample of the fluid after irradiation of the fluid at an unknown UV fluence; (b) a fluorometer for measuring the fluorescence of the test sample received in the radiation-transparent vessel to produce a test signal proportional to the concentration of a prescribed fluorescent composition of matter comprised in the test sample; and (c) a controller configured to determine the value of the unknown UV fluence by comparing the test signal to a calibration curve of a control signal proportional to concentration of the prescribed fluorescent composition of matter in the fluid as a function of applied UV fluence.
    • 描述了确定流体接收的UV注量的方法。 该方法包括以下步骤:(a)以未知的UV注量照射流体; (b)在步骤(a)中测量照射后的流体的测试样品的荧光,以产生与测试样品中包含的物质的规定的荧光组合物的浓度成比例的测试信号; 并且(c)通过将测试信号与作为所施加的UV注量的函数的与流体中规定的荧光成分的浓度成比例的控制信号的校准曲线进行比较来确定未知UV注量的值。 还描述了一种用于确定由包含至少一个UV源的UV流体处理系统中被处理的流体接收的UV注量的系统。 该系统包括:(a)辐射透明容器,用于在未知的紫外线注量流量的流体照射后接收流体的测试样品; (b)荧光计,用于测量接受在辐射透明容器中的测试样品的荧光,以产生与测试样品中包含的物质的规定荧光组合物的浓度成比例的测试信号; 以及(c)控制器,被配置为通过将测试信号与作为所施加的UV注量的函数的流体中规定的荧光成分的浓度成比例的控制信号的校准曲线进行比较来确定未知的UV注量的值。