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51. 发明授权

US09012203B2 Microfluidic device, system, and method for controlled encapsulation of particles or particle clusters 有权
标题翻译：微流体装置，系统和用于颗粒或颗粒簇的可控包封的方法
公开(公告)号：US09012203B2
公开(公告)日：2015-04-21
申请号：US12465112
申请日：2009-05-13
申请人： Florence Rivera , Jean Berthier , Sophie Le Vot
发明人： Florence Rivera , Jean Berthier , Sophie Le Vot
IPC分类号： C12M3/00 , C12N11/04
CPC分类号： C12N11/04
摘要： A microfluidic device for controlled encapsulation of particles of sub-millimetric dimensions, or clusters of such particles, the device comprising: a first duct for delivering a first liquid phase containing particles for encapsulating in suspension; a second duct for conveying a flow of a second liquid phase that is immiscible with said first liquid phase; the first duct opening out into the second duct and forming a fluidic junction therewith; at least one microfluidic duct for discharging the first liquid phase flowing in said first duct and provided with a mouth located upstream from said junction and liable to be obstructed, at least in part, by a particle in suspension, thereby causing pressure to rise in the first duct. The invention also provides a microfluidic system including such a device, and a method of encapsulation based on using such a device.
摘要翻译：一种微流体装置，其用于控制密封亚毫米级的颗粒或这种颗粒的簇，所述装置包括：用于输送含有颗粒的第一液相以封装在悬浮液中的第一管道; 用于输送与所述第一液相不混溶的第二液相的流的第二管道; 第一管道打开进入第二管道并与其形成流体连接点; 至少一个微流体管道，用于排出在所述第一管道中流动的第一液相，并且设置有位于所述接合部上游的口部，并且至少部分地由悬浮物中的颗粒阻塞，从而在第一管。本发明还提供了一种包括这种装置的微流体系统，以及基于使用这种装置的封装方法。

52. 发明申请

US20150099289A1 CARBONIC ANHYDRASE XEROGEL PARTICLES 审中-公开
标题翻译：碳纳米颗粒XEROGEL颗粒
公开(公告)号：US20150099289A1
公开(公告)日：2015-04-09
申请号：US14510807
申请日：2014-10-09
申请人： Akermin, Inc.
发明人： Tracy L. Bucholz , Aleksey Zaks , Dawn Powell , Luke Weber , Matthew Hulvey , John Reardon , Brett Rambo
IPC分类号： B01D19/00 , C12N11/04
CPC分类号： C12N11/04
摘要： The present invention generally relates to carbonic anhydrase xerogel particles comprising a polysilicate-polysilicone copolymer and carbonic anhydrase. The carbonic anhydrase xerogel particles can be used for carbon dioxide capture.
摘要翻译：本发明一般涉及包含聚硅酸酯 - 聚硅氧烷共聚物和碳酸酐酶的碳酸酐酶干凝胶颗粒。碳酸酐酶干凝胶颗粒可用于二氧化碳捕获。

53. 发明授权

US08951800B2 Primate pluripotent stem cell expansion without feeder cells and in the presence of FGF and matrigel or Engelbreth-Holm-Swarm tumor cell preparation 有权
标题翻译：灵长类多能干细胞扩增无饲养细胞，并存在FGF和基质胶或恩格布氏 - 霍姆群肿瘤细胞制备
公开(公告)号：US08951800B2
公开(公告)日：2015-02-10
申请号：US12710078
申请日：2010-02-22
申请人： Ramkumar Mandalam , Chunhui Xu , Joseph D. Gold , Melissa K. Carpenter
发明人： Ramkumar Mandalam , Chunhui Xu , Joseph D. Gold , Melissa K. Carpenter
IPC分类号： C12N5/02 , C12N15/10 , C12N5/0735 , C12N5/0793 , C12N5/079 , C12N5/074 , C12N5/00 , C12N5/071 , C12N11/04 , C12N5/073 , C12N5/09 , C12N5/0775
CPC分类号： C12N15/1096 , C12N5/0062 , C12N5/0068 , C12N5/0075 , C12N5/0603 , C12N5/0606 , C12N5/0607 , C12N5/0619 , C12N5/0622 , C12N5/0662 , C12N5/0671 , C12N5/0672 , C12N5/0678 , C12N5/068 , C12N5/0692 , C12N5/0693 , C12N5/0696 , C12N11/04 , C12N15/1034 , C12N2500/25 , C12N2500/90 , C12N2500/98 , C12N2500/99 , C12N2501/065 , C12N2501/105 , C12N2501/115 , C12N2501/119 , C12N2501/125 , C12N2501/13 , C12N2501/145 , C12N2501/155 , C12N2501/2306 , C12N2501/235 , C12N2501/237 , C12N2501/26 , C12N2501/998 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2506/02 , C12N2510/00 , C12N2510/04 , C12N2533/50 , C12N2533/90
摘要： This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.
摘要翻译：本公开提供了一种用于培养人多能干细胞的改进系统。传统上，多能干细胞在一层饲养细胞（如小鼠胚胎成纤维细胞）上培养以防止它们分化。在这里描述的系统中，饲养细胞的作用由添加到培养环境中的组分代替，其支持快速增殖而不分化。有效的特征是细胞的合适的支持结构，以及可以在不被另一种细胞类型预处理的情况下可以新鲜添加到培养物中的有效培养基。在根据本发明的新鲜培养基中培养人胚胎干细胞导致细胞惊人地快速扩张，同时保持分化成代表所有三个胚胎胚层的细胞的能力。这种新的培养系统允许pPS细胞的大量增殖用于商业生产用于药物筛选和人类治疗的重要产品。

54. 发明授权

US08852904B2 Protein complex having activity catalyzing asymmetric oxidation reaction and process for producing the same 有权
标题翻译：具有催化不对称氧化反应的活性的蛋白质复合物及其制备方法
公开(公告)号：US08852904B2
公开(公告)日：2014-10-07
申请号：US13321520
申请日：2010-05-20
申请人： Hiroyuki Nagaoka
发明人： Hiroyuki Nagaoka
IPC分类号： C12P7/02 , A61L33/00 , C12P7/26 , C12N11/04 , C12P41/00 , C12N9/96 , C12N9/02
CPC分类号： C12P7/02 , C12N9/0004 , C12N9/96 , C12N11/04 , C12P7/26 , C12P41/002 , Y02P20/52 , Y02P20/588
摘要： A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition.
摘要翻译：一种生产交联结晶蛋白复合物的方法，其包括：浓缩源自动物或植物的粗蛋白质的第一步骤; 将蛋白质包封在凝胶中的第二步骤，从而使蛋白质经历空气氧化，然后从凝胶中提取蛋白质复合物; 第三步，使提取的蛋白质复合物进行结晶析出; 以及交联沉淀的蛋白质复合物的第四步骤。或者，通过使用所获得的交联结晶蛋白复合物的干燥（FD）的第五步骤，从而形成粉末。结果，提供了一种在室温下稳定的酶，并且具有催化不对称氧化反应的活性。也就是说，提供了在温和条件下能够进行有效的酶模拟反应的有用材料。

55. 发明授权

US08735117B2 Method for making artificial scaffold having porous three-dimensional body comprising cells 有权
标题翻译：制造包含细胞的多孔三维体的人造骨架的方法
公开(公告)号：US08735117B2
公开(公告)日：2014-05-27
申请号：US12877818
申请日：2010-09-08
申请人： Andrew Darling , Lauren Shor , Wei Sun , Selcuk Guceri
发明人： Andrew Darling , Lauren Shor , Wei Sun , Selcuk Guceri
IPC分类号： C12N11/02 , C12N11/00 , C12N11/14 , C12N11/08 , C12N11/04 , C12N5/00 , C12N5/07 , A61F2/00
CPC分类号： C12N11/02 , A61L27/38 , A61L27/46 , A61L27/48 , A61L27/56 , B29C47/0019 , B29C47/0028 , B29C47/003 , C12N5/0075 , C12N2533/18 , C12N2533/40 , C12N2533/54 , C12N2533/56 , C12N2533/74
摘要： In on aspect, the invention includes a microcarrier bead having a porous three-dimensional core having (a) a polymeric porous three-dimensional body having porosity of about 15 to about 90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns, (b) an outer protective layer and optionally (c) a filler. In another aspect, the invention includes a method of making an artificial scaffold wherein a scaffolding material is extruded into a coolant and thereby creating a porous material having a porosity of between 15-90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns.
摘要翻译：在一方面，本发明包括具有多孔三维芯的微载体珠，其具有（a）具有约15至约90％的孔隙率的聚合多孔三维体，使得至少99％的孔互连并具有直径（b）外保护层和任选的（c）填料。另一方面，本发明包括制备人造支架的方法，其中将脚手架材料挤出成冷却剂，从而产生孔隙率在15-90％之间的多孔材料，使得至少99％的孔相互连接并具有直径至多为200微米。

56. 发明授权

US08697111B2 Osteochondral implant comprising osseous phase and chondral phase 有权
标题翻译：骨软骨植入物包括骨相和软骨相
公开(公告)号：US08697111B2
公开(公告)日：2014-04-15
申请号：US12778256
申请日：2010-05-12
申请人： Arpan Desai , Timothy Sargeant , Atu Agawu , Joshua Stopek
发明人： Arpan Desai , Timothy Sargeant , Atu Agawu , Joshua Stopek
IPC分类号： A61F2/00 , C12N11/02 , C12N11/08 , C12N11/06 , C12N11/04 , G01N33/544 , G01N33/545 , G01N33/549 , C07K17/02 , C07K17/08 , C07K17/06 , C07K17/04
CPC分类号： A61L27/50 , A61B17/00491 , A61B2017/3419 , A61L27/14 , A61L27/54 , A61L27/56
摘要： An osteochondral plug includes a first scaffold and a second scaffold. The first scaffold may be a solid scaffold containing one or more pendant reactive functional groups. The second scaffold capable of reacting with the one or more pendant reactive functional groups of the first scaffold.
摘要翻译：骨软骨塞包括第一支架和第二支架。第一支架可以是含有一个或多个侧链反应性官能团的固体支架。能够与第一支架的一个或多个侧链反应性官能团反应的第二支架。

57. 发明授权

US08691203B2 Method comprising contacting tissue with a cross-linkable polyester prepolymer 有权
标题翻译：方法包括使组织与可交联聚酯预聚物接触
公开(公告)号：US08691203B2
公开(公告)日：2014-04-08
申请号：US13428869
申请日：2012-03-23
申请人： Christopher J. Bettinger , Joost P. Bruggeman , Lino Da Silva Ferreira , Jeffrey M. Karp , Robert S. Langer , Christiaan Nijst , Andreas Zumbuehl , Jason Burdick , Sonia J. Kim
发明人： Christopher J. Bettinger , Joost P. Bruggeman , Lino Da Silva Ferreira , Jeffrey M. Karp , Robert S. Langer , Christiaan Nijst , Andreas Zumbuehl , Jason Burdick , Sonia J. Kim
IPC分类号： A61K31/74 , A61F2/00 , A61K9/52 , A61K9/14 , C12N11/08 , C12N11/04 , C12N5/00 , C12N5/07
CPC分类号： A61L31/06 , A61K35/12 , A61K35/48 , A61K45/06 , A61L27/18 , A61L27/38 , A61L27/58 , A61L31/148 , C08G63/6854 , C08J3/24 , C08J3/246 , C08J2367/00 , C08J2367/07 , C08K5/0025 , C12N11/08 , C08L67/00 , C08L67/07
摘要： The present inventions in various aspects provide elastic biodegradable polymers. In various embodiments, the polymers are formed by the reaction of a multifunctional alcohol or ether and a difunctional or higher order acid to form a pre-polymer, which is cross-linked to form the elastic biodegradable polymer. In preferred embodiments, the cross-linking is performed by functionalization of one or more OR groups on the pre-polymer backbone with vinyl, followed by photopolymerization to form the elastic biodegradable polymer composition or material. Preferably, acrylate is used to add one or more vinyls to the backbone of the pre-polymer to form an acrylated pre-polymer. In various embodiments, acrylated pre-polymers are co-polymerized with one or more acrylated co-polymers.
摘要翻译：本发明在各方面提供弹性可生物降解的聚合物。在各种实施方案中，聚合物通过多官能醇或醚和双官能或更高级酸的反应形成以形成预聚物，其被交联以形成弹性可生物降解的聚合物。在优选的实施方案中，通过用乙烯基官能化预聚物主链上的一个或多个OR基团进行交联，然后进行光聚合以形成弹性可生物降解的聚合物组合物或材料。优选地，使用丙烯酸酯将一种或多种乙烯基加成到预聚物的主链上以形成丙烯酸酯化的预聚物。在各种实施方案中，丙烯酸酯化的预聚物与一种或多种丙烯酸酯化共聚物共聚。

58. 发明授权

US08679809B2 Cell-matrix microspheres, methods for preparation and applications 有权
标题翻译：细胞基质微球，制备方法及应用
公开(公告)号：US08679809B2
公开(公告)日：2014-03-25
申请号：US11750863
申请日：2007-05-18
申请人： Barbara Pui Chan , Godfrey Chi-Fung Chan , Hoi Ling Wong , Pik To Cheung , Song-Eng Kathryn Cheah , Danny Chan
发明人： Barbara Pui Chan , Godfrey Chi-Fung Chan , Hoi Ling Wong , Pik To Cheung , Song-Eng Kathryn Cheah , Danny Chan
IPC分类号： C12N11/04
CPC分类号： C12N5/0602 , A61K35/12 , C12M25/14 , C12N5/0012 , C12N5/0655 , C12N5/0663 , C12N2510/02 , C12N2533/54 , C12N2533/90
摘要： A method has been developed to produce stable cell-matrix microspheres with up to 100% encapsulation efficiency and high cell viability, using matrix or biomaterial systems with poor shape and mechanical stability for applications including cell therapeutics via microinjection or surgical implantation, 3D culture for in vitro expansion without repeated cell splitting using enzymatic digestion or mechanical dissociation and for enhanced production of therapeutic biomolecules, and in vitro modeling for morphogenesis studies. The modified droplet generation method is simple and scalable and enables the production of cell-matrix microspheres when the matrix or biomaterial system used has low concentration, with slow phase transition, with poor shape and mechanical stability.
摘要翻译：已经开发了一种方法来生产具有高达100％包封效率和高细胞活力的稳定的细胞基质微球，使用具有差的形状和机械稳定性的基质或生物材料体系，包括通过显微注射或手术植入的细胞治疗剂，3D培养物使用酶消化或机械解离并且用于增强治疗性生物分子的产生的体外扩增而不重复细胞分裂，以及用于形态发生研究的体外模拟。改进的液滴生成方法简单可伸缩，当使用的基质或生物材料系统具有低浓度，缓慢的相变，形状和机械稳定性差时，能够生产细胞基质微球。

59. 发明授权

US08679807B2 Covalent immobilization of molecules comprising an amino group 有权
标题翻译：包含氨基的分子的共价固定化
公开(公告)号：US08679807B2
公开(公告)日：2014-03-25
申请号：US13812101
申请日：2011-07-27
申请人： Sven Oscarsson , Kristofer Eriksson , Leif Nyholm
发明人： Sven Oscarsson , Kristofer Eriksson , Leif Nyholm
IPC分类号： C12N11/00 , C12N11/06 , C12N11/04
CPC分类号： C07K17/10 , C07K17/14 , C12N11/00 , G01N33/54353
摘要： There is provided a method for covalent immobilization of at least one molecule comprising at least one amino group, said method comprising the sequential steps of: a) providing a surface comprising —SH groups, b) oxidizing the surface comprising —SH groups using redox reactions in the presence of noble metal ions, and c) contacting the surface with at least one molecule comprising at least one amino group to obtain a covalent binding of the at least one molecule to the surface, wherein said at least one amino group is involved in obtaining said covalent bond. The immobilized molecules are immobilized via stable covalent bonds. The method is more versatile since it can be performed as a one step method. All reaction steps are performed in aqueous solution. All steps can be performed at room temperature. The chemicals used are less expensive and less toxic compared to the prior art.
摘要翻译：提供了至少一种包含至少一个氨基的分子共价固定的方法，所述方法包括以下顺序步骤：a）提供包含-SH基团的表面，b）使用氧化还原反应氧化包含-SH基团的表面在贵金属离子的存在下，和c）使所述表面与至少一个包含至少一个氨基的分子接触，以获得所述至少一个分子与所述表面的共价结合，其中所述至少一个氨基参与获得所述共价键。固定化的分子通过稳定的共价键固定。该方法更通用，因为它可以作为一步法进行。所有反应步骤均在水溶液中进行。所有步骤均可在室温下进行。与现有技术相比，使用的化学品便宜且毒性较小。

60. 发明授权

US08535924B2 Granules with reduced dust potential comprising an antifoam agent 有权
标题翻译：具有降低粉尘潜力的颗粒包含消泡剂
公开(公告)号：US08535924B2
公开(公告)日：2013-09-17
申请号：US13288680
申请日：2011-11-03
申请人： Douglas A. Dale , Thomas S. Green
发明人： Douglas A. Dale , Thomas S. Green
IPC分类号： C12N9/98 , C12N11/18 , C12N11/14 , C12N11/02 , C12N11/08 , C12N11/04 , D06M16/00 , C11D7/42 , C11D3/386
CPC分类号： C11D17/0039 , C11D3/38672 , G01N33/57415 , G01N33/57419 , G01N33/57449 , G01N33/6872 , Y10T428/2991 , Y10T428/2998
摘要： A granule with an allergenic component has reduced dust by including antifoam added during the production of the granule. The antifoam may be dispersed throughout the granule or added to one of the components of the granule. The granule with antifoam produces at least 30% less dust than a comparable granule produced according to a process in which no antifoam is added.
摘要翻译：具有过敏成分的颗粒通过在颗粒生产期间加入消泡剂来减少粉尘。消泡剂可以分散在整个颗粒中或加入到颗粒的一种组分中。具有消泡剂的颗粒与根据不加入消泡剂的方法制备的相当的颗粒相比，产生的灰尘少至少30％。

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