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81. 发明申请

US20080138344A1 Identification and Engineering of Antibodies with Variant Fc Regions and Methods of Using Same 有权
标题翻译：具有变异Fc区的抗体的鉴定和工程及其使用方法
公开(公告)号：US20080138344A1
公开(公告)日：2008-06-12
申请号：US12022762
申请日：2008-01-30
申请人： Jeffrey Stavenhagen , Sujata Vijh , Christopher Rankin , Sergey Gorlatov , Ling Huang
发明人： Jeffrey Stavenhagen , Sujata Vijh , Christopher Rankin , Sergey Gorlatov , Ling Huang
IPC分类号： A61K39/395 , C07K16/00 , C07H21/04 , C12N15/00 , C12N5/00 , C12P21/04
CPC分类号： C07K14/70535 , C07K14/36 , C07K14/405 , C07K16/005 , C07K16/283 , C07K16/2887 , C07K16/32 , C07K16/44 , C07K2317/24 , C07K2317/52 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/76 , C07K2317/92 , C07K2319/70 , G01N33/6857 , G01N2333/70535
摘要： The present invention relates to molecules, particularly polypeptides, more particularly immunoglobulins (e.g., antibodies), comprising a variant Fc region, wherein said variant Fc region comprises at least one amino acid modification relative to a wild-type Fc region, which variant Fc region binds FcγRIIIA and/or FcγRIIA with a greater affinity, relative to a comparable molecule comprising the wild-type Fc region. The molecules of the invention are particularly useful in preventing, treating, or ameliorating one or more symptoms associated with a disease, disorder, or infection. The molecules of the invention are particularly useful for the treatment or prevention of a disease or disorder where an enhanced efficacy of effector cell function (e.g., ADCC) mediated by FcγR is desired, e.g., cancer, infectious disease, and in enhancing the therapeutic efficacy of therapeutic antibodies the effect of which is mediated by ADCC.
摘要翻译：本发明涉及包括变体Fc区的分子，特别是多肽，更特别是免疫球蛋白（例如，抗体），其中所述变体Fc区相对于野生型Fc区包含至少一个氨基酸修饰，所述变体Fc区相对于包含野生型Fc区的可比较分子，以更大的亲和力结合FcγRIIIA和/或FcγRIIA。本发明的分子特别可用于预防，治疗或改善与疾病，病症或感染相关的一种或多种症状。本发明的分子特别可用于治疗或预防需要通过FcγAMR介导的效应细胞功能增强的功效（例如ADCC）的疾病或病症，例如癌症，感染性疾病和增强治疗功效的治疗性抗体，其作用由ADCC介导。

82. 发明申请

US20080044417A1 Humanized Fc.gamma.RIIB-Specific Antibodies and Methods of Use Thereof 有权
标题翻译：人源化Fc.gamma.RIIB特异性抗体及其使用方法
公开(公告)号：US20080044417A1
公开(公告)日：2008-02-21
申请号：US11754015
申请日：2007-05-25
申请人： Leslie Johnson , Ling Huang
发明人： Leslie Johnson , Ling Huang
IPC分类号： A61K39/395 , A61P43/00 , C07H21/04 , C07K16/18 , C12N15/00 , C12N5/06 , C12P21/04 , G01N33/564
CPC分类号： A61K39/39 , A61K2039/505 , A61K2039/55516 , C07K16/283 , C07K2317/24 , C07K2317/34 , C07K2317/52 , G01N33/564 , G01N33/6893 , G01N2800/24
摘要： The present invention relates to humanized FcγRIIB antibodies, fragments, and variants thereof that bind human FcγRIIB with a greater affinity than said antibody binds FcγRIIA. The invention encompasses the use of the humanized antibodies of the invention for the treatment of any disease related to loss of balance of Fc receptor mediated signaling, such as cancer, autoimmune and inflammatory disease. The invention provides methods of enhancing the therapeutic effect of therapeutic antibodies by administering the humanized antibodies of the invention to enhance the effector function of the therapeutic antibodies. The invention also provides methods of enhancing the efficacy of a vaccine composition by administering the humanized antibodies of the invention. The invention encompasses methods for treating an autoimmune disease and methods for elimination of cancer cells that express FcγRIIB.
摘要翻译：本发明涉及以与抗体结合FcγRIIA更大的亲和力结合人FcγRIIB的人源化FcγRIIB抗体，其片段和变体。本发明包括本发明的人源化抗体用于治疗与Fc受体介导的信号传导的平衡丧失有关的任何疾病（例如癌症，自身免疫性和炎症性疾病）的用途。本发明提供了通过施用本发明的人源化抗体来增强治疗性抗体的治疗效果以增强治疗性抗体的效应子功能的方法。本发明还提供了通过施用本发明的人源化抗体来增强疫苗组合物的功效的方法。本发明包括治疗自身免疫疾病的方法和用于消除表达FcγRIIB的癌细胞的方法。

83. 发明申请

US20070037216A1 Dual expression vector system for antibody expression in bacterial and mammalian cells 有权
标题翻译：用于细菌和哺乳动物细胞中抗体表达的双重表达载体系统
公开(公告)号：US20070037216A1
公开(公告)日：2007-02-15
申请号：US11503399
申请日：2006-08-10
申请人： Leslie Johnson , Ling Huang
发明人： Leslie Johnson , Ling Huang
IPC分类号： C40B30/06 , C40B50/06 , C12P21/06 , C07H21/04 , C12N1/21 , C12N5/08 , C12N5/06 , C12N15/74 , C07K16/18
CPC分类号： C07K16/00 , A01K2267/01 , C07K16/283 , C07K2317/10 , C07K2317/24 , C07K2317/51 , C07K2317/52 , C07K2317/55 , C07K2317/622 , C07K2319/30 , C12N7/00 , C12N15/8509 , C12N2795/14043
摘要： The present invention provides a dual expression vector, and methods for its use, for the expression and secretion of a full-length polypeptide of interest in eukaryotic cells, and a soluble domain or fragment of the polypeptide in bacteria. When expressed in bacteria, transcription from a bacterial promoter within a first intron and termination at the stop codon in a second intron results in expression of a fragment of the polypeptide, e.g., a Fab fragment, whereas in mammalian cells, splicing removes the bacterial regulatory sequences located in the two introns and generates the mammalian signal sequence, allowing expression of the full-length polypeptide, e.g., IgG heavy or light chain polypeptide. The dual expression vector system of the invention can be used to select and screen for new monoclonal antibodies, as well as to optimize monoclonal antibodies for binding to antigenic molecules of interest.
摘要翻译：本发明提供双重表达载体及其用于在真核细胞中表达和分泌全长多肽的方法，以及细菌中多肽的可溶性结构域或片段。当在细菌中表达时，来自第一内含子内的细菌启动子的转录和终止密码子在第二内含子中终止导致多肽片段的表达，例如Fab片段，而在哺乳动物细胞中，剪接除去细菌调节位于两个内含子中的序列并产生哺乳动物信号序列，允许表达全长多肽，例如IgG重链或轻链多肽。本发明的双表达载体系统可用于选择和筛选新的单克隆抗体，以及优化用于结合感兴趣的抗原分子的单克隆抗体。

84. 外观设计

USD961033S1 Inflatable toy 有权
公开(公告)号：USD961033S1
公开(公告)日：2022-08-16
申请号：US29794296
申请日：2021-06-11
申请人： Ling Huang
设计人： Ling Huang

85. 发明授权

US09494584B2 Signal enhancement system with multiple labeled-moieties 有权
标题翻译：具有多个标记部分的信号增强系统
公开(公告)号：US09494584B2
公开(公告)日：2016-11-15
申请号：US13165624
申请日：2011-06-21
申请人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： G01N33/558
CPC分类号： G01N33/558 , Y10S435/81 , Y10S436/807 , Y10S977/70 , Y10S977/773 , Y10S977/788 , Y10S977/918
摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
摘要翻译：描述了用于检测分析物的浸渍试验。在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。在捕获区存在胶体金标记表示测试溶液中存在分析物。使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化量油尺在一个单独的步骤。还描述了用于执行本发明的测试的套件。

86. 发明申请

US20120164396A1 MATRIX ASSISTED INK TRANSPORT 审中-公开
标题翻译： MATRIX辅助墨水运输
公开(公告)号：US20120164396A1
公开(公告)日：2012-06-28
申请号：US12140780
申请日：2008-06-17
申请人： Chad A. Mirkin , Ling Huang , Fengwei Huo , Sarah J. Hurst , Lidong Qin , Jae-Won Jang , Joseph J. Kakkassery
发明人： Chad A. Mirkin , Ling Huang , Fengwei Huo , Sarah J. Hurst , Lidong Qin , Jae-Won Jang , Joseph J. Kakkassery
IPC分类号： B05D5/00 , B32B3/10 , B82Y40/00
CPC分类号： C09D11/03 , B01J19/0046 , B01J2219/00382 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00648 , B01J2219/00653 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01J2219/00731 , B01J2219/00734 , B82Y30/00 , C09D11/30 , C40B50/14 , Y10T428/24802 , Y10T436/143333 , Y10T436/25 , Y10T436/2575
摘要： Provided is a direct write patterning method utilizing a mixture comprising an ink of choice and an ink carrier matrix. The method involves disposing the mixture on a tip or stamp and transporting the mixture from the tip or stamp on a surface to form a pattern that contains the ink. The method does not require chemical or physical modification of either the tip or stamp or the surface prior to transporting the mixture to the surface. The method can be applied for patterning hard inks such as nanomaterials and crystallized polymers and soft inks such as biomaterials including peptides and proteins. Also provided are related biomaterial and hard ink arrays.
摘要翻译：提供了一种使用包括选择的墨水和墨水载体基质的混合物的直接写入图案化方法。该方法包括将混合物设置在尖端或印模上，并将混合物从尖端或印模输送到表面上以形成包含油墨的图案。在将混合物输送到表面之前，该方法不需要对尖端或印模或表面进行化学或物理改性。该方法可以应用于诸如纳米材料和结晶聚合物的硬油墨和软油墨如包括肽和蛋白质的生物材料的图形化。还提供了相关的生物材料和硬油墨阵列。

87. 发明授权

US07972837B2 Signal enhancement system with multiple labeled-moieties 有权
标题翻译：具有多个标记部分的信号增强系统
公开(公告)号：US07972837B2
公开(公告)日：2011-07-05
申请号：US12042762
申请日：2008-03-05
申请人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： G01N33/53
CPC分类号： G01N33/558 , Y10S435/81 , Y10S436/807 , Y10S977/70 , Y10S977/773 , Y10S977/788 , Y10S977/918
摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
摘要翻译：描述了用于检测分析物的浸渍试验。在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。在捕获区存在胶体金标记表示测试溶液中存在分析物。使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化量油尺在一个单独的步骤。还描述了用于执行本发明的测试的套件。

88. 发明授权

US07906327B2 Dual expression vector system for antibody expression in bacterial and mammalian cells 有权
标题翻译：用于细菌和哺乳动物细胞中抗体表达的双重表达载体系统
公开(公告)号：US07906327B2
公开(公告)日：2011-03-15
申请号：US11503399
申请日：2006-08-10
申请人： Leslie Sydnor Johnson , Ling Huang
发明人： Leslie Sydnor Johnson , Ling Huang
IPC分类号： C12N15/00 , C12N1/21 , C12N5/07
CPC分类号： C07K16/00 , A01K2267/01 , C07K16/283 , C07K2317/10 , C07K2317/24 , C07K2317/51 , C07K2317/52 , C07K2317/55 , C07K2317/622 , C07K2319/30 , C12N7/00 , C12N15/8509 , C12N2795/14043
摘要： The present invention provides a dual expression vector, and methods for its use, for the expression and secretion of a full-length polypeptide of interest in eukaryotic cells, and a soluble domain or fragment of the polypeptide in bacteria. When expressed in bacteria, transcription from a bacterial promoter within a first intron and termination at the stop codon in a second intron results in expression of a fragment of the polypeptide, e.g., a Fab fragment, whereas in mammalian cells, splicing removes the bacterial regulatory sequences located in the two introns and generates the mammalian signal sequence, allowing expression of the full-length polypeptide, e.g., IgG heavy or light chain polypeptide. The dual expression vector system of the invention can be used to select and screen for new monoclonal antibodies, as well as to optimize monoclonal antibodies for binding to antigenic molecules of interest.
摘要翻译：本发明提供双重表达载体及其用于在真核细胞中表达和分泌全长多肽的方法，以及细菌中多肽的可溶性结构域或片段。当在细菌中表达时，来自第一内含子内的细菌启动子的转录和终止密码子在第二内含子中终止导致多肽片段的表达，例如Fab片段，而在哺乳动物细胞中，剪接除去细菌调节位于两个内含子中的序列并产生哺乳动物信号序列，允许表达全长多肽，例如IgG重链或轻链多肽。本发明的双表达载体系统可用于选择和筛选新的单克隆抗体，以及优化用于结合感兴趣的抗原分子的单克隆抗体。

89. 发明授权

US07776617B2 Signal enhancement system with multiple labeled-moieties 有权
标题翻译：具有多个标记部分的信号增强系统
公开(公告)号：US07776617B2
公开(公告)日：2010-08-17
申请号：US10432036
申请日：2001-11-30
申请人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
发明人： Helen Lee , Ling Huang , Magda Anastassova Dineva , Hsiang Yun Hu
IPC分类号： C12Q1/00 , C12M1/34 , C12M3/00 , G01N33/53 , G01N21/00 , G01N21/75 , G01N21/76 , G01N21/77 , G01N33/566 , G01N33/543 , G01N33/551 , G01N33/544
CPC分类号： G01N33/558 , Y10S435/81 , Y10S436/807 , Y10S977/70 , Y10S977/773 , Y10S977/788 , Y10S977/918
摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.
摘要翻译：描述了用于检测分析物的浸渍试验。在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。在捕获区存在胶体金标记表示测试溶液中存在分析物。使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化量油尺在一个单独的步骤。还描述了用于执行本发明的测试的套件。

90. 发明申请

US20100129908A1 SPACED PROJECTION SUBSTRATES AND DEVICES FOR CELL CULTURE 审中-公开
标题翻译：间隔投影基质和细胞培养装置
公开(公告)号：US20100129908A1
公开(公告)日：2010-05-27
申请号：US12622079
申请日：2009-11-19
申请人： Ye Fang , Yi-Cheng Hsieh , Ling Huang , Ying Wei
发明人： Ye Fang , Yi-Cheng Hsieh , Ling Huang , Ying Wei
IPC分类号： C12N5/071
CPC分类号： C12N5/067 , C12M25/02 , C12N2502/13 , C12N2533/54
摘要： An article for culturing cells includes a substrate on which cells can be cultured. The substrate has a base surface. An array of projections extends from the base surface. The projections have a height of about 1 micrometer to about 100 micrometers, and have a gap distance along the major surface from center to center between neighboring projections of about 10 micrometers to 80 micrometers. A plurality of arrays of projections may extend from the surface with gaps in the base surface between the arrays. Hepatocytes cultures on such microprojection array substrates maintained in vivo like morphology and membrane polarity. Hepatocytes co-cultured with helper cells on such substrates tended to grow in the area of the arrays, while the helper cells tended to grow in the areas between the arrays.
摘要翻译：培养细胞的制品包括可以培养细胞的底物。基板具有基面。一组突起从基面延伸出来。突起具有约1微米至约100微米的高度，并且在约10微米至80微米的相邻突起之间具有从中心到中心的主表面的间隙距离。多个突起阵列可以从阵列之间的基部表面中的间隙从表面延伸。在这样的微喷射阵列基板上的肝细胞培养物保持体内，如形态和膜极性。与这种底物上的辅助细胞共培养的肝细胞趋向于在阵列区域中生长，而辅助细胞趋向于在阵列之间的区域中生长。

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