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1. 发明申请

US20130280731A1 RATIONAL ENZYME MINING 有权
标题翻译：合理的采食
公开(公告)号：US20130280731A1
公开(公告)日：2013-10-24
申请号：US13989513
申请日：2011-11-29
申请人： Martin Karlsson , Bengt-Harald Jonsson , Uno Carlsson
发明人： Martin Karlsson , Bengt-Harald Jonsson , Uno Carlsson
IPC分类号： C12Q1/25
CPC分类号： C12Q1/25 , C12N1/36 , C12Q1/02
摘要： A method for rational mining for induced enzymes in microbial communities is described. The method is characterized in that a community of microorganisms is provided and that the microbial populations of the community are cultivated in a container under conditions of choice, where the microorganisms are given a defined culturing medium, to eliminate matter deriving from the natural habitat and to allow the microbial community to reach a metabolic steady-state. The method is further characterized in that at least one fraction of microorganisms is taken from the container and transferred into at least two separate containers, where at least one of the fractions of microorganisms is provided with a defined medium which includes an inducing substance and/or a substance against which enzyme/enzymes is/are desired, to induce regulation of expression of the desired enzyme/enzymes, and at least one fraction is provided with a defined medium without said inducing substance and without said substance against which enzyme/enzymes is/are desired, for the purpose of comparison, and the fractions are maintained and/or cultivated. Samples of the two fractions are then withdrawn and analyzed for identification of the induced enzyme/enzymes.
摘要翻译：描述了微生物群落中诱导酶合理开采的方法。该方法的特征在于提供微生物群落，并且在选择的条件下将容器中的微生物种群培养在容器中，其中微生物被赋予限定的培养基，以消除源于自然栖息地的物质，并且使微生物群落达到代谢稳态。该方法的特征还在于至少一部分微生物从容器中取出并转移到至少两个分开的容器中，其中至少一个微生物部分具有限定的培养基，其包括诱导物质和/或提供酶/酶所需的物质以诱导所需酶/酶的表达的调节，并且至少一个级分具有没有所述诱导物质的限定的培养基，并且没有所述酶/酶是/ 为了比较的目的，并且维持和/或培养级分。然后取出两个级分的样品并分析以鉴定诱导的酶/酶。

2. 发明授权

US09365887B2 Rational enzyme mining 有权
标题翻译：合理的酶开采
公开(公告)号：US09365887B2
公开(公告)日：2016-06-14
申请号：US13989513
申请日：2011-11-29
申请人： Martin Karlsson , Bengt-Harald Jonsson , Uno Carlsson
发明人： Martin Karlsson , Bengt-Harald Jonsson , Uno Carlsson
IPC分类号： C12Q1/25 , C12N1/36 , C12Q1/02
CPC分类号： C12Q1/25 , C12N1/36 , C12Q1/02
摘要： A method for rational mining for induced enzymes in microbial communities is described. The method is characterized in that a community of microorganisms is provided and that the microbial populations of the community are cultivated in a container under conditions of choice, where the microorganisms are given a defined culturing medium, to eliminate matter deriving from the natural habitat and to allow the microbial community to reach a metabolic steady-state. The method is further characterized in that at least one fraction of microorganisms is taken from the container and transferred into at least two separate containers, where at least one of the fractions of microorganisms is provided with a defined medium which includes an inducing substance and/or a substance against which enzyme/enzymes is/are desired, to induce regulation of expression of the desired enzyme/enzymes, and at least one fraction is provided with a defined medium without said inducing substance and without said substance against which enzyme/enzymes is/are desired, for the purpose of comparison, and the fractions are maintained and/or cultivated. Samples of the two fractions are then withdrawn and analyzed for identification of the induced enzyme/enzymes.
摘要翻译：描述了微生物群落中诱导酶合理开采的方法。该方法的特征在于提供微生物群落，并且在选择的条件下将容器中的微生物种群培养在容器中，其中微生物被赋予限定的培养基，以消除源于自然栖息地的物质，并且使微生物群落达到代谢稳态。该方法的特征还在于至少一部分微生物从容器中取出并转移到至少两个分开的容器中，其中至少一个微生物部分具有限定的培养基，其包括诱导物质和/或提供酶/酶所需的物质以诱导所需酶/酶的表达的调节，并且至少一个级分具有没有所述诱导物质的限定的培养基，并且没有所述酶/酶是/ 为了比较的目的，并且维持和/或培养级分。然后取出两个级分的样品并分析以鉴定诱导的酶/酶。

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