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    • 3. 发明授权
    • Method and computer program product for distinguishing and sorting seeds containing a genetic element of interest
    • 用于区分和分选含有遗传因素的种子的方法和计算机程序产品
    • US08452445B2
    • 2013-05-28
    • US12500847
    • 2009-07-10
    • Steven M. BeckerSteven J. CorakGary L. JaehnelHugh LuCatherine HunterCatherine Stevenson
    • Steven M. BeckerSteven J. CorakJames L. HunterGary L. JaehnelHugh LuJames W. Stevenson
    • C12Q1/68
    • B07C5/34B07C5/02B07C5/3425B07C5/3427G01N21/6428G01N21/85
    • A method for distinguishing and sorting seeds containing a genetic element of interest from a bulk sample. In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an photonic emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker. In some embodiments, the red fluorescent protein marker is discernable when excited by a certain energy and the evaluating step comprises exciting the seeds containing a genetic element of interest with the certain energy and detecting an emission resulting at least in part from the exciting step.
    • 用于从大量样品中区分并分选含有感兴趣遗传元件的种子的方法。 在各种实施方案中,本发明包括将标记物与含有本体样品的目的遗传元件的至少一些种子缔合,使用光子发射装置激发种子,评估批量样品的至少一些种子用于 标记的存在或不存在,以及基于标记的存在或不存在来分选含有感兴趣的遗传元件的种子。 在各种其它实施方案中,所述方法可以包括将红色荧光蛋白标记与至少一些含有本体样品的目的遗传元件的种子相关联,评估本体样品的至少一些种子以存在红色 使用评估装置的荧光蛋白标记物,并且基于红色荧光蛋白标记物的存在来分选含有遗传元件的种子。 在一些实施方案中,当被某些能量激发时,红色荧光蛋白标记物是可辨别的,并且评价步骤包括用特定能量激发含有感兴趣的遗传元件的种子,并且检测至少部分地从激发步骤得到的发射。