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    • 1. 发明授权
    • Method and computer program product for distinguishing and sorting seeds containing a genetic element of interest
    • 用于区分和分选含有遗传因素的种子的方法和计算机程序产品
    • US08452445B2
    • 2013-05-28
    • US12500847
    • 2009-07-10
    • Steven M. BeckerSteven J. CorakGary L. JaehnelHugh LuCatherine HunterCatherine Stevenson
    • Steven M. BeckerSteven J. CorakJames L. HunterGary L. JaehnelHugh LuJames W. Stevenson
    • C12Q1/68
    • B07C5/34B07C5/02B07C5/3425B07C5/3427G01N21/6428G01N21/85
    • A method for distinguishing and sorting seeds containing a genetic element of interest from a bulk sample. In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an photonic emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker. In some embodiments, the red fluorescent protein marker is discernable when excited by a certain energy and the evaluating step comprises exciting the seeds containing a genetic element of interest with the certain energy and detecting an emission resulting at least in part from the exciting step.
    • 用于从大量样品中区分并分选含有感兴趣遗传元件的种子的方法。 在各种实施方案中,本发明包括将标记物与含有本体样品的目的遗传元件的至少一些种子缔合,使用光子发射装置激发种子,评估批量样品的至少一些种子用于 标记的存在或不存在,以及基于标记的存在或不存在来分选含有感兴趣的遗传元件的种子。 在各种其它实施方案中,所述方法可以包括将红色荧光蛋白标记与至少一些含有本体样品的目的遗传元件的种子相关联,评估本体样品的至少一些种子以存在红色 使用评估装置的荧光蛋白标记物,并且基于红色荧光蛋白标记物的存在来分选含有遗传元件的种子。 在一些实施方案中,当被某些能量激发时,红色荧光蛋白标记物是可辨别的,并且评价步骤包括用特定能量激发含有感兴趣的遗传元件的种子,并且检测至少部分地从激发步骤得到的发射。
    • 2. 发明申请
    • METHOD AND COMPUTER PROGRAM PRODUCT FOR DISTINGUISHING AND SORTING SEEDS CONTAINING A GENETIC ELEMENT OF INTEREST
    • 用于分类和分类含有遗传基因元素的种子的方法和计算机程序产品
    • US20100143906A1
    • 2010-06-10
    • US12500847
    • 2009-07-10
    • Steven M. BeckerSteven J. CorakJames L. HunterGary L. JaehnelHugh LuJames W. Stevenson
    • Steven M. BeckerSteven J. CorakJames L. HunterGary L. JaehnelHugh LuJames W. Stevenson
    • C12Q1/68B07C5/02
    • B07C5/34B07C5/02B07C5/3425B07C5/3427G01N21/6428G01N21/85
    • A method for distinguishing and sorting seeds containing a genetic element of interest from a bulk sample. In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an photonic emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker. In some embodiments, the red fluorescent protein marker is discernable when excited by a certain energy and the evaluating step comprises exciting the seeds containing a genetic element of interest with the certain energy and detecting an emission resulting at least in part from the exciting step.
    • 用于从大量样品中区分并分选含有感兴趣遗传元件的种子的方法。 在各种实施方案中,本发明包括将标记物与含有本体样品的目的遗传元件的至少一些种子缔合,使用光子发射装置激发种子,评估批量样品的至少一些种子 标记的存在或不存在,以及基于标记的存在或不存在来分选含有感兴趣的遗传元件的种子。 在各种其它实施方案中,所述方法可以包括将红色荧光蛋白标记与至少一些含有本体样品的目的遗传元件的种子相关联,评估本体样品的至少一些种子以存在红色 使用评估装置的荧光蛋白标记物,并且基于红色荧光蛋白标记物的存在来分选含有遗传元件的种子。 在一些实施方案中,当被某些能量激发时,红色荧光蛋白标记物是可辨别的,并且评价步骤包括用特定能量激发含有感兴趣的遗传元件的种子,并且检测至少部分地从激发步骤得到的发射。
    • 5. 发明授权
    • System and method for sensor-based feedback control of a seed conditioning and production process
    • 种子调理和生产过程的基于传感器的反馈控制系统和方法
    • US09278377B2
    • 2016-03-08
    • US13289846
    • 2011-11-04
    • Thomas B. BrumbackSteven J. CorakJames L. HunterYong G. Lee
    • Thomas B. BrumbackSteven J. CorakJames L. HunterYong G. Lee
    • B65B1/04B65B3/04B65B57/00B65B1/32B07C5/00B07C5/16
    • B07C5/00
    • A system and method are provided for optimizing the flow of seed along a seed handling path. The system and method include multiple stages at which seeds undergo different processes to prepare the seeds for sale or further handling. The stages may include one or more of Receiving, Husking, Sorting, Drying, Shelling, Bulk Storage, Sizing, Conditioning, Treating, and Packaging. Sensors may be located along the seed handling path for monitoring the seed handling path and the operations and conveyances occurring along the seed handling path at and between different stages. The sensors may provide feedback signals to a controller, which may in turn adjust the operating parameters of various processing devices associated with one or more of the stages in a real-time scenario to optimize the operations and conveyances occurring along the seed handling path.
    • 提供了一种用于优化沿着种子处理路径的种子流动的系统和方法。 该系统和方法包括多个阶段,在此阶段,种子经历不同的处理以准备待种子或进一步处理的种子。 阶段可以包括接收,呼入,排序,干燥,剥壳,大容量存储,尺寸调节,处理和包装中的一个或多个。 传感器可以沿着种子处理路径定位,用于监测种子处理路径以及在不同阶段之间和之间沿着种子处理路径发生的操作和运输。 传感器可以向控制器提供反馈信号,控制器可以反过来在实时场景中调整与一个或多个阶段相关联的各种处理设备的操作参数,以优化沿种子处理路径发生的操作和运输。
    • 9. 发明申请
    • METHOD AND COMPUTER PROGRAM PRODUCT FOR DISTINGUISHING AND SORTING SEEDS CONTAINING A GENETIC ELEMENT OF INTEREST
    • 用于分类和分类含有遗传基因元素的种子的方法和计算机程序产品
    • US20090032441A1
    • 2009-02-05
    • US12108198
    • 2008-04-23
    • Steven J. CorakJames L. Hunter
    • Steven J. CorakJames L. Hunter
    • B07C5/34C12Q1/68G06F17/00
    • B07C5/3427B07C5/3425G01N21/6428G01N21/85
    • A method and computer program product for distinguishing and sort seeds containing a genetic element of interest from a bulk sample. In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an electromagnetic energy emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker using an evaluating device configured to excite a majority of the surface area of the seeds, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method and computer program product may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker. In some embodiments, the red fluorescent protein marker is discernable when excited by a certain energy and the evaluating step comprises exciting the seeds containing a genetic element of interest with the certain energy detecting an emission resulting at least in part from the exciting step.
    • 一种方法和计算机程序产品,用于从大量样品中区分并分类含有感兴趣的遗传元件的种子。 在各种实施方案中,本发明包括将标记物与含有本体样品的目的遗传元件的至少一些种子缔合,使用电磁能发射装置激发种子,评估大量样品的至少一些种子 用于使用被配置为激发种子的大部分表面积的评估装置的标记的存在或不存在,以及基于标记的存在或不存在来分选含有感兴趣的遗传元件的种子。 在各种其它实施方案中,所述方法和计算机程序产品可以包括将红色荧光蛋白标记与至少一些含有本体样品的目的遗传元件的种子相关联,评估批量样品的至少一些种子用于 使用评估装置存在红色荧光蛋白标记物,并根据红色荧光蛋白标记物的存在对含有遗传元件的种子进行分选。 在一些实施方案中,当被某些能量激发时,红色荧光蛋白标记物是可辨别的,并且评估步骤包括以特定能量激发含有感兴趣的遗传元件的种子,所述能量至少部分地从激发步骤检测出发射。