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    • 9. 发明申请
    • CANINE CD20 GENE
    • CANINE CD20基因
    • US20100136558A1
    • 2010-06-03
    • US12617571
    • 2009-11-12
    • Rui KANOAtsuhiko HASEGAWAChika INOUE
    • Rui KANOAtsuhiko HASEGAWAChika INOUE
    • C12Q1/68C07H21/02C12N15/74C12N1/21C07H21/04
    • C07K14/70596
    • It is intended to clarify the CD20 amino acid sequence and its gene sequence which are essentially required in constructing an anti-CD20 antibody useful in treating animal malignant lymphoma. It is also intended to provide a method of diagnosing canine malignant lymphoma by using the CD20 gene sequence. Using monocytes in canine blood as a sample, mRNA is obtained and the full base sequence of canine CD20 gene (SEQ ID NO:2) is determined. Based on this sequence, its amino acid sequence (SEQ ID NO:1) is determined. Comparing the homologies with human and mouse CD20 genes and amino acid sequences, it is identified as canine CD20 gene. Moreover, a primer specific to the canine CD20 gene is constructed and the expression of the CD20 gene in a sample is examined, thereby giving a method of diagnosing canine B lymphocyte-origin malignant lymphoma.
    • 旨在阐明构建可用于治疗动物恶性淋巴瘤的抗CD20抗体基本上需要的CD20氨基酸序列及其基因序列。 还旨在提供通过使用CD20基因序列诊断犬恶性淋巴瘤的方法。 以犬血液中的单核细胞作为样品,获得mRNA,并测定犬CD20基因(SEQ ID NO:2)的全碱基序列。 基于该序列,确定其氨基酸序列(SEQ ID NO:1)。 将同源性与人和小鼠CD20基因和氨基酸序列进行比较,鉴定为犬CD20基因。 此外,构建了特异于犬CD20基因的引物,并检查了样品中CD20基因的表达,从而给出了诊断犬B淋巴细胞起始恶性淋巴瘤的方法。