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1. 发明授权

US07592504B2 Methods for determining specificity of RNA silencing and for genetic analysis of the silenced gene or protein 有权
标题翻译：确定RNA沉默特异性和沉默基因或蛋白质遗传分析的方法
公开(公告)号：US07592504B2
公开(公告)日：2009-09-22
申请号：US11452821
申请日：2006-06-14
申请人： Daniel F. Klessig , Dhirendra Kumar
发明人： Daniel F. Klessig , Dhirendra Kumar
IPC分类号： C12N15/82 , C12N15/00
CPC分类号： C12N15/111 , C12N2310/14 , C12N2320/11
摘要： Methods and kits for determining the specificity of siRNAs for their targets are provided. Also provided is a method for performing genetic analysis of the target protein or gene using different versions of a synthetic gene to complement the phenotype induced by RNAi-mediated silencing of the target protein and/or gene of interest. Finally, a method for treating genetic disorders associated with production of mutated proteins is also disclosed.
摘要翻译：提供了确定siRNA靶向特异性的方法和试剂盒。还提供了使用不同版本的合成基因进行靶蛋白或基因的遗传分析的方法，以补充目标蛋白和/或感兴趣的基因的RNAi介导的沉默诱导的表型。最后，还公开了一种治疗与突变型蛋白质产生相关的遗传疾病的方法。

2. 发明授权

US5977442A Salicylic acid induced map kinase and its use for enhanced disease resistance in plants 失效
标题翻译：水杨酸诱导的地图激酶及其在植物中增强抗病性的用途
公开(公告)号：US5977442A
公开(公告)日：1999-11-02
申请号：US837593
申请日：1997-04-21
申请人： Daniel F. Klessig , Shugun Zhang
发明人： Daniel F. Klessig , Shugun Zhang
IPC分类号： C12N9/12 , C12N15/54 , C12N15/56 , C12N15/82 , C12N5/10 , C12N9/42
CPC分类号： C12N9/1205 , C12N15/8283
摘要： A salicylic acid-induced protein (SIP) kinase is disclosed. The kinase has a molecular weight of about 48 kDa and is activated in response to salicylic acid, H.sub.2 O.sub.2 and infection with tobacco mosaic virus. The activation and enzymatic properties of the purified protein have been characterized. The partial amino acid sequence and complete nucleotide sequence of a cDNA encoding the SIP kinase demonstrate that it is a unique member of the mitogen-activated protein (MAP) kinase family. The novel SIP kinase may play a critical role in signal transduction for activation of plant defenses against microbial pathogens.
摘要翻译：公开了A + E，uns s + EE脂酰酸+ E，uns i + EE诱导的+ E，uns p + EE蛋白（SIP）激酶。该激酶具有约48kDa的分子量，并响应于水杨酸，H 2 O 2和感染烟草花叶病毒而被激活。已经表征了纯化的蛋白质的活化和酶学性质。编码SIP激酶的cDNA的部分氨基酸序列和完整的核苷酸序列表明，它是+ E，uns m + EE itogen-+ E，uns a + EE激活+ E，uns p + EE蛋白的独特成员（MAP）激酶家族。新型SIP激酶可能在信号转导中起关键作用，用于激活植物防御微生物病原体。

3. 发明授权

US07169966B2 Salicylic acid-binding protein encoding nucleic acid, SABP2, and methods of use thereof 有权
标题翻译：水杨酸结合蛋白编码核酸，SABP2及其使用方法
公开(公告)号：US07169966B2
公开(公告)日：2007-01-30
申请号：US10780002
申请日：2004-02-17
申请人： Daniel F. Klessig , Dhirendra Kumar
发明人： Daniel F. Klessig , Dhirendra Kumar
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00
CPC分类号： C12N9/0065
摘要： Novel nucleic acid molecules encoding SA-binding proteins involved in SA-mediated disease resistance responses are disclosed. Methods of use of the nucleic acid molecules and proteins of the invention are also provided.
摘要翻译：公开了编码参与SA介导的疾病抗性反应的SA结合蛋白的新型核酸分子。还提供了本发明的核酸分子和蛋白质的使用方法。

4. 发明授权

US6136552A High-affinity salicylic acid-binding protein and methods of use 失效
标题翻译：高亲和力水杨酸结合蛋白及其使用方法
公开(公告)号：US6136552A
公开(公告)日：2000-10-24
申请号：US956507
申请日：1997-10-23
申请人： Daniel F. Klessig , He Du
发明人： Daniel F. Klessig , He Du
IPC分类号： C07K14/415 , G01N33/53
CPC分类号： C07K14/415
摘要： A high-affinity salicylic acid-binding protein (SABP2) derivable from tobacco and Arabidopsis is disclosed. The tobacco protein has a molecular weight of approximately 25 kDa and reversibly binds SA with an apparent K.sub.d of approximately 90 nM and a B.sub.max of 10 fmol/mg protein. The SABP2 of the invention may be used to identify analogues of SA. Analogues so identified may be used in plants to augment disease-resistance response pathways or other SA-sensitive processes in which SA plays a role. Possible examples include flowering and alternative respiration. The SABP2 of the invention may also be used to identify and clone a gene or cDNA that encodes it, which then may be used to generate transgenic plants having altered SABP2 levels.
摘要翻译：公开了一种可从烟草和拟南芥衍生的高亲和力水杨酸结合蛋白（SABP2）。烟草蛋白的分子量约为25kDa，可逆地结合SA，表观Kd为约90nM，Bmax为10fmol / mg蛋白。本发明的SABP2可以用于鉴定SA的类似物。如此鉴定的类似物可用于植物中以增强抗病反应途径或其中SA起作用的其他SA敏感过程。可能的例子包括开花和替代呼吸。本发明的SABP2还可以用于鉴定和克隆编码它的基因或cDNA，然后可以将其用于产生具有改变的SABP2水平的转基因植物。

5. 发明授权

US06495737B1 Methods and compositions for improving salicylic acid-independent systemic acquired disease resistance in plants 失效
标题翻译：用于改善植物中水杨酸独立系统获得性疾病抗性的方法和组合物
公开(公告)号：US06495737B1
公开(公告)日：2002-12-17
申请号：US08909125
申请日：1997-08-11
申请人： Daniel F. Klessig , Ailan Guo
发明人： Daniel F. Klessig , Ailan Guo
IPC分类号： C12N1582
CPC分类号： C12N15/8279 , C12N9/14
摘要： The present invention provides methods and materials that enhance a plant's resistance to certain pathogens. A novel pathway is described and has been designated with the acronym, SI-SAR pathway, for salicylic acid-independent systemic acquired resistance. DNA constructs and methodologies are provided that facilitate the identification of compounds that activate this pathway. Methods are provided to enable the identification of novel genes and signaling components that are expressed when the SI-SAR pathway is activated. Transgenic plants with altered expression of these novel genes or signaling components of the pathway are expected to have enhanced resistance to plant pathogens. Also provided is a novel, pathogen-induced epoxide hydrolase that is inducible in the absence of SA.
摘要翻译：本发明提供增强植物对某些病原体的抗性的方法和材料。描述了一条新途径，并且已经用缩写SI-SAR途径指定了水杨酸独立系统获得性抗性。提供DNA构建体和方法，其有助于鉴定激活该途径的化合物。提供了方法来确定当SI-SAR途径被激活时表达的新基因和信号传导成分。预期这些新基因或该途径的信号成分表达改变的转基因植物对植物病原体具有增强的抗性。还提供了一种新型的病原体诱导的环氧化物水解酶，其在不存在SA时可诱导。

6. 发明授权

US06765128B1 Method of using a pathogen-activatable map kinase to enhance disease resistance in plants 失效
标题翻译：使用病原体激活图谱激酶增强植物抗病性的方法
公开(公告)号：US06765128B1
公开(公告)日：2004-07-20
申请号：US09623034
申请日：2000-08-24
申请人： Daniel F. Klessig , Shugun Zhang
发明人： Daniel F. Klessig , Shugun Zhang
IPC分类号： C12N1582
CPC分类号： C12N9/1205
摘要： Novel used for WIPK, a member of the mitogen-activated protein (MAP) kinase family, are provided, based on the discovery that the WIPK protein is activatable in association with development or enhancement of resistance to microbial pathogens. Thus, WIPK may play a critical role in signal transduction for activation of plant defenses against certain microbial pathogens. Methods are disclosed for making WIPK transgenic plants with enchanced resistance to disease causing agents. In addition, transgenic plants transformed with WIPK and having enhanced disease resistance are disclosed.
摘要翻译：基于发现WIPK蛋白与发展或增强对微生物病原体的抗性相关联的发现，提供了用于WIPK的丝裂原活化蛋白（MAP）激酶家族成员的新颖性。因此，WIPK可能在用于激活针对某些微生物病原体的植物防御的信号转导中起关键作用。公开了制备具有对疾病引发剂具有增强抗性的WIPK转基因植物的方法。此外，公开了用WIPK转化并具有增强的抗病性的转基因植物。

7. 发明授权

US5939601A Genes associates with enhanced disease resistance in plants 失效
标题翻译：基因与植物抗病性增强有关
公开(公告)号：US5939601A
公开(公告)日：1999-08-17
申请号：US722626
申请日：1996-09-27
申请人： Daniel F. Klessig , Yinong Yang
发明人： Daniel F. Klessig , Yinong Yang
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , A01H4/00 , A01H5/10
CPC分类号： C12N15/8283 , C07K14/415 , C12N15/8282
摘要： An isolated nucleic acid molecule is provided which encodes a tobacco myb homologue involved in the regulation of disease resistance in plants. The encoded protein comprises a basic N-terminal region with two imperfect tryptophan repeats of 53 and 51 amino acids, a potential ATP/GTP binding site or P-loop, a redox sensitive cysteine and a nuclear localization sequence. The acidic C terminus of Myb1 forms amphipathic .alpha. helices which are characteristic of transcriptional activation domains. The invention also provides novel Myb1 protein and antibodies thereto. Additionally, the invention provides novel transgenic plants with enhanced disease resistance to certain pathogens.
摘要翻译：提供分离的核酸分子，其编码参与植物中抗病性调节的烟草myb同源物。编码的蛋白质包含具有53和51个氨基酸的两个不完全色氨酸重复的碱性N末端区域，潜在的ATP / GTP结合位点或P环，氧化还原敏感半胱氨酸和核定位序列。 Myb1的酸性C末端形成转录激活结构域特征的两亲性α螺旋。本发明还提供了新颖的Myb1蛋白及其抗体。另外，本发明提供对某些病原体具有增强的抗病性的新型转基因植物。

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