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1. 发明授权

US08830563B2 Laser scanning microscope having a laser diode comprising a light modulation device 有权
标题翻译：激光扫描显微镜，其具有包括光调制装置的激光二极管
公开(公告)号：US08830563B2
公开(公告)日：2014-09-09
申请号：US12999194
申请日：2009-06-12
申请人： Dieter Huhse , Stefan Wilhelm
发明人： Dieter Huhse , Stefan Wilhelm
IPC分类号： G02B21/00
CPC分类号： G02B21/0032 , G02B21/0076
摘要： The invention makes it possible to adjust the light intensity of a laser scanning microscope laser beam in an economical manner and with high accuracy. A separate acousto-optic component can be omitted in that a light modulation section such as an electroabsorption modulator (EAM) or a semiconductor amplifier (SOA) is arranged directly at the laser diode, advisably at one of its front sides. It is nevertheless possible to control the light intensity economically and with high accuracy because the important parameters of the laser beam remain unchanged when the optical output power is changed by the light modulation section. The light modulation section is preferably formed integral with the laser diode in at least one material layer.
摘要翻译：本发明能够以经济的方式和高精度地调节激光扫描显微镜激光束的光强度。可以省略单独的声光分量，因为诸如电吸收调制器（EAM）或半导体放大器（SOA）的光调制部分直接布置在激光二极管处，优选地在其前侧之一处。然而，当光输出功率被光调制部变化时，由于激光束的重要参数保持不变，所以可以经济地和高精度地控制光强度。光调制部分优选地在至少一个材料层中与激光二极管一体形成。

2. 发明申请

US20100027108A1 CONFOCAL LASER MICROSCOPE 有权
标题翻译：共焦激光显微镜
公开(公告)号：US20100027108A1
公开(公告)日：2010-02-04
申请号：US12522288
申请日：2008-02-09
申请人： Stefan Wilhelm , Dieter Huhse
发明人： Stefan Wilhelm , Dieter Huhse
IPC分类号： G02B21/06
CPC分类号： G02B21/0032 , G02B6/262
摘要： A confocal laser microscope has at least one laser whose illumination light is transmitted in direction of the microscope objective by at least one light-conducting fiber. The light-conducting fiber can be plugged in at a housing which preferably comprises the scanning head of the microscope, and a holder is provided which can be plugged into the housing and into which the light-conducting fiber projects and which is provided at its end remote of the fiber with first optics for transmitting the laser light exiting divergently from the fiber in direction of at least partially displaceable collimating optics in the housing. At least second optics are advantageously arranged between the first optics and the collimating optics.
摘要翻译：共焦激光显微镜具有至少一个激光器，其照明光通过至少一个光导纤维在显微镜物镜的方向上传播。导光纤维可以插入到优选包括显微镜的扫描头的壳体上，并且提供一个保持器，该保持器可以插入壳体中，并且光导纤维在其端部设置光纤的远端具有第一光学器件，用于传输沿着至少部分可位移的准直光学器件的方向从光纤发出的激光。至少第二光学器件有利地布置在第一光学器件和准直光学器件之间。

3. 发明申请

US20080043786A1 Tunable light source for use in microscopy 审中-公开
标题翻译：可调光源用于显微镜
公开(公告)号：US20080043786A1
公开(公告)日：2008-02-21
申请号：US11889906
申请日：2007-08-17
申请人： Stefan Wilhelm , Volker Gerstner , Peter Westphal , Dieter Huhse
发明人： Stefan Wilhelm , Volker Gerstner , Peter Westphal , Dieter Huhse
IPC分类号： H01S3/10
CPC分类号： G02B21/0032 , H01S5/06258 , H01S5/1215 , H01S5/36
摘要： A tunable lighting source, especially for a microscope, which contains a laser, in which the lighting source delivers spectrally variable and spatially coherent radiation. The tunable lighting source is based on a structured substrate coated with a laser medium, the structured substrate provided with the laser medium having a geometrically variable structure and delivering spatially coherent radiation by energy excitation.
摘要翻译：可调光源，特别是对于包含激光器的显微镜，其中照明源提供光谱可变和空间相干辐射。可调谐照明源基于涂覆有激光介质的结构化衬底，该结构化衬底设置有具有几何可变结构的激光介质，并且通过能量激发传送空间相干辐射。

4. 发明授权

US08553324B2 Microscope having internal focusing 有权
标题翻译：显微镜具有内部对焦
公开(公告)号：US08553324B2
公开(公告)日：2013-10-08
申请号：US12673682
申请日：2008-08-06
申请人： Joerg Pacholik , Marco Hanft , Dieter Huhse
发明人： Joerg Pacholik , Marco Hanft , Dieter Huhse
IPC分类号： G02B21/00
CPC分类号： G02B21/006 , G02B21/0048 , G02B21/0072 , G02B21/241
摘要： The invention relates to a laser scanning microscope with a scanner and a microscope objective, and to a control method for such a microscope. In order to obtain sharp imaging of the sample in a laser scanning microscope, the distance between the microscope objective and the sample is usually varied for adjusting the focus position. However, relative movements between the objective and the sample can be problematic. In view of the costly special objective, internal focusing of the objective is a disadvantageous solution. An improved laser scanning microscope should make it possible to sharply image a sample with standard objectives without relative movement between the microscope objective and sample. According to the invention, a tube lens is provided which is displaceable along the optical axis, and the focus position is adjustable relative to a front optical element of the microscope objective by adjusting the tube lens.
摘要翻译：本发明涉及具有扫描仪和显微镜物镜的激光扫描显微镜以及这种显微镜的控制方法。为了在激光扫描显微镜中获得样品的清晰成像，显微镜物镜与样品之间的距离通常是变化的，用于调节聚焦位置。然而，客观和样本之间的相对运动可能是有问题的。鉴于昂贵的特殊目标，目标的内部重点是不利的解决方案。改进的激光扫描显微镜应能使标准物体的样品锐利地成像，而不会在显微镜物镜和样品之间相对移动。根据本发明，提供了一种可沿着光轴移动的管透镜，并且通过调节管透镜，聚焦位置可相对于显微镜物镜的前光学元件调节。

5. 发明申请

US20100214653A1 MICROSCOPE HAVING INTERNAL FOCUSING 有权
标题翻译：具有内部聚焦的显微镜
公开(公告)号：US20100214653A1
公开(公告)日：2010-08-26
申请号：US12673682
申请日：2008-08-06
申请人： Joerg Pacholik , Marco Hanft , Dieter Huhse
发明人： Joerg Pacholik , Marco Hanft , Dieter Huhse
IPC分类号： G02B21/02
CPC分类号： G02B21/006 , G02B21/0048 , G02B21/0072 , G02B21/241
摘要： The invention relates to a laser scanning microscope with a scanner and a microscope objective, and to a control method for such a microscope. In order to obtain sharp imaging of the sample in a laser scanning microscope, the distance between the microscope objective and the sample is usually varied for adjusting the focus position. However, relative movements between the objective and the sample can be problematic. In view of the costly special objective, internal focusing of the objective is a disadvantageous solution. An improved laser scanning microscope should make it possible to sharply image a sample with standard objectives without relative movement between the microscope objective and sample. According to the invention, a tube lens is provided which is displaceable along the optical axis, and the focus position is adjustable relative to a front optical element of the microscope objective by adjusting the tube lens.
摘要翻译：本发明涉及具有扫描仪和显微镜物镜的激光扫描显微镜以及这种显微镜的控制方法。为了在激光扫描显微镜中获得样品的清晰成像，显微镜物镜与样品之间的距离通常是变化的，用于调节聚焦位置。然而，客观和样本之间的相对运动可能是有问题的。鉴于昂贵的特殊目标，目标的内部重点是不利的解决方案。改进的激光扫描显微镜应能使标准物体的样品锐利地成像，而不会在显微镜物镜和样品之间相对移动。根据本发明，提供了一种可沿着光轴移动的管透镜，并且通过调节管透镜，聚焦位置可相对于显微镜物镜的前光学元件调节。

6. 发明申请

US20060279272A1 Method for determining the frequency response of an electrooptical component 有权
标题翻译：确定电光元件频率响应的方法
公开(公告)号：US20060279272A1
公开(公告)日：2006-12-14
申请号：US10573116
申请日：2004-09-23
申请人： Dieter Huhse , Olaf Reimann
发明人： Dieter Huhse , Olaf Reimann
IPC分类号： G01R31/00
CPC分类号： G01R23/16 , G01R31/2635
摘要： The aim of the invention is to provide a method for determining the frequency response of an electrooptical component, particularly, for example, of a light-generating or light-modulating component, which is easy to carry out. To this end, the invention provides a method during which optical pulses with a pulse frequency (fp) are generated. The electrooptical component (60) is controlled by an electrical measuring signal (Smess) with a measuring frequency (fmess) in such a manner that an optical output signal (Saus) is formed that is modulated with the measuring frequency (fmess). The measuring frequency (fmess) is equal to an integral multiple of the pulse frequency (fp) plus a predetermined frequency offset ($g(D)f). The pulses and the output signal (Saus) are mixed, and a mixed product (M) is detected whose modulation frequency corresponds to the predetermined frequency offset ($g(D)f). The mixed product indicates the frequency response of the electrooptical component (60) at the measuring frequency (fmess).
摘要翻译：本发明的目的是提供一种用于确定电光部件的频率响应的方法，特别是例如易于进行的发光或光调制部件的频率响应。为此，本发明提供了一种产生具有脉冲频率（fp）的光脉冲的方法。电光分量（60）由具有测量频率（fmess）的电测信号（Smess）控制，使得形成以测量频率（fmess）调制的光输出信号（Saus）。测量频率（fmess）等于脉冲频率（fp）加预定频率偏移（$ g（D）f）的整数倍。脉冲和输出信号（Saus）被混合，并且检测其调制频率对应于预定频率偏移（$ g（D）f）的混合乘积（M）。混合产品表示电光成分（60）在测量频率（fmess）下的频率响应。

7. 发明申请

US20120229815A1 LASER SCANNING MICROSCOPE AND METHOD FOR OPERATION THEREOF 有权
标题翻译：激光扫描显微镜及其操作方法
公开(公告)号：US20120229815A1
公开(公告)日：2012-09-13
申请号：US13413960
申请日：2012-03-07
申请人： NILS LANGHOLZ , Dieter Huhse
发明人： NILS LANGHOLZ , Dieter Huhse
IPC分类号： G01B11/24
CPC分类号： G02B21/008 , G01N21/6458 , G02B21/0076 , G06T5/50 , G06T2207/10061 , G06T2207/10144 , G06T2207/20208 , G06T2207/20216
摘要： Laser scanning microscope and method for the operation thereof having at least two detection channels which has at least one beamsplitter with a splitting of the sample light deviating from the 50:50 split and/or, with 50:50 split in the detection channels, has detectors with differently adjusted gain, or in at least one detection channel with equal light splitting has an additional light attenuator.
摘要翻译：激光扫描显微镜及其具有至少两个检测通道的检测通道的方法，该检测通道具有至少一个分光器，该分束器与50:50分裂偏离的样品光分离和/或在检测通道中以50:50的间隔分开具有不同调节增益的检测器，或者在具有相同光分裂的至少一个检测通道中具有附加的光衰减器。

8. 发明授权

US07522285B2 Method for determining the frequency response of an electrooptical component 有权
标题翻译：确定电光元件频率响应的方法
公开(公告)号：US07522285B2
公开(公告)日：2009-04-21
申请号：US10573116
申请日：2004-09-23
申请人： Dieter Huhse , Olaf Reimann
发明人： Dieter Huhse , Olaf Reimann
IPC分类号： G01B9/02
CPC分类号： G01R23/16 , G01R31/2635
摘要： The aim of the invention is to provide a method for determining the frequency response of an electrooptical component, particularly, for example, of a light-generating or light-modulating component, which is easy to carry out. To this end, the invention provides a method during which optical pulses with a pulse frequency (fp) are generated. The electrooptical component (60) is controlled by an electrical measuring signal (Smess) with a measuring frequency (fmess) in such a manner that an optical output signal (Saus) is formed that is modulated with the measuring frequency (fmess). The measuring frequency (fmess) is equal to an integral multiple of the pulse frequency (fp) plus a predetermined frequency offset ($g(D)f). The pulses and the output signal (Saus) are mixed, and a mixed product (M) is detected whose modulation frequency corresponds to the predetermined frequency offset ($g(D)f). The mixed product indicates the frequency response of the electrooptical component (60) at the measuring frequency (fmess).
摘要翻译：本发明的目的是提供一种用于确定电光部件的频率响应的方法，特别是例如易于进行的发光或光调制部件的频率响应。为此，本发明提供了一种产生具有脉冲频率（fp）的光脉冲的方法。电光分量（60）由具有测量频率（fmess）的电测信号（Smess）控制，使得形成以测量频率（fmess）调制的光输出信号（Saus）。测量频率（fmess）等于脉冲频率（fp）加预定频率偏移（$ g（D）f）的整数倍。脉冲和输出信号（Saus）被混合，并且检测其调制频率对应于预定频率偏移（$ g（D）f）的混合乘积（M）。混合产品表示电光成分（60）在测量频率（fmess）下的频率响应。

9. 发明授权

US08879072B2 Laser scanning microscope and method for operation thereof 有权
标题翻译：激光扫描显微镜及其操作方法
公开(公告)号：US08879072B2
公开(公告)日：2014-11-04
申请号：US13413960
申请日：2012-03-07
申请人： Nils Langholz , Dieter Huhse
发明人： Nils Langholz , Dieter Huhse
IPC分类号： G01B11/24 , G01N21/64 , G06T5/50 , G02B21/00
CPC分类号： G02B21/008 , G01N21/6458 , G02B21/0076 , G06T5/50 , G06T2207/10061 , G06T2207/10144 , G06T2207/20208 , G06T2207/20216
摘要： Laser scanning microscope and method for the operation thereof having at least two detection channels which has at least one beamsplitter with a splitting of the sample light deviating from the 50:50 split and/or, with 50:50 split in the detection channels, has detectors with differently adjusted gain, or in at least one detection channel with equal light splitting has an additional light attenuator.
摘要翻译：激光扫描显微镜及其具有至少两个检测通道的检测通道的方法，该检测通道具有至少一个分光器，该分束器与50:50分裂偏离的样品光分离和/或在检测通道中以50:50的间隔分开具有不同调节增益的检测器，或者在具有相同光分裂的至少一个检测通道中具有附加的光衰减器。

10. 发明授权

US08477418B2 Confocal laser microscope 有权
标题翻译：共焦激光显微镜
公开(公告)号：US08477418B2
公开(公告)日：2013-07-02
申请号：US12522288
申请日：2008-02-09
申请人： Stefan Wilhelm , Dieter Huhse
发明人： Stefan Wilhelm , Dieter Huhse
IPC分类号： G02B6/32 , G02B21/06
CPC分类号： G02B21/0032 , G02B6/262
摘要： A confocal laser microscope has at least one laser whose illumination light is transmitted in direction of the microscope objective by at least one light-conducting fiber. The light-conducting fiber can be plugged in at a housing which preferably comprises the scanning head of the microscope, and a holder is provided which can be plugged into the housing and into which the light-conducting fiber projects and which is provided at its end remote of the fiber with first optics for transmitting the laser light exiting divergently from the fiber in direction of at least partially displaceable collimating optics in the housing. At least second optics are advantageously arranged between the first optics and the collimating optics.
摘要翻译：共焦激光显微镜具有至少一个激光器，其照明光通过至少一个光导纤维在显微镜物镜的方向上传播。导光纤维可以插入到优选包括显微镜的扫描头的壳体上，并且提供一个保持器，该保持器可以插入壳体中，并且光导纤维在其端部设置光纤的远端具有第一光学器件，用于传输沿着至少部分可位移的准直光学器件的方向从光纤发出的激光。至少第二光学器件有利地布置在第一光学器件和准直光学器件之间。

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