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1. 发明申请

US20120302733A1 SELECTION AND CHARACTERIZATION OF NOVEL PLANT-DERIVED RECOMBINANT HUMAN INTERFERONS WITH BROAD SPECTRUM ACTIVITY 有权
标题翻译：新型植物衍生重组人类干扰素与广谱光谱活性的选择和表征
公开(公告)号：US20120302733A1
公开(公告)日：2012-11-29
申请号：US13478330
申请日：2012-05-23
申请人： Hal S. Padgett , Fakhrieh S. Vojdani , Andrew A. Vaewhongs
发明人： Hal S. Padgett , Fakhrieh S. Vojdani , Andrew A. Vaewhongs
IPC分类号： C07K14/555
CPC分类号： C07K14/555 , C07K14/56 , C07K2319/04 , C07K2319/21 , C12N15/8257
摘要： Methods to derive novel hybrid type 1 interferons that are broadly active against highly pathogenic viruses of biodefense significance are described. Libraries of hybrid interferon genes were produced using gene shuffling, the proteins were expressed, and screened for activity against viruses of interest. Sequences of several broadly active hybrid interferons are described.
摘要翻译：描述了衍生对具有生物防御重要性的高致病性病毒广泛有效的新型杂合1型干扰素的方法。使用基因改组产生杂交干扰素基因的文库，对蛋白进行表达，筛选出针对目的病毒的活性。描述了几种广泛活性的混合干扰素的序列。

2. 发明授权

US07217514B2 Method of increasing complementarity in a heteroduplex 有权
标题翻译：在异源双链中增加互补性的方法
公开(公告)号：US07217514B2
公开(公告)日：2007-05-15
申请号：US10206030
申请日：2002-07-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
摘要翻译：我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。该方法使用相反链的退火以形成具有错配的多核苷酸双链体。将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。任选地连接得到的多核苷酸。产生几种变异多核苷酸。在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。在退火异源链之前，母体多核苷酸不需要切割成片段。因此，不需要重新组装。

3. 发明申请

US20130137601A1 LAMINAR LIBRARY SCREEN 审中-公开
标题翻译： LAMINAR图书馆屏幕
公开(公告)号：US20130137601A1
公开(公告)日：2013-05-30
申请号：US13752381
申请日：2013-01-28
申请人： Hal S. Padgett
发明人： Hal S. Padgett
IPC分类号： C12N15/10
CPC分类号： C12N15/1086 , C12N15/1034 , C12Q1/025 , G01N33/5097 , G01N2500/10
摘要： Described is a method of screening libraries of variant proteins produced in plant leaves using a plant viral vector to identify a gene of interest comprising, inoculating leaves with a library of viruses expressing variant genes, allowing time for infected foci to form, harvesting a leaf, sticking one face of the leaf to a sticky support material to immobilize the leaf and leaving the opposing face of the leaf exposed, abrading the exposed face with granular material, placing the abraded face in contact with a blot membrane having a backing comprising blotting paper, placing the assembly into a vacuum seal bag; evacuating and sealing the bag; removing the assembly and separating the membrane, performing an assay on the membrane to identify an infected focus of interest; recovering virus corresponding to the infected focus; recovering nucleic acid from the virus, and identifying the gene of interest from the nucleic acid.
摘要翻译：描述了使用植物病毒载体筛选在植物叶中产生的变体蛋白的文库的方法，以鉴定感兴趣的基因，包括用表达变体基因的病毒文库接种叶，允许感染病灶形成时间，收获叶，将叶片的一个表面粘附到粘性支撑材料上以固定叶片并使叶子的相对面暴露，用颗粒材料研磨暴露的面，将磨损的面与带有背衬的印迹膜接触，包括吸印纸，将组件放入真空密封袋中; 撤离和密封袋子; 去除组件并分离膜，在膜上进行测定以鉴定感兴趣的焦点; 恢复对应感染重点的病毒; 从病毒中回收核酸，以及从核酸鉴定感兴趣的基因。

4. 发明授权

US07582423B2 Population of polynucleotide sequence variants 失效
标题翻译：多核苷酸序列变体种群
公开(公告)号：US07582423B2
公开(公告)日：2009-09-01
申请号：US10280913
申请日：2002-10-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.
摘要翻译：我们在这里描述了通过从两个不相同的多核苷酸制备异源双链多核苷酸来重新分配不相同的多核苷酸序列之间的序列变异的体外方法; 在碱基对失配位点处或附近在一条链中引入切口; 从错配发生的不匹配位置去除错配的碱基; 并使用相反的链作为模板以用第一链中的碱基补充的碱替换去除的碱基。通过这种方法，信息在不匹配的位置从一条链路转移到另一条链路。

5. 发明授权

US07078211B2 Nucleic acid molecules encoding endonucleases and methods of use thereof 失效
公开(公告)号：US07078211B2
公开(公告)日：2006-07-18
申请号：US10211079
申请日：2002-08-01
申请人： Hal S. Padgett , Andrew A. Vaewhongs
发明人： Hal S. Padgett , Andrew A. Vaewhongs
IPC分类号： C12N15/55 , C12N9/22
CPC分类号： C12N15/102 , C07K14/005 , C12N9/22 , C12N15/1027 , C12N15/8203 , C12N15/8257 , C12N2770/00022
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

6. 发明申请

US20130295131A1 GENERATION OF ANTIGENIC VIRUS-LIKE PARTICLES THROUGH PROTEIN-PROTEIN LINKAGES 审中-公开
标题翻译：通过蛋白质蛋白链接产生抗原性病毒颗粒
公开(公告)号：US20130295131A1
公开(公告)日：2013-11-07
申请号：US13883459
申请日：2010-11-05
申请人： Hal S. Padgett
发明人： Hal S. Padgett
IPC分类号： A61K39/21
CPC分类号： A61K39/21 , C07K14/005 , C07K14/43581 , C07K14/70596 , C07K2319/00 , C12N7/00 , C12N2770/00022 , C12N2770/00023
摘要： We have generated virus-like particles (VLPs) that can display other proteins through covalent protein-protein linkages mediated by the ‘Dock and Lock’ interaction between the Drosophila NorpA protein and the C-terminal pentapeptide tail of the InaD protein. This interaction may also be mediated by a portion of the SITAC protein and the Tetraspanin L6 Antigen protein. This system can be used to generate high-density scaffolded arrays of epitopes for immunization. This technology can streamline VLP vaccine candidate production, making it possible to rapidly evaluate panels of candidates in response to current vaccine needs and emerging pathogen threats.
摘要翻译：我们已经产生病毒样颗粒（VLPs），可以通过果蝇NorpA蛋白和InaD蛋白的C末端五肽尾部之间的“Dock和Lock”相互作用介导的共价蛋白质 - 蛋白质键显示其他蛋白质。这种相互作用也可能由SITAC蛋白和四肽蛋白L6抗原蛋白的一部分介导。该系统可用于产生用于免疫的表位的高密度支架阵列。该技术可以简化VLP疫苗候选生产，可以根据目前的疫苗需求和新出现的病原体威胁快速评估候选人小组。

7. 发明申请

US20110111413A1 METHOD OF OPTIMIZING CODON USAGE THROUGH DNA SHUFFLING 审中-公开
标题翻译：通过DNA切片优化CODON使用的方法
公开(公告)号：US20110111413A1
公开(公告)日：2011-05-12
申请号：US12952209
申请日：2010-11-23
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
IPC分类号： C12Q1/68
CPC分类号： C12N15/102 , C12N15/1027
摘要： The present invention relates to codon optimization utilizing DNA shuffling. A method of producing gene sequences optimized for a desired functional property is described involving synthesizing a library of parental codon variant genes encoding some or all codon choices at some or all amino acid positions of a gene, reassorting the variant codons among the parental codon variant genes using DNA shuffling thereby forming progeny codon variant genes, expressing the progeny codon variant genes in a host; and screening or selecting for progeny codon variant genes encoding a desired functional property.
摘要翻译：本发明涉及使用DNA改组的密码子优化。描述了产生针对所需功能性质优化的基因序列的方法，其涉及在基因的某些或所有氨基酸位置合成编码一些或所有密码子选择的亲代密码子变体基因的文库，重排亲代密码子变体基因中的变体密码子使用DNA改组从而形成子代密码子变体基因，在宿主中表达后代密码子变体基因; 以及筛选或选择编码期望功能特性的后代密码子变体基因。

8. 发明授权

US07888475B2 Interferon alpha and interferon kappa fusion protein 有权
标题翻译：干扰素α和干扰素κ融合蛋白
公开(公告)号：US07888475B2
公开(公告)日：2011-02-15
申请号：US12560391
申请日：2009-09-15
申请人： Hal S. Padgett , Fakhrieh S. Vojdani , Andrew A. Vaewhongs
发明人： Hal S. Padgett , Fakhrieh S. Vojdani , Andrew A. Vaewhongs
IPC分类号： C07K17/00 , C12N15/00 , C07H21/04
CPC分类号： C07K14/555 , C12N2710/24011
摘要： Herein is described a system to combat poxvirus infection wherein antagonists are developed that bind the soluble cytokine receptor but have no significant biological activity in the host, effectively blocking the virus-mediated suppressor of interferon function, thereby permitting the host's own cytokines to stimulate an antiviral response. Alternatively, interferon molecules can be developed that retain biological activity on their native receptors but fail to bind the viral cytokine binding protein, thereby circumventing this virus immune modulation mechanism.
摘要翻译：本文描述了一种防止痘病毒感染的系统，其中开发出拮抗剂结合可溶性细胞因子受体但在宿主中没有显着生物学活性的系统，有效阻断病毒介导的干扰素功能抑制因子，由此允许宿主自身的细胞因子刺激抗病毒响应。或者，可以开发干扰素分子，其保留对其天然受体的生物活性，但不能结合病毒细胞因子结合蛋白，从而规避该病毒免疫调节机制。

9. 发明授权

US07838219B2 Method of increasing complementarity in a heteroduplex 有权
标题翻译：在异源双链中增加互补性的方法
公开(公告)号：US07838219B2
公开(公告)日：2010-11-23
申请号：US10637758
申请日：2003-08-08
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/1027 , C12N15/102
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
摘要翻译：我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。该方法使用相反链的退火以形成具有错配的多核苷酸双链体。将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。任选地连接得到的多核苷酸。产生几种变异多核苷酸。在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。在退火异源链之前，母体多核苷酸不需要切割成片段。因此，不需要重新组装。

10. 发明授权

US07833759B2 Method of increasing complementarity in a heteroduplex 有权
公开(公告)号：US07833759B2
公开(公告)日：2010-11-16
申请号：US11768157
申请日：2007-06-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

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