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1. 发明授权

US07582423B2 Population of polynucleotide sequence variants 失效
标题翻译：多核苷酸序列变体种群
公开(公告)号：US07582423B2
公开(公告)日：2009-09-01
申请号：US10280913
申请日：2002-10-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.
摘要翻译：我们在这里描述了通过从两个不相同的多核苷酸制备异源双链多核苷酸来重新分配不相同的多核苷酸序列之间的序列变异的体外方法; 在碱基对失配位点处或附近在一条链中引入切口; 从错配发生的不匹配位置去除错配的碱基; 并使用相反的链作为模板以用第一链中的碱基补充的碱替换去除的碱基。通过这种方法，信息在不匹配的位置从一条链路转移到另一条链路。

2. 发明申请

US20110111413A1 METHOD OF OPTIMIZING CODON USAGE THROUGH DNA SHUFFLING 审中-公开
标题翻译：通过DNA切片优化CODON使用的方法
公开(公告)号：US20110111413A1
公开(公告)日：2011-05-12
申请号：US12952209
申请日：2010-11-23
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
IPC分类号： C12Q1/68
CPC分类号： C12N15/102 , C12N15/1027
摘要： The present invention relates to codon optimization utilizing DNA shuffling. A method of producing gene sequences optimized for a desired functional property is described involving synthesizing a library of parental codon variant genes encoding some or all codon choices at some or all amino acid positions of a gene, reassorting the variant codons among the parental codon variant genes using DNA shuffling thereby forming progeny codon variant genes, expressing the progeny codon variant genes in a host; and screening or selecting for progeny codon variant genes encoding a desired functional property.
摘要翻译：本发明涉及使用DNA改组的密码子优化。描述了产生针对所需功能性质优化的基因序列的方法，其涉及在基因的某些或所有氨基酸位置合成编码一些或所有密码子选择的亲代密码子变体基因的文库，重排亲代密码子变体基因中的变体密码子使用DNA改组从而形成子代密码子变体基因，在宿主中表达后代密码子变体基因; 以及筛选或选择编码期望功能特性的后代密码子变体基因。

3. 发明授权

US07838219B2 Method of increasing complementarity in a heteroduplex 有权
标题翻译：在异源双链中增加互补性的方法
公开(公告)号：US07838219B2
公开(公告)日：2010-11-23
申请号：US10637758
申请日：2003-08-08
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice , Andrew A. Vaewhongs
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/1027 , C12N15/102
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
摘要翻译：我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。该方法使用相反链的退火以形成具有错配的多核苷酸双链体。将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。任选地连接得到的多核苷酸。产生几种变异多核苷酸。在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。在退火异源链之前，母体多核苷酸不需要切割成片段。因此，不需要重新组装。

4. 发明授权

US07833759B2 Method of increasing complementarity in a heteroduplex 有权
公开(公告)号：US07833759B2
公开(公告)日：2010-11-16
申请号：US11768157
申请日：2007-06-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

5. 发明授权

US07235386B2 Method of increasing complementarity in a heteroduplex 有权
公开(公告)号：US07235386B2
公开(公告)日：2007-06-26
申请号：US10205772
申请日：2002-07-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.

6. 发明授权

US08936937B2 System for expression of genes in plants from a virus-based expression vector 有权
标题翻译：用于从基于病毒的表达载体表达植物中的基因的系统
公开(公告)号：US08936937B2
公开(公告)日：2015-01-20
申请号：US12524812
申请日：2008-01-28
申请人： John A. Lindbo
发明人： John A. Lindbo
IPC分类号： C12N15/40 , C12N15/82 , C12N5/10 , C12N15/64 , C12N15/67
CPC分类号： C12N15/8203 , C12N15/8205
摘要： Modified expression vectors, including Tobacco Mosaic Virus (TMV) expression vectors, methods for modifying such vectors, and uses of the same are disclosed.
摘要翻译：公开了包括烟草花叶病毒（TMV）表达载体，修饰这些载体的方法及其用途的修饰的表达载体。

7. 发明授权

US07413889B2 Production of a parvovirus vaccine in plants as viral coat protein fusions 失效
标题翻译：在病毒外壳蛋白融合物中生产细菌病毒疫苗
公开(公告)号：US07413889B2
公开(公告)日：2008-08-19
申请号：US10061216
申请日：2002-02-04
申请人： Gregory P. Pogue , John A. Lindbo , Michael J. McCulloch , Jonathan E. Lawrence , Cynthia S. Gross , Stephen J. Garger
发明人： Gregory P. Pogue , John A. Lindbo , Michael J. McCulloch , Jonathan E. Lawrence , Cynthia S. Gross , Stephen J. Garger
IPC分类号： C12N7/04
CPC分类号： C07K14/005 , A61K39/00 , C07K14/445 , C07K2319/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , C12N2770/00022
摘要： The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.
摘要翻译：本发明涉及与重组植物病毒结构蛋白融合的外来肽序列及其生产方法。融合蛋白在生物学含有的烟草花叶病毒的高水平植物中经济合成。本发明的融合蛋白可用作诱导产生具有所需结合特性的抗体的抗原，例如保护性抗体，或用作诱导针对细小病毒的保护性免疫的疫苗抗原。将猫细小病毒表位融合到TMV外壳蛋白的N末端，在烟草植物中表达，提取，纯化，表征并施用于动物，导致保护性免疫。

8. 发明授权

US07270825B2 Production of a parvovirus vaccine in plants as viral coat protein fusions 失效
标题翻译：在病毒外壳蛋白融合物中生产细菌病毒疫苗
公开(公告)号：US07270825B2
公开(公告)日：2007-09-18
申请号：US10193142
申请日：2002-07-12
申请人： Gregory P. Pogue , John A. Lindbo , Michael J. McCulloch , Jonathan E. Lawrence , Cynthia S. Gross , Stephen J. Garger
发明人： Gregory P. Pogue , John A. Lindbo , Michael J. McCulloch , Jonathan E. Lawrence , Cynthia S. Gross , Stephen J. Garger
IPC分类号： A61K39/23 , A61K39/12 , A61K38/00
CPC分类号： C07K14/005 , A61K39/00 , C07K14/445 , C07K2319/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , C12N2770/00022
摘要： The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.
摘要翻译：本发明涉及与重组植物病毒结构蛋白融合的外来肽序列及其生产方法。融合蛋白在生物学含有的烟草花叶病毒的高水平植物中经济合成。本发明的融合蛋白可用作诱导产生具有所需结合特性的抗体的抗原，例如保护性抗体，或用作诱导针对细小病毒的保护性免疫的疫苗抗原。将猫细小病毒表位融合到TMV外壳蛋白的N末端，在烟草植物中表达，提取，纯化，表征并施用于动物，导致保护性免疫。

9. 发明授权

US07217514B2 Method of increasing complementarity in a heteroduplex 有权
标题翻译：在异源双链中增加互补性的方法
公开(公告)号：US07217514B2
公开(公告)日：2007-05-15
申请号：US10206030
申请日：2002-07-25
申请人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12N15/102 , C12N15/1027
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
摘要翻译：我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。该方法使用相反链的退火以形成具有错配的多核苷酸双链体。将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。任选地连接得到的多核苷酸。产生几种变异多核苷酸。在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。在退火异源链之前，母体多核苷酸不需要切割成片段。因此，不需要重新组装。

10. 发明授权

US07939318B2 Flexible vaccine assembly and vaccine delivery platform 有权
标题翻译：灵活的疫苗组装和疫苗交付平台
公开(公告)号：US07939318B2
公开(公告)日：2011-05-10
申请号：US11410572
申请日：2006-04-24
申请人： Alison A. McCormick , Mark L. Smith , Kenneth E. Palmer , John A. Lindbo , Long V. Nguyen , Gregory P. Pogue
发明人： Alison A. McCormick , Mark L. Smith , Kenneth E. Palmer , John A. Lindbo , Long V. Nguyen , Gregory P. Pogue
IPC分类号： C12N15/63 , A61K35/00
CPC分类号： C07K14/005 , A61K39/0011 , A61K39/12 , A61K39/385 , A61K2039/5258 , A61K2039/55516 , A61K2039/6075 , A61K2039/627 , A61K2039/64 , C07K14/4748 , C07K2319/00 , C12N7/00 , C12N2710/20022 , C12N2710/20034 , C12N2760/16122 , C12N2760/18022 , C12N2770/00022 , C12N2770/00023
摘要： Herein-described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different encapsidation intermediate such that the reassembled VLP displays more than one peptide or nucleic acid. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.
摘要翻译：本文描述了制备由重组病毒颗粒（VLP）制成的疫苗的各种方法。首先，将VLP分解成壳化中间种群。每个壳化中间体经历例如独特的肽或核酸部分的化学共轭以形成分开的群体。此后，将来自不同群体的几种（一种或多种）不同的壳化中间体中的每一种的预定量混合并连接，形成围绕核酸核心的完整的VLP，其由不同的包封中间体组成，使得重新组装的VLP显示多于一种肽或核酸。核酸可以作为单独的支架起作用，或者可以被工程化以在真核细胞中表达免疫调节蛋白。

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