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1. 发明授权

US06982143B1 Normalizing and amplifying RNA 失效
标题翻译：归一化和扩增RNA
公开(公告)号：US06982143B1
公开(公告)日：2006-01-03
申请号：US09597608
申请日：2000-06-20
申请人： John Ngai , David Lin
发明人： John Ngai , David Lin
IPC分类号： C12Q1/68 , C12Q1/48 , C12P19/34 , C07H21/04
CPC分类号： C12N15/1096
摘要： The invention provides methods and compositions for normalizing and amplifying RNA populations. The methods generally comprise the steps of copying message RNA (mRNA) to form first single-stranded (ss) cDNA; converting the first ss-cDNA to first double-stranded (ds) cDNA; linearly amplifying the first ds-cDNA to form first amplified RNA (aRNA); tagging the 3′ end of the first aRNA with a known sequence to form 3′-tagged first aRNA; copying the 3′-tagged first aRNA to form second ss-cDNA; and normalizing the mRNA and/or the first aRNA.
摘要翻译：本发明提供了用于使RNA群体正常化和扩增的方法和组合物。所述方法通常包括复制消息RNA（mRNA）以形成第一单链（ss）cDNA的步骤; 将第一个ss-cDNA转化为第一个双链（ds）cDNA; 线性扩增第一个ds-cDNA以形成第一个扩增的RNA（aRNA）; 用已知序列标记第一个aRNA的3'末端以形成3'-标记的第一个aRNA; 复制3'标记的第一个aRNA以形成第二个ss-cDNA; 并使mRNA和/或第一个aRNA正常化。

2. 发明授权

US06582936B1 Methods for making nucleic acids 失效
标题翻译：制备核酸的方法
公开(公告)号：US06582936B1
公开(公告)日：2003-06-24
申请号：US09566570
申请日：2000-05-08
申请人： Tito Serafini , Percy Luu , John Ngai , David Lin
发明人： Tito Serafini , Percy Luu , John Ngai , David Lin
IPC分类号： C12P1934
CPC分类号： C12Q1/6853 , C12N15/1096 , C12Q1/6809 , C12Q2531/143 , C12Q2525/173 , C12Q2525/125 , C12Q2525/107 , C12Q2525/191
摘要： Nucleic acids are made by converting a primed single-stranded DNA to a double-stranded DNA by a method comprising the step contacting the single-stranded DNA with a DNA polymerase having 5′ exonuclease activity under conditions whereby the DNA polymerase converts the-single stranded DNA to the double-stranded DNA, wherein the single-stranded DNA is primed with oligonucleotide primer comprising a sequence complementary to the 3′ end of the single-stranded DNA, and at least one of the 5′ end of the primer and the single-stranded DNA comprises an RNA polymerase promoter joined to an upstream (5′) flanking moiety which protects the promoter from the 5′ exonuclease activity of the DNA polymerase.
摘要翻译：通过包括使单链DNA与具有5'外切核酸酶活性的DNA聚合酶接触的步骤的方法将引发的单链DNA转化为双链DNA来制备核酸，由此DNA聚合酶将单链DNA转化为单链DNA DNA到双链DNA，其中单链DNA用包含与单链DNA的3'末端互补的序列的寡核苷酸引物引物，并且引物的5'末端和单个DNA中的至少一个标记的DNA包含连接到上游（5'）侧翼部分的RNA聚合酶启动子，其保护启动子不受DNA聚合酶的5'核酸外切酶活性的影响。

3. 发明授权

US06441269B1 Methods for defining cell types 失效
标题翻译：用于定义单元格类型的方法
公开(公告)号：US06441269B1
公开(公告)日：2002-08-27
申请号：US09567637
申请日：2000-05-09
申请人： Tito Serafini , John Ngai
发明人： Tito Serafini , John Ngai
IPC分类号： C12N1500
CPC分类号： C12N15/1096 , C12N2517/02 , C12Q1/6809 , C12Q1/6855 , C12Q1/6865 , C12Q1/6897 , C12Q2565/518 , C12Q2531/143 , C12Q2525/191
摘要： The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type
摘要翻译：本发明提供了用于定义细胞类型的方法和组合物，通常涉及以下步骤：（a）扩增异源细胞群体的单细胞的mRNA; （b）探测具有扩增的mRNA的综合表达文库以定义细胞的总表达谱; 和（c）将细胞的总表达谱与一个或多个其它细胞的总表达谱进行比较以定义细胞的唯一表达谱，其中细胞的唯一表达谱提供定义细胞类型的标记

4. 发明授权

US06410249B1 Odorant receptors 失效
标题翻译：气味受体
公开(公告)号：US06410249B1
公开(公告)日：2002-06-25
申请号：US09619353
申请日：2000-07-19
申请人： John Ngai , David J. Speca , David M. Lin , Ehud Y. Isacoff , Andrew H. Dittman , Jinhong Fan
发明人： John Ngai , David J. Speca , David M. Lin , Ehud Y. Isacoff , Andrew H. Dittman , Jinhong Fan
IPC分类号： G01N33566
CPC分类号： C07K14/705 , A61K38/00 , G01N2333/726
摘要： The invention provides methods and compositions relating to odorant receptors, including a general expression cloning methodology which is useful for identifying novel G protein-coupled receptors and a novel family of odorant receptors and related nucleic acids, ligands, agonists and antagonists. These compositions provide a wide variety of applications such as screening for related receptors, and by modulating the function of the disclosed receptors by modulating their expression or contacting them with agonists, antagonist or ligands modulating reproductive/sexual and non-sexual social behaviors mediated via the olfactory system, reproductive physiologies and olfactory system regulated feeding behaviors, migratory behaviors and events such as conception, implantation, estrous and menstruation.
摘要翻译：本发明提供了与加味剂受体相关的方法和组合物，包括一般表达克隆方法，其可用于鉴定新型G蛋白偶联受体和新颖的加味剂受体和相关核酸家族，配体，激动剂和拮抗剂。这些组合物提供了各种各样的应用，例如筛选相关受体，并通过调节其所表达的受体的功能或通过调节其表达或与激动剂，拮抗剂或调节生殖/性和非性社交行为调节的配体嗅觉系统，生殖生理和嗅觉系统调节饲养行为，迁移行为和受孕，植入，发情和月经等事件。

5. 发明授权

US08497721B1 Pass gate shadow latch 有权
标题翻译：通过门阴影锁
公开(公告)号：US08497721B1
公开(公告)日：2013-07-30
申请号：US13160569
申请日：2011-06-15
申请人： Hamid Partovi , Alfred Yeung , John Ngai , Ronen Cohen
发明人： Hamid Partovi , Alfred Yeung , John Ngai , Ronen Cohen
IPC分类号： H03K3/289
CPC分类号： H03K3/356156
摘要： A latch device is provided with a relay and a shadow latch. The relay has an input to accept a binary relay input signal, an input to accept a clock signal, an input to accept a shadow-Q signal, and an output to supply a binary Q signal value equal to the relay input signal value. The relay output is supplied in response to the relay input signal, the shadow-Q signal, and the clock signal. The shadow latch has an input to accept the relay input signal, an input to accept the clock signal, and an output to supply the shadow-Q signal with a value equal to an inverted Q signal value. The shadow latch output is supplied in response to the relay input signal and clock signal.
摘要翻译：闩锁装置设置有继电器和阴影闩锁。继电器具有接受二进制继电器输入信号的输入端，接收时钟信号的输入端，接收阴影Q信号的输入端和提供等于继电器输入信号值的二进制Q信号值的输出端。响应于继电器输入信号，阴影-Q信号和时钟信号提供继电器输出。阴影锁存器具有用于接受继电器输入信号的输入端，用于接受时钟信号的输入端，以及用于向shadow-Q信号提供等于反相Q信号值的值的输出端。响应于继电器输入信号和时钟信号提供阴影锁存输出。

6. 发明授权

US08421514B1 Hazard-free minimal-latency flip-flop (HFML-FF) 有权
标题翻译：无危险的最小延迟触发器（HFML-FF）
公开(公告)号：US08421514B1
公开(公告)日：2013-04-16
申请号：US13225044
申请日：2011-09-02
申请人： Alfred Yeung , Hamid Partovi , Luca Ravezzi , John Ngai
发明人： Alfred Yeung , Hamid Partovi , Luca Ravezzi , John Ngai
IPC分类号： H03K3/289
CPC分类号： H03K3/013 , H03K3/356156 , H03K3/35625
摘要： A hazard-free minimal-latency flip-flop (HFML-FF) is provided. A master latch includes an input to accept a D1 signal, an input to accept a clock signal, an input to accept an inverted shadow-D2 signal, and an output to supply a D2 signal. The master latch has an input to accept a shadow-D1 signal, an input to accept the clock signal, and an output to supply a shadow-D2 signal and the inverted shadow-D2 signal. The slave latch has an input to accept the D2 signal, an input to accept the clock signal, an input to accept an inverted shadow-Q signal, and an output to supply a Q signal. The slave latch has an input to accept either the D2 signal or the shadow-D2 signal, an input to accept the clock signal, and an output to supply a shadow-Q signal and the inverted shadow-Q signal. The design may use clocked inverters or pass gates.
摘要翻译：提供无危险的最小延迟触发器（HFML-FF）。主锁存器包括接收D1信号的输入端，接受时钟信号的输入端，接收反相阴影D2信号的输入端和提供D2信号的输出端。主锁存器具有接受阴影D1信号的输入端，接受时钟信号的输入端，以及提供阴影D2信号和反相阴影D2信号的输出端。从锁存器具有接收D2信号的输入端，接受时钟信号的输入端，接收反相阴影Q信号的输入端和提供Q信号的输出端。从锁存器具有接收D2信号或阴影D2信号的输入端，用于接受时钟信号的输入端和用于提供阴影Q信号和反相阴影Q信号的输出。该设计可以使用时钟反相器或通过门。

7. 发明授权

US6114152A Methods for making nucleic acids 失效
标题翻译：制备核酸的方法
公开(公告)号：US6114152A
公开(公告)日：2000-09-05
申请号：US49806
申请日：1998-03-27
申请人： Tito Serafini , Percy Luu , John Ngai , David Lin
发明人： Tito Serafini , Percy Luu , John Ngai , David Lin
IPC分类号： C12N15/09 , C12N15/10 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6809 , C12N15/1096 , C12Q1/6853
摘要： Nucleic acids are made by adding a known nucleotide sequence to the 3' end of a first RNA having a known sequence at the 5' end to form a second RNA and reverse transcribing the second RNA to form a cDNA. In one embodiment, the first RNA is an amplified mRNA, the known sequence at the 5' end comprises a poly(T) sequence, the adding step comprises using a polyadenyltransferase to add a poly(A) sequence to the 3' end, the reverse transcribing step is initiated at a duplex region comprising the poly(T) sequence hybridized to the poly(A) sequence, the cDNA is converted to double-stranded cDNA by a polymerase initiating from a noncovalently joined duplex region, and the double-stranded cDNA is transcribed to form one or more third RNAs.
摘要翻译：通过向5'末端具有已知序列的第一个RNA的3'末端添加已知的核苷酸序列以形成第二个RNA并逆转录录第二个RNA以形成cDNA来制备核酸。在一个实施方案中，第一RNA是扩增的mRNA，5'末端的已知序列包含poly（T）序列，所述添加步骤包括使用聚腺苷酸转移酶向3'端添加聚（A）序列，在包含与poly（A）序列杂交的poly（T）序列的双链体区域开始逆转录步骤，通过从非共价连接的双链体区域起始的聚合酶将cDNA转化为双链cDNA，双链 cDNA被转录以形成一个或多个第三RNA。

8. 发明授权

US08604854B1 Pseudo single-phase flip-flop (PSP-FF) 有权
标题翻译：伪单相触发器（PSP-FF）
公开(公告)号：US08604854B1
公开(公告)日：2013-12-10
申请号：US13412679
申请日：2012-03-06
申请人： Hamid Partovi , Alfred Yeung , Luca Ravezzi , John Ngai
发明人： Hamid Partovi , Alfred Yeung , Luca Ravezzi , John Ngai
IPC分类号： H03K3/289 , H03K3/356
CPC分类号： H03K3/356113
摘要： Disclosed herein is a pseudo single-phase flip-flop. The master section includes a pre-dissipation stage and a first keeper. The pre-dissipation stage discharges the first keeper to the mDb second binary value, and selectively charges the first keeper with the mDb first binary value in the master pass mode. The pre-dissipation stage selectively prevents the first keeper from charging to the mDb first binary value in response to one of the clock phases. The slave section includes a pre-charge stage, a second keeper, a post-dissipation stage, and a third keeper. The second keeper maintains a first binary value in a slave pass mode when the mDb signal has a second binary value. The second keeper supports the second binary value in the slave pass mode when the mDb signal has the first binary value. The third keeper maintains the Q signal binary value during the slave hold mode.
摘要翻译：这里公开了伪单相触发器。主部分包括预耗散阶段和第一保持器。预耗散级将第一保持器放电到mDb第二二进制值，并且在主通过模式中以mDb第一二进制值选择性地对第一保持器充电。响应于时钟相位之一，预耗散级选择性地防止第一保持器充电到mDb第一二进制值。从部分包括预充电阶段，第二保持器，后消耗阶段和第三保持器。当mDb信号具有第二二进制值时，第二保持器保持从通模式中的第一二进制值。当mDb信号具有第一二进制值时，第二保持器支持从通模式中的第二二进制值。第三保持器在从机保持模式期间保持Q信号二进制值。

9. 发明授权

US6110711A Method of defining cell types by probing comprehensive expression libraries with amplified RNA 失效
标题翻译：通过用扩增的RNA探测综合表达文库来定义细胞类型的方法
公开(公告)号：US6110711A
公开(公告)日：2000-08-29
申请号：US212338
申请日：1998-12-15
申请人： Tito Serafini , John Ngai
发明人： Tito Serafini , John Ngai
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12N15/1096 , C12Q1/6809 , C12Q1/6855 , C12Q1/6865 , C12N2517/02 , C12Q1/6897
摘要： The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type.
摘要翻译：本发明提供了用于定义细胞类型的方法和组合物，通常涉及以下步骤：（a）扩增异源细胞群体的单细胞的mRNA; （b）探测具有扩增的mRNA的综合表达文库以定义细胞的总表达谱; 和（c）将细胞的总表达谱与一个或多个其它细胞的总表达谱进行比较以限定细胞的唯一表达谱，其中细胞的唯一表达谱提供定义细胞类型的标记。

10. 发明授权

US08618856B1 Shadow latch 有权
标题翻译：阴影闩锁
公开(公告)号：US08618856B1
公开(公告)日：2013-12-31
申请号：US13077949
申请日：2011-03-31
申请人： Alfred Yeung , Hamid Partovi , John Ngai , Ronen Cohen
发明人： Alfred Yeung , Hamid Partovi , John Ngai , Ronen Cohen
IPC分类号： H03K3/289
CPC分类号： H03K3/013 , H03K3/356121
摘要： A latch device is provided with a driver and a shadow latch. The driver has an input to accept a binary driver input signal, an input to accept a clock signal, and an input to accept a shadow-Q signal. The driver has an output to supply a binary Q signal equal to the inverse of the driver input signal, in response to the driver input signal, the shadow-Q signal, and the clock signal. The shadow latch has an input to accept the driver input signal, and an input to accept the clock signal. The shadow latch has an output to supply the shadow-Q signal equal to the inverted Q signal, in response to the driver input signal and clock signal.
摘要翻译：闩锁装置设置有驱动器和阴影闩锁。驱动器具有接受二进制驱动器输入信号的输入端，接受时钟信号的输入端和接收阴影Q信号的输入端。驱动器具有响应于驱动器输入信号，阴影-Q信号和时钟信号而提供等于驱动器输入信号的倒数的二进制Q信号的输出。阴影锁存器具有接受驱动器输入信号的输入端和接受时钟信号的输入端。阴影锁存器具有响应于驱动器输入信号和时钟信号而提供等于反相Q信号的阴影Q信号的输出。

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