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    • 5. 发明授权
    • Method of fluorometrically measuring the activity of fat-degrading
enzymes
    • 荧光测量脂肪降解酶活性的方法
    • US4668623A
    • 1987-05-26
    • US582527
    • 1984-02-22
    • Paavo K. J. KinnunenTom M. SchroderJorma A. Virtanen
    • Paavo K. J. KinnunenTom M. SchroderJorma A. Virtanen
    • C12Q1/34C12Q1/61C12Q1/44
    • C12Q1/34C12Q1/61C12Q2334/00G01N2333/918
    • The object of the present invention is a new method for fluorometrically assaying the activity of fat-degrading enzymes, such as lipases and phospholipases in samples containing said enzyme, such as in serum. According to the method the enzyme containing sample is reacted with a substrate containing acyl- or alkylglycerols or -phosphoglycerols having at least one fluorescent group, such as a pyrene group. The compounds may in addition contain flurorescence quenching groups. The enzyme hydrolyzes the substrate thus giving rise to changes in the fluorescence intensity during the enzyme reaction and the changes in the intensity are measured at a specific emission wavelength of the fluorescent group employed. The rate of change of the invensity is proportional to the enzyme activity in the sample.The invention concerns also the new compounds containing fluorescent groups to be used in the method.
    • 本发明的目的是在含有所述酶的样品例如血清中荧光测定脂肪降解酶如脂肪酶和磷脂酶的活性的新方法。 根据该方法,含有酶的样品与含有具有至少一个荧光基团如芘基团的酰基 - 或烷基甘油或 - 磷酸甘油的底物反应。 该化合物可另外含有花青猝灭基团。 酶水解底物,从而在酶反应期间引起荧光强度的变化,并且在所用荧光基团的特定发射波长处测量强度的变化。 其强度变化率与样品中的酶活性成正比。 本发明还涉及用于该方法的含有荧光基团的新化合物。
    • 6. 发明授权
    • Process for preparing 1,2-diacyl-sn-glycerols
    • 1,2-二酰基-sn-甘油的制备方法
    • US4360694A
    • 1982-11-23
    • US251364
    • 1981-04-06
    • Paavo K. J. KinnunenTom M. SchroderJorma A. Virtanen
    • Paavo K. J. KinnunenTom M. SchroderJorma A. Virtanen
    • C07C69/003C07F5/02C09F5/08C07C67/02C11C3/02
    • C07F5/025Y02P20/55
    • The object of the present invention is a novel and simple process for the production of 1,2-diacyl-sn-glycerols, wherein the acyl groups may be the same or different. According to the invention D-mannitol is reacted with benzene boronic acid or a derivative thereof to form a D-mannitol-monobenzeneboronate which compound is acylated either to form, after removal of the benzeneboronate protective group, a 1,2,5,6-tetraacyl-D-mannitol with four identical acyl groups, or is acylated selectively in two stages, by first acylating the 1,6-hydroxy groups of the boronate protected D-mannitol to form, after removal of the boronate protection, a 1,6-diacyl-D-mannitol, which then is repeatedly reacted with a benzene boronic acid or a derivative thereof and then acylated in the 2- and 5-positions. After removal of the boronate protective group, the tetra-acylated D-mannitol obtained either in the one-stage or two-stage process is split by oxidation to form a 1,2-diacyl-glyceraldehyde which in turn is reduced to the corresponding 1,2-diacyl-sn-glycerol. By means of the invention it is thus possible to prepare 1,2-diacyl-sn-glycerols in which the acyl groups can be the same or different.
    • 本发明的目的是制备1,2-二酰基-sn-甘油的新颖且简单的方法,其中酰基可以相同或不同。 根据本发明,D-甘露醇与苯硼酸或其衍生物反应形成D-甘露醇 - 单苯硼酸盐,该化合物在除去苯硼酸盐保护基后形成1,2,5,6- 具有四个相同酰基的四酰基-D-甘露糖醇,或者通过首先酰化硼酸酯保护的D-甘露糖醇的1,6-羟基,在两个阶段中选择性地酰化形成去除硼酸酯保护之后形成1,6 - 二酰基-D-甘露醇,然后与苯硼酸或其衍生物反复反应,然后在2-和5-位上进行酰化。 除去硼酸酯保护基后,在一步法或两步法中得到的四酰化D-甘露糖醇通过氧化分解形成1,2-二酰基 - 甘油醛,然后将其还原成相应的1 ,2-二酰基-sn-甘油。 通过本发明,可以制备其中酰基可以相同或不同的1,2-二酰基-sn-甘油。