会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 3. 发明授权
    • Natural rubber product from fig tree and production method thereof
    • 无花果树天然橡胶制品及其生产方法
    • US06235860B1
    • 2001-05-22
    • US09322639
    • 1999-05-28
    • Hun-Seung KangKyung-Hwan Han
    • Hun-Seung KangKyung-Hwan Han
    • C08G8300
    • C08C1/00Y10S528/931
    • Natural rubber products are made from fig tree (Ficus carica) by homogenizing the plants in an acetone/benzene and separating the rubber-containing phase by centrifugation. F. carica contains about 4% (w/v) natural rubber in latex, and 0.3, 0.1 and 0.1 % in bark, leaf and fruit, respectively. Molecular mass of the natural rubber from F. carica is about 128 kD. The latex serum of F. carica contains only a small numbers of proteins, with a 48 kD and a 25 kD protein as major proteins. Similar to Hevea brasiliensis and Parthenium argentatum Gray, proteins participating in rubber biosynthesis of F. carica are tightly associated with the rubber particles. Natural rubber is made using the latex serum and the proteins in latex, leaf, bark, and fruit of F. carica.
    • 天然橡胶制品由无花果树(Ficus carica)制成,通过在丙酮/苯中均匀化植物,并通过离心分离含橡胶的相。 car ica分别含有胶乳中约4%(w / v)的天然橡胶,分别含有0.3,0.1和0.1%的树皮,叶子和水果。 来自樟树的天然橡胶的分子质量约为128kD。 F.carica的乳胶血清只含有少量蛋白质,以48kD和25kD蛋白为主要蛋白质。 类似于三叶草(Brassiliensis)和金缕梅(Gray),参与橡胶生物合成的蛋白质与橡胶颗粒紧密相关。 天然橡胶使用胶乳血清和乳胶,叶,树皮和果实中的蛋白质制成。
    • 8. 发明授权
    • Genetic transformation of orchids
    • 兰花遗传转化
    • US6020538A
    • 2000-02-01
    • US71204
    • 1998-05-01
    • Kyung-Hwan HanJaemo Yang
    • Kyung-Hwan HanJaemo Yang
    • C12N15/31C12N15/82C12N15/54C12N15/65
    • C12N15/8207
    • A method is disclosed for making genetically engineered orchid species using accelerated particle transformation. Meristem-tip tissue of orchid is cultured into a protocorm-like body (PLB). PLB is meristematic tissue and can be induced to regenerate into whole plant. In order to achieve efficient transformation it is necessary that relatively large numbers of meristematic cells are bombarded with the DNA-coated particles. After a particle-mediated transformation event, it is also important to stimulate continued meristemoid development before and during antibiotic selection and to reduce toxic effect of oxidized products of phenolics exuded after the wounding. It has been discovered that by using the techniques described in the present invention the meristematic tissue development, before and after the bombardment, can be stimulated while the toxic effect of oxidized phenolics can be reduced, resulting in successful transformation. The bombarded PLBs are multiplied and selected for the presence of gene products coded by the introduced genes. Shoot regeneration is then induced from the PLBs and transgenic seedlings are produced. Preferred plants for carrying out the present invention are the members of the genus Cymbidium.
    • 公开了一种使用加速粒子转化来制造转基因植物兰花物种的方法。 兰花的分生组织培养成原生质体样体(PLB)。 PLB是分生组织,可诱导再生成全植株。 为了实现有效的转化,需要用DNA涂覆的颗粒轰击相对大量的分生细胞。 在粒子介导的转化事件之后,在抗生素选择之前和期间刺激持续的分生组织发育以及减少伤口后渗出的酚类氧化产物的毒性作用也是重要的。 已经发现,通过使用本发明中描述的技术,可以刺激在轰击之前和之后的分生组织发育,同时可以减少氧化酚类的毒性作用,导致成功的转化。 将被轰击的PLB乘以并选择由引入的基因编码的基因产物的存在。 然后从PLB诱导射击再生,并产生转基因幼苗。 用于实施本发明的优选植物是蕙兰属的成员。