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1. 发明授权

US08029988B2 Oligonucleotide mediated nucleic acid recombination 有权
公开(公告)号：US08029988B2
公开(公告)日：2011-10-04
申请号：US11987555
申请日：2007-11-30
申请人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P. C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , Jon E. Ness , Claes Gustafsson , Phillip A. Patten
IPC分类号： C12Q1/68 , C12N15/00 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12P21/06
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

2. 发明授权

US07826597B2 Methods and apparatus to handle customer support requests 有权
标题翻译：处理客户支持请求的方法和设备
公开(公告)号：US07826597B2
公开(公告)日：2010-11-02
申请号：US11298001
申请日：2005-12-09
申请人： Earl A. Berner , Kristina Robinson , Mark Magnusson , Jason Porter , James Rushing , Mark Welch
发明人： Earl A. Berner , Kristina Robinson , Mark Magnusson , Jason Porter , James Rushing , Mark Welch
IPC分类号： H04M1/24 , H04M3/08
CPC分类号： H04M3/5166 , H04M3/10 , H04M2203/2011
摘要： Methods and apparatus to handle support contacts in a call center are disclosed. An example method for handling a first contact to a support center includes receiving the first contact from a first person, receiving a first identifier associated with the first person, analyzing contact details for a second contact associated with a second identifier associated with a second person different from the first person to determine if a problem associated with the first identifier exists, and indicating that a problem associated with the first identifier exists if the contact details for the second contact associated with the second identifier indicate that a problem associated with the first identifier exists.
摘要翻译：公开了一种在呼叫中心处理支持联系人的方法和装置。用于处理到支持中心的第一联系人的示例性方法包括从第一人接收第一联系人，接收与第一人相关联的第一标识符，分析与第二人相关联的第二联系人的联系人细节，所述第二标识符与第二人不相关从第一人员确定是否存在与第一标识符相关联的问题，并且如果与第二标识符相关联的第二联系人的联系人详细信息指示存在与第一标识符相关联的问题，则指示存在与第一标识符相关联的问题。

3. 发明申请

US20100105599A1 SUBTILISIN VARIANTS 审中-公开
公开(公告)号：US20100105599A1
公开(公告)日：2010-04-29
申请号：US12562517
申请日：2009-09-18
申请人： Jon E. Ness , Mark Welch , Lorraine J. Giver , Joel R. Cherry , Torben V. Borchert , Jeremy S. Minshull
发明人： Jon E. Ness , Mark Welch , Lorraine J. Giver , Joel R. Cherry , Torben V. Borchert , Jeremy S. Minshull
IPC分类号： C12N9/56 , C11D7/42
CPC分类号： C12N9/6408 , C12N9/54
摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.
摘要翻译：提供了新的枯草杆菌蛋白酶同源物（核酸和蛋白质）。还提供了包括这些新蛋白质，重组细胞，涉及新同系物的洗牌方法，新同系物的抗体以及使用同源物的方法的组合物。

4. 发明申请

US20100029493A1 DESIGN OF SYNTHETIC NUCLEIC ACIDS FOR EXPRESSION OF ENCODED PROTEINS 有权
标题翻译：用于表达编码蛋白的合成核酸的设计
公开(公告)号：US20100029493A1
公开(公告)日：2010-02-04
申请号：US12184230
申请日：2008-07-31
申请人： Mark Welch , Claes Gustafsson
发明人： Mark Welch , Claes Gustafsson
IPC分类号： C40B30/02 , C40B60/12
CPC分类号： G06F19/24 , G06F19/20
摘要： A method of determining a property that affects expression of polynucleotides in an expression system. A plurality of polynucleotides, each encoding a polypeptide sequence, is constructed. An amino acid is encoded a plurality of times in both a first and second polynucleotide in the plurality. The amino acid is encodable by a plurality of synonymous codons including a first codon. The first codon is in the first polynucleotide at a first frequency relative to other synonymous codons, and is in the second polynucleotide at a second frequency relative to other synonymous codons. The first and second frequencies are different. Each polynucleotide is individually expressed in the expression system to measure an expression property value of the polynucleotides, thereby determining a property that affects expression of polynucleotides. The property is an effect that a frequency of use of one or more codons has on expression of polynucleotides in the expression system.
摘要翻译：确定影响表达系统中多核苷酸表达的性质的方法。构建多个编码多肽序列的多核苷酸。氨基酸在多个第一和第二多核苷酸两者中编码多次。氨基酸可由包括第一密码子的多个同义密码子编码。相对于其他同义密码子，第一个密码子以第一个频率位于第一个多核苷酸中，并且相对于其他同义密码子在第二个频率处于第二个多核苷酸。第一和第二频率是不同的。每个多核苷酸在表达系统中单独表达以测量多核苷酸的表达特性值，从而确定影响多核苷酸表达的性质。该性质是一种或多种密码子的使用频率对表达系统中多核苷酸表达的影响。

5. 发明授权

US07605115B2 Subtilisin variants 失效
标题翻译：枯草杆菌蛋白酶变体
公开(公告)号：US07605115B2
公开(公告)日：2009-10-20
申请号：US11736322
申请日：2007-04-17
申请人： Jon E. Ness , Mark Welch , Lorraine J. Giver , Joel R. Cherry , Torben V. Borchert , Jeremy Minshull
发明人： Jon E. Ness , Mark Welch , Lorraine J. Giver , Joel R. Cherry , Torben V. Borchert , Jeremy Minshull
IPC分类号： C12N9/50 , C12N9/54 , C12N15/57 , C12N15/74 , C11D3/386
CPC分类号： C12N9/6408 , C12N9/54
摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.
摘要翻译：提供了新的枯草杆菌蛋白酶同源物（核酸和蛋白质）。还提供了包括这些新蛋白质，重组细胞，涉及新同系物的洗牌方法，新同系物的抗体以及使用同源物的方法的组合物。

6. 发明申请

US20090183743A1 CIGAR PUNCTURE TOOL WITH CYLINDRICAL BODY AND INNER SPIKE 审中-公开
标题翻译： CIGAR PUNCTURE工具与圆柱体和内部的SPIKE
公开(公告)号：US20090183743A1
公开(公告)日：2009-07-23
申请号：US12016691
申请日：2008-01-18
申请人： Randy Simmons , Mark Welch
发明人： Randy Simmons , Mark Welch
IPC分类号： A24F13/24
CPC分类号： A24F13/26
摘要： A generally cylindrical shaped cigar puncture tool incorporating a spring loaded and locating/guiding disk element arranged in conjunction with a linearly extending and base supported spike. Centered puncturing of the closed head occurs in a first downward translating direction of the cigar within the open interior of the tool. A return spring bias causing even disengaging of the cigar in a second reverse direction. The sizing of an aperture in the disk further allows passage therethrough of the spike, while preventing debris from the cigar head cluttering the puncture zone within the tool.
摘要翻译：一种通常为圆柱形的雪茄穿刺工具，其结合弹性加载和定位/引导盘元件，其与线性延伸和底座支撑的尖钉结合设置。闭合头部的中心穿刺在工具的开放内部的雪茄的第一向下平移方向上发生。复位弹簧偏置使得雪茄在第二相反方向上甚至脱离。盘中的孔径的尺寸进一步允许尖头通过，同时防止来自雪茄头的碎屑使穿刺区域在工具内部杂乱。

7. 发明授权

US07561972B1 Synthetic nucleic acids for expression of encoded proteins 有权
标题翻译：用于表达编码蛋白质的合成核酸
公开(公告)号：US07561972B1
公开(公告)日：2009-07-14
申请号：US12184234
申请日：2008-07-31
申请人： Mark Welch , Claes Gustafsson
发明人： Mark Welch , Claes Gustafsson
IPC分类号： G01N33/48 , C12Q1/00 , C12Q1/48 , C07H21/02
CPC分类号： C12N15/67 , G06F19/20 , G06F19/22
摘要： A method of designing a polynucleotide sequence encoding a polypeptide sequence of a predetermined polypeptide is provided. A frequency lookup table corresponding to an expression system is obtained. The table comprises a plurality of sequence elements and a plurality of frequency ranges, each frequency range for a corresponding sequence element. Each frequency range is a range of frequencies with which a corresponding sequence element can occur in a polynucleotide. The polynucleotide sequence is defined using the frequency lookup table by determining, for each respective sequence element in the frequency lookup table, whether the respective sequence element encodes a portion of the polypeptide sequence. When the respective sequence element encodes a portion of the polypeptide sequence, the sequence element is incorporated into the polynucleotide at a frequency of occurrence that is within the frequency range specified for the respective sequence element in the lookup table. The polynucleotide sequence is then outputted.
摘要翻译：提供了设计编码预定多肽的多肽序列的多核苷酸序列的方法。获得与表达式系统对应的频率查找表。该表包括多个序列元素和多个频率范围，每个频率范围用于相应的序列元素。每个频率范围是在多核苷酸中可以发生相应序列元件的频率范围。使用频率查找表来定义多核苷酸序列，对于频率查找表中的每个相应序列元件，确定各个序列元件是否编码多肽序列的一部分。当各个序列元件编码多肽序列的一部分时，序列元件以在查找表中对于相应序列元件指定的频率范围内的发生频率被并入多核苷酸。然后输出多核苷酸序列。

8. 发明申请

US20070133780A1 Methods and apparatus to handle customer support requests 有权
标题翻译：处理客户支持请求的方法和设备
公开(公告)号：US20070133780A1
公开(公告)日：2007-06-14
申请号：US11298001
申请日：2005-12-09
申请人： Earl Berner , Kristina Robinson , Mark Magnusson , Jason Porter , James Rushing , Mark Welch
发明人： Earl Berner , Kristina Robinson , Mark Magnusson , Jason Porter , James Rushing , Mark Welch
IPC分类号： H04M3/00 , H04M5/00
CPC分类号： H04M3/5166 , H04M3/10 , H04M2203/2011
摘要： Methods and apparatus to handle support contacts in a call center are disclosed. An example method for handling a first contact to a support center includes receiving the first contact from a first person, receiving a first identifier associated with the first person, analyzing contact details for a second contact associated with a second identifier associated with a second person different from the first person to determine if a problem associated with the first identifier exists, and indicating that a problem associated with the first identifier exists if the contact details for the second contact associated with the second identifier indicate that a problem associated with the first identifier exists.
摘要翻译：公开了一种在呼叫中心处理支持联系人的方法和装置。用于处理到支持中心的第一联系人的示例性方法包括从第一人接收第一联系人，接收与第一人相关联的第一标识符，分析与第二人相关联的第二联系人的联系人细节，所述第二标识符与第二人不相关从第一人员确定是否存在与第一标识符相关联的问题，并且如果与第二标识符相关联的第二联系人的联系人详细信息指示存在与第一标识符相关联的问题，则指示存在与第一标识符相关联的问题。

9. 发明授权

US08401798B2 Systems and methods for constructing frequency lookup tables for expression systems 有权
标题翻译：用于构建表达系统的频率查找表的系统和方法
公开(公告)号：US08401798B2
公开(公告)日：2013-03-19
申请号：US12184233
申请日：2008-07-31
申请人： Mark Welch , Claes Gustafsson
发明人： Mark Welch , Claes Gustafsson
IPC分类号： G01N33/34 , G06F19/00
CPC分类号： C12Q1/68 , G06F19/20 , G06F19/22 , G06F19/24
摘要： Methods for determining a property that affects expression of polynucleotides are provided. A plurality of polynucleotides each encoding a polypeptide sequence is constructed. A frequency that a sequence element is used in a first polynucleotide is different than in a second polynucleotide. Each polynucleotide is expressed in an expression system to obtain an expression property value thereby constructing a dataset that contains, for each respective polynucleotide, sequence element occurrence in the respective polynucleotide and the measured expression property value of the respective polynucleotide. A model is computed that describes variation in the measured expression property values as a function of a plurality of variables and weights. From the model, a property that affects expression of polynucleotides in the expression system is determined, where the property is an effect that the frequency of occurrence of one or more sequence elements has on the expression property of polynucleotides in the expression system.
摘要翻译：提供了确定影响多核苷酸表达的性质的方法。构建多个编码多肽序列的多核苷酸。在第一多核苷酸中使用序列元件的频率与第二多核苷酸不同。每个多核苷酸在表达系统中表达以获得表达属性值，由此构建对于各个多核苷酸包含各个多核苷酸中的序列元件出现和各个多核苷酸的测量的表达特性值的数据集。计算描述作为多个变量和权重的函数的测量表达式属性值的变化的模型。从该模型中，确定影响表达系统中多核苷酸表达的性质，其中性质是一种或多种序列元件的出现频率对表达系统中多核苷酸的表达性质的影响。

10. 发明申请

US20100184627A1 OLIGONUCLEOTIDE MEDIATED NUCLEIC ACID RECOMBINATION 有权
标题翻译：寡核苷酸介导的核酸重组
公开(公告)号：US20100184627A1
公开(公告)日：2010-07-22
申请号：US12557829
申请日：2009-09-11
申请人： Andreas Crameri , Willem P.C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , John E. Ness , Claes Gustafsson , Phillip A. Patten
发明人： Andreas Crameri , Willem P.C. Stemmer , Jeremy Minshull , Steven H. Bass , Mark Welch , John E. Ness , Claes Gustafsson , Phillip A. Patten
IPC分类号： C40B50/00
CPC分类号： C12P21/06
摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
摘要翻译：提供重组核酸的方法，包括同源核酸。还提供了基因改组寡核苷酸家族及其在重组过程中的应用，以及聚合酶和连接酶介导的重组方法。

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