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    • 7. 发明申请
    • ENVIRONMENTAL STRESS RESPONSIVE PROMOTER AND METHOD OF TISSUE-SPECIFIC GENE EXPRESSION USING THE SAME
    • 环境应激反应促进剂及使用其组织特异性基因表达的方法
    • US20090282582A1
    • 2009-11-12
    • US12311900
    • 2007-10-19
    • Motoaki SekiJong-Myong KimKazuo ShinozakiMiki Fujita
    • Motoaki SekiJong-Myong KimKazuo ShinozakiMiki Fujita
    • C12N15/82C07H21/04C12N15/63A01H5/00C12N5/10
    • C07K14/415C12N15/8237
    • This invention concerns induction of gene expression specifically in given tissue with the use of a novel promoter that has environmental stress responsiveness and functions specifically in given tissue. It is realized by a method comprising: a step of preparing a plant having an arbitrary gene downstream of the environmental stress responsive promoter comprising DNA (a), (b), or (c) below; (a) DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8; (b) DNA comprising a nucleotide sequence derived from the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 by deletion, substitution, or addition of one or a plurality of nucleotides and functioning as an environmental stress responsive promoter; or (c) DNA hybridizing under stringent conditions to DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 and functioning as an environmental stress responsive promoter; and a step of cultivating the plant in the presence of environmental stress, the method comprising inducing expression of the gene located downstream of the environmental stress responsive promoter in a tissue-specific manner.
    • 本发明涉及使用具有环境应激反应性和特定于给定组织中功能的新型启动子来特异性诱导给定组织中的基因表达。 通过以下方法实现:包括下述DNA(a),(b)或(c)的环境胁迫响应启动子下游具有任意基因的植物的步骤; (a)包含SEQ ID NO:1至8中任一个所示的核苷酸序列的DNA; (b)通过缺失,取代或添加一个或多个核苷酸并起到环境应激反应启动子的作用,其包含衍生自SEQ ID NO:1至8中任一项所示核苷酸序列的核苷酸序列的DNA; 或(c)在严格条件下与包含SEQ ID NO:1至8中任一所示的核苷酸序列的DNA杂交并用作环境胁迫响应启动子的DNA的DNA; 以及在存在环境胁迫的情况下培育植物的步骤,所述方法包括以组织特异性方式诱导位于环境胁迫响应启动子下游的基因的表达。