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1. 发明授权

US08163661B2 Luminescence measurement system 有权
标题翻译：发光测量系统
公开(公告)号：US08163661B2
公开(公告)日：2012-04-24
申请号：US12725878
申请日：2010-03-17
申请人： Ryutaro Akiyoshi , Hirobumi Suzuki
发明人： Ryutaro Akiyoshi , Hirobumi Suzuki
IPC分类号： G01N21/00
CPC分类号： G01N21/763 , Y10T442/188
摘要： Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample.
摘要翻译：公开了一种发光测定方法，其即使在生物样品中以等于或大于给定量的量发生物质时，也可以根据待测物质的量产生发光强度，并且可以实现定量测量。该方法的特征在于包括制备含有发光相关蛋白的生物样品，其能够以等于或大于给定量的量与生物样品中发生的物质反应，并且其Km值等于或高于使得可以根据物质的量量测量发光强度，测量从生物样品发射的发光强度，并将该测量结果输出到生物样品的区域和/或部分上。

2. 发明申请

US20100219353A1 APPARATUS AND METHOD OF ACQUIRING IMAGES OF A SAMPLE OF BIOLOGICAL ORIGIN 审中-公开
标题翻译：获取生物原始样本图像的装置和方法
公开(公告)号：US20100219353A1
公开(公告)日：2010-09-02
申请号：US12159357
申请日：2006-12-26
申请人： Ryutaro Akiyoshi , Hirobumi Suzuki , Morinao Fukuoka
发明人： Ryutaro Akiyoshi , Hirobumi Suzuki , Morinao Fukuoka
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G02B21/0088
摘要： A novel apparatus and a novel method of imaging a luminescence phenomenon of a sample of biological origin using an optical image formation device are provided. In the inventive method and apparatus, an illumination image acquired by illuminating the sample and a luminescence image acquired with the light emitted by a luminescence phenomenon of a cell in the sample without illuminating it are superposed to generate a superposition image. In the superposition image, an analysis region is determined. Thereby, even when the light detected in a luminescence observation is feeble, a luminescent cell and/or a luminescent site in a cell in a sample can be specified.
摘要翻译：提供了使用光学图像形成装置对生物来源的样品的发光现象进行成像的新型装置和新颖方法。在本发明的方法和装置中，通过照射样品获取的照明图像和由样品中的单元的发光现象发射的光所获得的发光图像而不照射样品，被叠加以产生叠加图像。在叠加图像中，确定分析区域。因此，即使在发光观察中检测到的光是微弱的，也可以规定样品中的细胞中的发光单元和/或发光部位。

3. 发明授权

US08722376B2 Firefly luciferase 有权
标题翻译：萤火虫荧光素酶
公开(公告)号：US08722376B2
公开(公告)日：2014-05-13
申请号：US13589453
申请日：2012-08-20
申请人： Katsunori Ogo , Ryutaro Akiyoshi , Hirobumi Suzuki
发明人： Katsunori Ogo , Ryutaro Akiyoshi , Hirobumi Suzuki
IPC分类号： C12N9/02 , C12N15/53
CPC分类号： G01N33/535 , C12N9/0069 , G01N2333/90241
摘要： According to one embodiment, the present invention relates to luciferase derived from Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 580 nm at pH 8, or the luciferase indicating 23.3 times or more of luminescent intensity in comparison to that of Rhodamine 6G.
摘要翻译：根据一个实施方案，本发明涉及来自马来西亚Luciola萤火虫的荧光素酶，荧光素酶在pH8时具有580nm的最大发光波长，或者荧光素酶与罗丹明6G相比显示出23.3倍或更多的发光强度。

4. 发明申请

US20130040326A1 FIREFLY LUCIFERASE 有权
标题翻译：快速脱毛
公开(公告)号：US20130040326A1
公开(公告)日：2013-02-14
申请号：US13589453
申请日：2012-08-20
申请人： Katsunori OGO , Ryutaro AKIYOSHI , Hirobumi SUZUKI
发明人： Katsunori OGO , Ryutaro AKIYOSHI , Hirobumi SUZUKI
IPC分类号： C12N9/02 , C12N15/53 , G01N21/76
CPC分类号： G01N33/535 , C12N9/0069 , G01N2333/90241
摘要： According to one embodiment, the present invention relates to luciferase derived from Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 580 nm at pH 8, or the luciferase indicating 23.3 times or more of luminescent intensity in comparison to that of Rhodamine 6G.
摘要翻译：根据一个实施方案，本发明涉及来自马来西亚Luciola萤火虫的荧光素酶，荧光素酶在pH8时具有580nm的最大发光波长，或者荧光素酶与罗丹明6G相比显示出23.3倍或更多的发光强度。

5. 发明申请

US20120270247A1 LUMINESCENCE MEASUREMENT METHOD AND LUMINESCENCE MEASUREMENT SYSTEM 审中-公开
标题翻译：发光测量方法和发光测量系统
公开(公告)号：US20120270247A1
公开(公告)日：2012-10-25
申请号：US13422427
申请日：2012-03-16
申请人： Ryutaro AKIYOSHI , Hirobumi SUZUKI
发明人： Ryutaro AKIYOSHI , Hirobumi SUZUKI
IPC分类号： G01N21/76
CPC分类号： G01N21/763 , Y10T442/188
摘要： Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample.
摘要翻译：公开了一种发光测定方法，其即使在生物样品中以等于或大于给定量的量发生物质时，也可以根据待测物质的量产生发光强度，并且可以实现定量测量。该方法的特征在于包括制备含有发光相关蛋白的生物样品，其能够以等于或大于给定量的量与生物样品中发生的物质反应，并且其Km值等于或高于使得可以根据物质的量量测量发光强度，测量从生物样品发射的发光强度，并将该测量结果输出到生物样品的区域和/或部分上。

6. 发明申请

US20100209949A1 LUMINESCENCE MEASUREMENT METHOD AND LUMINESCENCE MEASUREMENT SYSTEM 有权
标题翻译：发光测量方法和发光测量系统
公开(公告)号：US20100209949A1
公开(公告)日：2010-08-19
申请号：US12725878
申请日：2010-03-17
申请人： Ryutaro AKIYOSHI , Hirobumi SUZUKI
发明人： Ryutaro AKIYOSHI , Hirobumi SUZUKI
IPC分类号： C12Q1/66 , C12M1/34
CPC分类号： G01N21/763 , Y10T442/188
摘要： Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample.
摘要翻译：公开了一种发光测定方法，其即使在生物样品中以等于或大于给定量的量发生物质时，也可以根据待测物质的量产生发光强度，并且可以实现定量测量。该方法的特征在于包括制备含有发光相关蛋白的生物样品，其能够以等于或大于给定量的量与生物样品中发生的物质反应，并且其Km值等于或高于使得可以根据物质的量量测量发光强度，测量从生物样品发射的发光强度，并将该测量结果输出到生物样品的区域和/或部分上。

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