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1. 发明授权

US08741611B2 Methods for improving malic acid production in filamentous fungi 失效
公开(公告)号：US08741611B2
公开(公告)日：2014-06-03
申请号：US12870523
申请日：2010-08-27
申请人： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
发明人： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
IPC分类号： C12P7/46 , C12N1/00
CPC分类号： C12P7/46 , C07K14/38 , C12N15/80
摘要： The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

2. 发明申请

US20120219999A1 Microorganisms for C4-Dicarboxylic Acid Production 有权
标题翻译： C4-二羧酸生产的微生物
公开(公告)号：US20120219999A1
公开(公告)日：2012-08-30
申请号：US13407584
申请日：2012-02-28
申请人： Amanda Fischer , Stephen Brown , Sheryl Luttringer
发明人： Amanda Fischer , Stephen Brown , Sheryl Luttringer
IPC分类号： C12P7/46 , C12N1/15
CPC分类号： C12P7/46 , C07K14/38 , C12N1/14
摘要： The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.
摘要翻译：本发明涉及具有碳酸氢根转运蛋白活性的分离多肽和编码多肽的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法，以及生产C4-二羧酸如苹果酸的方法。

3. 发明申请

US20110053233A1 Methods for improving malic acid production in filamentous fungi 失效
标题翻译：改善丝状真菌中苹果酸生产的方法
公开(公告)号：US20110053233A1
公开(公告)日：2011-03-03
申请号：US12870523
申请日：2010-08-27
申请人： STEPHEN BROWN , SHERYL LUTTRINGER , DEBBIE YAVER , ALAN BERRY
发明人： STEPHEN BROWN , SHERYL LUTTRINGER , DEBBIE YAVER , ALAN BERRY
IPC分类号： C12P7/46 , C12N1/15
CPC分类号： C12P7/46 , C07K14/38 , C12N15/80
摘要： The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
摘要翻译：本发明涉及生产C4二羧酸的方法，其包括：（a）培养丝状真菌宿主细胞，所述丝状真菌宿主细胞包含选自以下的多核苷酸：编码C4二羧酸转运蛋白的异源第一多核苷酸，编码苹果酸脱氢酶和编码丙酮酸羧化酶的异源第三多核苷酸; 其中当在相同条件下培养时，丝状真菌宿主细胞能够分泌与不含异源多核苷酸的丝状真菌宿主细胞相比增加的C4二羧酸水平; 和（b）回收C4二羧酸。本发明还涉及增加C4二羧酸生产，丝状真菌宿主细胞和苹果酸脱氢酶变体的方法。

4. 发明授权

US08722387B2 Microorganisms for C4-dicarboxylic acid production 有权
标题翻译：用于C4-二羧酸生产的微生物
公开(公告)号：US08722387B2
公开(公告)日：2014-05-13
申请号：US13407584
申请日：2012-02-28
申请人： Amanda Fischer , Stephen Brown , Sheryl Luttringer
发明人： Amanda Fischer , Stephen Brown , Sheryl Luttringer
IPC分类号： C12N1/00 , C12P7/46 , C12N15/00 , C07H21/02
CPC分类号： C12P7/46 , C07K14/38 , C12N1/14
摘要： The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.
摘要翻译：本发明涉及具有碳酸氢根转运蛋白活性的分离多肽和编码多肽的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法，以及生产C4-二羧酸如苹果酸的方法。

5. 发明申请

US20130059353A1 Recombinant Microorganisms for Production C4-Dicarboxylic Acids 失效
标题翻译：用于生产C4-二羧酸的重组微生物
公开(公告)号：US20130059353A1
公开(公告)日：2013-03-07
申请号：US13588322
申请日：2012-08-17
申请人： Sarah McFarland , Stephen Brown , Sheryl Luttringer
发明人： Sarah McFarland , Stephen Brown , Sheryl Luttringer
IPC分类号： C12N1/15 , C12P7/46
CPC分类号： C12N15/8243 , C12N9/88
摘要： Provided herein are host cells comprising carbonic anhydrase activity, wherein the cells are capable of producing C4-dicarboxylic acid. Also provided are methods of producing C4-dicarboxylic acid comprising (a) cultivating the host cells having carbonic anhydrase activity in a medium under suitable conditions to produce C4-dicarboxylic acid; and (b) recovering the C4-dicarboxylic acid.
摘要翻译：本文提供了包含碳酸酐酶活性的宿主细胞，其中细胞能够产生C4-二羧酸。还提供了生产C4-二羧酸的方法，其包括（a）在合适条件下培养在培养基中具有碳酸酐酶活性的宿主细胞以产生C4-二羧酸; 和（b）回收C4-二羧酸。

6. 发明授权

US08663956B2 Recombinant microorganisms for production C4-dicarboxylic acids 失效
标题翻译：用于生产C4-二羧酸的重组微生物
公开(公告)号：US08663956B2
公开(公告)日：2014-03-04
申请号：US13588322
申请日：2012-08-17
申请人： Sarah McFarland , Stephen Brown , Sheryl Luttringer
发明人： Sarah McFarland , Stephen Brown , Sheryl Luttringer
IPC分类号： C12N1/15
CPC分类号： C12N15/8243 , C12N9/88
摘要： Provided herein are host cells comprising carbonic anhydrase activity, wherein the cells are capable of producing C4-dicarboxylic acid. Also provided are methods of producing C4-dicarboxylic acid comprising (a) cultivating the host cells having carbonic anhydrase activity in a medium under suitable conditions to produce C4-dicarboxylic acid; and (b) recovering the C4-dicarboxylic acid.
摘要翻译：本文提供了包含碳酸酐酶活性的宿主细胞，其中细胞能够产生C4-二羧酸。还提供了生产C4-二羧酸的方法，其包括（a）在合适条件下培养在培养基中具有碳酸酐酶活性的宿主细胞以产生C4-二羧酸; 和（b）回收C4-二羧酸。

7. 发明授权

US08497103B2 Methods for C4-dicarboxylic acid production in filamentous fungi 失效
标题翻译：丝状真菌中C4-二羧酸生产方法
公开(公告)号：US08497103B2
公开(公告)日：2013-07-30
申请号：US13165719
申请日：2011-06-21
申请人： Sheryl Luttringer , Debbie Yaver
发明人： Sheryl Luttringer , Debbie Yaver
IPC分类号： C12N1/15 , C12P7/46
CPC分类号： C12P7/46 , C07K14/38
摘要： The present invention relates to methods of producing C4-dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4-dicarboxylic acid transporter; and (b) recovering the C4-dicarboxylic acid. The present invention also relates to methods for increasing C4-dicarboxylic acid production, as well as host cells comprising the polynucleotides.
摘要翻译：本发明涉及生产C4-二羧酸如苹果酸的方法，其包括：（a）培养包含编码C4-二羧酸转运蛋白的多核苷酸的宿主细胞; 和（b）回收C4-二羧酸。本发明还涉及增加C4-二羧酸产生的方法以及包含多核苷酸的宿主细胞。

8. 发明申请

US20110312046A1 Methods for C4 Dicarboxylic Acid Production in Filamentous Fungi 失效
标题翻译：丝状真菌中C4二羧酸生产方法
公开(公告)号：US20110312046A1
公开(公告)日：2011-12-22
申请号：US13165719
申请日：2011-06-21
申请人： SHERYL LUTTRINGER , DEBBIE YAVER
发明人： SHERYL LUTTRINGER , DEBBIE YAVER
IPC分类号： C12P7/40 , C12N1/15
CPC分类号： C12P7/46 , C07K14/38
摘要： The present invention relates to methods of producing C4-dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4-dicarboxylic acid transporter; and (b) recovering the C4-dicarboxylic acid. The present invention also relates to methods for increasing C4-dicarboxylic acid production, as well as host cells comprising the polynucleotides.
摘要翻译：本发明涉及生产C4-二羧酸如苹果酸的方法，其包括：（a）培养包含编码C4-二羧酸转运蛋白的多核苷酸的宿主细胞; 和（b）回收C4-二羧酸。本发明还涉及增加C4-二羧酸产生的方法以及包含多核苷酸的宿主细胞。

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