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1. 发明授权

US08049000B2 Early endosperm promoter eep2 失效
标题翻译：早期胚乳启动子eep2
公开(公告)号：US08049000B2
公开(公告)日：2011-11-01
申请号：US12463450
申请日：2009-05-11
申请人： Timothy G Helentjaris , Xiaomu Niu
发明人： Timothy G Helentjaris , Xiaomu Niu
IPC分类号： C12N15/29
CPC分类号： C12N15/8234 , C12N9/0032 , C12N9/1085 , C12N15/8243 , C12N15/8261 , C12N15/8271 , C12N15/8295 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes, particularly in plant seeds and related female reproductive tissue. Compositions comprise novel nucleotide sequences for a seed-preferred promoter known as eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明一般涉及植物分子生物学领域。更具体地，本发明涉及用于时间和/或空间调节基因表达的方法和试剂，特别是在植物种子和相关的雌性生殖组织中。组合物包含称为eep2的种子优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

2. 发明申请

US20110183399A1 Maize Cellulose Synthases and Uses Thereof 失效
公开(公告)号：US20110183399A1
公开(公告)日：2011-07-28
申请号：US13079071
申请日：2011-04-04
申请人： Kanwarpal S. Dhugga , Haiyin Wang , Dwight Tomes , Timothy G. Helentjaris
发明人： Kanwarpal S. Dhugga , Haiyin Wang , Dwight Tomes , Timothy G. Helentjaris
IPC分类号： C12N9/00 , A01H5/00
CPC分类号： C12N9/1059 , C07K14/415 , C12N15/8241 , C12N15/8246 , C12N15/8251 , C12N15/8261 , Y02A40/146 , Y02E50/17
摘要： The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants comprising said nucleic acids.

3. 发明申请

US20110010795A9 AP2 DOMAIN TRANSCRIPTION FACTOR ODP2 (OVULE DEVELOPMENT PROTEIN 2) AND METHODS OF USE 有权
标题翻译： AP2域转录因子ODP2（OVULE DEVELOPMENT蛋白2）及其使用方法
公开(公告)号：US20110010795A9
公开(公告)日：2011-01-13
申请号：US12503482
申请日：2009-07-15
申请人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
发明人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
IPC分类号： C12N15/82 , C12N15/11 , C12N15/00 , A01H5/00 , C12N5/04
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节植物发育的方法和组合物。提供编码Ovule Development Protein 2（ODP2）蛋白的核苷酸序列和氨基酸序列。这些序列可以用于各种方法，包括调节发育，发育途径，改变植物中的油含量，提高转化效率，调节胁迫耐受性和调节植物的再生能力。还提供了转化的植物，植物细胞，组织和种子。

4. 发明授权

US07579529B2 AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use 有权
标题翻译： AP2结构域转录因子ODP2（胚珠发育蛋白2）及其使用方法
公开(公告)号：US07579529B2
公开(公告)日：2009-08-25
申请号：US11045802
申请日：2005-01-28
申请人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
发明人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节植物发育的方法和组合物。提供编码Ovule Development Protein 2（ODP2）蛋白的核苷酸序列和氨基酸序列。这些序列可以用于各种方法，包括调节发育，发育途径，改变植物中的油含量，提高转化效率，调节胁迫耐受性和调节植物的再生能力。还提供了转化的植物，植物细胞，组织和种子。

5. 发明授权

US07531723B2 Modulation of cytokinin activity in plants 有权
标题翻译：植物中细胞分裂素活性的调节
公开(公告)号：US07531723B2
公开(公告)日：2009-05-12
申请号：US10817483
申请日：2004-04-02
申请人： Jeffrey E. Habben , Chris Zinselmeier , Dwight T. Tomes , Timothy G. Helentjaris
发明人： Jeffrey E. Habben , Chris Zinselmeier , Dwight T. Tomes , Timothy G. Helentjaris
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8234 , C12N9/0032 , C12N9/1085 , C12N15/8243 , C12N15/8261 , C12N15/8271 , C12N15/8295 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes that affect metabolically effective levels of cytokinins in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, such as improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, or stability of yield. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明一般涉及植物分子生物学领域。更具体地说，本发明涉及影响植物中特别是植物种子和相关雌性生殖组织中细胞分裂素的代谢有效水平的基因的时间和/或空间调节表达的方法和试剂。本发明进一步涉及具有增强的细胞分裂素表达水平的转基因植物，其中转基因植物表现出有用的特征，例如改善的种子大小，降低的末梢核仁流产，在不利的环境条件下增加的种子集合或产量的稳定性。本发明还提供用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包含已知为eep1和eep2的种子优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

6. 发明授权

US07317141B2 Transcriptional activators involved in abiotic stress tolerance 有权
标题翻译：参与非生物胁迫耐受的转录激活因子
公开(公告)号：US07317141B2
公开(公告)日：2008-01-08
申请号：US11386655
申请日：2006-03-22
申请人： Sobhana Sivasankar , Timothy G. Helentjaris , Deping Xu , Anna Lyznik
发明人： Sobhana Sivasankar , Timothy G. Helentjaris , Deping Xu , Anna Lyznik
IPC分类号： C12N15/82
CPC分类号： C12N15/8273 , C07K14/415
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise novel nucleic acid sequences encoding a transcription factor involved in modulating gene expression in response to abiotic stress such as cold or drought. Methods for expressing the nucleic acid sequence in a plant and improving cold and/or drought tolerance of plants are also provided.
摘要翻译：本发明提供调节植物中核苷酸序列表达的组合物和方法。组合物包含编码参与调节基因表达以响应非生物胁迫如冷或干旱的转录因子的新型核酸序列。还提供了在植物中表达核酸序列并改善植物的耐冷和/或耐旱性的方法。

7. 发明授权

US07074985B2 Development of a stress-responsive promoter from maize 失效
公开(公告)号：US07074985B2
公开(公告)日：2006-07-11
申请号：US10365049
申请日：2003-02-12
申请人： Timothy G. Helentjaris , Hank W. Bass , Rebecca S. Boston
发明人： Timothy G. Helentjaris , Hank W. Bass , Rebecca S. Boston
IPC分类号： C12N15/09 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8237 , C07K14/415
摘要： The present invention provides a composition and method for regulating expression of heterologous nucleotide sequences in a plant. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the stress-responsive promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.

8. 发明授权

US06930225B2 Maize cellulose synthases and uses thereof 失效
标题翻译：玉米纤维素合成酶及其用途
公开(公告)号：US06930225B2
公开(公告)日：2005-08-16
申请号：US10209059
申请日：2002-07-31
申请人： Kanwarpal S. Dhugga , Haiyin Wang , Dwight Tomes , Timothy G. Helentjaris
发明人： Kanwarpal S. Dhugga , Haiyin Wang , Dwight Tomes , Timothy G. Helentjaris
IPC分类号： C07K14/415 , C12N9/10 , C12N15/82 , C12N15/09 , C12N15/29 , A01H5/00 , A01H5/10
CPC分类号： C07K14/415 , C12N9/1059 , C12N15/8246 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants comprising said nucleic acids.
摘要翻译：本发明提供分离的纤维素合酶核酸及其编码的蛋白质。本发明提供了关于改变植物中纤维素合成酶水平的方法和组合物。本发明还提供重组表达盒，宿主细胞和包含所述核酸的转基因植物。

9. 发明授权

US06713666B2 Invertase inhibitors and methods of use 失效
标题翻译：转化酶抑制剂和使用方法
公开(公告)号：US06713666B2
公开(公告)日：2004-03-30
申请号：US09780717
申请日：2001-02-09
申请人： Timothy G. Helentjaris , Nicholas J. Bate , Stephen M. Allen
发明人： Timothy G. Helentjaris , Nicholas J. Bate , Stephen M. Allen
IPC分类号： A01H500
CPC分类号： C07K14/415 , C12N15/8245
摘要： Methods and compositions for increasing yield in plants, particularly seed plants, are provided. The compositions comprise novel nucleic acid molecules encoding invertase inhibitors, antisense nucleotides corresponding to invertase inhibitors, and variants and fragments thereof. Such compositions find use in methods to modulate invertase activity in plants. The compositions are also useful in methods to modulate kernel development and for protecting plants against the harmful/detrimental effects of stress and adverse environmental conditions. The nucleotide sequences may be provided in constructs for temporal, developmental, and tissue preference. Transformed plants, plant cells, tissues, and seeds are additionally provided.
摘要翻译：提供了用于提高植物，特别是种子植物产量的方法和组合物。组合物包含编码转化酶抑制剂的新型核酸分子，对应于转化酶抑制剂的反义核苷酸，及其变体和片段。这样的组合物可用于调节植物中的转化酶活性的方法。该组合物还可用于调节内核发育和保护植物免受应激和不利环境条件的有害/不利影响的方法。核苷酸序列可以在时间，发育和组织偏好的构建体中提供。另外提供转化的植物，植物细胞，组织和种子。

10. 发明授权

US07851597B2 Maize cellulose synthases and uses thereof 有权
公开(公告)号：US07851597B2
公开(公告)日：2010-12-14
申请号：US12486129
申请日：2009-06-17
申请人： Kanwarpal S Dhugga , Haiyin Wang , Dwight Tomes , Timothy G Helentjaris
发明人： Kanwarpal S Dhugga , Haiyin Wang , Dwight Tomes , Timothy G Helentjaris
IPC分类号： C07K4/10 , C07K17/12 , C12N15/09
CPC分类号： C12N9/1059 , C07K14/415 , C12N15/8241 , C12N15/8246 , C12N15/8251 , C12N15/8261 , Y02A40/146 , Y02E50/17
摘要： The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants comprising said nucleic acids.

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