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1. 发明授权

US11041856B2 Use of fluorescence for the quick and easy determination of S-adenosylmethionine, S-adenosylhomocysteine and homocysteine 有权
公开(公告)号：US11041856B2
公开(公告)日：2021-06-22
申请号：US16120418
申请日：2018-09-03
申请人： Xiujuan Hao , Chaoyi Deng
发明人： Xiujuan Hao , Chaoyi Deng
IPC分类号： G01N33/543 , G01N33/533 , A61K49/00 , C07K16/44
摘要： The invention provides immunochromatographic test strips and methods and systems or kits for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.

2. 发明授权

US10656159B2 Immunoassays of s-adenosylmethionine and methylation index in personalized medicine and health evaluation 有权
公开(公告)号：US10656159B2
公开(公告)日：2020-05-19
申请号：US15888455
申请日：2018-02-05
申请人： Xiujuan Hao , Isaac A Angres
发明人： Xiujuan Hao , Isaac A Angres
IPC分类号： G01N33/68 , C07K16/44 , A61K31/7076 , C07H19/16 , G01N33/574
摘要： The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

3. 发明申请

US20180169128A1 IMMUNOASSAY OF S-ADENOSYLMETHIONINE USING ANALOGS THEREOF AND PERSONALIZED THERAPEUTICS 审中-公开
公开(公告)号：US20180169128A1
公开(公告)日：2018-06-21
申请号：US15881817
申请日：2018-01-29
申请人： Xiujuan Hao , Isaac Angres
发明人： Xiujuan Hao , Isaac Angres
IPC分类号： A61K31/7076 , G01N33/574
CPC分类号： A61K31/7076 , G01N33/57484 , G01N2800/7033
摘要： A method of detecting the presence or absence of a disease in a patient wherein said disease is accompanied by deficient levels of S-adenosylmethionine comprising: identifying a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of S-adenosylmethionine in said biological sample using an antibody derived from a hapten analog of S-adenosylmethionine; and correlating the level of S-adenosylmethionine in said biological sample with the presence or absence of said disease. The invention also provides methods for measuring SAH which is used to determine the methylation index (ratio of SAM/SAH) in biological fluids which is indicative of the health status of an individual.

4. 发明授权

US10138267B2 Bioconjugates of heterocyclic compounds 有权
公开(公告)号：US10138267B2
公开(公告)日：2018-11-27
申请号：US15091544
申请日：2016-04-05
申请人： Xiujuan Hao
发明人： Xiujuan Hao
IPC分类号： C07H19/167 , C07J43/00 , G01N33/58 , G01N33/68
摘要： The invention provides bioconjugates of heterocylic compounds such as S-adenosylmethionine and S-adenosylhomocysteine with biotin or digoxigenin. The bioconjugates also include carbon and nitrogen linker moieties of varying length that are used to attach such compounds to biotin or digoxigenin. The conjugates are useful in immunoassays. The invention provides a method for detecting SAM and SAH, comprising the steps of: (a) preparing the following components: (i) bio-conjugates of SAM, SAM analogs or SAH; (ii) an europium, a terbium cryptate or other fluorophore as a donor that has a specific ligand for the tracer in the bio-conjugates of (i); (iii) an acceptor fluorescent dye that has the excitation spectra overlap those of donor's emissions and has an antibody specific for SAM or SAH labeled; (b) addition of the biological fluid containing said SAM or SAH; and (c) spectroscopic measurement of the fluorescence of the donor and the fluorescence of from the acceptor.

5. 发明申请

US20180156816A1 IMMUNOASSAYS OF S-ADENOSYLMETHIONINE AND METHYLATION INDEX IN PERSONALIZED MEDICINE AND HEALTH EVALUATION 审中-公开
公开(公告)号：US20180156816A1
公开(公告)日：2018-06-07
申请号：US15888455
申请日：2018-02-05
申请人： Xiujuan Hao , Isaac A Angres
发明人： Xiujuan Hao , Isaac A Angres
IPC分类号： G01N33/68 , C07H19/16 , C07K16/44 , G01N33/574 , A61K31/7076
CPC分类号： G01N33/6812 , A61K31/7076 , C07H19/16 , C07K16/44 , C07K2317/20 , C07K2317/33 , G01N33/57484 , G01N2440/12 , G01N2800/105 , G01N2800/7033 , Y02A50/423
摘要： The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

6. 发明授权

US09885723B2 Immunoassays of S-adenosylmethionine and methylation index in personalized medicine and health evaluation 有权
公开(公告)号：US09885723B2
公开(公告)日：2018-02-06
申请号：US14732775
申请日：2015-06-07
申请人： Xiujuan Hao , Isaac A Angres
发明人： Xiujuan Hao , Isaac A Angres
IPC分类号： A61K31/7076 , G01N33/68 , C07H19/16 , C07K16/44 , G01N33/574
CPC分类号： G01N33/6812 , A61K31/7076 , C07H19/16 , C07K16/44 , C07K2317/20 , C07K2317/33 , G01N33/57484 , G01N2440/12 , G01N2800/105 , G01N2800/7033 , Y02A50/423
摘要： The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

7. 发明申请

US20160291014A1 BIOCONJUGATES OF HETEROCYCLIC COMPOUNDS 审中-公开
标题翻译：杂环化合物的生物活性
公开(公告)号：US20160291014A1
公开(公告)日：2016-10-06
申请号：US15091544
申请日：2016-04-05
申请人： Xiujuan Hao
发明人： Xiujuan Hao
IPC分类号： G01N33/569 , C07J43/00 , C07H19/16
CPC分类号： C07J43/003 , C07H19/167 , G01N33/58 , G01N33/6815
摘要： The invention provides bioconjugates of heterocylic compounds such as S-adenosylmethionine and S-adenosylhomocysteine with biotin or digoxigenin. The bioconjugates also include carbon and nitrogen linker moieties of varying length that are used to attach such compounds to biotin or digoxigenin. The conjugates are useful in immunoassays. The invention provides a method for detecting SAM and SAH, comprising the steps of: (a) preparing the following components: (i) bio-conjugates of SAM, SAM analogs or SAH; (ii) an europium, a terbium cryptate or other fluorophore as a donor that has a specific ligand for the tracer in the bio-conjugates of (i); (iii) an acceptor fluorescent dye that has the excitation spectra overlap those of donor's emissions and has an antibody specific for SAM or SAH labeled; (b) addition of the biological fluid containing said SAM or SAH; and (c) spectroscopic measurement of the fluorescence of the donor and the fluorescence of from the acceptor.
摘要翻译：本发明提供了诸如S-腺苷甲硫氨酸和S-腺苷高半胱氨酸与生物素或地高辛的杂环化合物的生物缀合物。生物缀合物还包括用于将这些化合物连接到生物素或地高辛的不同长度的碳和氮接头部分。缀合物可用于免疫测定。本发明提供了一种检测SAM和SAH的方法，包括以下步骤：（a）制备以下组分：（i）SAM，SAM类似物或SAH的生物缀合物; （ii）铕，铽穴状物或其他荧光团作为供体，其具有（i）生物缀合物中示踪剂的特异性配体; （iii）受体荧光染料，其激发光谱与供体的排放重叠，并具有针对SAM或SAH标记的抗体; （b）加入含有所述SAM或SAH的生物液体; 和（c）供体荧光的光谱测定和受体的荧光。

8. 发明申请

US20180372735A1 USE OF FLUORESCENCE FOR THE QUICK AND EASY DETERMINATION OF S-ADENOSYLMETHIONINE, S-ADENOSYLHOMOCYSTEINE AND HOMOCYSTEINE 审中-公开
公开(公告)号：US20180372735A1
公开(公告)日：2018-12-27
申请号：US16120418
申请日：2018-09-03
申请人： Xiujuan Hao , Chaoyi Deng
发明人： Xiujuan Hao , Chaoyi Deng
IPC分类号： G01N33/543 , G01N33/533
摘要： The invention provides immunochromatographic test strips and methods and systems or kits for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.

9. 发明申请

US20160349251A1 USE OF FLUORESCENCE FOR THE QUICK AND EASY DETERMINATION OF S-ADENOSYLMETHIONINE, S-ADENOSYLHOMOCYSTEINE AND HOMOCYSTEINE 审中-公开
标题翻译：使用荧光快速，简便地测定S-腺苷甲硫氨酸，S-苯二甲酸和高分子量
公开(公告)号：US20160349251A1
公开(公告)日：2016-12-01
申请号：US15164856
申请日：2016-05-25
申请人： Xiujuan Hao , Junhua Wu , Chaoyi Deng
发明人： Xiujuan Hao , Junhua Wu , Chaoyi Deng
IPC分类号： G01N33/543
CPC分类号： G01N33/54373 , A61K49/0058 , C07K16/44 , G01N33/533 , G01N2333/4737 , G01N2800/50
摘要： The invention provides immunochromatographic test strips and methods for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.
摘要翻译：本发明提供免疫色谱测试条和用于检测和定量样品中S-腺苷甲硫氨酸（SAM），S-腺苷高半胱氨酸（SAH）和同型半胱氨酸（HCy）的方法，包括：（a）制备荧光团缀合的抗体; （b）将SAM，SAH和HCY固定在固体支持物上; （c）提供样品，将所述样品与选自镧系元素螯合物缀合物和量子点缀合物（QD）的共轭物与抗SAM，抗SAH或抗-HCi组合，其中所述组合在以下条件下进行：允许在固体支持物上形成包含所述缀合物，所述SAM，SAH或HCy的竞争性复合物，当存在时，样品中的SAM，SAH或HCy; 和（d）通过监测由络合物中的荧光共轭物介导的光谱发射来检测复合物的存在（如果存在），其中发射指示样品中SAM，SAH或HCy的存在和数量。

10. 发明授权

US10067126B2 Use of fluorescence for the quick and easy determination of s-adenosylmethionine, s-adenosylhomocysteine and homocysteine 有权
公开(公告)号：US10067126B2
公开(公告)日：2018-09-04
申请号：US15164856
申请日：2016-05-25
申请人： Xiujuan Hao , Junhua Wu , Chaoyi Deng
发明人： Xiujuan Hao , Junhua Wu , Chaoyi Deng
IPC分类号： G01N33/533 , C07K16/44 , G01N33/543 , A61K49/00
摘要： The invention provides immunochromatographic test strips and methods for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.

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