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1. 发明申请

US20110043213A1 FET-BASED SENSOR FOR DETECTING IONIC MATERIAL, IONIC MATERIAL DETECTING DEVICE USING THE FET-BASED SENSOR, AND METHOD OF DETECTING IONIC MATERIAL USING THE FET-BASED SENSOR 有权
标题翻译：用于检测离子材料的基于FET的传感器，使用基于FET的传感器的离子材料检测装置以及使用基于FET的传感器检测离子材料的方法
公开(公告)号：US20110043213A1
公开(公告)日：2011-02-24
申请号：US12912191
申请日：2010-10-26
申请人： Kyu-sang LEE , Kyu-tae YOO , Jeo-young SHIM , Jin-tae KIM , Yeon-ja CHO
发明人： Kyu-sang LEE , Kyu-tae YOO , Jeo-young SHIM , Jin-tae KIM , Yeon-ja CHO
IPC分类号： G01N27/62
CPC分类号： G01N33/54373 , C12Q1/6825 , G01N27/4145
摘要： Provided are a FET-based sensor for detecting an ionic material, an ionic material detecting device including the FET-based sensor, and a method of detecting an ionic material using the FET-based sensor. The FET-based sensor includes: a sensing chamber including a reference electrode and a plurality of sensing FETs; and a reference chamber including a reference electrode and a plurality of reference FETs. The method includes: flowing a first solution into and out of the sensing chamber and the reference chamber of the FET-based sensor; flowing a second solution expected to contain an ionic material into and out of the sensing chamber while continuously flowing the first solution into and out of the reference chamber; measuring a current in a channel region between the source and drain of each of the sensing and reference FETs; and correcting the current of the sensing FETs.
摘要翻译：提供了一种用于检测离子材料的基于FET的传感器，包括基于FET的传感器的离子材料检测装置，以及使用基于FET的传感器检测离子材料的方法。基于FET的传感器包括：感测室，包括参考电极和多个感测FET; 以及包括参考电极和多个参考FET的参考室。该方法包括：使第一溶液流入和流出基于FET的传感器的感测室和参考室; 使预期含有离子材料的第二溶液流入和流出感测室，同时使第一溶液持续流入和移出参考室; 测量每个感测和参考FET的源极和漏极之间的沟道区域中的电流; 并校正感测FET的电流。

2. 发明授权

US07785787B2 Methods of isolating and amplifying nucleic acids using silanized solid support 有权
标题翻译：使用硅烷化固体支持物分离和扩增核酸的方法
公开(公告)号：US07785787B2
公开(公告)日：2010-08-31
申请号：US11345174
申请日：2006-02-01
申请人： Myo-yong Lee , Joong-gun Lee , Young-nam Kwon , Young-a Kim , Yeon-ja Cho , Shin-i Yoo
发明人： Myo-yong Lee , Joong-gun Lee , Young-nam Kwon , Young-a Kim , Yeon-ja Cho , Shin-i Yoo
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C07H21/04 , C12N15/1006
摘要： Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.
摘要翻译：提供从含核酸样品中分离和扩增核酸的方法。核酸分离方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化的固体支持物，并用pH9至14的碱性溶液处理核酸捕获的固体支持物。核酸扩增方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化固体支持物; 用pH9至14的碱性溶液处理核酸捕获的固体支持物; 并在碱溶液处理后向所得溶液中加入核酸扩增溶液进行核酸扩增。

3. 发明申请

US20060084165A1 Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
标题翻译：使用微磁珠和激光快速破坏细胞或病毒的方法和装置
公开(公告)号：US20060084165A1
公开(公告)日：2006-04-20
申请号：US11253541
申请日：2005-10-19
申请人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
发明人： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
IPC分类号： C12N1/06
CPC分类号： C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
摘要翻译：提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决裂解芯片可应用于LOC。

4. 发明授权

US08038943B2 Method of detecting bio-molecules using field effect transistor without fixing probe bio-molecules on the gate sensing surface 有权
标题翻译：使用场效应晶体管检测生物分子的方法，而不将探针生物分子固定在栅极感测表面上
公开(公告)号：US08038943B2
公开(公告)日：2011-10-18
申请号：US11695905
申请日：2007-04-03
申请人： Kyu-tae Yoo , Kyu-sang Lee , Won-seok Chung , Jeo-young Shim , Yeon-ja Cho
发明人： Kyu-tae Yoo , Kyu-sang Lee , Won-seok Chung , Jeo-young Shim , Yeon-ja Cho
IPC分类号： G01N27/00
CPC分类号： G01N33/54373
摘要： A method of detecting a presence of bio-molecules, or a concentration of the target bio-molecules using a field effect transistor, includes allowing a first sample including a first target bio-molecule to contact a sensing surface of the field effect transistor and measuring a change in an electric signal of the field effect transistor, the field effect transistor including a substrate, a source region and a drain region, the source region and the drain region formed apart from each other on the substrate, the source region and the drain region each doped to having an opposite polarity than a polarity of the substrate, a channel region disposed between the source region and the drain region and an insulating layer including the sensing surface, the insulating layer disposed on the channel region.
摘要翻译：使用场效应晶体管检测生物分子的存在或靶生物分子的浓度的方法包括允许包含第一靶生物分子的第一样品接触场效应晶体管的感测表面并测量场效应晶体管的电信号的变化，所述场效应晶体管包括基板，源极区和漏极区，在所述基板上彼此分离形成的所述源极区和漏极区，所述源极区和漏极每个被掺杂为具有与衬底的极性相反的极性，设置在源区和漏区之间的沟道区和包括感测表面的绝缘层，绝缘层设置在沟道区上。

5. 发明授权

US07859029B2 FET-based sensor for detecting ionic material, ionic material detecting device using the FET-based sensor, and method of detecting ionic material using the FET-based sensor 有权
标题翻译：用于检测离子材料的基于FET的传感器，使用基于FET的传感器的离子材料检测装置以及使用基于FET的传感器检测离子材料的方法
公开(公告)号：US07859029B2
公开(公告)日：2010-12-28
申请号：US11621191
申请日：2007-01-09
申请人： Kyu-sang Lee , Kyu-tae Yoo , Jeo-young Shim , Jin-tae Kim , Yeon-ja Cho
发明人： Kyu-sang Lee , Kyu-tae Yoo , Jeo-young Shim , Jin-tae Kim , Yeon-ja Cho
IPC分类号： H01L29/78
CPC分类号： G01N33/54373 , C12Q1/6825 , G01N27/4145
摘要： Provided are a FET-based sensor for detecting an ionic material, an ionic material detecting device including the FET-based sensor, and a method of detecting an ionic material using the FET-based sensor. The FET-based sensor includes: a sensing chamber including a reference electrode and a plurality of sensing FETs; and a reference chamber including a reference electrode and a plurality of reference FETs. The method includes: flowing a first solution into and out of the sensing chamber and the reference chamber of the FET-based sensor; flowing a second solution expected to contain an ionic material into and out of the sensing chamber while continuously flowing the first solution into and out of the reference chamber; measuring a current in a channel region between the source and drain of each of the sensing and reference FETs; and correcting the current of the sensing FETs.
摘要翻译：提供了一种用于检测离子材料的基于FET的传感器，包括基于FET的传感器的离子材料检测装置，以及使用基于FET的传感器检测离子材料的方法。基于FET的传感器包括：感测室，包括参考电极和多个感测FET; 以及包括参考电极和多个参考FET的参考室。该方法包括：使第一溶液流入和流出基于FET的传感器的感测室和参考室; 使预期含有离子材料的第二溶液流入和流出感测室，同时使第一溶液持续流入和移出参考室; 测量每个感测和参考FET的源极和漏极之间的沟道区域中的电流; 并校正感测FET的电流。

6. 发明授权

US07839134B2 Method and apparatus for simultaneously detecting size and concentration of ionic material 有权
标题翻译：用于同时检测离子材料的尺寸和浓度的方法和装置
公开(公告)号：US07839134B2
公开(公告)日：2010-11-23
申请号：US11619650
申请日：2007-01-04
申请人： Kyu-sang Lee , Kyu-tae Yoo , Jeo-young Shim , Won-seok Chung , Yeon-ja Cho
发明人： Kyu-sang Lee , Kyu-tae Yoo , Jeo-young Shim , Won-seok Chung , Yeon-ja Cho
IPC分类号： G01N27/00
CPC分类号： G01N27/4145 , G01N27/27
摘要： A method for simultaneously detecting a size and concentration of ionic materials includes measuring voltage drop values of at least three ionic materials of which sizes and concentrations are known using each of at least two FET-based sensors having different electrical characteristics, determining at least three points in a three-dimensional plot from the known sizes, concentrations and the measured voltage drop values, approximating the at least three points into a single plane, measuring a voltage drop value of an ionic material of which size and concentration are unknown using the at least two FET-based sensors, determining equipotential lines existing on the plane using the voltage drop value of the unknown ionic material and determining a cross point between each of the equipotential lines.
摘要翻译：用于同时检测离子材料的尺寸和浓度的方法包括测量至少三种离子材料的电压降值，其中尺寸和浓度已知使用具有不同电特性的至少两个基于FET的传感器中的每一个，确定至少三个点在已知尺寸，浓度和测量的电压降值的三维图中，将至少三个点近似为单个平面，测量其尺寸和浓度未知的离子材料的电压降值，至少使用至少两个基于FET的传感器，使用未知离子材料的电压降值确定在平面上存在的等电位线，并确定每个等电位线之间的交叉点。

7. 发明申请

US20090153130A1 FIELD EFFECT TRANSISTOR-BASED BIOSENSOR WITH INORGANIC FILM, METHOD OF MANUFACTURING THE BIOSENSOR, AND METHOD OF DETECTING BIOMOLECULE USING THE BIOSENSOR 审中-公开
标题翻译：基于场效应晶体管的无机膜生物传感器，生物传感器的制造方法和使用生物传感器检测生物分子的方法
公开(公告)号：US20090153130A1
公开(公告)日：2009-06-18
申请号：US11556783
申请日：2006-11-06
申请人： Jeo-young SHIM , Kyu-sang LEE , Chang-eun YOO , Kyu-youn HWANG , Young-a KIM , Kyu-tae YOO , Yeon-ja CHO
发明人： Jeo-young SHIM , Kyu-sang LEE , Chang-eun YOO , Kyu-youn HWANG , Young-a KIM , Kyu-tae YOO , Yeon-ja CHO
IPC分类号： G01N27/00 , H01L29/772 , H01L21/335
CPC分类号： G01N33/54373 , B01J2219/00529 , B01J2219/00596 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00722 , G01N27/4145
摘要： Provided is a Field-Effect Transistor (FET)-based biosensor including: a substrate; a source and a drain, disposed on the substrate, having opposite polarity to the substrate; a gate, disposed on the substrate, contacting the source and the drain; and an inorganic film capable of binding with a biomolecule, disposed on a surface of the gate. A method of manufacturing the FET-based biosensor and a method of detecting a biomolecule using the FET-based biosensor is also provided. The FET-based biosensor can be manufactured using a semiconductor fabrication process without performing an additional process. Therefore, the inorganic film can be selectively deposited on a surface of a specific gate of a single FET, or on the surfaces of some gates of a plurality of FETs using patterning. Furthermore, the FET-based biosensor can be used to effectively detect trace amounts of a target biomolecule in a sample.
摘要翻译：提供了一种基于场效应晶体管（FET）的生物传感器，包括：基板; 源极和漏极，设置在衬底上，具有与衬底相反的极性; 栅极，设置在基板上，与源极和漏极接触; 以及能够与生物分子结合的无机膜，其设置在栅极的表面上。还提供了制造基于FET的生物传感器的方法和使用基于FET的生物传感器检测生物分子的方法。基于FET的生物传感器可以使用半导体制造工艺制造而不执行额外的工艺。因此，无机膜可以选择性地沉积在单个FET的特定栅极的表面上，或者使用图案化沉积在多个FET的一些栅极的表面上。此外，基于FET的生物传感器可以用于有效地检测样品中痕量的目标生物分子。

8. 发明申请

US20060110725A1 Apparatus for and method of purifying nucleic acids by different laser absorption of beads 审中-公开
标题翻译：通过不同激光吸收珠粒来纯化核酸的装置和方法
公开(公告)号：US20060110725A1
公开(公告)日：2006-05-25
申请号：US11190169
申请日：2005-07-26
申请人： Jeong-gun Lee , Young-nam Kwon , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Young-a Kim
发明人： Jeong-gun Lee , Young-nam Kwon , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Young-a Kim
IPC分类号： C12Q1/70 , C12Q1/68 , C12N7/02 , C12N1/08 , C12M1/34
CPC分类号： B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0647 , B01L2200/10 , B01L2300/0681 , B01L2300/0867 , B01L2300/087 , B01L2400/0487
摘要： An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples, magnetic beads, and solid support in the capillary; a laser generator attached to the capillary and irradiating a laser beam onto the capillary; a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall; a waste chamber attached to the capillary and discharging a lysate; an elution buffer chamber attached to the capillary and eluting nucleic acids from the solid support having nucleic acids bound thereto; and a neutralization buffer chamber attached to the capillary and supplying a neutralization buffer for neutralizing the eluted nucleic acid solution. According to the apparatus and method, PCR inhibitors can be removed to increase PCR yield and nucleic acids can be purified using a silicon substrate or silica beads. Thus, the apparatus and method can be applied to LOC fabrication.
摘要翻译：提供了一种纯化细胞或病毒核酸的装置和方法。核酸纯化装置包括：细胞裂解毛细管，其具有样品入口，通过所述样品入口引入样品，磁珠和固体支持物; 连接到毛细管并将样品，磁珠和固体支持物混合在毛细管中的振动器; 激光发生器附接到毛细管并将激光束照射到毛细管上; 附着到毛细管并将磁珠固定到毛细管壁的磁力发生器; 连接到毛细管并排出裂解物的废物室; 洗脱缓冲室连接到毛细管并从具有与其结合的核酸的固体支持物洗脱核酸; 以及中和缓冲室，其连接到毛细管并提供用于中和洗脱的核酸溶液的中和缓冲液。根据该装置和方法，可以除去PCR抑制剂以增加PCR产量，并且可以使用硅底物或二氧化硅珠纯化核酸。因此，该装置和方法可以应用于LOC制造。

9. 发明授权

US07943970B2 Method of detecting bio-molecules using the same field effect transistor on the gate sensing surface 有权
标题翻译：使用栅极感应表面上相同的场效应晶体管检测生物分子的方法
公开(公告)号：US07943970B2
公开(公告)日：2011-05-17
申请号：US11695886
申请日：2007-04-03
申请人： Kyu-tae Yoo , Kyu-sang Lee , Jeo-young Shim , Won-seok Chung , Yeon-ja Cho
发明人： Kyu-tae Yoo , Kyu-sang Lee , Jeo-young Shim , Won-seok Chung , Yeon-ja Cho
IPC分类号： G01N27/403
CPC分类号： G01N33/54373 , G01N27/4145
摘要： Provided is a method of detecting the presence of a target bio-molecule or a concentration of the bio-molecule using a field effect transistor. The method includes: contacting a first sample having a first target bio-molecule with a reference electrode of a field effect transistor; measuring a first electric signal change of the field effect transistor; contacting a second sample with a sensing surface of the same field effect transistor; measuring a second electric signal change of the field effect transistor; and comparing the first electric signal with the second electric signal.
摘要翻译：提供了使用场效应晶体管检测目标生物分子的存在或生物分子的浓度的方法。该方法包括：将具有第一目标生物分子的第一样品与场效应晶体管的参比电极接触; 测量场效应晶体管的第一电信号变化; 使第二样品与相同场效应晶体管的感测表面接触; 测量场效应晶体管的第二电信号变化; 以及将所述第一电信号与所述第二电信号进行比较。

10. 发明申请

US20080199930A1 METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER 审中-公开
标题翻译：使用微珠和激光快速破坏细胞或病毒的方法和装置
公开(公告)号：US20080199930A1
公开(公告)日：2008-08-21
申请号：US12031947
申请日：2008-02-15
申请人： Jeong-gun LEE , Young-nam KWON , Young-a KIM , Myo-yong LEE , Shin-i YOO , Yeon-ja CHO , Kwang-ho CHEONG , Chang-eun YOO , Seung-yeon YANG
发明人： Jeong-gun LEE , Young-nam KWON , Young-a KIM , Myo-yong LEE , Shin-i YOO , Yeon-ja CHO , Kwang-ho CHEONG , Chang-eun YOO , Seung-yeon YANG
IPC分类号： C12N13/00 , C12M1/00
CPC分类号： C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
摘要： A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
摘要翻译：提供了使用珠和激光来快速破坏细胞或病毒的方法和装置。根据使用珠粒和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，可以使用激光二极管使装置小型化，DNA纯化步骤可以在细胞破碎后直接进行或病毒，并且含有DNA的溶液可以转移到随后的步骤中，在用电磁体除去后续反应的抑制剂附着的细胞碎片和珠粒之后。另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决裂解芯片可应用于LOC。

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