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    • 1. 发明授权
    • Thermally-tolerant pectin methylesterase
    • 耐热果胶甲酯酶
    • US07803597B2
    • 2010-09-28
    • US11986187
    • 2007-11-20
    • Brett J. SavaryRandall G. CameronGary A. LuzioThomas G. McCollumPrasanna VasuAlberto Nunez
    • Brett J. SavaryRandall G. CameronGary A. LuzioThomas G. McCollumPrasanna VasuAlberto Nunez
    • C12N9/00C12N9/16C12N9/18C12N1/20C12N15/00C07H21/04
    • C12N9/18C12Y301/01011
    • Enzymes accumulated in plant cell walls serve diverse physiological functions including metabolism, polysaccharide structure modification, and molecular communication in interactions with other organisms. Pectin methylesterases are economically important enzymes for their impact on quality and processing properties of fruit and vegetable food products. We have now purified TT-PME to homogeneity from sweet orange finisher pulp and determined the complete corresponding nucleic acid sequence. Purified TT-PME was observed by SDS-PAGE as two doublet bands with molecular masses of approximately 46,000 Da and 56,000 Da. Direct Edman sequencing from these proteins showed a common N-terminal peptide. De novo sequencing of eight TT-PME tryptic peptides determined by MALDI-TOF/TOF mass spectrometry provided additional internal sequences. TT-PME did not correspond to any previously reported Citrus spp. PME sequence. Our results show Citrus TT-PME is a distinctive new isoform with phylogenetic relationship closer to PME isoforms in other species rather than to previously described Citrus PME genes.
    • 在植物细胞壁中积累的酶可以产生不同的生理功能,包括代谢,多糖结构修饰以及与其他生物的相互作用的分子交流。 果胶甲酯酶是影响水果和蔬菜食品质量和加工性能的重要酶。 我们现在已经从甜橙整理纸浆中将TT-PME纯化为均匀,并确定完整的相应的核酸序列。 通过SDS-PAGE观察纯化的TT-PME作为分子量为约46,000Da和56,000Da的两个双峰带。 来自这些蛋白质的直接Edman测序显示出常见的N-末端肽。 通过MALDI-TOF / TOF质谱法测定的8种TT-PME胰蛋白酶肽的重新测序提供了额外的内部序列。 TT-PME不符合任何以前报道的柑橘属物种。 PME序列。 我们的研究结果显示柑橘TT-PME是一种独特的新型异构体,其系统发育关系更接近其他物种的PME同种型,而不是以前描述的柑橘PME基因。
    • 2. 发明申请
    • Thermally-tolerant pectin methylesterase
    • 耐热果胶甲酯酶
    • US20090130722A1
    • 2009-05-21
    • US11986187
    • 2007-11-20
    • Brett J. SavaryRandall G. CameronGary A. LuzioThomas G. McCollumPrasanna VasuAlberto Nunez
    • Brett J. SavaryRandall G. CameronGary A. LuzioThomas G. McCollumPrasanna VasuAlberto Nunez
    • C12P19/04C07H21/04C12N9/10
    • C12N9/18C12Y301/01011
    • Enzymes accumulated in plant cell walls serve diverse physiological functions including metabolism, polysaccharide structure modification, and molecular communication in interactions with other organisms. Pectin methylesterases are economically important enzymes for their impact on quality and processing properties of fruit and vegetable food products. We have now purified TT-PME to homogeneity from sweet orange finisher pulp and determined the complete corresponding nucleic acid sequence. Purified TT-PME was observed by SDS-PAGE as two doublet bands with molecular masses of approximately 46,000 Da and 56,000 Da. Direct Edman sequencing from these proteins showed a common N-terminal peptide. De novo sequencing of eight TT-PME tryptic peptides determined by MALDI-TOF/TOF mass spectrometry provided additional internal sequences. TT-PME did not correspond to any previously reported Citrus spp. PME sequence. Our results show Citrus TT-PME is a distinctive new isoform with phylogenetic relationship closer to PME isoforms in other species rather than to previously described Citrus PME genes.
    • 在植物细胞壁中积累的酶可以产生不同的生理功能,包括代谢,多糖结构修饰以及与其他生物的相互作用的分子交流。 果胶甲酯酶是影响水果和蔬菜食品质量和加工性能的重要酶。 我们现在已经从甜橙整理纸浆中将TT-PME纯化为均匀,并确定完整的相应的核酸序列。 通过SDS-PAGE观察纯化的TT-PME作为分子量为约46,000Da和56,000Da的两个双峰带。 来自这些蛋白质的直接Edman测序显示出常见的N-末端肽。 通过MALDI-TOF / TOF质谱法测定的8种TT-PME胰蛋白酶肽的重新测序提供了额外的内部序列。 TT-PME不符合任何以前报道的柑橘属物种。 PME序列。 我们的研究结果显示柑橘TT-PME是一种独特的新型异构体,其系统发育关系更接近其他物种的PME同种型,而不是以前描述的柑橘PME基因。
    • 4. 发明授权
    • Epoxidation of carbon-carbon double bond with membrane bound peroxygenase
    • 碳膜双键环氧化与膜结合过氧化酶
    • US06852315B2
    • 2005-02-08
    • US10262937
    • 2002-10-03
    • George J. PiazzaThomas A. FogliaAlberto Nunez
    • George J. PiazzaThomas A. FogliaAlberto Nunez
    • C12N9/02C12N11/08C12P17/02A61K38/43A61K35/78
    • C12N9/0071C12N11/08C12P17/02
    • A method has been discovered for the epoxidation of a compound having at least one carbon-carbon double bond, the method involves reacting a compound having at least one carbon-carbon double bond, a solvent, an oxidant, and membrane bound peroxygenase. Also discovered is a method for preparing the membrane bound peroxygenase involving grinding seeds containing peroxygenase to produce ground seeds, homogenizing the ground seeds in a buffer to form a slurry, centrifuging the slurry to produce a first supernatant, centrifuging the first supernatant to produce a second supernatant, and filtering said second supernatant through a protein-binding membrane filter to produce membrane bound peroxygenase; optionally the second supernatant is filtered through a hydrophilic membrane filter prior to filtering the second supernatant through a protein-binding membrane filter.
    • 已经发现了具有至少一个碳 - 碳双键的化合物的环氧化方法,该方法包括使具有至少一个碳 - 碳双键的化合物,溶剂,氧化剂和膜结合的过氧化酶反应。 还发现了一种制备膜结合过氧化酶的方法,包括研磨含有过氧化酶的种子以产生研磨种子,将缓冲液中的研磨种子匀浆以形成浆液,离心该浆料以产生第一上清液,离心第一上清液以产生第二次 上清液,并通过蛋白质结合膜过滤器过滤所述第二上清液以产生膜结合的过氧化酶; 任选地,通过亲和膜过滤器过滤第二上清液,然后通过蛋白质结合膜过滤器过滤第二上清液。
    • 8. 发明授权
    • Epoxidation of carbon-carbon double bond with membrane bound peroxygenase
    • 碳膜双键环氧化与膜结合过氧化酶
    • US06485949B1
    • 2002-11-26
    • US09772301
    • 2001-01-29
    • George J. PiazzaAlberto NunezThomas A. Foglia
    • George J. PiazzaAlberto NunezThomas A. Foglia
    • C12N1108
    • C12N9/0071C12N11/08C12P17/02
    • A method has been discovered for the epoxidation of a compound having at least one carbon-carbon double bond, the method involves reacting a compound having at least one carbon-carbon double bond, a solvent, an oxidant, and membrane bound peroxygenase. Also discovered is a method for preparing the membrane bound peroxygenase involving grinding seeds containing peroxygenase to produce ground seeds, homogenizing the ground seeds in a buffer to form a slurry, centrifuging the slurry to produce a first supernatant, centrifuging the first supernatant to produce a second supernatant, and filtering said second supernatant through a protein-binding membrane filter to produce membrane bound peroxygenase; optionally the second supernatant is filtered through a hydrophilic membrane filter prior to filtering the second supernatant through a protein-binding membrane filter.
    • 已经发现了具有至少一个碳 - 碳双键的化合物的环氧化方法,该方法包括使具有至少一个碳 - 碳双键的化合物,溶剂,氧化剂和膜结合的过氧化酶反应。 还发现了一种制备膜结合过氧化酶的方法,包括研磨含有过氧化酶的种子以产生研磨种子,将缓冲液中的研磨种子匀浆以形成浆液,离心该浆料以产生第一上清液,离心第一上清液以产生第二次 上清液,并通过蛋白质结合膜过滤器过滤所述第二上清液以产生膜结合的过氧化酶; 任选地,通过亲和膜过滤器过滤第二上清液,然后通过蛋白质结合膜过滤器过滤第二上清液。