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1. 发明授权

US07803597B2 Thermally-tolerant pectin methylesterase 有权
标题翻译：耐热果胶甲酯酶
公开(公告)号：US07803597B2
公开(公告)日：2010-09-28
申请号：US11986187
申请日：2007-11-20
申请人： Brett J. Savary , Randall G. Cameron , Gary A. Luzio , Thomas G. McCollum , Prasanna Vasu , Alberto Nunez
发明人： Brett J. Savary , Randall G. Cameron , Gary A. Luzio , Thomas G. McCollum , Prasanna Vasu , Alberto Nunez
IPC分类号： C12N9/00 , C12N9/16 , C12N9/18 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/18 , C12Y301/01011
摘要： Enzymes accumulated in plant cell walls serve diverse physiological functions including metabolism, polysaccharide structure modification, and molecular communication in interactions with other organisms. Pectin methylesterases are economically important enzymes for their impact on quality and processing properties of fruit and vegetable food products. We have now purified TT-PME to homogeneity from sweet orange finisher pulp and determined the complete corresponding nucleic acid sequence. Purified TT-PME was observed by SDS-PAGE as two doublet bands with molecular masses of approximately 46,000 Da and 56,000 Da. Direct Edman sequencing from these proteins showed a common N-terminal peptide. De novo sequencing of eight TT-PME tryptic peptides determined by MALDI-TOF/TOF mass spectrometry provided additional internal sequences. TT-PME did not correspond to any previously reported Citrus spp. PME sequence. Our results show Citrus TT-PME is a distinctive new isoform with phylogenetic relationship closer to PME isoforms in other species rather than to previously described Citrus PME genes.
摘要翻译：在植物细胞壁中积累的酶可以产生不同的生理功能，包括代谢，多糖结构修饰以及与其他生物的相互作用的分子交流。果胶甲酯酶是影响水果和蔬菜食品质量和加工性能的重要酶。我们现在已经从甜橙整理纸浆中将TT-PME纯化为均匀，并确定完整的相应的核酸序列。通过SDS-PAGE观察纯化的TT-PME作为分子量为约46,000Da和56,000Da的两个双峰带。来自这些蛋白质的直接Edman测序显示出常见的N-末端肽。通过MALDI-TOF / TOF质谱法测定的8种TT-PME胰蛋白酶肽的重新测序提供了额外的内部序列。 TT-PME不符合任何以前报道的柑橘属物种。 PME序列。我们的研究结果显示柑橘TT-PME是一种独特的新型异构体，其系统发育关系更接近其他物种的PME同种型，而不是以前描述的柑橘PME基因。

2. 发明申请

US20090130722A1 Thermally-tolerant pectin methylesterase 有权
标题翻译：耐热果胶甲酯酶
公开(公告)号：US20090130722A1
公开(公告)日：2009-05-21
申请号：US11986187
申请日：2007-11-20
申请人： Brett J. Savary , Randall G. Cameron , Gary A. Luzio , Thomas G. McCollum , Prasanna Vasu , Alberto Nunez
发明人： Brett J. Savary , Randall G. Cameron , Gary A. Luzio , Thomas G. McCollum , Prasanna Vasu , Alberto Nunez
IPC分类号： C12P19/04 , C07H21/04 , C12N9/10
CPC分类号： C12N9/18 , C12Y301/01011
摘要： Enzymes accumulated in plant cell walls serve diverse physiological functions including metabolism, polysaccharide structure modification, and molecular communication in interactions with other organisms. Pectin methylesterases are economically important enzymes for their impact on quality and processing properties of fruit and vegetable food products. We have now purified TT-PME to homogeneity from sweet orange finisher pulp and determined the complete corresponding nucleic acid sequence. Purified TT-PME was observed by SDS-PAGE as two doublet bands with molecular masses of approximately 46,000 Da and 56,000 Da. Direct Edman sequencing from these proteins showed a common N-terminal peptide. De novo sequencing of eight TT-PME tryptic peptides determined by MALDI-TOF/TOF mass spectrometry provided additional internal sequences. TT-PME did not correspond to any previously reported Citrus spp. PME sequence. Our results show Citrus TT-PME is a distinctive new isoform with phylogenetic relationship closer to PME isoforms in other species rather than to previously described Citrus PME genes.
摘要翻译：在植物细胞壁中积累的酶可以产生不同的生理功能，包括代谢，多糖结构修饰以及与其他生物的相互作用的分子交流。果胶甲酯酶是影响水果和蔬菜食品质量和加工性能的重要酶。我们现在已经从甜橙整理纸浆中将TT-PME纯化为均匀，并确定完整的相应的核酸序列。通过SDS-PAGE观察纯化的TT-PME作为分子量为约46,000Da和56,000Da的两个双峰带。来自这些蛋白质的直接Edman测序显示出常见的N-末端肽。通过MALDI-TOF / TOF质谱法测定的8种TT-PME胰蛋白酶肽的重新测序提供了额外的内部序列。 TT-PME不符合任何以前报道的柑橘属物种。 PME序列。我们的研究结果显示柑橘TT-PME是一种独特的新型异构体，其系统发育关系更接近其他物种的PME同种型，而不是以前描述的柑橘PME基因。

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