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1. 发明授权

US09753266B2 Method for detecting and controlling supply of an immersion medium 有权
公开(公告)号：US09753266B2
公开(公告)日：2017-09-05
申请号：US14319246
申请日：2014-06-30
申请人： Carl Zeiss Microscopy GmbH
发明人： Ralf Steinmeyer , Reiner Mitzkus , Timo Rojahn
IPC分类号： G02B7/28 , G02B21/24 , G02B21/33
CPC分类号： G02B21/241 , G02B21/244 , G02B21/245 , G02B21/33
摘要： A method for detecting and controlling supply of an immersion medium into an immersion film region between the front lens of a microscope objective and a preparation, particularly in automated microscopes, using a light source which is reflected into the microscope beam path by means of a beam splitter. The state of the immersion film region is recorded and evaluated in a detection unit of the microscope or a detection unit of the autofocus device through an autofocus signal of the light source and/or a reflection of a modulation object arranged in the intermediate image plane of an incident light illumination, and is transmitted to a control unit for the purpose of a change or an error treatment of the immersion process.

2. 发明授权

US09563049B2 Procedure and device for terminating the immersion at a microscope 有权
标题翻译：在显微镜下终止浸渍的方法和装置
公开(公告)号：US09563049B2
公开(公告)日：2017-02-07
申请号：US14617108
申请日：2015-02-09
申请人： Carl Zeiss Microscopy GmbH
发明人： Peter Schön , Timo Rojahn , Alexander Scheps , Reiner Mitzkus
IPC分类号： G02B21/33 , G02B21/24 , G02B21/26
CPC分类号： G02B21/33 , G02B21/248 , G02B21/26
摘要： A procedure and a device for terminating the immersion at a microscope having one or more immersion lenses, or both immersion lenses and dry lenses, comprising a protective sheath around the immersion film area for preventing the escape of immersion media and an immersion media supply unit and an immersion media discharge unit connected with the protective sheath. The immersion media is removed in a controlled manner in particular in an automated microscope. Subsequently, the microscopic testing can be continued without the user having to intervene in the device or the process.
摘要翻译：一种在具有一个或多个浸没透镜或浸没透镜和干透镜的显微镜下终止浸没的程序和装置，包括围绕浸没膜区域的防护护套，用于防止浸没介质和浸没介质供应单元的逸出，以及与保护套连接的浸没介质排出单元。以受控的方式，特别是在自动显微镜中去除浸渍介质。随后，可以继续进行微观测试，而不需要用户干预设备或过程。

3. 发明授权

US10116855B2 Autofocus method for microscope and microscope comprising autofocus device 有权
公开(公告)号：US10116855B2
公开(公告)日：2018-10-30
申请号：US15380947
申请日：2016-12-15
申请人： Carl Zeiss Microscopy GmbH
发明人： Peter Westphal , Reiner Mitzkus
IPC分类号： H04N5/232 , G02B21/24 , G02B7/28 , G02B21/02 , G02B21/36 , G02B21/08
摘要： A microscope including an objective having a focal plane in a sample space, and an autofocus device comprising a light modulator for generating a luminous modulation object that is intensity-modulated periodically along one direction, an autofocus illumination optical unit that images the modulation object such that its image arises in the sample space, an autofocus camera, an autofocus imaging optical unit that images the image of the modulation object in the sample space onto the autofocus camera, a control device, which receives signals of the autofocus camera and determines an intensity distribution of the image of the modulation object and generates a focus control signal therefrom. The control device determines an intensity distribution of the image of a luminous comparison object imaged by the optical unit to correct the intensity distribution of the image of the modulation object with regard to reflectivity variations in the sample space.

4. 发明授权

US09741122B2 Method and device for determining a distance between two optical boundary surfaces which are spaced apart from each other along a first direction 有权
公开(公告)号：US09741122B2
公开(公告)日：2017-08-22
申请号：US14826355
申请日：2015-08-14
申请人： Carl Zeiss Microscopy GmbH
发明人： Reiner Mitzkus , Matthias Pospiech , Anke Vogelgsang
IPC分类号： G06T7/00 , G02B21/24 , G06K9/46 , G06T5/00 , G02B21/00 , G01B9/04 , G01B11/06 , G02B21/36
CPC分类号： G06T7/73 , G01B9/04 , G01B11/06 , G02B21/0016 , G02B21/244 , G02B21/245 , G02B21/367 , G06K9/4604 , G06T5/003 , G06T2207/10028 , G06T2207/10056 , G06T2207/10148
摘要： A method is provided for determining the distance between two optical boundary surfaces spaced apart from each other in a first direction. A first image is ascertained wherein the plane into which the pattern acquired coincides with a first of two optical boundary surfaces or has the smallest distance to the first optical boundary surface in a first direction. A position of the first image in the first direction is determined. A second image is ascertained wherein the plane into which the pattern acquired coincides with a second of two optical boundary surfaces or has the smallest distance to the second optical boundary surface in the first direction. The position of the second image in the first direction is determined. The distance is calculated by means of determined positions of the first and second image.

5. 发明授权

US09389405B2 Autofocus method for microscope and microscope with autofocus device 有权
标题翻译：具有自动对焦装置的显微镜和显微镜的自动对焦方式
公开(公告)号：US09389405B2
公开(公告)日：2016-07-12
申请号：US14106450
申请日：2013-12-13
申请人： Carl Zeiss Microscopy GmbH
发明人： Peter Westphal , Reiner Mitzkus
IPC分类号： G02B21/00 , G02B21/24
CPC分类号： G02B21/245
摘要： An autofocus method for a microscope with an objective which images a sample lying in an object plane, including the steps: projecting a longitudinally extended grating slit which lies in a grating slit plane onto the sample, and imaging the projection of the grating slit onto an autofocus camera; determining an intensity distribution of the grating slit image and from this, deducing a preset for a relative adjustment of sample and object plane; projecting a likewise longitudinally extended comparison slit onto the sample, and imaging the projection of the comparison slit onto the autofocus camera; evaluating the width of the comparison slit image at right angles to the longitudinal extension at at least two sites which are spaced apart along the longitudinal extension, and determining a width variation of the comparison slit image, a gradient of the width variation and a direction of the relative adjustment.
摘要翻译：一种用于显微镜的自动对焦方法，其具有对位于物体平面中的样品进行成像的目标，包括以下步骤：将位于光栅狭缝平面中的纵向延伸的光栅狭缝投影到样品上，以及将光栅狭缝的投影成像到自动对焦相机; 确定光栅狭缝图像的强度分布，并由此推出用于样本和物体平面相对调节的预设; 将同样纵向延伸的比较狭缝投影到样本上，并将比较狭缝的投影成像到自动对焦照相机上; 在沿着纵向延伸部间隔开的至少两个位置处评估与纵向延伸成直角的比较狭缝图像的宽度，并且确定比较狭缝图像的宽度变化，宽度变化的梯度和方向相对调整。

6. 发明申请

US20150260975A1 PROCEDURE AND DEVICE FOR TERMINATING THE IMMERSION AT A MICROSCOPE 有权
标题翻译：用于终止MICROSCOPE注入的程序和设备
公开(公告)号：US20150260975A1
公开(公告)日：2015-09-17
申请号：US14617108
申请日：2015-02-09
申请人： Carl Zeiss Microscopy GmbH
发明人： Peter Schön , Timo Rojahn , Alexander Scheps , Reiner Mitzkus
IPC分类号： G02B21/33 , G02B21/24 , G02B21/26
CPC分类号： G02B21/33 , G02B21/248 , G02B21/26
摘要： A procedure and a device for terminating the immersion at a microscope having one or more immersion lenses, or both immersion lenses and dry lenses, comprising a protective sheath around the immersion film area for preventing the escape of immersion media and an immersion media supply unit and an immersion media discharge unit connected with the protective sheath. The immersion media is removed in a controlled manner in particular in an automated microscope. Subsequently, the microscopic testing can be continued without the user having to intervene in the device or the process.
摘要翻译：一种在具有一个或多个浸没透镜或浸没透镜和干透镜的显微镜下终止浸没的程序和装置，包括围绕浸没膜区域的防护护套，用于防止浸没介质和浸没介质供应单元的逸出，以及与保护套连接的浸没介质排出单元。以受控的方式，特别是在自动显微镜中去除浸渍介质。随后，可以继续进行微观测试，而不需要用户干预设备或过程。

7. 发明申请

US20150015942A1 METHOD FOR DETECTING AND CONTROLLING SUPPLY OF AN IMMERSION MEDIUM 有权
标题翻译：检测和控制浸没介质供应的方法
公开(公告)号：US20150015942A1
公开(公告)日：2015-01-15
申请号：US14319246
申请日：2014-06-30
申请人： Carl Zeiss Microscopy GmbH
发明人： Ralf Steinmeyer , Reiner Mitzkus , Timo Rojahn
IPC分类号： G02B21/24 , G02B21/33
CPC分类号： G02B21/241 , G02B21/244 , G02B21/245 , G02B21/33
摘要： A method for detecting and controlling supply of an immersion medium into an immersion film region between the front lens of a microscope objective and a preparation, particularly in automated microscopes, using a light source which is reflected into the microscope beam path by means of a beam splitter. The state of the immersion film region is recorded and evaluated in a detection unit of the microscope or a detection unit of the autofocus device through an autofocus signal of the light source and/or a reflection of a modulation object arranged in the intermediate image plane of an incident light illumination, and is transmitted to a control unit for the purpose of a change or an error treatment of the immersion process.
摘要翻译：一种用于检测和控制浸入介质供给到显微镜物镜的前透镜和制剂之间的浸没介质的方法，特别是在自动显微镜中，使用通过光束反射到显微镜光束路径中的光源分离器通过光源的自动聚焦信号和/或布置在中间像平面中的调制对象的反射，在显微镜的检测单元或自动对焦装置的检测单元中记录和评估浸没膜区域的状态入射光照射，并且为了改变或者浸没处理的误差处理的目的被传送到控制单元。

8. 发明申请

US20160048967A1 METHOD AND DEVICE FOR DETERMINING A DISTANCE BETWEEN TWO OPTICAL BOUNDARY SURFACES WHICH ARE SPACED APART FROM EACH OTHER ALONG A FIRST DIRECTION 有权
标题翻译：用于确定两个光学边界表面之间的距离的方法和装置
公开(公告)号：US20160048967A1
公开(公告)日：2016-02-18
申请号：US14826355
申请日：2015-08-14
申请人： Carl Zeiss Microscopy GmbH
发明人： Reiner Mitzkus , Matthias Pospiech , Anke Vogelgsang
IPC分类号： G06T7/00 , G02B21/24 , G06K9/46 , G06T7/40 , G06T5/00
CPC分类号： G06T7/73 , G01B9/04 , G01B11/06 , G02B21/0016 , G02B21/244 , G02B21/245 , G02B21/367 , G06K9/4604 , G06T5/003 , G06T2207/10028 , G06T2207/10056 , G06T2207/10148
摘要： A method is provided for determining the distance between two optical boundary surfaces spaced apart from each other in a first direction. A first image is ascertained wherein the plane into which the pattern acquired coincides with a first of two optical boundary surfaces or has the smallest distance to the first optical boundary surface in a first direction. A position of the first image in the first direction is determined. A second image is ascertained wherein the plane into which the pattern acquired coincides with a second of two optical boundary surfaces or has the smallest distance to the second optical boundary surface in the first direction. The position of the second image in the first direction is determined. The distance is calculated by means of determined positions of the first and second image.
摘要翻译：提供了一种用于确定在第一方向上彼此间隔开的两个光学边界表面之间的距离的方法。确定第一图像，其中获取的图案的平面与两个光学边界表面中的第一个重合，或者在第一方向上具有与第一光学边界表面最小的距离。确定第一方向上的第一图像的位置。确定第二图像，其中获取的图案的平面与两个光学边界表面中的第二个相符，或者在第一方向上具有与第二光学边界表面最小的距离。确定第二图像在第一方向上的位置。距离通过第一和第二图像的确定位置来计算。

9. 发明申请

US20140226204A1 Optical Assembly and Light Microscope 审中-公开
标题翻译：光学组件和光学显微镜
公开(公告)号：US20140226204A1
公开(公告)日：2014-08-14
申请号：US14177339
申请日：2014-02-11
申请人： CARL ZEISS MICROSCOPY GMBH
发明人： Reiner Mitzkus , Heino Heise , Peter Westphal , Christoph Windolph
IPC分类号： G02B21/06 , G02B5/20
CPC分类号： G02B21/06 , G02B21/086 , G02B21/16 , G02B26/007
摘要： The invention relates to an optical assembly for spectral filtration of light, having a plurality of filters which are permeable to light of different spectral ranges, a filter selection mirror which can be moved for selectable deflection of light to different optical paths to the different filters, and an output mirror which can be moved to guide light coming from one of the filters to an optical path which is the same for all the filters. The optical assembly is characterized according to the invention in that in each case at least one stationary deflection optical system is provided for each of the optical paths to the different filters to guide light from the filter selection mirror to the respective filter and/or light from the respective filter to the output mirror, and the stationary deflection optical systems are arranged so that optical path lengths on the different optical paths from the filter selection mirror to the output mirror are equal. The invention further relates to a light microscope having an optical assembly according to the invention.
摘要翻译：本发明涉及一种用于光谱滤光的光学组件，其具有可透过不同光谱范围的光的多个滤光器，可移动的滤光器选择镜，用于将光可选择地偏转到不同光路到不同滤光片，以及可以移动以将来自一个滤光器的光引导到对于所有滤光器相同的光路的输出镜。根据本发明的光学组件的特征在于，在每种情况下，为每个到不同过滤器的光路提供至少一个固定偏转光学系统，以将来自过滤器选择镜的光引导到相应的过滤器和/或来自输出反射镜的相应滤光器和固定偏转光学系统被布置成使得从滤光器选择镜到输出镜的不同光路上的光路长度相等。本发明还涉及具有根据本发明的光学组件的光学显微镜。

10. 发明授权

US10359617B2 Microscope 有权
公开(公告)号：US10359617B2
公开(公告)日：2019-07-23
申请号：US15585828
申请日：2017-05-03
申请人： Carl Zeiss Microscopy GmbH
发明人： Matthias Pospiech , Reiner Mitzkus , Marzena Franek , Peter Westphal
IPC分类号： G02B21/36 , G02B21/34 , G02B21/24
摘要： A microscope includes a holder for holding a sample, an objective for imaging at least apart of a sample held by the holder, a detection module, a control unit for setting the focus position of the objective in a first direction for the recording by means of the detection module, and a focusing module for maintaining a set focus position of the objective. The focusing module includes the control unit, a second detector and first focusing optics with adjustable focal length. The focusing module is switchable into a focus-hold mode, wherein an intensity-modulated object is imaged into the sample via the first focusing optics and the objective, and an image of the imaged object is recorded by means of the second detector. The control unit holds the focus position of the objective on the set focus position, based upon the recording of the second detector.

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