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    • 9. 发明申请
    • Measurement Of Protease Activity In Post-Mortem Meat Samples
    • 后期肉类样品中蛋白酶活性的测定
    • US20100209943A1
    • 2010-08-19
    • US12682246
    • 2008-10-09
    • David GoldbergMartha Goldberg
    • David GoldbergMartha Goldberg
    • G01N33/573C12Q1/37B01D57/02
    • C12Q1/37A23L13/00
    • The present methods and systems relate to the placement on meat surfaces of a dye-linked protease substrate bonded in a defined location to a solid, porous support. Proteases in the meat hydrolyze the substrate, which then releases the dye, which diffuses away so that the extent of diffusion can be determined by imaging. Alternatively, the amount of dye released can be determined by its mobilization into a liquid medium. The present methods and systems also relates to determining the amount of hydrolyzed protein that is present in post-mortem meat samples. Agents which bond to hydrolyzed proteins, but not to unhydrolyzed proteins, are generated, wherein such agents comprise antibodies or aptamers. Such agents are then used in quantitative assays comprising ELISA tests or lateral flow tests.
    • 本发明的方法和系统涉及在与所定义的位置结合的固定多孔载体上的染料连接的蛋白酶底物的肉表面上的放置。 肉中的蛋白酶水解基质,然后释放染料,其扩散离开,使得通过成像可以确定扩散程度。 或者,释放的染料的量可以通过其移动到液体介质中来确定。 本发明的方法和系统还涉及确定在宰后肉样品中存在的水解蛋白质的量。 产生与水解的蛋白质结合而不是未水解的蛋白质的试剂,其中这些试剂包含抗体或适体。 然后将这样的试剂用于包括ELISA测试或侧流测试的定量测定中。