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    • 10. 发明授权
    • Maize metallothionein gene and promoter
    • 玉米金属硫蛋白基因和启动子
    • US06774282B1
    • 2004-08-10
    • US09520268
    • 2000-03-07
    • Steven W. Ritchie
    • Steven W. Ritchie
    • C12N1582
    • G06F3/147C07K14/825C12N15/8222C12N15/8227C12N15/8242C12N15/8271G09G2380/04
    • The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding, a metallothionein gene and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell with a nucleotide sequence operably linked to one of the root-preferred promoters of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence in a root-preferred manner from the transformed plant cell. Compositions and methods for expressing metallothionein genes in plants, plant cells and tissues are also provided. The compositions comprise nucleotide sequences encoding plant metallothionein. The sequences are useful in transforming plants for tissue-preferred or constitutive expression of metallothionein. Such sequences find use in modulating levels of metal ions in plants and plant tissues.
    • 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码金属硫蛋白基因的基因的根优选启动子和从其分离的序列的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括用可操作地连接到本发明的根优选启动子之一的核苷酸序列转化植物细胞,并且从转化的植物细胞再生以根优选方式表达核苷酸序列的稳定转化的植物。 还提供了在植物,植物细胞和组织中表达金属硫蛋白基因的组合物和方法。 组合物包含编码植物金属硫蛋白的核苷酸序列。 该序列可用于转化植物用于组织优选或组成型表达金属硫蛋白。 这些序列可用于调节植物和植物组织中金属离子的水平。