会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 10. 发明授权
    • Cry1F and Cry1Ac transgenic cotton lines and event-specific identification thereof
    • Cry1F和Cry1Ac转基因棉花品系及其事件特异性鉴定
    • US07179965B2
    • 2007-02-20
    • US10964838
    • 2004-10-13
    • Ping SongLaura Ann TaglianiJohn William Pellow
    • Ping SongLaura Ann TaglianiJohn William Pellow
    • A01H5/00A01H5/10C12N15/82
    • C12N15/8286C07K14/325C12Q1/6895Y02A40/162
    • This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.
    • 本发明涉及植物育种和植物对昆虫的保护。 更具体地,本发明包括棉花植物的新转化事件,其包含插入到棉花细胞基因组内的特定位点的如本文所述的一个或多个多核苷酸序列。 在非常优选的实施方案中,所述多核苷酸序列编码“堆积”Cry1F和Cry1Ac鳞翅目昆虫抑制蛋白。 然而,本发明包括如本文所述的具有单一cry1F或cry1Ac事件的植物。 此外,本发明涉及来自该转化事件的棉花植物和用于检测样品中事件的存在的测定法。 更具体地,本发明提供用于检测棉花中某些昆虫抗性事件的存在的DNA和相关测定法。 测定法基于插入棉花基因组的重组构建体的DNA序列和插入位点侧翼的基因组序列。 这些序列是唯一的。 基于这些插入和边界序列,生成事件特异性引物。 PCR分析表明,通过分析用这些事件特异性引物组产生的PCR扩增子,可以在不同棉花基因型中鉴定这些棉花品系。 因此,这些和其他相关程序可用于唯一地识别这些棉花线。 还提供了用于进行测定的试剂盒和条件。 这些材料和方法也可用于协助育种计划进一步发展棉花性状。