专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US11340234B2 Method for the determination of anti-drug antibodies against an effector function suppressed human or humanized drug antibody 有权
公开(公告)号：US11340234B2
公开(公告)日：2022-05-24
申请号：US15587730
申请日：2017-05-05
申请人： Hoffmann-La Roche Inc.
发明人： Pablo Umana , Uwe Wessels , Kay-Gunnar Stubenrauch
IPC分类号： G01N31/00 , G01N33/53 , G01N33/68 , G01N33/50
摘要： Herein is reported an anti-drug antibody immunoassay for the determination of the presence of an anti-drug antibody against an effector function suppressed human or humanized drug antibody in a sample comprising the incubation of a sample comprising mammalian blood serum with full length human Fcgamma receptor I or an Fc-region binding fragment thereof so that a complex between the anti-drug antibody against the effector function suppressed human or humanized drug antibody present in the sample and the human Fcgamma receptor I or the Fc-region binding fragment thereof forms, whereby the full length human Fcgamma receptor I or the Fc-region binding fragment thereof is conjugated to a detectable label, and the determination of the formed complex by the detectable label.

2. 发明申请

US20210364504A1 METHOD FOR DETERMINING THE AMOUNT OF A THERAPEUTIC ANTIBODY IN THE BRAIN 有权
公开(公告)号：US20210364504A1
公开(公告)日：2021-11-25
申请号：US17136289
申请日：2020-12-29
申请人： Hoffmann-La Roche Inc.
发明人： Martin Schaefer , Sylvia Rottach , Gregor Jordan , Kay-Gunnar Stubenrauch , Roland Staack , Kevin Brady
IPC分类号： G01N33/543 , C07K16/28 , C07K16/40
摘要： Herein is reported a method for determining the concentration of a therapeutic antibody in a tissue of an experimental animal to whom the therapeutic antibody had been administered, wherein the interference from residual blood in a tissue sample of the experimental animal, which is used for determining the concentration of the therapeutic antibody in said tissue, is reduced, wherein the concentration of the therapeutic antibody in the tissue of the experimental animal is calculated with the following formula: C tmAb , tissue = C tmAb , tissue , det . C tissue , sample - C refmAb , tissue , det . C tissue , sample C refmAb , plasma . det . * C tmAb , plasma , det . wherein CtmAb,tissue,det.=obtained by determining the concentration of the therapeutic antibody in the tissue sample of the experimental animal, CtmAb,plasma,det.=obtained by determining the concentration of the therapeutic antibody in a blood sample of the experimental animal directly prior to taking the tissue sample, CrefmAb,tissue,det.=obtained by determining the concentration of the inert reference antibody in the tissue sample of the experimental animal, CrefmAb,plasma,det.=obtained by determining the concentration of an inert reference antibody in the blood sample of the experimental animal directly prior to taking the tissue sample, Ctissue,sample=obtained by determining the tissue concentration in the tissue sample, whereby the inert reference antibody does not penetrate into said tissue, whereby the inert reference antibody is administered 2 to 10 minutes prior to obtaining the tissue and blood sample.

3. 发明申请

US20180364251A1 Immunoassay for the determination of Fc-region modified antibodies 审中-公开
公开(公告)号：US20180364251A1
公开(公告)日：2018-12-20
申请号：US15988939
申请日：2018-05-24
申请人： Hoffmann-La Roche Inc.
发明人： Kay-Gunnar Stubenrauch
IPC分类号： G01N33/68 , G01N33/543
摘要： Herein is reported a method for the determination of the amount of a bivalent antibody in a serum or plasma sample obtained from a non-human experimental animal, whereby the antibody comprises one or more mutations in the Fc-region compared to the corresponding wild-type Fc-region that has a sequence of SEQ ID NO: 01, 02, or 03, wherein the method comprises the following steps a) immobilizing a non-antibody polypeptide to which more than one copy of the antigen of the antibody is covalently conjugated on a solid surface, b) incubating the immobilized antigen with the sample to form an immobilized antigen-antibody complex, c) incubating the immobilized antigen-antibody complex with the antigen conjugated to a detectable label to form an immobilized ternary complex, and d) determining the amount of the antibody by determining the amount of the detectable label in the immobilized ternary complex.

4. 发明授权

US09945869B2 Method for the detection of a binding partner of a multispecific binder 有权
公开(公告)号：US09945869B2
公开(公告)日：2018-04-17
申请号：US14449437
申请日：2014-08-01
申请人： Hoffmann-La Roche Inc.
发明人： Kay-Gunnar Stubenrauch , Uwe Wessels
IPC分类号： G01N33/53 , G01N33/68 , G01N33/543
CPC分类号： G01N33/6854 , G01N33/54306 , G01N2333/00
摘要： Herein is reported a method for the detection of free antigen of a multispecific antibody in a sample, whereby the antigen to be detected can be specifically bound by a first binding site of the multispecific antibody, comprising the step of incubating a sample comprising free antigen and multispecific antibody with an anti-idiotypic antibody, which specifically binds to a second binding specificity of the bispecific antibody, which is different from the first binding specificity, whereby the anti-idiotypic antibody is bound to a solid phase.

5. 发明授权

US09933433B2 Anti-drug antibody assay 有权
公开(公告)号：US09933433B2
公开(公告)日：2018-04-03
申请号：US14452122
申请日：2014-08-05
申请人： Hoffmann-La Roche Inc.
发明人： Ulrich Essig , Kay-Gunnar Stubenrauch , Rudolf Vogel , Uwe Wessels
IPC分类号： G01N33/68 , C07K16/42 , C07K16/06
CPC分类号： G01N33/6854 , C07K16/065 , C07K16/42 , C07K16/4283 , C07K2317/21 , C07K2317/32 , C07K2317/33 , C07K2317/52
摘要： The invention provides an antibody binding specifically to Cynomolgus IgG characterized by not binding to Human IgG, and a method for the immunological determination of an immune complex (DA/ADA complex) of a drug antibody (DA) and an antibody against said drug antibody (anti-drug antibody, ADA) in a sample of a monkey species using a double antigen bridging immunoassay.

6. 发明申请

US20160077100A1 Specific Detection of Rat Antibodies in Mouse Serum 审中-公开
标题翻译：小鼠血清中大鼠抗体的特异性检测
公开(公告)号：US20160077100A1
公开(公告)日：2016-03-17
申请号：US14858449
申请日：2015-09-18
申请人： Hoffmann-La Roche Inc.
发明人： Kay-Gunnar Stubenrauch , Uwe Wessels
IPC分类号： G01N33/68 , G01N33/94
CPC分类号： G01N33/686 , G01N33/6854 , G01N33/94
摘要： One aspect as reported herein is a method for detecting a rat antibody in a serum or plasma sample (obtained) from a mouse comprising the steps of a) providing the sample to be analyzed, b) incubating said serum or plasma sample with an antibody that specifically binds to rat IgG and that does not specifically bind to mouse IgG, wherein the antibody is i) a mixture of an antibody binding to rat kappa light chain and an antibody binding to rat lambda light chain, or ii) a mixture of an antibody binding to rat IgG1 with an avidity of 4.1×1010 M−1 or more, an antibody binding to rat IgG2a with an avidity of 8.6×109 M−1 or more, an antibody binding to rat IgG2b with an avidity of 6.4×1010 M−1 or more and an antibody binding to rat IgG2c with an avidity of 9.5×1010 M−1 or more, c) optionally incubating said sample with a reagent appropriate for the selective detection of total, active or antigen-bound rat antibody, and d) correlating the complex formed in (b) or (c) to the concentration of the rat antibody in the sample.
摘要翻译：本文报道的一个方面是从小鼠检测血清或血浆样品（获得）中的大鼠抗体的方法，包括以下步骤：a）提供待分析的样品，b）将所述血清或血浆样品与抗体特异性结合大鼠IgG并且不特异性结合小鼠IgG，其中抗体是i）结合大鼠κ轻链的抗体与结合大鼠λ轻链的抗体的混合物，或ii）抗体的混合物与亲和性为4.1×10 10 M -1以上的大鼠IgG1结合，抗亲和力为8.6×10 9 M -1以上的与大鼠IgG2a结合的抗体，结合大鼠IgG2b的亲和力为6.4×10 10 M -1以上，以及抗亲和度为9.5×10 10 M -1以上的与大鼠IgG2c结合的抗体，c）任选地用适合选择性检测总的，活性或抗原结合的大鼠抗体的试剂培养所述样品，以及 d）将（b）或（c）中形成的复合物与可见物相关联样品中大鼠抗体的浓度。

7. 发明授权

US10761097B2 Method for the detection of a binding partner of a multispecific binder 有权
公开(公告)号：US10761097B2
公开(公告)日：2020-09-01
申请号：US15923773
申请日：2018-03-16
申请人： Hoffmann-La Roche Inc.
发明人： Kay-Gunnar Stubenrauch , Uwe Wessels
IPC分类号： G01N33/68 , G01N33/543 , G01N33/53
摘要： Herein is reported a method for the detection of free antigen of a multispecific antibody in a sample, whereby the antigen to be detected can be specifically bound by a first binding site of the multispecific antibody, comprising the step of incubating a sample comprising free antigen and multispecific antibody with an anti-idiotypic antibody, which specifically binds to a second binding specificity of the bispecific antibody, which is different from the first binding specificity, whereby the anti-idiotypic antibody is bound to a solid phase.

8. 发明申请

US20180346602A1 ANTI-VARIANT FC-REGION ANTIBODIES AND METHOD OF USE 审中-公开
公开(公告)号：US20180346602A1
公开(公告)日：2018-12-06
申请号：US15962429
申请日：2018-04-25
申请人： Hoffmann-La Roche Inc.
发明人： Frank Kowalewsky , Mirko Ritter , Kay-Gunnar Stubenrauch , Uwe Wessels
IPC分类号： C07K16/46 , G01N33/53
CPC分类号： C07K16/22 , C07K16/00 , C07K16/283 , C07K16/2854 , C07K16/2863 , C07K16/42 , C07K2317/524 , C07K2317/526
摘要： The invention provides anti-variant Fc-region antibodies which specifically bind to an antibody that has the mutation P329G or the mutations P329G/L234A/L235A or the mutations I253A/H310A/H435A in the Fc-region, and methods of using the same.

9. 发明授权

US09547010B2 Anti-idiotype antibody against an antibody against the amyloid β peptide 有权
标题翻译：针对淀粉样蛋白β肽抗体的抗独特型抗体
公开(公告)号：US09547010B2
公开(公告)日：2017-01-17
申请号：US13960893
申请日：2013-08-07
申请人： Hoffmann-La Roche Inc.
发明人： Ulrich Essig , Wolfgang Hoesel , Kay-Gunnar Stubenrauch , Rudolf Vogel
IPC分类号： G01N33/532 , C07K16/42 , G01N33/68
CPC分类号： G01N33/686 , C07K16/4241 , C07K2317/56 , C07K2317/565 , G01N2333/4709 , G01N2469/20
摘要： The present invention is directed to an anti-idiotype antibody binding to the complementary determining region of an antibody against the amyloid β peptide. In one embodiment said antibody binds to the same epitope or an overlapping epitope as the antibody obtainable from the cell line DSM ACC2939. Also reported is an immunoassay for the determination of an antibody against the amyloid β peptide and for determination of an anti-idiotype antibody binding to an antibody against the amyloid β peptide.
摘要翻译：本发明涉及与抗Aβ淀粉样蛋白抗体的互补决定区结合的抗独特型抗体。在一个实施方案中，所述抗体结合与从细胞系DSM ACC2939获得的抗体相同的表位或重叠表位。还报道了用于测定针对淀粉样蛋白β肽的抗体的免疫测定和用于测定与针对淀粉样蛋白β肽的抗体结合的抗独特型抗体。

10. 发明申请

US20150253338A1 ANTI-DRUG ANTIBODY ASSAY 审中-公开
标题翻译：抗药物抗体测定
公开(公告)号：US20150253338A1
公开(公告)日：2015-09-10
申请号：US14714593
申请日：2015-05-18
申请人： Hoffmann-La Roche Inc.
发明人： Wolfgang Hoesel , Kay-Gunnar Stubenrauch , Rudolf Vogel
IPC分类号： G01N33/68
CPC分类号： G01N33/6854 , G01N33/54353 , G01N33/54393 , G01N33/686
摘要： The invention provides a method for the immunological determination of an antibody against a drug antibody in a sample using a double antigen bridging immunoassay comprising a capture drug antibody and a tracer drug antibody, characterized in that the capture drug antibody is a mixture of said drug antibody conjugated to the solid phase at at least two different antibody sites and the tracer drug antibody is a mixture of said drug antibody conjugated to the detectable label at at least two different antibody sites.
摘要翻译：本发明提供了使用包含捕获药物抗体和示踪药物抗体的双抗原桥接免疫测定免疫测定样品中抗体的抗体的方法，其特征在于捕获药物抗体是所述药物抗体的混合物在至少两个不同抗体位点与固相缀合，示踪药物抗体是在至少两个不同抗体位点缀合至可检测标记的所述药物抗体的混合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式