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1. 发明申请

US20090156419A1 HYBRIDIZATION METHODS USING NATURAL BASE MISMATCHES 有权
标题翻译：使用自然基因杂交的杂交方法
公开(公告)号：US20090156419A1
公开(公告)日：2009-06-18
申请号：US12162286
申请日：2007-02-06
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
IPC分类号： C40B30/04
CPC分类号： C12Q1/6881 , C12Q1/6832 , C12Q2600/156 , C12Q2535/131 , C12Q2527/107
摘要： The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
摘要翻译：本发明提供使用包含天然存在的核苷酸碱基的修饰的寡核苷酸探针的改进的核酸杂交方法。除了由含有序列变异的变体核酸靶产生的任何错配之外，修饰寡核苷酸中至少一个核苷酸相对于对照核酸人为错配。将人工错配和序列变异位置在寡核苷酸上彼此分开6至9个核苷酸位置。

2. 发明申请

US20090143245A1 MICROARRAYS FOR GENOTYPING AND METHODS OF USE 审中-公开
标题翻译：用于基因组的微阵列和使用方法
公开(公告)号：US20090143245A1
公开(公告)日：2009-06-04
申请号：US12093504
申请日：2006-02-20
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Chuanzan Zhao , Li Li , Gengxin Lan , Tao Guo , Bin Cai , Wanli Xing , Yuxiang Zhou , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Chuanzan Zhao , Li Li , Gengxin Lan , Tao Guo , Bin Cai , Wanli Xing , Yuxiang Zhou , Jing Cheng
IPC分类号： C40B30/10 , C40B40/08 , C40B50/06
CPC分类号： C12Q1/6881 , C12Q1/6837 , C12Q2600/156
摘要： The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided.
摘要翻译：本发明提供了用于检测多个核酸样品中多态性位点处的基因型的微阵列，其包含衍生自样品的第一组核酸片段和衍生自多个固定在其上的参考文献的第二组核酸片段。本发明还提供了一种微阵列，其包含固定在微阵列表面上的一组核酸片段，其中通过不对称PCR扩增扩增含有多态性的样品中的区域，来从样品衍生出核酸片段。还提供了使用和制造微阵列的方法。

3. 发明授权

US09200323B2 Hybridization methods using natural base mismatches 有权
标题翻译：使用天然碱基错配的杂交方法
公开(公告)号：US09200323B2
公开(公告)日：2015-12-01
申请号：US12162286
申请日：2007-02-06
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6832 , C12Q2600/156 , C12Q2535/131 , C12Q2527/107
摘要： The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
摘要翻译：本发明提供使用包含天然存在的核苷酸碱基的修饰的寡核苷酸探针的改进的核酸杂交方法。除了由含有序列变异的变体核酸靶产生的任何错配之外，修饰寡核苷酸中至少一个核苷酸相对于对照核酸人为错配。将人工错配和序列变异位置在寡核苷酸上彼此分开6至9个核苷酸位置。

4. 发明授权

US5833856A Process for biologically removing phosphorus and nitrogen from wastewater by controlling carbohydrate content therein 失效
标题翻译：通过控制碳水化合物含量从废水中生物去除磷和氮的方法
公开(公告)号：US5833856A
公开(公告)日：1998-11-10
申请号：US896662
申请日：1997-07-18
申请人： Yanhua Liu , Zaoyan Yang
发明人： Yanhua Liu , Zaoyan Yang
IPC分类号： C02F3/30
CPC分类号： C02F3/308
摘要： The present invention relates to a process and a treatment system for biologically removing phosphorus and nitrogen from wastewater. In the anaerobic contact and settlement zone 1, wastewater 7 is first mixed with the returned activated sludge 8 to form a mixed liquor which is then allowed to settle in zone 1, and organic matters present in influent are mostly taken up by the sludge. The supernatant from zone 1 is passed through an aerobic reaction zone 2, furnished with packings, for complete nitrification; the settled sludge from zone 1 is hold in an anaerobic zone 3 for a sufficient period of time for controlling the sludge carbohydrate content to low levels in order to enlarge the sludge capacity for phosphorus removal because the extended anaerobic sludge degradation in zone 3 is very effective in controlling the sludge carbohydrate content to low levels. The sludge from zone 3 is then allowed to be remixed with the nitrified supernatant from zone 2 in zone 4, where the products of the extended anaerobic reaction allowed very high denitrification rates and efficiencies. The aerobic zone 5 is supplemented to complete phosphorus uptake by the activated sludge, in which the dissolved oxygen (DO) level is controlled at relatively low levels in order to limit the aerobic synthesis of sludge carbohydrate. The aerated mixed liquor is then transferred into a final clarifier where settled sludge is separated from purified supernatant. At least a portion of the settled sludge is recycled back to zone 1 to be mixed with raw wastewater, while a portion of the settled sludge is wasted.
摘要翻译：本发明涉及从废水中生物去除磷和氮的方法和处理系统。在厌氧接触和沉降区1中，首先将废水7与返回的活性污泥8混合，形成混合液，然后使其在区域1中沉降，并且存在于流入物中的有机物主要被污泥吸收。来自区域1的上清液通过需氧反应区2，配有填料，用于完全硝化; 来自区域1的沉降污泥在厌氧区3中保持足够的时间，以便将污泥碳水化合物含量控制在低水平，以便扩大污泥的除磷能力，因为区域3中延长的厌氧污泥降解非常有效将污泥碳水化合物含量控制在较低水平。然后将来自区域3的污泥与区域4中的区域2的硝化上清液重新混合，其中延长厌氧反应的产物允许非常高的脱氮率和效率。补充好氧区5以通过活性污泥完成磷吸收，其中将溶解氧（DO）水平控制在相对低的水平，以限制污泥碳水化合物的需氧合成。然后将充气混合液转移到最终的澄清器中，其中沉淀的污泥与纯化的上清液分离。至少一部分沉淀的污泥被循环回到区域1以与原始废水混合，而一部分沉降的污泥被浪费。

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