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1. 发明申请

US20090156419A1 HYBRIDIZATION METHODS USING NATURAL BASE MISMATCHES 有权
标题翻译：使用自然基因杂交的杂交方法
公开(公告)号：US20090156419A1
公开(公告)日：2009-06-18
申请号：US12162286
申请日：2007-02-06
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
IPC分类号： C40B30/04
CPC分类号： C12Q1/6881 , C12Q1/6832 , C12Q2600/156 , C12Q2535/131 , C12Q2527/107
摘要： The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
摘要翻译：本发明提供使用包含天然存在的核苷酸碱基的修饰的寡核苷酸探针的改进的核酸杂交方法。除了由含有序列变异的变体核酸靶产生的任何错配之外，修饰寡核苷酸中至少一个核苷酸相对于对照核酸人为错配。将人工错配和序列变异位置在寡核苷酸上彼此分开6至9个核苷酸位置。

2. 发明授权

US09200323B2 Hybridization methods using natural base mismatches 有权
标题翻译：使用天然碱基错配的杂交方法
公开(公告)号：US09200323B2
公开(公告)日：2015-12-01
申请号：US12162286
申请日：2007-02-06
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Li Li , Chuanzan Zhao , Gengxin Lan , Tao Guo , Bin Cai , Jing Cheng
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6832 , C12Q2600/156 , C12Q2535/131 , C12Q2527/107
摘要： The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
摘要翻译：本发明提供使用包含天然存在的核苷酸碱基的修饰的寡核苷酸探针的改进的核酸杂交方法。除了由含有序列变异的变体核酸靶产生的任何错配之外，修饰寡核苷酸中至少一个核苷酸相对于对照核酸人为错配。将人工错配和序列变异位置在寡核苷酸上彼此分开6至9个核苷酸位置。

3. 发明申请

US20090143245A1 MICROARRAYS FOR GENOTYPING AND METHODS OF USE 审中-公开
标题翻译：用于基因组的微阵列和使用方法
公开(公告)号：US20090143245A1
公开(公告)日：2009-06-04
申请号：US12093504
申请日：2006-02-20
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Chuanzan Zhao , Li Li , Gengxin Lan , Tao Guo , Bin Cai , Wanli Xing , Yuxiang Zhou , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Chuanzan Zhao , Li Li , Gengxin Lan , Tao Guo , Bin Cai , Wanli Xing , Yuxiang Zhou , Jing Cheng
IPC分类号： C40B30/10 , C40B40/08 , C40B50/06
CPC分类号： C12Q1/6881 , C12Q1/6837 , C12Q2600/156
摘要： The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided.
摘要翻译：本发明提供了用于检测多个核酸样品中多态性位点处的基因型的微阵列，其包含衍生自样品的第一组核酸片段和衍生自多个固定在其上的参考文献的第二组核酸片段。本发明还提供了一种微阵列，其包含固定在微阵列表面上的一组核酸片段，其中通过不对称PCR扩增扩增含有多态性的样品中的区域，来从样品衍生出核酸片段。还提供了使用和制造微阵列的方法。

4. 发明授权

US07718362B2 DNA chip based genetic typing 有权
标题翻译：基于DNA芯片的遗传分型
公开(公告)号：US07718362B2
公开(公告)日：2010-05-18
申请号：US10562803
申请日：2003-07-18
申请人： Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Yiming Zhou , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
发明人： Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Yiming Zhou , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34
CPC分类号： C12Q1/6837
摘要： This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.
摘要翻译：本发明一般涉及核酸分析领域。特别地，本发明提供了一种用于对目标基因进行分型的方法，特别地，使用包含适合用于核酸杂交的载体的芯片，其上固定有与所述靶核苷酸序列互补的寡核苷酸探针和至少一种以下寡核苷酸对照探针：阳性对照探针，阴性对照探针，杂交对照探针和固定化对照探针。还提供了用于分型HLA靶基因的寡核苷酸探针或探针阵列。

5. 发明申请

US20070134661A1 Dna chip based genetic typing 有权
标题翻译：基于Dna芯片的遗传分型
公开(公告)号：US20070134661A1
公开(公告)日：2007-06-14
申请号：US10562803
申请日：2003-07-18
申请人： Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
发明人： Huafang Gao , Xuemei Ma , Chi Zhang , Qian Chen , Dong Wang , Yizhe Zhang , Yue Tian , Rui Zhang , Gengxin Lan , Yuxiang Zhou , Jing Cheng
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： C12Q1/6837
摘要： This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.
摘要翻译：本发明一般涉及核酸分析领域。特别地，本发明提供了一种用于对目标基因进行分型的方法，特别地，使用包含适合用于核酸杂交的载体的芯片，其上固定有与所述靶核苷酸序列互补的寡核苷酸探针和至少一种以下寡核苷酸对照探针：阳性对照探针，阴性对照探针，杂交对照探针和固定化对照探针。还提供了用于分型HLA靶基因的寡核苷酸探针或探针阵列。

6. 发明授权

US09677133B2 Biological chip hybridization system 有权
公开(公告)号：US09677133B2
公开(公告)日：2017-06-13
申请号：US14006540
申请日：2012-02-10
申请人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
发明人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
IPC分类号： C12Q1/68 , G01N33/50 , G01N33/543 , B01L3/00 , B01L7/00 , G01N35/00
CPC分类号： C12Q1/6876 , B01L3/5027 , B01L7/52 , B01L2300/0636 , B01L2300/0816 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0622 , G01N33/543 , G01N2035/00158
摘要： A hybridization system is provided, which comprises a biological chip having a substrate (1) with a probe dot matrix (3) and a cover plate (2) with at least two through-holes (4), where a hybrid chamber (5) is formed between the substrate (1) and the cover plate (2), at least two fluid channels (6) are interconnected respectively with the hybrid chamber (5) by the two through-holes (4), and a fluid control device is interconnected with the fluid channels (6). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels (6) and the chamber (5) to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.

7. 发明申请

US20140011707A1 BIOLOGICAL CHIP HYBRIDIZATION SYSTEM 有权
标题翻译：生物芯片混合系统
公开(公告)号：US20140011707A1
公开(公告)日：2014-01-09
申请号：US14006540
申请日：2012-02-10
申请人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
发明人： Jiaming Ye , Pinhong Wang , Guoqing Wang , Liqing Duan , Kaijun Zhao , Jinxiu Zhang , Xiumei Song , Dong Wang , Huafang Gao , Wanli Xing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , B01L3/5027 , B01L7/52 , B01L2300/0636 , B01L2300/0816 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0622 , G01N33/543 , G01N2035/00158
摘要： A hybridization system is provided, which comprises a biological chip having a substrate (1) with a probe dot matrix (3) and a cover plate (2) with at least two through-holes (4), where a hybrid chamber (5) is formed between the substrate (1) and the cover plate (2), at least two fluid channels (6) are interconnected respectively with the hybrid chamber (5) by the two through-holes (4), and a fluid control device is interconnected with the fluid channels (6). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels (6) and the chamber (5) to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.
摘要翻译：提供了一种杂交系统，其包括具有带有探针点阵（3）的基底（1）和具有至少两个通孔（4）的盖板（2）的生物芯片，其中混合室（5）形成在基板（1）和盖板（2）之间，至少两个流体通道（6）分别通过两个通孔（4）与混合室（5）相互连接，并且流体控制装置与流体通道（6）互连。生物芯片杂交系统集成了杂交，清洁和干燥功能，使用由流体控制装置提供的电力作为促进液体流动的驱动力，用于在流体通道（6）和腔室（5）中自动往复流动，以实现动态杂交，从而提高混合效率和均匀性，实现自动控制。

8. 发明申请

US20080267828A1 Micro-Volume Liquid Ejection System 有权
标题翻译：微量液体喷射系统
公开(公告)号：US20080267828A1
公开(公告)日：2008-10-30
申请号：US11666032
申请日：2004-11-22
申请人： Dong Wang , Kun Zou , Jianxing Ye , Yelei Sun , Hui Zhu , Xianhua Wang , Jing Cheng
发明人： Dong Wang , Kun Zou , Jianxing Ye , Yelei Sun , Hui Zhu , Xianhua Wang , Jing Cheng
IPC分类号： B65D83/00
CPC分类号： B01L3/0265 , B01L2400/0487 , B01L2400/0666 , Y10T436/2575
摘要： The present invention relates to a micro-volume liquid ejection system, including an air pressure module, a micro-ejection unit which is connected with the air pressure module by means of pipes, and a control circuit which is connected with the air pressure module and the micro-ejection unit respectively. In the present invention, due to air is used as the pressure medium, on one hand, as the sample does not contact with the pressure regulating module, the efficiency of cleaning process is improved, on the other hand, the volume of the sample needed in the sample ejecting process is only equal to the cavity-dimension of the micro-ejection unit by not need to fill the whole pipe with liquid. During the sample ejecting process, it is not need to regulate the pressure. After the sample ejecting is finished, the sample can be put into its original place, so as to greatly save the sample. The present invention is connected with the manipulator, so as to sample from the sample plate, eject sample and clean the pipes automatically, and it can also dispense a multiplicity of samples to micro-array substrate conveniently. The present invention can be used for transferring or dispensing the micro volume liquid of nL grade and L grade widely, and all kinds of the micro-volume liquid including biological liquid.
摘要翻译：微体积液体喷射系统技术领域本发明涉及一种微体积液体喷射系统，包括空气压力模块，通过管道与空气压力模块连接的微型喷射单元，以及与气压模块连接的控制电路和微型喷射单元。在本发明中，由于空气被用作压力介质，一方面由于样品不与压力调节模块接触，因此提高了清洗过程的效率，另一方面，样品的体积需要在样品喷射过程中，仅通过不需要用整个管道填充液体就等于微喷射单元的腔体尺寸。在样品喷射过程中，不需要调节压力。样品喷射完成后，样品可以放在原来的位置，大大节省样品。本发明与机械手连接，从样品板上取样，自动排出样品并清洗管道，方便地将多个样品分配给微阵列基板。本发明可广泛用于转移或分配nL级和L级微量液体，以及各种微体积液体，包括生物液体。

9. 发明申请

US20070054273A1 Microarray devices having controllable reaction volume 有权
标题翻译：具有可控反应体积的微阵列装置
公开(公告)号：US20070054273A1
公开(公告)日：2007-03-08
申请号：US10566329
申请日：2003-08-13
申请人： Feijun Xian , Wanli Xing , Jing Cheng , Dong Liang , Liang Zhang , Dong Wang
发明人： Feijun Xian , Wanli Xing , Jing Cheng , Dong Liang , Liang Zhang , Dong Wang
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L3/50853 , B01J2219/00281 , B01J2219/00596 , B01J2219/00659 , B01L9/52 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , C12Q1/6837 , C40B60/14 , G01N33/54366
摘要： This invention relates generally to the field of microarray chips and uses thereof. In particular, the invention provides a microarray reaction device that can be used in assaying the interaction between various moieties, e.g., nucleic acids, immunoreactions involving proteins, interactions between a protein and a nucleic acid, a ligand-receptor interaction, and small molecule and protein or nucleic acid interactions, etc. Articles of manufacture and kits comprising the microarray reaction device and assaying methods using the microarray reaction device are also provided.
摘要翻译：本发明一般涉及微阵列芯片领域及其用途。特别地，本发明提供一种微阵列反应装置，其可用于测定各种部分之间的相互作用，例如核酸，涉及蛋白质的免疫反应，蛋白质与核酸之间的相互作用，配体 - 受体相互作用和小分子，以及蛋白质或核酸相互作用等。还提供了包含微阵列反应装置的生产制品和试剂盒以及使用微阵列反应装置的测定方法。

10. 发明申请

US20090301231A1 Microvalve Controlled Precision Fluid Dispensing Apparatus with a Self-Purging Feature and Method for use 有权
标题翻译：具有自清洗特征的微型阀控精密液体分配装置及其使用方法
公开(公告)号：US20090301231A1
公开(公告)日：2009-12-10
申请号：US11991723
申请日：2006-01-13
申请人： Dong Wang , Kun Zou , Jing Cheng
发明人： Dong Wang , Kun Zou , Jing Cheng
IPC分类号： G01N1/14 , G01N35/10 , G01F11/28
CPC分类号： B41J2/17596 , B01J2219/0036 , B01J2219/00364 , B01L3/0265 , B01L2400/0478 , B01L2400/0622 , B41J2/175 , G01N2035/00247 , G01N2035/1041 , Y10T436/2575
摘要： An apparatus and methods for the precise, repeatable dispensing of small, sample fluid volumes, especially as related to the printing of microarrays for biological and/or chemical testing. A pressure tuning module meters fluid volumes for aspiration and dispensation and builds pressure for fluid dispensation, in conjunction with a microvalve that precisely controls the volume of the fluid dispensed under the built pressure. A pressure source can be switched in line to efficiently purge the apparatus of residual sample fluid. A working fluid can be optionally aspirated into the system, prior to aspirating the sample fluid, in order to maximize sample fluid recovery.
摘要翻译：用于精确，可重复分配小的样品流体体积的装置和方法，特别是与用于生物和/或化学测试的微阵列的印刷相关。压力调节模块计量用于抽吸和分配的流体体积，并建立用于流体分配的压力，以及精确控制在内置压力下分配的流体的体积的微型阀。压力源可以排成一行，以有效地吹扫残留样品液的装置。在抽吸样品流体之前，可以将工作流体任选地吸入系统，以使样品液体回收最大化。

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