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    • 7. 发明授权
    • Immobilized lipases on a dry, porous particulate hydrophobic support and
containing a non-ionic surfactant
    • 固定的脂肪酶在干燥,多孔的颗粒疏水性载体上并含有非离子表面活性剂
    • US5773266A
    • 1998-06-30
    • US545818
    • 1995-11-09
    • John Anthony BosleyStephen Raymond Moore
    • John Anthony BosleyStephen Raymond Moore
    • C12N11/00C12N11/08C12N11/14C12N11/16C12P7/64C12N9/20
    • C12N11/00C12N11/08C12N11/14
    • Immobilized lipases are prepared containing lipase bound to a dry, porous particulate hydrophobic support having an average pore size of 0.05-5 .mu.m. Preparation is carried out by contacting the hydrophobic support with an aqueous solution of the lipase and with a non-ionic surfactant containing at least one fatty acid and having an HLB-value of at least 8 to provide a supported lipase, washing the supported lipase and drying the washed supported lipase to obtain the immobilized lipase. The lipase may be obtained from Candida Antarctica .beta., Rhizomucor miehei,Humicolo or Candida Rosa, and be a lipase specific for esterification of fatty acids with alcohol, 1,3-specific or randomizing transesterification lipase or lipase specific for the hydrolysis of partial glycerides, esters or triglycerides. The surfactant may be present in the aqueous solution of lipase in a concentration of 0.01-10 wt. %, and preferred surfactants are a polyoxyethylene sorbitan fatty acid ester and a polyglycerol fatty acid ester. The support may be a aliphatic olefinic polymer such as polyethylene or polypropylene, a homo- or copolymer of styrene or a blend thereof or a pre-treated inorganic support. The immobilized lipases are used for hydrolysis of triglycerides, diglycerides or esters or for the esterification or transesterification of fatty acids, diglycerides or triglycerides.
    • PCT No.PCT / EP94 / 01308 371日期:1995年11月9日 102(e)1995年11月9日PCT PCT 1994年4月22日PCT公布。 出版物WO94 / 28118 日期1994年12月8日制备含有脂肪酶的脂肪酶,该脂肪酶与平均孔径为0.05-5μm的干燥,多孔的颗粒状疏水性载体结合。 通过使疏水性载体与脂肪酶的水溶液和含有至少一种脂肪酸并且具有至少8的HLB值的非离子表面活性剂接触以提供负载的脂肪酶,洗涤负载的脂肪酶和 干燥经洗涤的负载型脂肪酶,得到固定化的脂肪酶。 该脂肪酶可以从南极假丝酵母(Candida)南极洲(Candida)南极的β,Rhizomucor miehei,Humicolo或者假丝酵母(Rosa)获得,并且是脂肪酸与醇酯化的脂肪酶,1,3-特异性或随机的酯交换脂肪酶或特异于部分甘油酯的水解的脂肪酶, 酯或甘油三酯。 表面活性剂可以以0.01-10重量%的浓度存在于脂肪酶水溶液中。 %,优选的表面活性剂是聚氧乙烯山梨糖醇酐脂肪酸酯和聚甘油脂肪酸酯。 载体可以是脂族烯烃聚合物如聚乙烯或聚丙烯,苯乙烯或其共混物的均聚物或共聚物或预处理的无机载体。 固定的脂肪酶用于甘油三酯,甘油二酯或酯的水解,或用于脂肪酸,甘油二酯或甘油三酯的酯化或酯交换。