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1. 发明授权

US10604735B2 Yeast strain having improved capability for fermenting xylose in the presence of acetic acid 有权
公开(公告)号：US10604735B2
公开(公告)日：2020-03-31
申请号：US15568188
申请日：2016-04-27
申请人： LESAFFRE ET COMPAGNIE
发明人： Jean-Michel Bavouzet , Thomas Desfougeres , Georges Pignede , Jennifer Techel
IPC分类号： C12N1/14 , C12N9/92 , C12P7/06 , C12P7/04 , C12N1/18 , C12N1/36 , C12R1/865 , C12N1/16 , C12P7/10
摘要： The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.

2. 发明授权

US10273447B2 Pentose-fermenting strain with optimized propagation 有权
公开(公告)号：US10273447B2
公开(公告)日：2019-04-30
申请号：US15116756
申请日：2015-02-16
申请人： LESAFFRE et COMPAGNIE
发明人： Thomas Desfougeres , Georges Pignede , Jennifer Techel
IPC分类号： C12N1/36 , C12N15/81 , C12R1/865 , C12P7/10 , C12N9/12 , C12N9/92
摘要： The present invention relates to a method for obtaining a strain capable of efficiently propagating in a low nutritive potential medium, capable of metabolizing pentoses and of resisting fermentation inhibitors, comprising the following steps; a) growth of a strain of recombinant yeast with a strain of wild yeast lacking any impairments, the recombinant yeast strain comprising at least one copy of an exogenous gene of xylose isomerase and at least one additional copy of a gene of D-xylulokinase included in the genome and linked to a single sexual characteristic of the strain, b) at least two cycles of genome shuffling by sporulation and or random hybridization, c) selection of the population obtained in step b) according to a suitability criterion of the strains to metabolize xylose, d) selection of the population obtained in step c) according to a suitability criterion of the strains to grow in a Pref type medium, a medium with low nutritive value. The invention also relates to a cell, a yeast or a strain of recombinant yeast obtained according to the method, and to the use of such a cell for the production of bioethanol.

3. 发明授权

US11186814B2 Yeast strain having improved capability for fermenting xylose in the presence of acetic acid 有权
公开(公告)号：US11186814B2
公开(公告)日：2021-11-30
申请号：US16793372
申请日：2020-02-18
申请人： LESAFFRE et COMPAGNIE
发明人： Jean-Michel Bavouzet , Thomas Desfougeres , Georges Pignede , Jennifer Techel
IPC分类号： C12N1/14 , C12N9/92 , C12N7/04 , C12N1/18 , C12N1/36 , C12N1/16 , C12P7/10 , C12P7/06 , C12R1/865
摘要： The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.

4. 发明申请

US20200208098A1 YEAST STRAIN HAVING IMPROVED CAPABILITY FOR FERMENTING XYLOSE IN THE PRESENCE OF ACETIC ACID 审中-公开
公开(公告)号：US20200208098A1
公开(公告)日：2020-07-02
申请号：US16793372
申请日：2020-02-18
申请人： LESAFFRE et COMPAGNIE
发明人： Jean-Michel Bavouzet , Thomas Desfougeres , Georges Pignede , Jennifer Techel
IPC分类号： C12N1/18 , C12N1/36 , C12R1/865 , C12N1/16 , C12P7/10 , C12P7/06
摘要： The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.

5. 发明申请

US20180155674A1 YEAST STRAIN HAVING IMPROVED CAPABILITY FOR FERMENTING XYLOSE IN THE PRESENCE OF ACETIC ACID 审中-公开
公开(公告)号：US20180155674A1
公开(公告)日：2018-06-07
申请号：US15568188
申请日：2016-04-27
申请人： LESAFFRE ET COMPAGNIE
发明人： Jean-Michel Bavouzet , Thomas Desfougeres , Georges Pignede , Jennifer Techel
IPC分类号： C12N1/18 , C12P7/06
CPC分类号： C12N1/18 , C12N1/16 , C12N1/36 , C12P7/06 , C12P7/10 , C12R1/865 , Y02E50/16 , Y02E50/17
摘要： The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.

6. 发明申请

US20160348064A1 PENTOSE-FERMENTING STRAIN WITH OPTIMIZED PROPAGATION 审中-公开
标题翻译：具有优化传播的赋形菌株
公开(公告)号：US20160348064A1
公开(公告)日：2016-12-01
申请号：US15116756
申请日：2015-02-16
申请人： LESAFFRE ET COMPAGNIE
发明人： Thomas Desfougeres , Georges Pignede , Jennifer Techel
IPC分类号： C12N1/36 , C12N9/92 , C12N9/12 , C12P7/10 , C12N15/81
CPC分类号： C12N1/36 , C12N9/1205 , C12N9/92 , C12N15/81 , C12P7/10 , C12R1/865 , C12Y207/01006 , C12Y207/01017 , C12Y503/01005 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to a method for obtaining a strain capable of efficiently propagating in a low nutritive potential medium, capable of metabolising pentoses and of resisting fermentation inhibitors, comprising the following steps; a) growth of a strain of recombinant yeast with a strain of wild yeast lacking any impairments, the recombinant yeast strain comprising at least one copy of an exogenous gene of xylose isomerase and at least one additional copy of a gene of D-xylulokinase included in the genome and linked to a single sexual characteristic of the strain, b) at least two cycles of genome shuffling by sporulation and or random hybridization, c) selection of the population obtained in step b) according to a suitability criterion of the strains to metabolize xylose, d) selection of the population obtained in step c) according to a suitability criterion of the strains to grow in a Pref type medium, a medium with low nutritive value. The invention also relates to a cell, a yeast or a strain of recombinant yeast obtained according to the method, and to the use of such a cell for the production of bioethanol.
摘要翻译：本发明涉及一种获得能够在低营养潜能培养基中有效繁殖的能够代谢戊糖和抵抗发酵抑制剂的菌株的方法，包括以下步骤： a）用缺乏任何损伤的野生酵母菌株生长重组酵母菌株，所述重组酵母菌株包含至少一个拷贝的木糖异构酶的外源基因和至少一个额外拷贝的D-木酮糖激酶基因，其包括在基因组并与菌株的单一性特征相关联，b）通过孢子形成和/或随机杂交进行基因组改组的至少两个循环，c）根据菌株代谢的适宜性标准选择步骤b）中获得的群体木糖，d）根据在Pref型培养基，营养价值低的培养基中生长菌株的适合性标准，选择步骤c）中获得的群体。本发明还涉及根据该方法获得的细胞，酵母或重组酵母菌株，以及这种细胞用于生产生物乙醇的用途。

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