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1. 发明授权

US10006020B2 Prokaryotic xylose isomerase for the construction of xylose-fermenting yeasts 有权
公开(公告)号：US10006020B2
公开(公告)日：2018-06-26
申请号：US14572137
申请日：2014-12-16
申请人： LESAFFRE ET COMPAGNIE
发明人： Dawid Brat , Eckhard Boles , Marco Keller , Beate Rother
IPC分类号： C12P7/56 , C12P7/54 , C12P7/46 , C12P7/02 , C12P7/18 , C12P7/20 , C12P7/16 , C12P7/06 , C12P5/00 , C12P5/02 , C12P1/02 , C12N9/90 , C12N1/00 , C12P21/00 , C12P19/34 , C12N15/00 , C07H21/04 , C07K1/00 , C12N9/92 , C12P7/10 , C12P19/24 , C12P19/02
CPC分类号： C12N9/92 , C12P7/06 , C12P7/065 , C12P7/10 , C12P7/16 , C12P19/02 , C12P19/24 , C12Y503/01005 , Y02E50/10 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol. The present invention also relates to methods for the production of bioethanol, and to methods for the production of further metabolization products, comprising the metabolization of media containing xylose.

2. 发明授权

US09951326B2 Enhancing microbial metabolism of C5 organic carbon 有权
公开(公告)号：US09951326B2
公开(公告)日：2018-04-24
申请号：US15208849
申请日：2016-07-13
申请人： MARA Renewables Corporation
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
IPC分类号： C12N1/11 , C12N9/92 , C12N9/12 , C07K14/40
CPC分类号： C12N9/92 , C07K14/40 , C07K2319/21 , C12N9/1205 , C12Y207/01017 , C12Y503/01005
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.

3. 发明授权

US09796991B2 Recombinant strain producing L-amino acids, constructing method therefor and method for producing L-amino acids 有权
公开(公告)号：US09796991B2
公开(公告)日：2017-10-24
申请号：US14891622
申请日：2015-02-04
申请人： INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES
发明人： Tingyi Wen , Xiuling Shang , Yun Zhang , Shuwen Liu , Yong Liang , Yu Zhang
IPC分类号： C12P13/04 , C12P13/24 , C12N9/04 , C12N9/92 , C12N15/77
CPC分类号： C12P13/24 , C12N9/0006 , C12N9/92 , C12N15/77 , C12Y101/01043 , C12Y101/01049 , C12Y503/01009
摘要： The present invention relates to recombinant bacteria producing L-amino acid, in which the recombinant bacteria has reduced expression of the glucose-6-phosphate isomerase gene pgi and improved expression of the glucose-6-phosphate dehydrogenase gene -opcA than the starting bacteria, where the starting bacterium is a bacterial strain that can accumulate target amino acid(s) and preferably, the amino acid is L-histidine.

4. 发明申请

US20170268012A1 PENTOSE SUGAR FERMENTING CELL 审中-公开
公开(公告)号：US20170268012A1
公开(公告)日：2017-09-21
申请号：US15612728
申请日：2017-06-02
申请人： DSM IP ASSETS B.V.
发明人： Paul KLAASSEN , Gijsberdina Pieternella Van Suylekom , Rene Marcel De Jong
IPC分类号： C12N15/81 , C12P5/02 , C12P7/10 , C12P7/56 , C12P7/54 , C12P7/48 , C12P7/46 , C12P7/42 , C12P35/00 , C12P7/20 , C12P7/18 , C12P7/16 , C12P7/06 , C12P7/40 , C12N9/92
CPC分类号： C12N15/81 , C12N9/92 , C12N15/815 , C12P5/026 , C12P7/06 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P35/00 , C12Y503/01005 , Y02E50/16 , Y02E50/17
摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

5. 发明授权

US09752171B2 Pentose fermentation by a recombinant microorganism 有权
公开(公告)号：US09752171B2
公开(公告)日：2017-09-05
申请号：US14871483
申请日：2015-09-30
申请人： Codexis, Inc.
发明人： Ezhilkani Subbian , Xiyun Zhang , Benjamin N. Mijts , Catherine M. Cho
IPC分类号： C12N1/00 , C12P19/24 , C12N9/90 , C12P7/04 , C12P7/10 , C12N15/81 , C12N9/92 , C12P19/02
CPC分类号： C12P19/24 , C12N9/90 , C12N9/92 , C12N15/81 , C12P7/04 , C12P7/10 , C12P19/02 , C12P2203/00 , C12Y503/01005 , Y02E50/16 , Y02E50/17
摘要： The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.

6. 发明申请

US20170114350A1 EXPRESSION CARTRIDGE FOR THE TRANSFORMATION OF EUKARYOTIC CELLS, METHOD FOR TRANSFORMING EUKARYOTIC CELLS, GENETICALLY MODIFIED ORGANISM, METHOD FOR PRODUCING BIOFUELS AND/OR BIOCHEMICALS, AND THUS PRODUCED BIOFUEL AND/OR BIOCHEMICAL 审中-公开
公开(公告)号：US20170114350A1
公开(公告)日：2017-04-27
申请号：US15315081
申请日：2015-06-12
申请人： Bioclere Agroindustrial Ltda.
发明人： Leandro Vieira dos Santos , Gonçalo Amarante Guimarães Pereira
IPC分类号： C12N15/81 , C12N9/92 , C12P7/06
CPC分类号： C12N15/81 , C12N9/92 , C12N15/52 , C12P7/06 , C12Y503/01005 , Y02E50/17
摘要： The present invention describes the expression cassette for transforming eukaryotic cell which comprises the peptide encoding non-natural sequence of nucleotides with xylose isomerase feature (SEQ ID NO: 1), optionally also comprising other genes of pentose phosphate route. Additionally, it is described the microorganism filed under the number DSM28739, which, in addition to the above-mentioned modifications, also present genetic modifications from adaptive evolution. The described microorganism shows efficient consumption of xylose and conversion of ethanol when compared to its correspondent without said genetic modifications and mutations from evolution. It is also described the process for producing biofuels e biochemicals, preferably ethanol, mainly from the lignocellulosic portion of the vegetal biomass. Biofuels, preferably ethanol, and biochemicals produced by the process of the invention are also described.

7. 发明申请

US20170015988A1 ENHANCING MICROBIAL METABOLISM OF C5 ORGANIC CARBON 有权
标题翻译：增加C5有机碳的微生物代谢
公开(公告)号：US20170015988A1
公开(公告)日：2017-01-19
申请号：US15208849
申请日：2016-07-13
申请人： MARA Renewables Corporation
发明人： Alexandra Merkx-Jacques , David Woodhall , Mark Scaife , Roberto E. Armenta , Denise Muise , Holly Rasmussen , Jeremy Benjamin
IPC分类号： C12N9/92 , C07K14/40 , C12N9/12
CPC分类号： C12N9/92 , C07K14/40 , C07K2319/21 , C12N9/1205 , C12Y207/01017 , C12Y503/01005
摘要： Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.
摘要翻译：本文提供了具有两个或多个拷贝的编码木糖异构酶的核酸序列的重组微生物，其中编码木糖异构酶的核酸是外源核酸。任选地，重组微生物包括编码木酮糖激酶和/或编码木糖转运蛋白的至少一个核酸序列的至少一个核酸序列。所提供的重组微生物能够在作为碳源的木糖上生长。

8. 发明授权

US09376695B2 L-amino acid producing bacterium 有权
标题翻译： L-氨基酸生产菌
公开(公告)号：US09376695B2
公开(公告)日：2016-06-28
申请号：US12238704
申请日：2008-09-26
申请人： Rustem Saidovich Shakulov , Elena Vitalievna Klyachko , Vera Georgievna Doroshenko , Larisa Gotlibovna Airikh
发明人： Rustem Saidovich Shakulov , Elena Vitalievna Klyachko , Vera Georgievna Doroshenko , Larisa Gotlibovna Airikh
IPC分类号： C12P13/22 , C12P13/20 , C12P13/24 , C12P21/02 , C12N9/02 , C12N9/90 , C12N9/92
CPC分类号： C12P21/02 , C12N9/001 , C12N9/90 , C12N9/92 , C12P13/20 , C12P13/222 , C12P13/24 , C12Y103/01013 , C12Y503/01009 , C12Y504/99005 , Y02P20/52
摘要： A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase.
摘要翻译：通过使用肠杆菌科的细菌进行发酵，描述了L-氨基酸的制造方法，例如L-苯丙氨酸和L-组氨酸，其中，通过将能够转录的DNA片段连接到编码 SEQ ID NO：2所示的肽，或其变体，特别是ssrA基因的一部分，对编码细菌酶的基因的3'末端，其影响L-氨基酸生物合成，例如绒毛膜变位酶 / prephenate脱氢酶或磷酸葡萄糖异构酶。

9. 发明授权

US09365857B2 Xylose isomerase and xylitol dehydrogenase combination for xylose fermentation to ethanol and B. fragilis xylose isomerase 有权
标题翻译：木糖异构酶和木糖醇脱氢酶组合用于木糖发酵成乙醇和脆弱木糖木糖异构酶
公开(公告)号：US09365857B2
公开(公告)日：2016-06-14
申请号：US13996305
申请日：2011-12-09
申请人： Sean Jordan , Beth Fatland-Bloom , Lhing-Yew Li
发明人： Sean Jordan , Beth Fatland-Bloom , Lhing-Yew Li
IPC分类号： C12P7/06 , C12P7/10 , C12N9/90 , C12N9/04 , C12N1/19 , C12N15/81 , C12N9/92
CPC分类号： C12N15/81 , C12N9/0006 , C12N9/92 , C12P7/10 , C12Y101/01009 , C12Y503/01005 , Y02E50/16 , Y02E50/17 , Y02P20/52
摘要： Disclosed herein is a newly discovered problem and solution for engineering S. cerevisiae to ferment xylose to make ethanol utilizing xylose isomerase to convert xylose to xylulose for entry, via xylulokinase, into the pentose phosphate pathway. The problem is that when grown on a media containing xylose xylitol tends to accumulate in the cell despite the absence of xylose reductase activity in S. cerevisiae. Xylitol inhibits the activity of xylose isomerases. One solution described is to simultaneously express an exogenous xylitol dehydrogenase along with the exogenous xylose isomerase while optionally also overexpressing xylulokinase in the absence of expression of a xylose reductase. Another solution is a xylose isomerase from Bacteroides fragilis which is less inhibited by xylitol than other xylose isomerases, exemplified by E. coli xylose isomerase. Expression of the Bacteroides fragilis xylose isomerase may be used alone, or in combination with expression of a xylitol dehydrogenase and optionally over expression of xylulokinase to improve ethanol production from xylose.
摘要翻译：本文公开了一种新发现的问题和解决方案，用于工程酿酒酵母发酵木糖以制备乙醇，利用木糖异构酶将木糖转化成木酮糖，通过木酮糖激酶进入戊糖磷酸途径。问题是，当在含有木糖木糖醇的培养基上生长时，尽管在酿酒酵母中不存在木糖还原酶，但仍倾向于在细胞中积累。木糖醇抑制木糖异构酶的活性。所描述的一种溶液是同时表达外源性木糖异构酶外源木糖醇脱氢酶，而在不存在木糖还原酶表达的情况下，任选也可以过表达木酮糖激酶。另一种溶液是来自脆弱拟杆菌的木糖异构酶，木糖异构酶比其它木糖异构酶抑制较少，木糖异构酶例如是大肠杆菌木糖异构酶。脆弱拟杆菌木糖异构酶的表达可以单独使用，或与木糖醇脱氢酶的表达和任选地过度表达木酮糖激酶一起使用以改善从木糖产生乙醇。

10. 发明申请

US20160130590A1 BACTERIAL XYLOSE ISOMERASES ACTIVE IN YEAST CELLS 审中-公开
标题翻译：细菌中的微生物异黄酮活性在YEAST细胞中
公开(公告)号：US20160130590A1
公开(公告)日：2016-05-12
申请号：US14881563
申请日：2015-10-13
申请人： E I Du Pont De Nemours And Company
发明人： ZHONGQIANG CHEN , Kristen J Kelly , Rick W Ye
IPC分类号： C12N15/81 , C12N9/92
CPC分类号： C12N15/81 , C12N9/90 , C12N9/92 , C12P7/10 , C12P7/16 , C12P7/18 , C12Y503/01005 , Y02E50/10 , Y02E50/16
摘要： Specific polypeptides were identified as bacterial xylose isomerases that are able to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate.
摘要翻译：特异性多肽被鉴定为能够在酵母细胞中提供木糖异构酶活性的细菌木糖异构酶。木糖异构酶活性可以完成木糖利用途径，使得酵母可以在发酵中使用木糖，例如生物质水解产物中的木糖。

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