专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US10806801B2 Pharmaceutical composition and methods for using the same 有权
公开(公告)号：US10806801B2
公开(公告)日：2020-10-20
申请号：US15997715
申请日：2018-06-05
申请人： NATIONAL CHENG KUNG UNIVERSITY , NATIONAL CHENG KUNG UNIVERSITY HOSPITAL , NATIONAL TAIWAN NORMAL UNIVERSITY
发明人： Wu-Chou Su , Hai-Wen Chen , Tsung-Lin Tsai , Dar-Bin Shieh , Chia-Chun Chen
IPC分类号： A61K47/68 , A61K47/69 , A61K31/137 , A61K49/18 , A61K49/04 , A61K9/51 , A61K41/00 , A61K47/58 , A61K47/59 , A61K47/54 , A61K47/60 , A61B5/055 , A61B6/03 , A61K31/661 , A61K31/662 , A61N5/10 , A61K9/00
摘要： A pharmaceutical composition includes a plurality of metal nanoparticles and at least one therapeutic agent. Each of the metal nanoparticles includes a core and a stabilizing agent coated on a surface of the core. The at least one therapeutic agent is attached to the stabilizing agent of the metal nanoparticles. Each of the therapeutic agent is an amphiphilic compound and has at least one hydrophobic chain interacting with the stabilizing agent. The pharmaceutical composition may further include a polymer shell encapsulating the metal nanoparticles and the therapeutic agent for enabling controlled release of the therapeutic agent. The pharmaceutical compositions are bifunctional and may be used for diagnosing and treating cancer. Methods for using the pharmaceutical compositions in conjunction with radiation therapy to diagnose and treat cancer are also provided.

2. 发明授权

US11786587B2 Composition of subunit dengue vaccine 有权
公开(公告)号：US11786587B2
公开(公告)日：2023-10-17
申请号：US16906134
申请日：2020-06-19
申请人： National Cheng Kung University
发明人： Yee-Shin Lin , Trai-Ming Yeh , Yung-Chun Chuang , Chia-Yi Yu , Hsin-Wei Chen , Shu-Wen Wan , Shu-Ying Wang , Tzong-Shiann Ho , Dar-Bin Shieh
IPC分类号： A61K39/12 , C12N7/00 , C07K14/005
CPC分类号： A61K39/12 , C07K14/005 , C12N7/00 , C07K2319/00 , C12N2770/24131
摘要： The present invention relates to a composition of subunit dengue vaccine comprising a fusion protein of conjugating or connecting delta C nonstructural protein 1 (NS1ΔC or truncated NS1ΔC) to at least one polypeptides of NS3c (or truncated NS3c) and/or consensus envelope protein domain III (cEDIII), thereby enhancing better protection against DENV challenge and alleviating associated pathological effects.

3. 发明授权

US09551665B2 Method for detecting mitochondria gene alterations 有权
标题翻译：检测线粒体基因改变的方法
公开(公告)号：US09551665B2
公开(公告)日：2017-01-24
申请号：US13632510
申请日：2012-10-01
申请人： NATIONAL CHENG KUNG UNIVERSITY
发明人： Dar-Bin Shieh , Gwo-Bin Lee , Chen-Min Chang , Li-Fang Chiu
IPC分类号： C12Q1/68 , G01N21/64
CPC分类号： G01N21/6486 , C12Q1/6851 , C12Q2561/113 , C12Q2565/629
摘要： The present invention relates to a method for detecting mitochondria alterations, which comprises the following steps: (A) providing a separation element and a sample; (B) mixing the separation element and the sample, wherein a detecting sample is obtained through the binding of a DNA fragment on the separation element to mitochondrial DNA in the sample; (C) dividing the detecting sample into a comparison group and a detection group; (D) adding an amplification solution into the comparison group and the detection group respectively to begin a DNA amplified reaction, and further adding a restriction enzyme into the detection group, wherein the amplification solution comprises a labeling reagent and a primer pair; and (E) detecting amounts of the labeling reagent in the comparison group and the detection group respectively after the DNA amplified reaction.
摘要翻译：本发明涉及一种检测线粒体改变的方法，包括以下步骤：（A）提供分离元件和样品; （B）混合分离元件和样品，其中通过将分离元件上的DNA片段与样品中的线粒体DNA结合获得检测样品; （C）将检测样本分成比较组和检测组; （D）分别向比较组和检测组中加入扩增溶液开始DNA扩增反应，并进一步向检测基中加入限制酶，其中扩增溶液包含标记试剂和引物对; 和（E）分别检测DNA扩增反应后的比较组和检测组中的标记试剂的量。

4. 发明授权

US10913069B2 Method and device for polymerase chain reaction 有权
公开(公告)号：US10913069B2
公开(公告)日：2021-02-09
申请号：US16244133
申请日：2019-01-10
申请人： NATIONAL CHENG KUNG UNIVERSITY
发明人： Dar-Bin Shieh , Chih-Chia Huang , Chen-Min Chang , Tsung-Ju Li , Po-Yang Chang , Ming-Chi Hsieh
IPC分类号： B01L7/00 , C01G49/08 , B01L9/06
摘要： An apparatus for amplifying a nucleic acid sequence in a polymerase chain reaction (PCR) is provided. The apparatus comprises a microprocessor configured to communicatively couple with a user input device and receive instructions from the user input device and implement the received instructions; an electromagnetic radiation (EMR) generator configured to generated EMR at frequencies ranging from 300 THz to 400 THz and communicatively coupled with the microprocessor; a reaction vessel, communicatively coupled with the microprocessor, to house the nucleic acid sequence, necessary reagents to the PCR, and particles comprising a transition metal material, and to receive the EMR generated by the EMR generator; and a temperature sensor communicatively coupled with the reaction vessel and the microprocessor.

5. 发明申请

US20190307894A1 ENDOPLASMIC RETICULUM-TARGETING NANOVEHICLES AND METHODS FOR USE THEREOF 审中-公开
公开(公告)号：US20190307894A1
公开(公告)日：2019-10-10
申请号：US16263618
申请日：2019-01-31
申请人： National Cheng Kung University
发明人： Chi-Chang Shieh , Chia-Liang Yen , Dar-Bin Shieh , Si-Tse Jiang
IPC分类号： A61K47/69 , A61P37/00 , A61K36/9066 , A61K36/23 , A61K36/06 , A61K36/9062 , A61K36/22 , A61K47/59 , A61K9/00 , A01K67/027
摘要： A method for treating a disease caused by protein retention in the endoplasmic reticulum (ER) with a sarcoplasmic/endoplasmic reticulum calcium ATPase pump inhibitor encapsulated in a polymer nanoparticle. The polymer nanoparticle is surface-modified such that it is targeted to the ER. The inhibitor reduces protein retention in the ER and the encapsulation lowers side effects of the inhibitor, e.g., cytotoxicity, as compared to administering the inhibitor without encapsulation. Also disclosed is a pharmaceutical composition that can be used for carrying out the method. Further provided is a transgenic mouse carrying in its genome a heterologous nucleic acid that encodes an H338Y mutant gp91phox protein. The transgenic mouse can serve as a model for human chronic granulomatous disease.

6. 发明授权

US10213785B2 Method and device for polymerase chain reaction 有权
公开(公告)号：US10213785B2
公开(公告)日：2019-02-26
申请号：US14840833
申请日：2015-08-31
申请人： NATIONAL CHENG KUNG UNIVERSITY
发明人： Dar-Bin Shieh , Chih-Chia Huang , Chen-Min Chang , Tsung-Ju Li , Po-Yang Chang , Ming-Chi Hsieh
IPC分类号： C12Q1/68 , C12P19/34 , B01L7/00 , C01G49/08 , B01L9/06
摘要： Method and apparatus for amplifying a target nucleic acid sequence of a reaction mixture in a Polymerase Chain Reaction (PCR). The method includes contacting the reaction mixture with EMR frequency absorbing particles formed from a material having a transition metal, transition metal oxide or a transition metal hydroxide, or a nitride, a phosphide or an arsenide of a Group III metal doped with the transition metal or a transition metal oxide, or silicon dioxide doped with the transition metal, transition metal oxide, or transition metal hydroxide; and irradiating the EMR absorbing particles with EMR having a frequency of about 200 kHz to 500 THz to amplify the target nucleic acid sequence, wherein the Group III metal is any one of Al, Ga, and In, and the transition metal is any one of Mn, Fe, Co and Cu.

7. 发明授权

US09351941B2 Radiofrequency-induced synchronization of in situ hyperthermia and chemotherapy via magnetic-nanoconjugates 有权
标题翻译：射频诱导的原位高温同步化疗通过磁纳米结合物
公开(公告)号：US09351941B2
公开(公告)日：2016-05-31
申请号：US13905177
申请日：2013-05-30
申请人： National Cheng Kung University
发明人： Dar-Bin Shieh , Chen-Sheng Yeh , Tsung-Ju Li , Chih-Chia Huang
IPC分类号： A61K9/16 , A61K9/50 , A61K9/51 , A61K47/48
CPC分类号： A61K9/5169 , A61K47/48569 , A61K47/48861 , A61K47/6851 , A61K47/6923
摘要： The present invention relates to a magnetic nanoparticle for tumor therapy, comprising: a magnetic core; a shell encapsulating a surface of the magnetic core, wherein the shell is made of a polymer with carboxylic groups; a poly-nucleotide chain connected to a surface of the shell; an anti-tumor drug connected to the poly-nucleotide chain, wherein the anti-tumor drug comprises at least one functional group, and each of the functional group is independently a pyrimidine group or a purine group; and an antibody connected to the shell, wherein the antibody identifies a target tumor. In addition, the present invention further provides a method for manufacturing the magnetic nanoparticles for tumor therapy and a pharmaceutical composition containing the magnetic nanoparticles. Accordingly, the magnetic nanoparticle for tumor therapy of the present invention can achieve effective treatment of tumor by synergistic effects between hyperthermia and targeted chemotherapy.
摘要翻译：本发明涉及一种用于肿瘤治疗的磁性纳米颗粒，包括：磁芯; 壳体，其包封所述磁芯的表面，其中所述壳由具有羧基的聚合物制成; 连接到壳的表面的多核苷酸链; 与多核苷酸链连接的抗肿瘤药物，其中所述抗肿瘤药物包含至少一个官能团，所述官能团各自独立地为嘧啶基或嘌呤基; 和与壳连接的抗体，其中抗体识别靶肿瘤。此外，本发明还提供了一种用于制造用于肿瘤治疗的磁性纳米颗粒的方法和含有磁性纳米颗粒的药物组合物。因此，本发明的肿瘤治疗用磁性纳米粒子可以通过热疗与靶向化疗之间的协同效应来实现肿瘤的有效治疗。

8. 发明授权

US11214840B2 Methods for treating periodontal diseases 有权
公开(公告)号：US11214840B2
公开(公告)日：2022-01-04
申请号：US16594072
申请日：2019-10-07
申请人： NATIONAL CHENG KUNG UNIVERSITY
发明人： Ping-Ching Wu , Dar-Bin Shieh , Fong-Yu Cheng , Yuh-Ling Chen
IPC分类号： C12Q1/689 , A61N5/06 , A61C1/08 , A61C5/30
摘要： Provided herein are aptamers that bind specifically to the surface proteins of periodontal pathogens. Also provided herein are methods for detecting and treating periodontal disease in a subject. The method comprises detecting bacteria associated with periodontal disease by use of the present aptamers to analyze a sample taken from a gum pocket of a tooth of the subject; and treating the periodontal disease of the subject by administering to the gum pocket of the tooth a bactericide or an anti-microbial phototherapy to eradicate the bacteria associated with periodontal disease.

9. 发明授权

US10912843B2 Endoplasmic reticulum-targeting nanovehicles and methods for use thereof 有权
公开(公告)号：US10912843B2
公开(公告)日：2021-02-09
申请号：US16263618
申请日：2019-01-31
申请人： National Cheng Kung University
发明人： Chi-Chang Shieh , Chia-Liang Yen , Dar-Bin Shieh , Si-Tse Jiang
IPC分类号： A61K47/69 , A61K36/9066 , A61K47/59 , A61P37/00 , A01K67/027 , A61K9/00 , A61K36/06 , A61K36/22 , A61K36/23 , A61K36/9062 , A61K31/05 , A61K31/365 , A61K31/55 , A61K31/407 , A61K45/06 , A61K31/12 , A61K31/58 , A61P43/00 , A61K31/7048
摘要： A method for treating a disease caused by protein retention in the endoplasmic reticulum (ER) with a sarcoplasmic/endoplasmic reticulum calcium ATPase pump inhibitor encapsulated in a polymer nanoparticle. The polymer nanoparticle is surface-modified such that it is targeted to the ER. The inhibitor reduces protein retention in the ER and the encapsulation lowers side effects of the inhibitor, e.g., cytotoxicity, as compared to administering the inhibitor without encapsulation. Also disclosed is a pharmaceutical composition that can be used for carrying out the method. Further provided is a transgenic mouse carrying in its genome a heterologous nucleic acid that encodes an H338Y mutant gp91phox protein. The transgenic mouse can serve as a model for human chronic granulomatous disease.

10. 发明授权

US10487331B1 Aptamers of surface proteins of periodontal pathogens and uses thereof 有权
公开(公告)号：US10487331B1
公开(公告)日：2019-11-26
申请号：US16205236
申请日：2018-11-30
申请人： NATIONAL CHENG KUNG UNIVERSITY
发明人： Ping-Ching Wu , Dar-Bin Shieh , Fong-Yu Cheng , Yuh-Ling Chen
IPC分类号： C12N15/115 , A61B5/00 , C12Q1/689 , C12Q1/6825 , C12Q1/68
CPC分类号： C12N15/115 , A61B5/00 , C12Q1/689 , C12Q1/6825
摘要： Disclosed herein are aptamers that bind specifically to the surface proteins of periodontal pathogens. Disclosed also herein are kits and methods for detecting bacteria associated with periodontal disease by using the present aptamers to analyze a sample taken from the oral cavity of a patient.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式