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1. 发明授权

US09968932B2 Microchannel chip for microparticle separation, microparticle separation method and system for microparticle separation using chip 有权
公开(公告)号：US09968932B2
公开(公告)日：2018-05-15
申请号：US14436055
申请日：2013-10-15
申请人： NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
发明人： Fumihito Arai , Taisuke Masuda , Miyako Niimi , Haruki Douke , Hayao Nakanishi , Seiji Ito
IPC分类号： B01L3/00 , G01N15/14 , G01N1/04 , G01N1/28 , G01N35/08 , G01N15/10
CPC分类号： B01L3/502753 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/086 , G01N1/04 , G01N1/28 , G01N15/14 , G01N15/1434 , G01N33/491 , G01N35/08 , G01N2015/1006 , G01N2015/149
摘要： Provided is a microchannel chip, for microparticle separation, which can continuously separate microparticles, from a solution in which microparticles of different particle diameter are mixed, without needing to use an antibody or similar, and without causing clogging. Also provided are a microparticle separation method and a system for microparticle separation using the chip. Microparticles can be captured by using the microchannel chip for microparticle separation, the microchannel chip being characterised by being provided with a plurality of main channels and capture portions that are wider than the main channels and at least one of which is provided to each main channel.

2. 发明授权

US11225639B2 Cell sorting device 有权
公开(公告)号：US11225639B2
公开(公告)日：2022-01-18
申请号：US16085412
申请日：2017-03-06
申请人： National University Corporation Nagoya University
发明人： Shinya Sakuma , Takeshi Hayakawa , Fumihito Arai
IPC分类号： C12M1/00
摘要： A cell sorting device is characterized by including: a main flow channel (14) through which a liquid containing a row of cells including a target cell flows, and in which a cell separation area for separating the target cell from the row of cells is provided at a halfway position in a flow direction, the main flow channel being formed in a substrate (12); a selection flow channel (16) into which the target cell is pushed out from the main flow channel, the selection flow channel being formed in the substrate so as to branch from the main flow channel downstream of the cell separation area; a sub flow channel (18) through which a separating liquid flows, the separating liquid being for separating the target cell from the row of cells and pushing out the target cell into the selection flow channel, the sub flow channel being formed in the substrate so as to intersect with the main flow channel in the cell separation area; a pair of liquid flow generating members (22) for generating a liquid flow of the separating liquid in the sub flow channel, the liquid flow generating members being provided at one end and the other end of the sub flow channel; and a drive control member (23) for actuating the pair of liquid flow generating members, wherein the drive control member actuates the pair of liquid flow generating members in conjunction with each other so that the separating liquid forms a liquid flow in a first direction from the one end to the other end of the sub flow channel and a liquid flow in a second direction opposite to the first direction.

3. 发明授权

US10669525B2 Method for producing three-dimensional cell aggregates 审中-公开
公开(公告)号：US10669525B2
公开(公告)日：2020-06-02
申请号：US15515013
申请日：2015-09-29
申请人： PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY , OSAKA UNIVERSITY , NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
发明人： Utako Yokoyama , Yoshihiro Ishikawa , Makoto Kaneko , Shinya Sakuma , Fumihito Arai
IPC分类号： C12M1/24 , C12M1/00 , C12M3/00 , C12N5/00 , C12N5/077 , C12M1/12 , C12M1/22 , C12M1/34
摘要： Means which enables preparation of a thick cell aggregate by a simple process without an operation of detaching and stacking of cells is disclosed. The method for preparing a three-dimensional cell aggregate by the present invention comprises: a cell encasing step of placing a cell suspension in a cell container; and a pressure application step of applying pressure to cells in the container. The cell encasing step and the pressure application step may be carried out a plurality of times. By the present invention, a thick, robust cell aggregate can be obtained by a simple operation of applying pressure to a cell suspension or a medium containing cells. Since the method does not require an operation of stacking a plurality of cell sheets, the cells are hardly damaged, and the conditions of the cells can be favorably maintained, so that the cells can be advantageously used as a tissue piece for transplantation.

4. 发明授权

US10317397B2 Microparticle separation chip, and microparticle separation system and microparticle separation method which employ said microparticle separation chip 有权
公开(公告)号：US10317397B2
公开(公告)日：2019-06-11
申请号：US15303040
申请日：2015-04-09
申请人： National University Corporation Nagoya University
发明人： Fumihito Arai , Taisuke Masuda , Woneui Song
IPC分类号： G01N33/543 , G01N33/574 , B01L3/00 , G01N33/50
摘要： Provided is a microparticle separation system capable of continuously separating microparticles from a solution in a short period of time in which microparticles having different particle diameters are mixed, without the need to use antibodies or the like. The microparticle separation system comprises a microparticle separation chip; a thin plate for a sample liquid; a thin plate for a sheath liquid; and suctioning means and/or a suctioning device for suctioning the sheath liquid; and the microparticle separation chip comprises a single capture site for capturing to-be-captured microparticles being formed using the at least three pillars having one end provided on the substrate and the other end open upward.

5. 发明申请

US20190071630A1 CELL SORTING DEVICE 审中-公开
公开(公告)号：US20190071630A1
公开(公告)日：2019-03-07
申请号：US16085412
申请日：2017-03-06
申请人： National University Corporation Nagoya University
发明人： Shinya Sakuma , Takeshi Hayakawa , Fumihito Arai
IPC分类号： C12M1/00
摘要： A cell sorting device is characterized by including: a main flow channel (14) through which a liquid containing a row of cells including a target cell flows, and in which a cell separation area for separating the target cell from the row of cells is provided at a halfway position in a flow direction, the main flow channel being formed in a substrate (12); a selection flow channel (16) into which the target cell is pushed out from the main flow channel, the selection flow channel being formed in the substrate so as to branch from the main flow channel downstream of the cell separation area; a sub flow channel (18) through which a separating liquid flows, the separating liquid being for separating the target cell from the row of cells and pushing out the target cell into the selection flow channel, the sub flow channel being formed in the substrate so as to intersect with the main flow channel in the cell separation area; a pair of liquid flow generating members (22) for generating a liquid flow of the separating liquid in the sub flow channel, the liquid flow generating members being provided at one end and the other end of the sub flow channel; and a drive control member (23) for actuating the pair of liquid flow generating members, wherein the drive control member actuates the pair of liquid flow generating members in conjunction with each other so that the separating liquid forms a liquid flow in a first direction from the one end to the other end of the sub flow channel and a liquid flow in a second direction opposite to the first direction.

6. 发明申请

US20150246353A1 MICROCHANNEL CHIP FOR MICROPARTICLE SEPARATION, MICROPARTICLE SEPARATION METHOD AND SYSTEM FOR MICROPARTICLE SEPARATION USING CHIP 有权
标题翻译：用于微波分离的微通道芯片，微波分离方法和使用芯片的微波分离系统
公开(公告)号：US20150246353A1
公开(公告)日：2015-09-03
申请号：US14436055
申请日：2013-10-15
申请人： NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY , AICHI PREFECTURE
发明人： Fumihito Arai , Taisuke Masuda , Miyako Niimi , Haruki Douke , Hayao Nakanishi , Seiji Ito
IPC分类号： B01L3/00
CPC分类号： B01L3/502753 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/086 , G01N1/04 , G01N1/28 , G01N15/14 , G01N15/1434 , G01N33/491 , G01N35/08 , G01N2015/1006 , G01N2015/149
摘要： Provided is a microchannel chip, for microparticle separation, which can continuously separate microparticles, from a solution in which microparticles of different particle diameter are mixed, without needing to use an antibody or similar, and without causing clogging. Also provided are a microparticle separation method and a system for microparticle separation using the chip. Microparticles can be captured by using the microchannel chip for microparticle separation, the microchannel chip being characterised by being provided with a plurality of main channels and capture portions that are wider than the main channels and at least one of which is provided to each main channel.
摘要翻译：本发明提供一种用于微粒分离的微通道芯片，其可以将微粒从混合不同粒径的微粒的溶液连续分离，而不需要使用抗体或类似物，并且不引起堵塞。还提供了微粒分离方法和使用该芯片的微粒分离系统。可以通过使用微通道芯片捕获微粒用于微粒分离，微通道芯片的特征在于具有多个主通道和比主通道宽的捕获部分，并且其中至少一个设置在每个主通道上。

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