专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US11591649B2 Methods for amplification of cell-free DNA using ligated adaptors and universal and inner target-specific primers for multiplexed nested PCR 有权
公开(公告)号：US11591649B2
公开(公告)日：2023-02-28
申请号：US16796748
申请日：2020-02-20
申请人： Natera, Inc.
发明人： Matthew Rabinowitz , Matthew Hill , Bernhard Zimmermann , Johan Baner , George Gemelos , Milena Banjevic , Allison Ryan , Styrmir Sigurjonsson , Zachary Demko
IPC分类号： C12Q1/6869 , G16B10/00 , C12Q1/6876 , C12Q1/6844
摘要： Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

2. 发明授权

US11408031B2 Methods for non-invasive prenatal paternity testing 有权
公开(公告)号：US11408031B2
公开(公告)日：2022-08-09
申请号：US16012667
申请日：2018-06-19
申请人： Natera, Inc.
发明人： Allison Ryan , Styrmir Sigurjonsson , Milena Banjevic , George Gemelos , Matthew Hill , Johan Baner , Matthew Rabinowitz , Zachary Demko , Bernhard Zimmerman
IPC分类号： C12Q1/68 , C12Q1/6869 , C12Q1/6876 , C12Q1/6844 , G16B10/00
摘要： Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

3. 发明授权

US09163282B2 Methods for non-invasive prenatal ploidy calling 有权
标题翻译：非侵入性产前倍性调用方法
公开(公告)号：US09163282B2
公开(公告)日：2015-10-20
申请号：US13791397
申请日：2013-03-08
申请人： Natera, Inc.
发明人： Matthew Rabinowitz , George Gemelos , Milena Banjevic , Allison Ryan , Zachary Demko , Matthew Hill , Bernhard Zimmermann , Johan Baner
IPC分类号： G01N33/48 , G01N31/00 , G06G7/48 , G06G7/58 , C12Q1/68 , G06F19/18 , G06F19/24
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6827 , C12Q1/6862 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , G06F19/18 , G06F19/24 , C12Q2537/161 , C12Q2537/165
摘要： The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.
摘要翻译：本公开提供了从包括来自胎儿母体和胎儿的DNA的DNA的混合样品测量的基因型数据以及任选地来自母体的基因型数据以及任选地从胎儿的基因型数据确定胎儿胎儿的染色体的倍性状态的方法父亲。通过使用联合分布模型来确定给定父母基因型数据的不同可能胎儿倍性状态的多个预期等位基因分布，并将预期等位基因分布与混合样品中测量的等位基因分布的模式进行比较来确定倍性状态，并选择其预期等位基因分布模式与观察到的等位基因分布模式最接近的倍性状态。 DNA的混合样品可以以使等位基因偏倚最小化的方式优先富集在多个多态性位点，例如使用大规模复用的靶向PCR。

4. 发明申请

US20130196862A1 Informatics Enhanced Analysis of Fetal Samples Subject to Maternal Contamination 审中-公开
标题翻译：母亲污染胎儿样本的信息学增强分析
公开(公告)号：US20130196862A1
公开(公告)日：2013-08-01
申请号：US13780022
申请日：2013-02-28
申请人： Natera, Inc.
发明人： Matthew Rabinowitz , Johan Baner , George Gemelos , Mathew M. Hill , Stymir Sigurjonsson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6881 , C12Q2600/156 , G16B20/00
摘要： The invention provides methods for chromosome copy number calling on genetic samples, such as fetal samples subject to contamination from maternal DNA. The present disclosure provides methods for determining the ploidy status of a chromosome in a fetus (such as a gestating fetus or a POC sample) from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. In some embodiments, the ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.
摘要翻译：本发明提供了染色体拷贝数调用基因样品的方法，例如受母体DNA污染的胎儿样品。本公开提供了从包含来自胎儿母体和胎儿的DNA的DNA的混合样品测量的基因型数据中确定胎儿（例如胎儿胎儿或POC样品）中染色体的倍性状态的方法，以及来自母亲和父亲的基因型数据。在一些实施方案中，通过使用联合分布模型来确定给定父母基因型数据的不同可能的胎儿倍性状态的多个预期等位基因分布，并将预期的等位基因分布与所测量的等位基因分布的模式进行比较来确定倍性状态混合样品，并选择其预期等位基因分布模式与观察到的等位基因分布模式最为匹配的倍性状态。 DNA的混合样品可以以使等位基因偏倚最小化的方式优先富集在多个多态性位点，例如使用大规模复用的靶向PCR。

5. 发明授权

US11345962B2 Methods for amplification of cell-free DNA using ligated adaptors and universal and inner target-specific primers for multiplexed nested PCR 有权
公开(公告)号：US11345962B2
公开(公告)日：2022-05-31
申请号：US16796748
申请日：2020-02-20
申请人： Natera, Inc.
发明人： Matthew Rabinowitz , Matthew Hill , Bernhard Zimmermann , Johan Baner , George Gemelos , Milena Banjevic , Allison Ryan , Styrmir Sigurjonsson , Zachary Demko
IPC分类号： C12Q1/6869 , G16B10/00 , C12Q1/6876 , C12Q1/6844
摘要： Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

6. 发明授权

US11111545B2 Methods for simultaneous amplification of target loci 有权
公开(公告)号：US11111545B2
公开(公告)日：2021-09-07
申请号：US17061877
申请日：2020-10-02
申请人： Natera, Inc.
发明人： Joshua Babiarz , Tudor Pompiliu Constantin , Lane A. Eubank , George Gemelos , Matthew Micah Hill , Huseyin Eser Kirkizlar , Matthew Rabinowitz , Onur Sakarya , Styrmir Sigurjonsson , Bernhard Zimmermann , Johan Baner , Allison Ryan , Milena Banjevic , Zachary Demko
IPC分类号： C12Q1/68 , C12Q1/6883 , C12Q1/6811 , C12Q1/6848 , C12Q1/6851 , C12Q1/6855 , C12Q1/6874 , C12Q1/6809
摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

7. 发明申请

US20190256908A1 METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING 审中-公开
公开(公告)号：US20190256908A1
公开(公告)日：2019-08-22
申请号：US16399991
申请日：2019-04-30
申请人： Natera, Inc.
发明人： Matthew Rabinowitz , George Gemelos , Milena Banjevic , Allison Ryan , Zachary Demko , Matthew Hill , Bernhard Zimmermann , Johan Baner
IPC分类号： C12Q1/6869 , C12Q1/6862 , C12Q1/6806 , G16B20/00 , C12Q1/6827 , C12Q1/6874 , C12Q1/6883
摘要： The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.

8. 发明申请

US20150072872A1 METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING 审中-公开
标题翻译：非侵入式预防性呼吸方法
公开(公告)号：US20150072872A1
公开(公告)日：2015-03-12
申请号：US14546321
申请日：2014-11-18
申请人： Natera, Inc.
发明人： Matthew Rabinowitz , George Gemelos , Milena Banjevic , Allison Ryan , Zachary Demko , Matthew Hill , Bernhard Zimmermann , Johan Baner
IPC分类号： C12Q1/68 , G06F19/00 , G06F19/18
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q1/6869 , C12Q2537/161 , C12Q2537/165 , C12Q2600/112 , C12Q2600/156 , C12Q2600/16 , C12Q2600/172 , G06F19/34 , G16B5/00 , G16B20/00 , G16B30/00 , G16H50/30
摘要： The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a sample of DNA from the mother of the fetus and from the fetus, and from genotypic data from the mother and optionally also from the father. The ploidy state is determined by using a joint distribution model to create a set of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. In an embodiment, the mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias.
摘要翻译：本公开提供了用于从胎儿母体和胎儿的DNA样品测量的基因型数据以及来自母亲的基因型数据以及任选地还可以从父亲的基因型数据确定胎儿胎儿中染色体的倍性状态的方法。通过使用联合分布模型来确定给定父母基因型数据的不同可能胎儿倍性状态的一组预期等位基因分布，并将预期等位基因分布与在混合样品中测量的测量等位基因分布的模式进行比较来确定倍性状态，并选择其预期等位基因分布模式与观察到的等位基因分布模式最接近的倍性状态。在一个实施方案中，DNA的混合样品可以以使等位基因偏倚最小化的方式优先富集在多个多态位点。

9. 发明申请

US20140206552A1 METHODS FOR PREIMPLANTATION GENETIC DIAGNOSIS BY SEQUENCING 审中-公开
标题翻译：通过序列预测遗传学诊断的方法
公开(公告)号：US20140206552A1
公开(公告)日：2014-07-24
申请号：US14225356
申请日：2014-03-25
申请人： Natera, Inc.
发明人： Matthew Rabinowitz , Matthew Micah Hill , Bernhard Zimmerman , Johan Baner , Allison Ryan , George Gemelos
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6883 , C12Q2600/156 , G16B20/00 , G16B40/00 , C12Q2537/159 , C12Q2537/161 , C12Q2537/165
摘要： The present disclosure provides methods for determining the ploidy status of an embryo at a chromosome from a sample of DNA from an embryo. The ploidy state is determined by sequencing the DNA from one or more cells biopsied from the embryo, and analyzing the relative amounts of each allele at a plurality of polymorphic loci on the chromosome. In an embodiment, the ploidy state is determined by comparing the observed allele ratios to the expected allele ratios for different ploidy states. In an embodiment, the DNA is selectively amplified at a plurality of polymorphic loci by targeted sequencing. In an embodiment, the mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias.
摘要翻译：本公开提供了用于从胚胎的DNA样品测定染色体上胚胎的倍性状态的方法。倍性状态通过对来自胚胎活检的一个或多个细胞的DNA进行测序，并分析染色体上多个多态性位点处的每个等位基因的相对量来确定。在一个实施方案中，通过将观察到的等位基因比率与不同倍性状态的预期等位基因比率进行比较来确定倍性状态。在一个实施方案中，通过靶向测序在多个多态位点选择性扩增DNA。在一个实施方案中，DNA的混合样品可以以使等位基因偏倚最小化的方式优先富集在多个多态位点。

10. 发明授权

US11746376B2 Methods for amplification of cell-free DNA using ligated adaptors and universal and inner target-specific primers for multiplexed nested PCR 有权
公开(公告)号：US11746376B2
公开(公告)日：2023-09-05
申请号：US16796748
申请日：2020-02-20
申请人： Natera, Inc.
发明人： Matthew Rabinowitz , Matthew Hill , Bernhard Zimmermann , Johan Baner , George Gemelos , Milena Banjevic , Allison Ryan , Styrmir Sigurjonsson , Zachary Demko
IPC分类号： C12Q1/6869 , G16B10/00 , C12Q1/6876 , C12Q1/6844
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q1/6876 , G16B10/00 , C12Q2600/156
摘要： Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式