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1. 发明授权

US06406910B1 Recombination of insertion modified nucleic acids 失效
公开(公告)号：US06406910B1
公开(公告)日：2002-06-18
申请号：US09723424
申请日：2000-11-27
申请人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
发明人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C12N15/8274 , C12N15/10 , C12N15/1027 , C12N15/8216 , C12N15/8289 , Y10T436/143333
摘要： Methods of modulating, tuning and improving hybridization properties and recombination properties of molecules for use in nucleic acid shuffling procedures, relates recombination mixtures and methods of modulating, tuning, improving and evolving splicing of RNAs and proteins are provided.

2. 发明授权

US06413745B1 Recombination of insertion modified nucleic acids 有权
标题翻译：插入修饰的核酸的重组
公开(公告)号：US06413745B1
公开(公告)日：2002-07-02
申请号：US09723520
申请日：2000-11-27
申请人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
发明人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
IPC分类号： C12P2106
CPC分类号： C12N15/8274 , C12N15/10 , C12N15/1027 , C12N15/8216 , C12N15/8289 , Y10T436/143333
摘要： Methods of modulating, tuning and improving hybridization properties and recombination properties of molecules for use in nucleic acid shuffling procedures, relates recombination mixtures and methods of modulating, tuning, improving and evolving splicing of RNAs and proteins are provided.
摘要翻译：调节，调节和改进用于核酸洗牌过程的分子的杂交性质和重组性质的方法涉及重组混合物，并且提供调节，调节，改进和进化的RNA和蛋白质剪接的方法。

3. 发明授权

US06365377B1 Recombination of insertion modified nucleic acids 有权
公开(公告)号：US06365377B1
公开(公告)日：2002-04-02
申请号：US09517933
申请日：2000-03-03
申请人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
发明人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
IPC分类号： C12P1934
CPC分类号： C12N15/8274 , C12N15/10 , C12N15/1027 , C12N15/8216 , C12N15/8289 , Y10T436/143333
摘要： Methods of modulating, tuning and improving hybridization properties and recombination properties of molecules for use in nucleic acid shuffling procedures, relates recombination mixtures and methods of modulating, tuning, improving and evolving splicing of RNAs and proteins are provided.

4. 发明授权

US06358740B1 Recombination of insertion modified nucleic acids 有权
公开(公告)号：US06358740B1
公开(公告)日：2002-03-19
申请号：US09723473
申请日：2000-11-27
申请人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
发明人： Phillip A. Patten , Volker Heinrichs , Willem P. C. Stemmer
IPC分类号： C12N1500
CPC分类号： C12N15/8274 , C12N15/10 , C12N15/1027 , C12N15/8216 , C12N15/8289 , Y10T436/143333
摘要： Methods of modulating, tuning and improving hybridization properties and recombination properties of molecules for use in nucleic acid shuffling procedures, relates recombination mixtures and methods of modulating, tuning, improving and evolving splicing of RNAs and proteins are provided.

5. 发明申请

US20140298538A1 AXMI205 VARIANT PROTEINS AND METHODS OF USE 有权
标题翻译： AXMI205变种蛋白及其使用方法
公开(公告)号：US20140298538A1
公开(公告)日：2014-10-02
申请号：US14233426
申请日：2012-07-27
申请人： Volker Heinrichs , Jayme Williams
发明人： Volker Heinrichs , Jayme Williams
IPC分类号： C12N15/82 , C07K16/12 , C07K14/22 , A01N37/44
CPC分类号： C12N15/8279 , A01N37/46 , A01N63/02 , A01N65/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7, 8, 9, 10, 11, or 12, the nucleotide sequence set forth in SEQ ID NO:4, 5, or 6, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：7,8,9,10,11或12所示氨基酸序列的核酸分子的核酸分子，SEQ ID NO：4所示的核苷酸序列， 5或6，以及其变体和片段。

6. 发明申请

US20110236950A1 GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 有权
标题翻译： GRG23 EPSP合成：组合物和使用方法
公开(公告)号：US20110236950A1
公开(公告)日：2011-09-29
申请号：US12856082
申请日：2010-08-13
申请人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg , Volker Heinrichs
发明人： Laura C. Schouten , Cheryl L. Peters , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N9/00
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

7. 发明授权

US09862965B2 AXMI205 variant proteins and methods of use 有权
公开(公告)号：US09862965B2
公开(公告)日：2018-01-09
申请号：US14233426
申请日：2012-07-27
申请人： Volker Heinrichs , Jayme Williams
发明人： Volker Heinrichs , Jayme Williams
IPC分类号： C12N15/82 , C07K14/325 , A01N37/46 , A01N65/00 , A01N63/02
CPC分类号： C12N15/8279 , A01N37/46 , A01N63/02 , A01N65/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7, 8, 9, 10, 11, or 12, the nucleotide sequence set forth in SEQ ID NO:4, 5, or 6, as well as variants and fragments thereof.

8. 发明授权

US08791326B2 AXMI-150 delta-endotoxin gene and methods for its use 有权
标题翻译： AXMI-150δ-内毒素基因及其使用方法
公开(公告)号：US08791326B2
公开(公告)日：2014-07-29
申请号：US13302428
申请日：2011-11-22
申请人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
IPC分类号： C12N15/63 , C12N15/82 , C12N1/21 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。具体而言，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，即SEQ ID NO：1,3,4,5,6,7， 8，9，10，11或12，以及其变体和片段。

9. 发明申请

US20120073016A1 AXMI-150 DELTA-ENDOTOXIN GENE AND METHODS FOR ITS USE 有权
标题翻译： AXMI-150 DELTA-ENDOTOXIN基因及其使用方法
公开(公告)号：US20120073016A1
公开(公告)日：2012-03-22
申请号：US13302428
申请日：2011-11-22
申请人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
发明人： Kimberly S. Sampson , Daniel J. Tomso , Volker Heinrichs
IPC分类号： A01H1/06 , C12N15/82 , A01H5/00 , C12N1/21 , C12N5/10 , C12N15/32 , C12N15/63
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO:1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments thereof.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包括杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了分离的杀虫核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了具有编码SEQ ID NO：2所示氨基酸序列的核苷酸序列的分离的核酸分子，SEQ ID NO：1，3，4，5，6，7， 8，9，10，11或12，以及其变体和片段。

10. 发明申请

US20100197592A1 VARIANT AXMI-R1 DELTA ENDOTOXIN GENES AND METHODS FOR THEIR USE 有权
标题翻译：变体AXMI-R1 DELTA内毒素基因及其使用方法
公开(公告)号：US20100197592A1
公开(公告)日：2010-08-05
申请号：US12701058
申请日：2010-02-05
申请人： Volker Heinrichs
发明人： Volker Heinrichs
IPC分类号： C07K14/325 , A01N37/44 , A01P7/04
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, nucleic acid molecules encoding variant AXMI-R1 sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
摘要翻译：提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。提供了包含杀虫多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码变体AXMI-R1序列的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。

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