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    • 9. 发明授权
    • Alkaline protease variants
    • 碱性蛋白酶变体
    • US07320887B2
    • 2008-01-22
    • US10836959
    • 2004-04-30
    • Beatrix KottwitzKarl-Heinz MaurerRoland Breves
    • Beatrix KottwitzKarl-Heinz MaurerRoland Breves
    • C12N9/54C12N15/57C12N15/74C11D3/386
    • C12N9/54C11D3/38681
    • The invention relates to novel alkaline protease variants. These variants have, when enumerating the alkaline protease from Bacillus lentus, variations in amino acid position 61, positions 199 and/or 211 and, optionally, at least one modification that contributes to the stabilization of the molecule, said modification preferably being point mutations in positions 3 and/or 4. Particularly preferred are variants S3T/V41/G61A/V199] and S3T/V41/G61A/V1991/L211D of B. lentus alkaline protease. The invention also relates to the possible use of these enzymes in diverse technical processes and, in particular, to detergents and cleansers containing these novel alkaline protease variants.
    • 本发明涉及新的碱性蛋白酶变体。 当列举来自迟缓芽孢杆菌的碱性蛋白酶时,这些变体具有氨基酸位置61,位置199和/或211的变化,和任选地至少一个有助于分子稳定化的修饰,所述修饰优选是点突变 位置3和/或4.特别优选的是变形芽孢杆菌碱性蛋白酶的变体S3T / V41 / G61A / V199]和S3T / V41 / G61A / V1991 / L211D。 本发明还涉及这些酶在不同技术过程中的可能用途,特别涉及含有这些新型碱性蛋白酶变体的洗涤剂和清洁剂。