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1. 发明授权

US4330626A Method of preparing high-activity, low-bacteria, urease enzyme 失效
标题翻译：制备高活性，低细菌，脲酶的方法
公开(公告)号：US4330626A
公开(公告)日：1982-05-18
申请号：US131742
申请日：1980-03-19
申请人： Henry E. Blair , Stanley E. Charm , Richard P. Crowley
发明人： Henry E. Blair , Stanley E. Charm , Richard P. Crowley
IPC分类号： C12N9/80 , C12N13/00
CPC分类号： C12N13/00 , C12N9/80
摘要： In a method of preparing the enzyme, urease, from jack beans, wherein the jack beans are reduced to a fine particle size and the fine particles so reduced constitute urease, the fine jack-bean particles thereafter irradiated for a period of time, the irradiated jack beans then recovered and employed as a source of urease enzyme, the improvement which comprises: irradiating the whole jack beans prior to any size reduction of the seeds of the jack beans, to provide a urease derived from the white seeds of the irradiated whole jack beans having an improved activity level.
摘要翻译：在制备来自千斤顶的酶的尿素酶的方法中，其中千斤顶豆被还原成细粒度，并且如此减少的微粒构成尿素酶，此后将细小的jack豆颗粒照射一段时间，照射千斤顶豆然后回收并用作脲酶的来源，其改进包括：在大豆种子的任何尺寸减小之前照射整个千斤顶豆，以提供衍生自经照射的整个杰克的白种子的脲酶豆类具有改善的活动水平。

2. 发明授权

US4102455A Radioassay kit for method of determining methotrexate 失效
标题翻译：用于测定甲氨蝶呤的方法的放射测定试剂盒
公开(公告)号：US4102455A
公开(公告)日：1978-07-25
申请号：US753852
申请日：1976-12-23
申请人： Stanley E. Charm , Henry E. Blair
发明人： Stanley E. Charm , Henry E. Blair
IPC分类号： G01N33/50 , C12Q1/32 , G01N33/94 , A61B19/00 , A61K43/00
CPC分类号： G01N33/94 , C12Q1/32 , Y10T436/147777 , Y10T436/174614
摘要： A radioassay system for the determination of methotrexate in biological fluids based on the competitive binding of labeled and unlabeled methotrexate to the enzyme dihydrofolate reductase. Samples of unknown methotrexate level are mixed with I.sup.125 labeled methotrexate. A portion of the total methotrexate present is bound by the addition of enzyme, and the unbound methotrexate is removed with charcoal. The level of bound I.sup.125 labeled methotrexate is measured in a gamma counter. To calculate the methotrexate level of the unknown samples, the displacement of bound labeled methotrexate caused by the unknowns is compared to the displacement caused by known methotrexate standards.
摘要翻译：基于标记和未标记的甲氨蝶呤与酶二氢叶酸还原酶的竞争性结合的用于测定生物流体中甲氨蝶呤的放射分析系统。未知甲氨蝶呤水平的样品与I125标记的甲氨蝶呤混合。甲氨蝶呤总量的一部分是通过添加酶结合的，未结合的甲氨蝶呤用木炭除去。在γ计数器中测量结合的I125标记的甲氨蝶呤的水平。为了计算未知样本的甲氨蝶呤水平，将由未知物引起的结合的标记的甲氨蝶呤的位移与由已知的甲氨蝶呤标准引起的位移进行比较。

3. 发明授权

US4043759A Method of determining methotrexate 失效
标题翻译：甲氨蝶呤的测定方法
公开(公告)号：US4043759A
公开(公告)日：1977-08-23
申请号：US650690
申请日：1976-01-20
申请人： Stanley E. Charm , Henry E. Blair
发明人： Stanley E. Charm , Henry E. Blair
IPC分类号： G01N33/50 , C12Q1/32 , G01N33/94 , G01N33/16 , A61K43/00 , G01N31/06
CPC分类号： G01N33/94 , C12Q1/32 , Y10T436/147777 , Y10T436/174614
摘要： A radioassay system for the determination of methotrexate in biological fluids based on the competitive binding of labeled and unlabeled methotrexate to the enzyme dihydrofolate reductase. Samples of unknown methotrexate level are mixed with I.sup.125 labeled methotrexate. A portion of the total methotrexate present is bound by the addition of enzyme, and the unbound methotrexate is removed with charcoal. The level of bound I.sup.125 labeled methotrexate is measured in a gamma ray counter. To calculate the methotrexate level of the unknown samples, the displacement of bound labeled methotrexate caused by the unknowns is compared to the displacement caused by known methotrexate standards.
摘要翻译：基于标记和未标记的甲氨蝶呤与酶二氢叶酸还原酶的竞争性结合的用于测定生物流体中甲氨蝶呤的放射分析系统。未知甲氨蝶呤水平的样品与I125标记的甲氨蝶呤混合。甲氨蝶呤总量的一部分是通过添加酶结合的，未结合的甲氨蝶呤用木炭除去。在γ射线计数器中测量结合的I125标记的甲氨蝶呤的水平。为了计算未知样本的甲氨蝶呤水平，将由未知物引起的结合的标记的甲氨蝶呤的位移与由已知的甲氨蝶呤标准引起的位移进行比较。

4. 发明申请

US20120231483A1 METHOD AND APPARATUS FOR REDUCING LUMINESCENT TEST RESULT INTEREFERENCES (CONTINUATION) 有权
标题翻译：用于减少发光测试结果的方法和装置（续）
公开(公告)号：US20120231483A1
公开(公告)日：2012-09-13
申请号：US13477608
申请日：2012-05-22
申请人： Stanley E. Charm , David R. Legg , Richard T. Skiffington , Robert J. Markovsky
发明人： Stanley E. Charm , David R. Legg , Richard T. Skiffington , Robert J. Markovsky
IPC分类号： G01N21/64
CPC分类号： G01N21/76 , G01N2001/028
摘要： This application involves detecting luminescence. Various methods and devices are described that reduce interference of ambient light, including UV radiation, on test results. Such methods and devices include using UV blocking material and covering test components prior to use.
摘要翻译：该应用涉及检测发光。描述了减少包括紫外线辐射在内的环境光的干扰的各种方法和装置。这些方法和装置包括在使用之前使用UV阻挡材料并覆盖测试组件。

5. 发明授权

US07824878B2 Sensitive method for detecting low levels of ATP 有权
标题翻译：检测低水平ATP的敏感方法
公开(公告)号：US07824878B2
公开(公告)日：2010-11-02
申请号：US12319602
申请日：2009-01-09
申请人： Stanley E. Charm , Cheryl B. Francisco , Robert J. Markovsky , Robert S. Salter , Steven J. Saul
发明人： Stanley E. Charm , Cheryl B. Francisco , Robert J. Markovsky , Robert S. Salter , Steven J. Saul
IPC分类号： C12Q1/66
CPC分类号： C12Q1/008 , C12Q1/66
摘要： Methods are provided for sensitive detection of adenosine triphosphate (ATP) in samples using the luciferin-luciferase reaction. Aspects include using a pH composition that maximizes a signal to noise ratio. The maximum signal to noise ratio can be particularly useful with recombinant luciferase including recombinant Coleoptera luciferase.
摘要翻译：提供了使用荧光素 - 萤光素酶反应敏感检测样品中三磷酸腺苷（ATP）的方法。方面包括使用使信噪比最大化的pH组合物。对于包括重组鞘翅目荧光素酶的重组萤光素酶，最大信噪比对于特别有用。

6. 发明授权

US06475805B1 Method for detection of an analyte 有权
标题翻译：检测分析物的方法
公开(公告)号：US06475805B1
公开(公告)日：2002-11-05
申请号：US09542203
申请日：2000-04-04
申请人： Stanley E. Charm , Richard Skiffington , Robert J. Markovsky , Eliezer Zomer , Steven J. Saul
发明人： Stanley E. Charm , Richard Skiffington , Robert J. Markovsky , Eliezer Zomer , Steven J. Saul
IPC分类号： G01N33543
CPC分类号： G01N33/54366 , G01N33/537 , Y10S435/97 , Y10S435/971 , Y10S436/807 , Y10S436/81
摘要： A test device, system and method, the device composed of an elongated, toothbrush-shaped, transparent, plastic housing and a lateral-flow test strip for the detection of an analyte, such as a beta-lactam in milk, in the housing, the housing having an expansion cavity to receive expanded, liquid-contacted, absorbing material in the test strip, and to control lateral flow rate and times in the test strip.
摘要翻译：一种测试装置，系统和方法，该装置由细长的牙刷状透明的塑料外壳和侧流测试条组成，用于在壳体中检测分析物，例如牛奶中的β-内酰胺，壳体具有膨胀腔以在测试条中接收膨胀的，液体接触的吸收材料，并且控制测试条中的横向流速和时间。

7. 发明授权

US5985675A Test device for detection of an analyte 失效
标题翻译：用于检测分析物的测试装置
公开(公告)号：US5985675A
公开(公告)日：1999-11-16
申请号：US1775
申请日：1997-12-31
申请人： Stanley E. Charm , Richard Skiffington , Robert J. Markovsky , Eliezer Zomer
发明人： Stanley E. Charm , Richard Skiffington , Robert J. Markovsky , Eliezer Zomer
IPC分类号： G01N33/537 , G01N33/543 , G01N37/00 , G01N33/533
CPC分类号： G01N33/54366 , G01N33/537 , Y10S435/97 , Y10S435/971 , Y10S436/807 , Y10S436/81
摘要： A test device, system and method, the device composed of an elongated, toothbrush-shaped, transparent, plastic housing and a lateral-flow test strip for the detection of an analyte, such as a beta-lactam in milk, in the housing, the housing having an expansion cavity to receive expanded, liquid-contacted, absorbing material in the test strip, and to control lateral flow rate and times in the test strip.
摘要翻译：一种测试装置，系统和方法，该装置由细长的牙刷状透明的塑料外壳和侧流测试条组成，用于在壳体中检测分析物，例如牛奶中的β-内酰胺，壳体具有膨胀腔以在测试条中接收膨胀的，液体接触的吸收材料，并且控制测试条中的横向流速和时间。

8. 发明授权

US5374535A Test kit for determination of organophosphate and carbamate pesticides with insect brain material that hydrolyses a 6-substituted d luciferin ester 失效
标题翻译：用于测定有机磷酸盐和氨基甲酸酯农药的测试试剂盒，用于水解6-取代的d荧光素酯的昆虫脑材料
公开(公告)号：US5374535A
公开(公告)日：1994-12-20
申请号：US125935
申请日：1993-09-23
申请人： Eliezer Zomer , Steven Saul , Stanley E. Charm
发明人： Eliezer Zomer , Steven Saul , Stanley E. Charm
IPC分类号： C07D277/68 , C07D277/64 , C12Q1/66 , G01N21/76 , G01N33/567 , G01N33/60 , C12Q1/00 , C12Q1/18 , G01N31/00
CPC分类号： G01N33/567 , C07D277/64 , C12Q1/66 , G01N33/60 , Y10S435/975
摘要： A test kit and a rapid and sensitive method are provided for the multiple detection of organophosphate and carbamate pesticides. The test method employs an insect brain material that hydrolyzes a 6-substituted D luciferin ester, preferably a 6-substituted acetyl D luciferin ester, to produce D luciferin. Hydrolysis is inhibited by the pesticides in a test sample. Luciferase and adenosine triphosphate react with the D luciferin to produce oxyluciferin and emitted bioluminescence. The brain material is initially incubated with a test sample followed by the addition of the ester, luciferase and adenosine triphosphate. The kit contains the brain material, the ester and optionally luciferase and adenosine triphosphate, and may also contain means for incubating, and means for measuring emitted bioluminescence, and a control standard.
摘要翻译：为检测有机磷酸盐和氨基甲酸酯农药提供了一种检测试剂盒和快速灵敏的方法。测试方法采用水解6-取代的D荧光素酯，优选6-取代的乙酰基D荧光素酯的昆虫脑材料以产生D荧光素。水解被测试样品中的农药所抑制。荧光素酶和三磷酸腺苷与D荧光素反应产生氧化荧光素并发射生物发光。脑材料最初与测试样品一起温育，然后加入酯，荧光素酶和三磷酸腺苷。该试剂盒包含脑材料，酯和任选的荧光素酶和三磷酸腺苷，并且还可以含有用于孵育的装置，以及用于测量发射的生物发光的装置和对照标准。

9. 发明授权

US5354663A Microbial inhibition test kit and method 失效
标题翻译：微生物抑制试验盒及方法
公开(公告)号：US5354663A
公开(公告)日：1994-10-11
申请号：US791785
申请日：1991-11-12
申请人： Stanley E. Charm , Eliezer Zomer , Thomas Lieu , Max Gandman , Lea Gandman
发明人： Stanley E. Charm , Eliezer Zomer , Thomas Lieu , Max Gandman , Lea Gandman
IPC分类号： C12M1/00 , C12M1/36 , C12Q1/18 , C12Q1/00 , C12Q1/02 , C12Q1/04
CPC分类号： C12Q1/18 , G01N2035/00366 , G01N2035/00445 , Y10S435/809
摘要： A test kit and method for the detection of antimicrobial drugs at low concentrations in a sample in a test container, such as milk, which includes a Bacillus stearothermophilus (BST) tablet and a medium tablet with nutrients to stress the BST and, optionally, a pH indicator. The method comprises preheating a test sample to destroy natural inhibitors, cooling the sample, adding a BST tablet, rapidly heating to effect synchronization of BST germination, adding a medium tablet, incubating and detecting the presence or absence of the antimicrobial drug. The test apparatus includes optionally a test apparatus for the controlled, sequenced heating and cooling of the test sample.
摘要翻译：用于在测试容器（例如牛奶）中的样品中检测低浓度抗微生物药物的测试试剂盒和方法，其包括嗜热脂肪芽孢杆菌（BST）片剂和具有营养物质的中等片剂以胁迫BST，以及任选地 pH指示剂该方法包括预热试样以破坏天然抑制剂，冷却样品，加入BST片剂，快速加热以实现BST发芽的同步，加入中等片剂，孵育并检测抗微生物药物的存在与否。测试装置可选地包括用于受控的，有序的加热和冷却测试样品的测试装置。

10. 发明申请

US20100144006A1 Sample Concentration Method and Apparatus 审中-公开
标题翻译：样品浓缩方法和仪器
公开(公告)号：US20100144006A1
公开(公告)日：2010-06-10
申请号：US12703953
申请日：2010-02-11
申请人： Stanley E. Charm
发明人： Stanley E. Charm
IPC分类号： C12N13/00 , C12M1/00
CPC分类号： H05B6/806 , G01N1/4022 , G01N2001/4027
摘要： A method and apparatus for controlled concentration of an analyte containing liquid sample. Embodiments include using microwave energy to heat a sample while reducing pressure in a chamber. The combination of reduced pressure and microwave energy can provide sufficient heat to vaporize a portion of the liquid while maintaining analyte integrity. The method and apparatus can increase speed and sensitivity of analyte detection.
摘要翻译：含有液体样品的分析物的受控浓度的方法和装置。实施例包括使用微波能量来加热样品同时减小腔室中的压力。降低压力和微波能量的组合可提供足够的热量来蒸发液体的一部分，同时保持分析物的完整性。该方法和装置可以提高分析物检测的速度和灵敏度。

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